관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

학교 외 교육이 학교 수업에 미치는 영향 : 중등학교 수학교육을 중심으로

곽승곤,박현주 대구대학교 2004 基礎科學硏究 Vol.21 No.1

시상부위에 발생한 단일성 뇌농양 : 증례 보고 및 문헌고찰 Report of Two Cases and Review of the Literature

곽호신,김동규,이상형,정희원,이현구,김현집,한대희 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12

The authors present two cases of solitary thalamic abscess which were intially diagnosed as malignant thalamic glioma. After treatment with steroids and osmotic agents, the patients' clinical conditions improved after two to three days. However, sudden deterioration of consciousness level developed and the CT scan showed acute hydrocephalus with increment of thalamic low-density lesions. We performed extraventricular drainage and obtained CSF containing numerous inflammatory cells. This suggested the possibility that the lesion was a pyogenic abscess. which progressed to ventriculitis, rather than a malignant glioma. Stereotactic aspiration of the lesion confirmed thalamic abscess. These findings and review of previous reports of thalamic abscess suggested that early diagnosis and stereotactic drainage of the thalamic abscess is of primary importance.

育成豚에 있어서 Russian Comfrey의 飼料的 利用價値

郭鍾瀅,鄭鉉丞 진주산업대학교 1968 論文集 Vol.1 No.-

育成豚 飼料로서의 Russian Comfrey의 利用價値를 究明하고자 各各 4頭의 育成豚에 Russian Comfrey를 10%·20%· 30%씩 代置한 飼料와 基本飼料를 給與하여 比較飼養한 結果 다음과 같은 成績을 얻었다. 1)豚의 增體量은 10%代置區가 가장 優秀하였고, 30%代置區가 가장 不良하였으며, 20%代置區는 對照區와 有意差가 없었다. 2)飼料利用性도 對照區에 比하여, 10% 代置區는 優秀하였으며, 20%代置區는 비슷하고, 30%代置區는 不良하였다. 3)Russian Comfrey를 10%∼20% 程度로 代置飼養하면 飼料費가 節約되었으며, 30%代置는 不利하였다. 4)Russian Comfrey에 對한 豚의 嗜好性은 比較的 良好하였으며, Russian Comfrey 30%까지 代置給與하여도 健康上 異常은 별로 없었다. 以上의 結果로 보아 Russian Comfrey를 養豚飼料로 10%∼20% 程度 代置함으로써 有利한 經營을 꾀할 수 있다고 생각된다. The experiment was conducted to determine the feeding value of Russian Comfrey (Symphytum peregreoium) for pigs. Sixteen berkshire gilts were alloted into 4 treatments according to the levels of Russian Comfrey (R.C.) such as 0%, 10%, 20%, and 30% (on air-dried basis) in pig ration. Body weight gain, feed efficiency, economical feeding value and palatability in each treatment were investigated. The experimental results obtained were as follows: 1)In weight gain, the pigs fed 10% R.C. ration showed more gain (P<0.05), but those fed 30% R.C. ration showed less gain (p<0.05) than control group of pigs did. And the pigs fed 20% R.C. ration showed no more gain than the control pigs statistically. 2)Feed efficiency in 10% R.C. ration was also higher than that in any other rations. 3)Feed cost for 1kg. weight gain was lowest in 10% R.C. ration and slightly higher in 30% R.C. ration than that in control ration. 4)From the feed intake it was considered that Russian Comfrey should be considerably palatable to pigs. And from the above results, it was estimated that Russian Comfrey could be supplemented by 20% in the pig ration successfully.

韓國技術人力需給시스템의 動的 特性에 관한 硏究

郭秀一,吳相洛,申鉉麟 東亞大學校 經營問題硏究所 1980 經營論叢 Vol.3 No.-

에너지 및 蛋白質水準이 Broiler의 成長에 미치는 效果

郭鍾瀅,鄭鉉丞,朴成振 진주산업대학교 1969 論文集 Vol.3 No.-

This experiment was conducted to study the effects of level of dietary energy and protein on the growth rate, feed intake and feed conversion of chicken. One hundred and eight male baby chicken of Leghorn was used for 56 days. Starter ration (CP-17, 20,23%; M.E-1,030, 1,200, 1,370㎉/Lb) was fed to chicken for 4 weeks from 3 to 6-week age, and finisher ration (CP-14, 17, 20%; M.E-1,000, 1,215, 1,430㎉/Lb) was fed to chicken for 4 weeks from 7 to 10-weeks age. The results obtained from this study may be summarized as follows; 1) As the level of energy or protein increased in the ration, average daily gain were increased significantly. 2) Amount of feed consumed was significantly higher as the level of dietary protein. In feed conversion, chickens fed higher protein or higher energy were much superior to the chickens fed 10 wer protein or lower energy. 3) There was no influence of dietary energy or protein level on the chicken mortality. 4) Feed cost per kilogram of full body weight gain was increased greatly as the dietary energy level increased. 5) From the above mentioned results of this experiment it was thought that C_1 was profitable level in this study.

종교적 상징성을 줌심으로 본 현대 도시사찰 사례 연구

곽태희,양상현 순천향대학교 부설 산업기술연구소 2004 순천향 산업기술연구소논문집 Vol.10 No.2

This study is to closely examine into depiction of religious symbolism in city temples built after 1990s focusing on Buddhist's dogma and the viewpoint of urban environment. Especially focusing on after 90s, which the ornamented aspect has been actively reflected in architecture on city temples, 10 temples in Seoul, and Kyoungg-gi-do area those have the high distribution of city temples had been selected to be analyzed regarding changes of religious symbolismin morphological, functional, and spatial aspects. Among those the main aspect in changesis the changes of belief system due to the horizontal spatial composition in Danbuljeon.

D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

입원한 정신분열병 환자의 사망원인

곽상곤,서상훈,남정현 漢陽大學校 精神健康硏究 1997 精神健康硏究 Vol.16 No.-

미니동종 조혈모세포 이식에서 T림프구 수용체 단클론성 항체의 이용 가능성 및 혼합 림프구 배양에서 림프구 억제효과에 대한 연구

손상균,박성원,백진호,김동환,정진태,현동우,곽동석,이재태,이규보 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 조혈모세포이식 및 조건화 치료는 원발성 질환을 제거 후 새로운 조혈세포를 이용한 공간을 마련하고 세포의 생착을 이름으로써 치료의 목표를 달성하고자 하나 심한 부작용으로 인한 높은 조기 사망률은 극복하여야 할 점이다. 저독성 조건화 처치를 하면 불충분한 면역세포의 살해로 인한 거부반응이 큰 문제가 된다. 이에 거부반응 및 이식편대숙주반응의 중요한 역할을 하는 림프구에 대한 억제작용을 갖는 단클로성 항체의 생체 내에서 효과를 추측하기 위해 DLA(Dog lymphocyte Antigen) mismatched dog의 말초혈액을 이용한 MLC assay를 시행하였다. 방법: DLA typing의 결과와 비교하기 위해 단클론성 항체 첨가 없이 DLA가 서로 다른 3마리의 말초혈액을 이용하여 서로의 MLC 반응을 비교 실험하였다. T림프구 수용체에 대한 단클론성 항체의 농도에 따른 proloferation 억제효과를 보기 위해 2, 10, 20, 200ug/ml의 농도로 DM5 (antigranulocyte antbody: 음성 대조군)와 비교하였다. 항원에 감작된 림프구에서의 D5에 대한 효과를 보기위해 장기 골수 배양 배지에서 responder를 stimulator 및 D5와 함께 3일간 배양후 세척하고 2일간의 배양후 다시 stimulator로서 재자극 후 5일 뒤에 proliferation assay를 시행하였다. 실제 이식을 시행한 donor 및 recipient의 세포를 분리하여 proliferation assay를 하여 이식 전후를 비교하였다. 결과: T림프구 수용체에 대한 단클론성 항체의 농도를 달리한 실험에서 2, 10 ,20ug/ml 모두에서 같은 농도의 DM5는 억제효과가 없는데 비해상 당한 림프구증식 억제효과가 관찰되었다. In vivo 실험에서 중용량의 방사성 치료 및 면역 억제 요법으로 DLA matched 2마리 모두에서 성공적인 생착을 확인하였다. 공여자의 mismatched sample에 대해 G-CSF를 이용한 가동화 치료전 보다 낮은 proliferation score를 보였다. 결론: T림프구 수용체에 대한 단클론성 항체는 In vitro 실험에서 T림프구의 억제효과가 관찰되었고 동물 이식에서 부작용을 줄이는 저용량의 조건화 치료 후 생착을 유도하는 면역억제제로서 그 가능성을 확인하였고 향후 그 기전에 대한 연구 및 많은 생체 실험이 필요하다고 생각된다. 재생불량성 빈혈 같은 저세포 충실도의 골수 질환이나 과거에 방사선 치료를 심하게 받은 경우는 이미 골수 내 생착을 위한 공간이 마련된 상태이므로 특별한 면역억제 요법이 가능하다면 이러한 저독성의 조건화 처치가 유용하게 응용될수 있을 것으로 전망된다. Background: The objective of this study was to evaluate the effect of anti-TCR monoclonal antibody in mixed lymphocyte culture and as a part of immunosuppressive regimens in the allogeneic stem cell transplantation. Since marrow space is empty in the patients with aplastic anemia or previous radiation and no new space for allograft needs to be created, nontoxic imminosuppressive conditioning regimens should be feasible. These may include monoclonal antibody directed against T cell receptor other surface determinants on T cell. Moncoclonal antibody (MAb) 15.9D5 raised to canine TCR α β was found to induce hyporesponsiveness to allogeneic stimulator cells in primary and secondary mixed lymphocyte culture (MLC). Methods: Anti-TCR monoclonal antibody was tested to evaluate its suppressive effect of lymphocyte activity in vitro compared with anti- granulocyte anti body and used as an immuno-suppressive agent in two recipient dogs. Four different concentration of anti-TCR MAbs was tested in MLC to know the appropriate concentration for suppressing lymphocyte proliferation, and MLC assays were serially done from blood sample of recipient dogs and donor dogs before and after transplantation. Results: Anti-TCR monoclonal antibody had significantly suppressed H3-thymidine uptake in MLC compared with anti-granulocyte antibody(control). In two recipient dogs conditioned with non-myeloablative regimens and MAb, transplanted with DLA- matched donor dogs, successful mobilizing stem cells were less reactive to the stimulator lymphocytes than the lymphocytes from the dogs not treated before. Conclusion: The precise mechanism of immunosuppression induced by anti- TCR MAb needs to be elucidated. G-CSF had a role in suppressing lymphocyte activity of donor dogs and might have a role in preventing GVHD. Non-myeloablative conditioning strategy might be useful in patients with hypoplastic marrows who require immunosuppression only (e. g. aplastic anemia) and already has some spaces for engraftment in the marrow (e. g. irradiated marrows).