Metagenomic Analysis of Fungal Communities Inhabiting the Fairy Ring Zone of Tricholoma matsutake

( Miae Kim1 ),( Hyeok Jun Yoon ),( Young Hyun You ),( Ye Eun Kim ),( Ju Ri Woo ),( Yeong Gyo Seo ),( Gyeong Min Lee ),( Young Ja Kim ),( Won Sik Kong ),( Jong Guk Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10

Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.

The Identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, Using Macroscopic, Microscopic and Physicochemical Methods

Jee-Hyun Yoon,Ha-Yeong Park,Tae-Ju Kim,Seung-Ho Lee,Jung-Hoon Kim,Guem-San Lee,Hong-Jun Kim,Young-Sung Ju 대한한의학회 2014 대한한의학회지 Vol.35 No.4

The Identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, Using Macroscopic, Microscopic and Physicochemical Methods

Yoon, Jee-Hyun,Park, Ha-Yeong,Kim, Tae-Ju,Lee, Seung-Ho,Kim, Jung-Hoon,Lee, Guem-San,Kim, Hong-Jun,Ju, Young-Sung The Society of Korean Medicine 2014 대한한의학회지 Vol.35 No.4

Objectives: This study aimed for establishing the discriminative criteria for Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, which can be confused as 'Mokhyang', because of similar appearances and synonyms. Methods: Morphological characteristics of dried herbal medicines were compared by macroscopic observation. To examine microscopic features of 'Mokhyang', paraffin embedding and the staining by using Ju's method were conducted. Physicochemical experiments were performed using HPLC analysis and antioxidant assay. Results: The types of stem, phyllotaxy and leaf shape were chosen as macroscopic criteria and the size, oil spots, color and root top as well as phloem and central cylinder were compared using microscopic features. The HPLC results showed different amounts of costunolide and dehydrocostuslactone among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. However, Aristolochiae Radix only contained aristolochic acid. The antioxidant assays also showed that Vladimiriae Radix exhibited strongest antioxidant activity followed by Aucklandiae Radix, Inulae Radix and Aristolochiae Radix. Conclusions: These results demonstrate that macroscopic, microscopic and physicochemical methods would be useful tools for the discrimination of 4 kinds of 'Mokhyang'.

폴리 에틸렌 테레프탈레이트 섬유의 改質에 의한 染色性 改良에 關한 硏究

金公朱,姜濚義 全北大學校 1979 論文集 Vol.21 No.-

This paper concerns with the utilization of the treatment of sulfuric acid for improvement of dyeing of poly ethylene terephthalate(P. E. T) fiber. The P. E. T. fiber drafted to various times was treated with 70~74% sulfuric acid at 80~95℃ for 30~120min and neutralized with 2% ammonia. The fiber thus prepared has remarkable advantage in high yields of colours when dyed with C. I. Basic Green 4. The tensile strength of 3.2 times drafted P. E. T. fiber is as high as that of used fiber.

컴퓨터 작업 시 의자 등받이 위치가 근육 활성도에 미치는 영향

김민우,변승진,이경은,정소영,조주영,최원자,최찬양,김경 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 컴퓨터 작업시 의자 등받이 위치가 근육활성도에 미치는 영향을 알아보기 위해 36명의 근골격에 문제가 없는 성인 남녀가 참가하였으며 대상자들은 등받이가 뒤에 있는 의자와 등받이가 없는 의자, 등받이가 앞에 있는 우리들 의자를 무작위로 배정하고 동일한 컴퓨터 작업을 실시하게 하였다. 실험 중, 표면근전도기를 사용하여 의자에 따른 위등세모근, 머리널판근 그리고 척주세움근의 근활성도 변화를 측정하였다. 이 연구의 결과를 종합해보면 등받이가 앞에 있는 의자는 허리 근육의 부담을 줄이지만 목근육에 부담이 늘어나고 등받이가 뒤에 있는 의자와 없는 의자는 허리근육에는 다소 무리가 가지만 목근육에는 부담이 덜하다는 것을 알 수 있었다. 이러한 결과로 환자의 증상에 다라 컴퓨터 작업을 하는 동안 의자 등받이의 위치를 조절하여 치료에 도움이 될 수 있다고 생각되어진다. Objective : In this study, we checked and observed people's change of body muscle and muscle activity who do computer work on different chairs. We set three kind of chairs which one is a stool and another one is the back is fixed, and the other one is the back is fixed in front of chair. Subject : Accounting for 36 health men and women, we divided these people to 3 groups and put them on the three cases(position 1, position 2, position 3). We measured muscle activity of upper trapezius, Splenius Cervics, Erector Spinae from the groups by using surface electromyogram system. Methods : The standard of the electromyogram was 'reference voluntary contraction', and we carried out 'one way anova' to compare muscle activity of three groups. Results : In position 1, reference voluntary contraction of upper trapezius muscle activity was 123.2821%, Splenius Cervics was 141.7526%, and Erector Spinae was 254.5233%. In position 2, reference voluntary contraction of upper trapezius muscle activity was 132.9395%, Splenius Cervics was 141.7526%, and Erector Spinae was 246.6540%. In position 3, reference voluntary contraction of upper trapezius muscle activity was 190.6487%, Splenius Cervics was 270.2332%, and Erector Spinae was 182.1021%. Both upper trapezius and Splenius Cervics muscle activity of position 3 group was higher than the others groups. In position 1 group, Erector Spinae muscle activity was hight than the other groups. And either was position 2 group's(p<0.05). Conclusion : Therefore the chair which is the back is fixed in front is more comfortable for waist muscle but it's not good for neck. And the other chairs are better in waist muscle but not in neck muscle. So far, when we do a computer work, it's prefer to sit on the group 1&2's chairs to reduce neck muscle's stress and sit on group 3's chair to reduce waist muscle's stress.

소각 비산재의 적정처리를 위한 기초연구(Ⅱ) : 용출인자의 영향;Effect of Leaching Parameter

김진범,이우근,심영주 江原大學校 附設 環境硏究所 2000 環境硏究 Vol.17 No.-

본 연구는 소각 비산재의 물리·화학적 특성이 중금속의 용출 거동에 미치는 영향을 알아보고자 수행하였다. 영향인자로는 비산재의 pH, CEC, 입자크기, 연속추출방법에 의해 분류된 중금속의 존재형태 중에서 exchangeable fraction을 고려하였다. KSLT법에 의한 중금속의 용출율은 pH에 크게 의존적이고 원소에 따라 차이를 보였다. 카드뮴과 구리의 용출율은 pH가 증가함에 따라 감소한 반면, 납과 아연은 중성 또는 강알칼리성 조건에서 증가하는 것으로 나타났는데 중금속의 용출 거동이 용해도에 의해 제한됨을 나타내 준다. CEC가 중금속의 용출율에 미치는 영향은 pH와 비슷한 것으로 나타났다. 비산재의 입자크기 중에서 D_(10)은 KSLT법에 의한 중금속의 용출량과 음의 상관성을 나타내었다. Exchangeable fraction의 경우는 비산재의 CEC 값이 40 meq/100g 미만인 시료는 선형관계에 있지만, 그 외의 CEC 값에서는 비선형관계를 보여주었다. Our study was performed to evaluate the effect of leaching parameters including the physicochemical characteristics and the fractionated composition of heavy metals on the release of heavy metals in fly ash discharged from MSWI. Leaching parameters such as pH, CEC, particle size, and exchangeable fraction among the fractional composition classified by sequential extraction procedure are considered. The leaching rate of heavy metal released by KSLT method is largely dependent on the pH of fly ash. The effect of pH on the release of heavy metals is different from elements. It appears that the leaching rate of cadmium and copper decreases with increasing pH, While lead and zinc increases at the condition of neutral or strong alkali condition, which suggests that the leaching of heavy metals are limited by the solubility. It is found that the effect of CEC is similarly to that of pH. D_(10) among the particle size of fly ash is negative correlated with the concentration of heavy metals leached by KSLT method. In the case of exchangeable fraction, the leaching rate of heavy metals is linearly correlated with the exchangeable fraction for the fly ash below 40 meq/100g of CEC, but not related out of the range.

생쥐의 바이러스성 심근염에서 합병증 동반 유무에 따른 조직 및 혈청내 금속단백효소억제자의 발현 차이

김성숙,엄대운,우영주,서재희,허주령,홍영미,최인표 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.3

장거리 선수의 운동시 좌심실 형태에 관한 연구

金福柱,金榮俊,尹榮鶴 東亞大學校附設스포츠科學硏究所 1991 스포츠科學硏究論文集 Vol.9 No.-

The purpose of this study was carried out to investigate changes of the left ventricular dimensions in long distance runners during exercise. The subjects were eight male long distance runners(average age; 21 ±1.04 years, carreer; 7.4 ±2.11 years, height; 170.1 ±4.41cm, body weight; 59.2 ±3.27kg) and ten male non-athletes(average age; 23.2 ±1.16 years, height; 168.3 ±3.65cm, body weight; 61.4 ±3.41kg). Left ventricular dimensions were measured by 2-dimension echocardiography at rest and during 8 min of semisupine, graded bicycle ergometer exercise(0.5kp and 1.0kp) Left ventricular dimension was significantly hyperthrophy in long distance runners than non-athlets. As for dimensions, left ventricular end-diastolic dimension(LVDD), left ventricular end-systolic posterior wall thickness(PWTS), interventricular septum thickness at end-systolic(STS) and amplitude of motion of posterior left ventricular endocardial echo(ENa) increased during exercise in long distance runners and non athletes. Left ventricular end-systolic dimension(LVSD), left ventricular end-diastolic posterior wall thickness(PWTD), interventricular septum thickness at end-diastolic(STD) and ratio of wall thickness to internd radius(h/R) decreased during exercise in long distance runners and non-athletes.

플라이애쉬 콘크리트의 特性에 미치는 굵은 骨材 最大値數의 影響에 관한 實驗的 硏究

김덕현,유영주,이상수,송하영,김을용 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

In this study, the experiment was carried out to investigate and analyze the influence of coarse aggregate's maximum size and Fly Ash replacements ratio on the properties of concrete. The main experimental variables were water/cement ratio 45 and 65(%), maximum size of coarse aggregate 15, 25 and 40(mm), Fly Ash replacements ratio 0, 10, 20(%). According to the test results, the principal conclusions are summarized as follows. 1) The slump and flow of fresh concrete were found to be higher in the order of maximum size of coarse aggregate 15, 25 and 40(mm), also in the order of Fly Ash replacements ratio 0, 10, 20(%). 2) The compressive strength of hardened concrete were found to be lower in the order of maximum size of coarse aggregate 15, 25 and 40(mm). While, Fly Ash replacements ratio increases in age 7days, showed tendency that compressive strength increases together. but, age 28, 56(day) were showed opposite tendency.

치과용 고무인상재의 영구변형 및 압축시 변형률

전창주,김준철,정석민,박영준 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.1

This study investigated the permanent deformation of dental rubber impression materials in order to evaluate their degree of recovery from displacement during removal through undercut area. We also investigated the stain in compression in order to evaluate the materials' flexibility during removal from the month, or during removal of the cast from the impression. Additional silicone, polyether, and polysulfied rubber impression materials were tested for their properties of permanent deformation and strain in compression according to the testing methods of the ADA specificaltion no. 19. 1.The permanent deformation values were as follows: polysulfide (2.25%), polyether (2.14%), and additional silicone (0.28∼1.21%, 2.38% for Perfect F). Examix, Express and Provil, which are additional silicone rubbers, showed lower permanent deformation values of 0.29%, 0.28% and 0.34%, respectively. Perfect F, although it is an additional silicone rubber, showed the highest permanent deformation value (2.38%) within the tested materials. 2.The strain in compression values were as follows: polysulfide (10.63%), additional silicone (2.88∼10.09%), and polyether (2.38%). The additional silicone rubber samples showed various values. Contrary to our expectations, it is also notable that Vinylate, which is used when taking an one-step impression, had higher stain in compression value (10.09%) than the light body impression materials even though it is classified as a heavy body material. Express (a.s.) had a low strain in compression (2.88%) which is similar to that (2.83%) of Impregum F (p.e.). In light body rubber materials, Permlastic had the highest value (p.s. 10.63%), followed by Perfect F (a.s. 7.38%), Correct VPS (a.s. 7.13%), Examix (a.s. 6.04%), Provil (a.s. 3.82%) and Express (a.s. 2.88%). Although the tested materials showed various values, all of them satisfied the requirements of A.D.A. Specification No. 19, which require that permanent deformation be less than 2.5%, and strain in compression be between 2∼20%.