肺炎 誘發菌의 生育을 抑制하는 韓藥材 探索에 關한 硏究

정병운,서운교,정지천,한영환 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

韓藥材 중 肺炎의 치료효과가 기대되는 淸熱化痰, 止咳平喘藥을 중심으로 23種의 藥材를 사용하여 肺炎을 유발하는 K. pneumoniae, S. pyogenes 및 S. pneumoniae 세균의 생육을 억제하는 藥材를 탐색하고, 탐색된 藥材의 추출물에 대한 세균의 最小生育抑制濃度를 측정하였다. 韓藥材의 水溶性 추출물 중 黃連, 胡黃蓮, 敗醬, 黃芩이 K. pneumoniae에서, 黃連이 S. pyogenes에 대해서 생육억제 효과가 있었으며, S. pneumoniae에 대한 比較沮止環의 直徑은 黃連, 鳥梅, 五味子, 黃芩이 우수하였으나 黃連 추출물 사용시 병원균 모두에서 生育沮止環의 크기가 가장 크게 나타났으며 對照群로 사용된 大腸菌과 枯草菌에서도 어느 정도의 抗細菌 효과를 볼 수 있었다. 에탄올 추출물에서는 敗醬, 黃芩, 五味子, 鳥梅가 K. pneumoniae 세균의 생육을 억제하였으며, S. pyogenes 세균은 黃連, 五味子, 鳥梅, 馬兜鈴찾, 黃芩에 의하여 생육이 억제되어 黃連은 水溶性 및 에탄올 溶解性 주출물에서 모두 우수한 抗細菌 효과를 보여주고 있다. 각 세균에 대한 最小生育沮止濃度(MIC)는 K. pneumoniae 세균일 경우 黃連 및 黃芩의 水溶性 추출물과 鳥梅 및 敗醬의 에탄올 溶解性 추출물이 유의성을 보였으며, S. pyogenes 세균은 黃連의 水溶性 및 에탄올 溶解性 추출물과 鳥梅의 에탄올 溶解性 추출물, S. pneumoniae 세균은 黃連과 鳥梅의 水溶性 및 에탄올 溶解性 주출물에서 유의성이 나타났다. 이상의 결과를 통하여 黃連, 五味子, 鳥梅, 黃芩, 胡黃蓮, 敗醬의 水溶性 및 에탄올 溶解性 추출물이 肺炎 誘發菌f K. pneumoniae, S. pyogenes, S. pneumoniae에 對하여 우수한 生育抑制 作用을 나타냄을 알 수 있었다. The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against pulmonary disease-causing Klebsiella pneumoniae, Streptococcus pyogenes, and Streptococcus pneumoniae. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extracts of Coptis japonica, Scutellaria baicalensis and Picrorrhiza kurroca showed the antibacterial activity against K. pneumoniae and that of C. japonica against S. pyogenes. The antibacterial activities of C. japonica, Prunus mume, Schizandra chinesis, Scutellaria baicalensis were also found against S. pyogenes. When C. japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little activity against B. subtilis and E. coli as a control. 2. The ethanol-soluble extracts of Patrinia scabriosaefolia, P. mume, S. baicalensis, S. chinensis showed the antibacterial activity against K. pneumoniae and those of S. baicalensis, C. japonica, S. chinensis P. mume against S. pyogenes and S. pneumoniae. However, those extract showed a little antibacterial activity against B. subttilis and E. coli except for that the extract of C. japonica showed comparatively high growth inhibition of B. subtilis. 3. Among the medicinal herbs tested, the water- and ethanol extracts of C. japonica showed very extcellent antibacterial activity against the pathogenic bacteria and controls. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against K. pneumoniae were 10 ㎎/㎖ and 22 ㎎/㎖, respectively. The MICs of the ethanol-soluble extracts of P. mume and P. scabriosaefolia were 5 ㎎/㎖ and 20 ㎎/㎖, respectively. 5. For the MICs against S. pyogenes, C. japonica showed 15 ㎎/㎖ with the water-soluble extract and P. mume and C. japonica with the ethanol-souble extract did 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. 6. For the MICs against S. pneumoniae, C. japonica and P. mume with the water- and ethanol-souble extract showed 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. As a result, the highest antibacterial activity was found in the water- and ethanol-soluble extracts of C. Japonica against pulmonary disease-causing bacteria, K. pneumoniae, S. pyogenes, and S. pneumoniae. Also, the water- and ethanol-soluble extracts of S, chinensis, P. mume, S. baicalensis and P. kurrooa showed high antibacterial activities.

보문 : 순천만에 자생하는 염생식물의 뿌리로부터 분리된 내생진균의 유전적 다양성

서영교 ( Yeong Gyo Seo ),김미애 ( Mi Ae Kim ),유영현 ( Young Hyun You ),윤혁준 ( Hyeok Jun Yoon ),우주리 ( Ju Ri Woo ),이경민 ( Gyeong Min Lee ),김종국 ( Jong Guk Kim ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.1

Parameter Evaluation of a Smooth Elasto-plastic Cap Model

서영교(Seo Yeong Gyo) 한국지반공학회 2004 한국지반공학회논문집 Vol.20 No.2

In this paper, the method of parameter estimation of a mathematical constitutive model known as the smooth elasto-plastic cap model is studied. To predict the response of the real soil using this model, the eight parameters describing the constitutive equations have to be determined. First, experimental data are obtained from simple laboratory experiments such as one dimensional confined compression test in a consolidometer and drained triaxial compression test with the Ottawa sand for the reference value. Then, the numerical experiments are performed in the cap model with initial guessed parameters. The optimization method is utilized to fit the model response to experimental data by minimizing the error between the laboratory and numerical responses. Special attention is given to the parameter estimation procedure of numerical triaxial test due to the difficulty of the lateral strain measurements.

Changes of Nutritional Components, Polyphenols, and Antioxidant Activities of Domestic Bamboo Tree (Sasa Coreana Nakai) Leaves Fermented With Bacillus Subtilis

Han-Gyo JO,Dasong KIM,Yeong-Jin SEO,Hyun-Jae SHIN 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.4

Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of <i> Diospyros kaki</i> Fruit and <i> Citrus unshiu</i> Peel

Kim, Gyo-Nam,Shin, Mi-Rae,Shin, Sung Ho,Lee, Ah Reum,Lee, Joo Young,Seo, Bu-Il,Kim, Min Yeong,Kim, Tae Hoon,Noh, Jeong Sook,Rhee, Man Hee,Roh, Seong-Soo Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-

<P>Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme of<I> Diospyros kaki</I> fruit and<I> Citrus unshiu</I> peel mixture extract (PCM) was evaluated<I> in vitro</I> and its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed mice<I> in vivo</I>. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC<SUB>50</SUB> value of 507.01 <I>μ</I>g/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (<I>p</I> < 0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.</P>

자체제작 도플러 팬텀의 활용 가능성 평가(교육 및 실습용)

유은교(Eun-Gyo Yu),양세영(Se-Yeong Yang),한아름(A-Reum Han),이제운(Je-Un Lee),최우진(Woo-Jin Choi),정선희(Sun-Hee Jung),서애실(Ae-Sil Seo),이후민(Hoo-Min Lee),김현주(Hyeon-Ju Kim) 대한초음파의료영상학회 2023 대한초음파의료영상학회 학술대회 초록집 Vol.7 No.1

해저지반과 해저케이블 매설

서영교,이광열,하광수,김태형,Seo, Yeong-Gyo,Lee, Gwang-Yeol,Ha, Gwang-Su,Kim, Tae-Hyeong 한국지반공학회 2012 지반 : 한국지반공학회지 Vol.28 No.3

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

Distribution, Characterization, and Diversity of the Endophytic Fungal Communities on Korean Seacoasts Showing Contrasting Geographic Conditions

( Young-hyun You ),( Jong Myong Park ),( Yeong Gyo Seo ),( Woong Lee ),( Myung-suk Kang ),( Jong-guk Kim ) 한국균학회 2017 Mycobiology Vol.45 No.3

This study analyzed the distribution of endophytic fungi in 3 coastal environments with different climatic, geographical, and geological characteristics: the volcanic islands of Dokdo, the East Sea, and the West Sea of Korea. The isolated fungal endophytes were characterized and analyzed with respect to the characteristics of their host environments. For this purpose, we selected common native coastal halophyte communities from three regions. Molecular identification of the fungal endophytes showed clear differences among the sampling sites and halophyte host species. Isolates were also characterized by growth at specific salinities or pH gradients, with reference to previous geographical, geological, and climate studies. Unlike the East Sea or West Sea isolates, some Dokdo Islands isolates showed endurable traits with growth in high salinity, and many showed growth under extremely alkaline conditions. A smaller proportion of West Sea coast isolates tolerate compared to the East Sea or Dokdo Islands isolates. These results suggest that these unique fungal biota developed through a close interaction between the host halophyte and their environment, even within the same halophyte species. Therefore, this study proposes the application of specific fungal resources for restoring sand dunes and salt-damaged agricultural lands and industrialization of halophytic plants.

Melatonin이 Cisplatin에 의한 신장 독성에 미치는 영향

최혜정 ( Choe Hye Jeong ),신영호 ( Sin Yeong Ho ),문교철 ( Mun Gyo Cheol ),송대규 ( Song Dae Gyu ),김인철 ( Kim In Cheol ),서상혁 ( Seo Sang Hyeog ),곽춘식 ( Gwag Chun Sig ),장은주 ( Jang Eun Ju ),김현철 ( Kim Hyeon Cheol ) 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.2

목 적 : Cisplantin은 항암제로 널리 사용되나 활성산소를 생성하여 신장 기능 손상 등의 부작용이 있는 것으로 알려져 있긴 강력한 항산화제인 melatonin은 cisplatin에 의한 산장독성을 줄일 수 있을 것이다. Cisplatin에 의한 신장 독성에 대하여 melatonin의 방어 효과를 알아보고자 하였다. 방 법 : 실험군을 대조군인 제 1군, cisplatin 투여군인 제 2군 및 cisplatin과 melatonin을 투여한 제 3군으로 나눈 후 신장 조직의 형태학적 변화와 더불어 산화적 손상에 대한 연구를 수행하였으며 과산화수소, malondialdehyde 및 항산화효소의 활성도를 측정하였다. 결 과 : 혈청 BUN (p<0.001), creatinine 농도 (p<0.001) 및 신장의 과산화수소 생성량은 제 1군에 비해 제 2군에서 증가하였고, 제 2군에 비해 제 3군에서는 감소되었으며, 활성산으로 인한 손상 표지자인 malondialdehyde 농도도 같은 경향을 나타내었다. Creatinine 청소는 제 1군에 비해 제 2군에서 감소를 보였고, 제 2군에 비해 제 3군에서는 증가되었다. 신장의 superoxide dismutase 활성도는 제 1군에서 제 2군에 비해 감소하였고 catalase 활성도는 제 1군에 비해 제 2군에서 증가되었으며, 제 1군에 비해 제 3군에서는 더욱 증가되었다. Glutathione peroxidase는 통계학적으로 의미 있는 변화가 없었다. 또한 광학 현미경 소견을 보면 제 2군의 사구체에서는 특이한 변화가 없었으나, 근위 곱슬 세관은 심한 괴사를 나타내었고, 제 3군의 근위 곱슬 세관에서 중등도의 괴사를 나타내었으며 제 2군보다 경한 변화를 보였다. 결 론 : Cisplatin은 신장에서 superoxide dismutase 활성도 증가와 catalase의 활성도를 저하시켜 과산화수노의 생성을 유도하여 신장 손상을 유발하는 것으로 생각되며, melatonin은 superoxide dismutase 활성도 증가와 catalase 활성도 증가를 유도하여 cisplatin에 의해 유발된 신장 손상을 줄이는 것으로 생각된다. Background : Cisplatin (CP), an antitumor agent widely used in the treatment of cancers, has nephrotoxicity. This side effect is closely related to oxidative stress. In the present study, we attempted to reduce CP-induced nephrotoxicity in rats by administering melatonin, an antioxidant. Methods : Male Sprague-Dawley rats were divided into different groups and were treated as follows : ⑴ saline control; ⑵ CP (16 mg/kg, i.p.); ⑶ CP plus melatonin (10 mg/kg, i.p.). The rats were sacrificed at the 6th day after CP treatment. To evaluate renal damage, BUN, serum creatinine, creatinine clearance and microscopic examination were done. Hydrogen peroxide which is one of the oxygen free radicals, and malondialdehyde which is known as a marker of the oxygen free radical mediated injury, and the activities of the antioxidant enzymes such as superoxied dismutase, catalase, and glutathione peroxidase were also measured. Results : CP-treated rats showed the increase of BUN, serum creatinine, malondialdehyde, hydrogen peroxide and superoxide dismutase (SOD) in kidney. And CP-treated rats also showed the decrease of creatinine clearance and catalase levels. CP-treated rats showed severe tubular necrosis in proximal convoluted tubules under the light microscopic examination. The light microscopic finding and all of the parameters except SOD were restored in the rats injected with CP plus melatonin than those with CP alone. SOD level was higher in the rats injected with CP plus melatonin than that with CP alone. Conclusion : These results suggest that melatonin suppresses CP-induced nephrotoxicity by suppressing the production of reactive oxygen species via the activation of SOD and catalase. (Korean J Nephrol 2004;23(2):205-212)