Cloning and mRNA Expression Analysis of the Gene Encoding Phenylalanine Ammonia-Lyase of the Ectomycorrhizal Fungus Tricholoma matsutake

( Hyeok Jun Yoon1 ),( Young Hyun You ),( Ye Eun Kim ),( Young Ja Kim ),( Won Sik Kong ),( Jong Guk Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

The ectomycorrhizal fungus Tricholoma matsutake grows symbiotically with Pinus densiflora. Phenylalanine ammonia-lyase (E.C. 4.3.1.24) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. The role of fungal phenylalanine ammonia-lyase, however, has not been clear until now. In this study, the gene encoding phenylalanine ammonia-lyase (PAL), which was isolated from T. matsutake, was cloned and characterized. The PAL gene (tmpal) consists of 2,160 nucleotides, coding for a polypeptide containing 719 amino acid residues. The deduced amino acid sequence of tmpal from T. matsutake shows high identity (70%) with that from Laccaria bicolor. Comparative analysis of the PAL genes among T. matsutake and other species of the class Agaricomycetes showed that both active sites and binding sites were significantly conserved among these genes. The transcriptional analysis of the PAL gene revealed a differential gene expression pattern depending on the developmental stages (mycelium, primordium, stipe, pileus, and gills) of T. matsutake. These results suggest that the PAL gene in T. matsutake plays an important role in multiple physiological functions.

Adjuvant Cytokine-Induced Killer Cell Immunotherapy for Hepatocellular Carcinoma: A Real-World Experience from Two Large-Volume Centers in Korea

( Jun Sik Yoon ),( Byeong Geun Song ),( Jeong-hoon Lee ),( Hyo Young Lee ),( Sun Woong Kim ),( Young Chang ),( Eun Ju Cho ),( Su Jong Yu ),( Dong Hyun Sinn ),( Yoon Jun Kim ),( Joon Hyeok Lee ),( Jung 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Several randomized controlled trials have shown that adjuvant immunotherapy with autologous cytokine-induced killer (CIK) cells prolongs recurrence-free survival (RFS) after curative treatment for hepatocellular carcinoma (HCC). We investigated the efficacy of adjuvant immunotherapy with activated CIK cells in real-world clinical practice. Methods: A total of 370 patients with stage I or II HCC who underwent curative surgical resection or radiofrequency ablation at Seoul National University Hospital or Samsung Seoul Medical Center were included in this study. Among them, 71 patients received CIK cell immunotherapy after curative treatment and 299 patients did not. Propensity score matching with a 1:1 ratio was conducted to avoid possible bias and 64 pairs of matched patients were generated. The primary endpoint was RFS and secondary endpoints included overall survival. Results: After a propensity score matching, there was no statistical difference in variables including treatment modalities, HCC stage, presence of cirrhosis, alpha-fetoprotein level between the two groups. The median follow-up duration was 16.0 months (interquartile range, 7.8 - 27.0). The immunotherapy group did not reach median RFS and the control group showed 33.5 months of median RFS (P=0.001 by log-rank test). CIK cell adjuvant therapy reduced the risk of tumor recurrence or death by 65% (hazard ratio [HR], 0.35; 95% confidence interval [CI], 0.18-0.68, P=0.002, Figure 1). No patient (0%) in the immunotherapy group and 5 patients (7.8%) in the control group died during the study period. However, the difference in OS between two groups failed to reach statistical significance (P=0.060 by Firth’s method, Figure 2). Multivariable Cox regression analysis showed that immunotherapy was an independent predictor for HCC recurrence (adjusted HR, 0.16; 95% CI, 0.04-0.65; P=0.010). Conclusions: The adjuvant immunotherapy with autologous CIK cells prolongs RFS in patients with HCC after curative therapy in a real-world setting.

폴리프로필렌/셀룰로오스 나노 섬유 복합체의 열적 및 기계적 물성

윤혁준(Hyeok Jun Yoon),길보민(Bo Min Gil),이종혁(Hyeok Lee),박정은(Jeong Eun Park),임종휘(Jonghwi Lim),조명준(Myeong Jun Jo),정경호(KyungHo Jung),위정재(Jeong Jae Wie) 한국고분자학회 2020 폴리머 Vol.44 No.3

최근, 가장 풍부한 천연고분자 중 하나인 셀룰로오스를 사용하기 위해 제지산업, 분리막, 보강재 등에 대한 연구가 진행되고 있다. 본 연구에서는 소비자 포장재, 자동차 산업 등 산업에서 가장 많이 쓰이고 있는 플라스틱 중 하나인 폴리프로필렌(polypropylene, PP)의 기계적 물성을 증가시키기 위해 셀룰로오스 나노 섬유(cellulose nanofiber, CNF)를 사용해 복합체를 제조했다. 무극성인 폴리프로필렌에 극성인 CNF를 분산시키기 위해 무수말레인산 폴리프로필렌(maleic anhydride polypropylene, MAPP)을 상용화제로 첨가했다. CNF가 첨가되면서 인장강도와 굴곡강도는 크게 향상됐으며 충격강도는 감소했다. SEM 분석 결과 PP/MAPP 매트릭스와 CNF 사이의 상호작용이 확인됐고 인장강도와 굴곡강도는 매트릭스와 CNF 간의 상호작용이 크게 작용해 증가한 반면 충격강도는 기공과 CNF로 인한 응력집중에 의해 감소함을 확인했다. TGA 분석결과 CNF가 열적으로 불안정하기 때문에 CNF의 함량이 증가할수록 복합체의 열적 안정도는 낮아졌다. 또한 높은 함량의 CNF 샘플에서 결합수로 인해 기공이 증가하는 현상을 관측했다. Recently, one of the most abundant natural polymers in the earth, cellulose has been studied in various fields. In this study, polymer composites were produced through introduction of cellulose nanofiber (CNF) into polypropylene (PP) which is one of the most commercially utilized polymer for enhanced mechanical properties. Also, maleic anhydride polypropylene (MAPP) was added to PP/CNF composites for compatibility improvement between non-polar PP and polar CNF. Both tensile strength and flexural strength were increased with the addition of CNF, but, on the other hand, impact strength was decreased. As a result of SEM analysis, the interaction between PP/MAPP matrix and CNF was created, and the tensile and flexural stress were influenced by the interaction between PP/MAPP matrix and CNF. Impact stress confirmed that it decreases by pore and stress concentration by CNF. The TGA analysis confirmed that composites are thermally unstable by CNF. Therefore, as CNF contents increased, the initial and final decomposition temperature of the composites are decreased. We also observed increase of porosity because of bound water in the case of high CNF contents.

RFID system의 채널 에러 환경에서 DFSA 알고리즘의 프레임 크기 설정

윤준혁(Jun-Hyeok Yoon),엄준봉(Jun-Bong Eom),이태진(Tae-Jin Lee) 한국통신학회 2008 한국통신학회 학술대회논문집 Vol.2008 No.11

국내 전력시장의 직접구매제도 개선방안에 관한 연구

윤혁준(Hyeok Jun Yoon) 에너지경제연구원 2021 에너지경제연구 Vol.20 No.2

한국전력시장에서 복합발전기의 운전조합별 비용함수의 계통한계가격(SMP) 결정메커니즘 영향에 관한 연구

윤혁준 ( Hyeok Jun Yoon ) 한국환경경제학회·한국자원경제학회(구 한국환경경제학회) 2021 자원·환경경제연구 Vol.30 No.1

우리나라 전력시장에서 한계비용 기반의 가격결정메커니즘에 관한 논의가 이루어질 때 마다 한계발전기의 수익 감소는 가장 큰 장애물로 인식되었다. 하지만 수익감소는 한계비용 기반의 가격결정메커니즘의 한계점이 아니라 시장개설 초기 CBP체제의 단기 운영 계획, 발전계획프로그램의 기능 부재, 성능시험의 구조적 결함이 결합된 문제이다. 다조합 복합발전기에 운전조합별 비용함수를 적용할 경우 고출력구간에서 한계비용이 평균비용을 초과할 수 있으며, 이것은 비용함수를 산정하는 방식에 따라 가격결정메커니즘 제도개선을 가로막았던 구조적인 장애물을 극복할 수 있는 기회를 제공할 수 있다. 실시간 시장, 계통운영보조서비스 시장 등 재생에너지 확대를 수용하기 위한 전력시장 개선 및 DR, ESS 등 새로운 자원들의 시장참여 확대 등 향후 전력시장의 복잡성은 더욱 증가할 것으로 전망된다. 전력시장이 복잡해질수록 합리적인 가격신호를 제공하는 것이 바로 전력시장의 가장 중요한 역할이며 한계비용 기반의 가격결정메커니즘이 바로 전력시장 선진화의 출발점이 될 것이다. It has been recognized that implementing the marginal price mechanism to CBP is not acceptable due to the lack of revenue of the marginal generators. This study shows that it is not the problem of marginal price mechanism but the structural problems originated by the suspension of restructuring, the technical limits of RSC program and inaccuracy of the generation cost estimation method. This study explains the method to calculate the cost function in operating modes of the CC generators and proposes the modeling for the CC generators in RSC program. To implementing the cost function in operating modes could give an opportunity to change the price setting mechanism from average to marginal cost. The price setting mechanism based on the marginal cost will be one of the main points to provide the right price signals and to introduce a real-time and A/S markets to prepare the energy transition era.

등받이 의자와 좌변기에서 스마트폰 사용 시 목세움근, 위등세모근, 척추세움근의 근활성도 비교

김준혁 ( Jun-hyeok Kim ),강슬기 ( Seul-gi Kang ),김민주 ( Min-joo Kim ),민유진 ( Yu-jin Min ),백숙향 ( Suk-hyang Baek ),장윤호 ( Yoon-ho Jang ),주미정 ( Mi-jung Ju ),최가람 ( Ga-ram Choi ),홍성화 ( Sung-hwa Hong ),권혁규 ( Hyeok-g 대한신경치료학회 2018 신경치료 Vol.22 No.2

Purpose The usage of a smartphone in the life have been increased because of the convenience of a smartphone. In addition, the usage of a smartphone is increased in the restroom. The use of smartphone in the toilets have affected on large intestine such as constipation and hemorrhoids. However, little is known about musculoskeletal system. Therefore, the Purpose of this study was to examine the effect of using smarphone in the restroom on muscle activity of cervical erector spineae, upper trapezius, and lumbar erector spinae in the able-bodied people. Methods 28 healthy subjects were recruited for this study. Electromyographic signals of both cervical erector spinae, upper trapezius and lumbar erector spinae were acquired during sitting on the general chair and toilet with using a smartphone. Paired t-test was used for determination of differences in the muscle activation between general chair and toilet. The significant level of the p value was set at 0.05. Results In cervical erector spinae and upper trapezius, no significant difference was observed between the general chair and toilet (p>.05). In contrast, regarding lumbar erector spinae, using a smartphone on the toilet showed significant difference compared to on the general chair (p<.05). Conclusion We found that the sitting on the toilet during using a smartphone showed higher muscle activation of lumbar erector spinae than the sitting on the general chair. Therefore, using a smartphone on the toilet could affect the musculoskeletal of the lower back.

Impact of Interferon-Based Treatment on Quality of Life and Work Related Productivity from the Korean Cohort in the MOSAIC Study

( Sang Hoon Ahn ),( Won Hyeok Choe ),( Yoon Jun Kim ),( Jeong Heo ),( Dorota Latarska-smuga ),( Jiho Kang ),( Seung Woon Paik ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Chronic Hepatitis C Virus (HCV) infection increases the risk for progressive liver disease, hepatocellular carcinoma and negatively impacts the patient’s quality of life. HCV treatment is evolving with direct acting antivirals but IFN based therapy has been the standard of care for many years and remains available in some countries. The MOSAIC study aims to characterize patients with chronic HCV infection and assess the impact of IFN-containing treatment on health-related quality of life, work related productivity and health care utilization. Methods: MOSAIC is an international prospective multicenter observational study that has been conducted in 20 countries. Consecutive patients with chronic HCV infection were enrolled and those who initiated an IFN based regimen were prospectively followed for 48 weeks. We report results from the Korean cohort Results: 100 patients were enrolled: 86 were treatment naïve and 14 were treatment experienced. 33 patients initiated an IFN based regimen: 6 patients started IFN + RBV, 26 patients started Peg-IFN + RBV, none started Peg-IFN + RBV + DAA and 1 patient received other treatment. Among the treated cohort, demographic and disease characteristics were the following: the mean age was 54.5 years; 14 patients were male. 14 had minimal or no fibrosis, 2 portal fibrosis, 3 bridging fibrosis and 6 patients suffered from cirrhosis. HCV Genotype distribution was as follows: genotype 1: 11; genotype 2: 19 and genotype 3: 3. Table 1 describes the results at baseline and changes over 4, 12 and 48 weeks and end-of-treatment (EOT) for the quality of life and work productivity outcome measures (EQ-5D-5L, HCV-PRO and WPAI). Conclusions: Results from the Korean cohort of the MOSAIC study show a moderate trend for deterioration of health-related quality of life and work productivity associated with IFN based treatment for patients with chronic HCV infection during treatment period. Acknowledgements: The design, study conduct, analysis, and financial support of MOSAIC study were provided by AbbVie. AbbVie participated in the interpretation of data, review, and approval of the content of the abstract. All authors had access to all relevant data and participated in writing, review, and approval of this abstract. Medical writing support was provided by Olivier Van de Steen of Medeor-consulting, funded by AbbVie. Disclosures: Sang Hoon Ahn: served as an advisor and lecturer for Bristol-Myers Squibb, Gilead Sciences, F.Hoffmann-La Roche, Merck, AbbVie, and has received unrestricted grants from Bristol-Myers Squibb, Gilead Sciences, and F. Hoffmann-La Roche for investigator- initiated trials Won Hyeok Choe: Nothing to disclosure Yoon Jun Kim: Nothing to disclosure Jeong Heo: received a grant from GSK; Research support from BMS, and Roche; Advisor for Abbvie, BMS, Gilead Sciences, Pharma Essentia, SillaJen, and Johnson & Johnson. Dorota Latarska-Smuga, Jiho Kang: are employees of AbbVie, Inc. and may hold stock or stock options. Seung Woon Paik: received grant and research support from AbbVie, BMS, Gilead, GSK, Merck, Novartis, and Roche

Two new phenolic compounds from the leaves of <i>Alnus sibirica</i> Fisch. ex Turcz.

Kim, Manh Heun,Park, Kwan Hee,Kim, So Ra,Park, Kwang Jun,Oh, Myeong Hwan,Heo, Jun Hyeok,Yoon, Ki Hoon,Yin, Jun,Yoon, Kyu Hyung,Lee, Min Won TaylorFrancis 2016 NATURAL PRODUCT RESEARCH Vol.30 No.2

<P>Two new phenolic compounds, 4-<I>O</I>-glucopyranosyl-5-<I>O</I>-caffeoylshikimic acid (<B>1</B>) and 2,3-digalloyl oregonin (<B>2</B>), were isolated along with eight known phenolic compounds (<B>3</B>–<B>10</B>) from an 80% acetone extract of <I>Alnus sibirica</I> leaves. The chemical structures of these compounds were elucidated using 1D/2D nuclear magnetic resonance and high resolution-MS. The anti-oxidative activities of these compounds were determined by assaying their 1,1-diphenyl-2-picrylhydrazyl radical and nitroblue tetrazolium superoxide anion scavenging activity. All of the isolated phenolic compounds (<B>1</B>–<B>10</B>) exhibited potent anti-oxidative activities. In particular, <B>2</B> and 4, which are diarylheptanoids, and <B>10</B> which is ellagitannin exhibited excellent anti-oxidative activities with almost the same potency as that of the positive controls <I>L</I>-ascorbic acid and allopurinol.</P>

소아 신우요관이행부 폐쇄의 외과적 치료

서혁준,정성광,박윤규 大韓泌尿器科學會 1997 Korean Journal of Urology Vol.38 No.11