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Genetic diversity of super-sweet corn inbred lines using SSR and SSAP markers
Woo Ri Ko,Hong-Jib Choi,Kyu Jin Sa,Ju Kyong Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In this study we evaluate the informative and efficiency of Simple Sequence Repeat (SSR) and Sequence Specific Amplified Polymorphism (SSAP) markers for genetic diversity, genetic relationship and population structure among 87 super sweet corn inbred lines generated by different origins. The SSR showed relatively higher level of the average gene diversity and shannon’s information index value than that of the SSAP. To assess genetic relationship and to characterize among 87 super sweet corn inbred lines using the SSR and SSAP markers. The dendrogram using SSR marker divided into nine groups of clusters were observed at the genetic similarity value 53.0%. For SSAP marker, Total three main clusters were confirmed in genetic similarity value at 50.8%. Result of combine data for SSR and SSAP markers showed six subgroup were detected in genetic similarity at 53.5%. To confirm population structure, the total 87 super sweet corn inbred lines were divided into groups I, II and admixed group based on membership probability 0.8 for SSR and SSAP markers. However population structure using combine data was K=3 and divided into group I, II, III and admixed group. This study has demonstrated the comparative analysis of SSR and SSAP for the study of genetic diversity and the genetic relationship for super sweet corn inbred lines. Thus, the results of this study will be useful to maize breeding programs in Korea.
Culture conditions for mycelial growth of Poria cocos
Woo-Sik Jo,Ju-Ri Park,So-Ra Oh,Min-Gu Kang,Woo-Hyun Kim,Seung-Chun Park 한국버섯학회 2016 한국버섯학회지 Vol.14 No.1
This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts.
Ju-Ri Woo,Doo-Ho Choi,Muhammed Taofiq Hamza,Kyung-Oh Doh,Chang-Yoon Lee,Yeon-Sik Choo,Sangman Lee,Jong-Guk Kim,Heeyoun Bunch,Young-Bae Seu 한국균학회 2022 Mycobiology Vol.50 No.5
Regulation of proper gene expression is important for cellular and organismal survival, main- tenance, and growth. Abnormal gene expression, even for a single critical gene, can thwart cellular integrity and normal physiology to cause diseases, aging, and death. Therefore, gene expression profiling serves as a powerful tool to understand the pathology of diseases and to cure them. In this study, the difference in gene expression in Flammulina velutipes was compared between the wild type (WT) mushroom and the mutant one with clogging phe- nomenon. Differentially expressed transcripts were screened to identify the candidate genes responsible for the mutant phenotype using the DNA microarray analysis. A total of 88 genes including 60 upregulated and 28 downregulated genes were validated using the real- time quantitative PCR analysis. In addition, proteomic differences between the WT and mutant mushroom were analyzed using two–dimensional gel electrophoresis and matrix- assisted laser desorption/ionization-time of flight (MALDI-TOF). Interestingly, the genes iden- tified by these genomic and proteomic analyses were involved in stress response, transla- tion, and energy/sugar metabolism, including HSP70, elongation factor 2, and pyruvate kinase. Together, our data suggest that the aberrant expression of these genes attributes to the mutant clogging phenotype. We propose that these genes can be targeted to foster normal growth in F. velutipes.
Ju Ri Lee,Seung Wan Suh,Ji Won Han,Seonjeong Byun,Soon Jai Kwon,Kyoung Hwan Lee,Kyung Phil Kwak,Bong Jo Kim,김신겸,김정란,Tae Hui Kim,Seung-Ho Ryu,Seok Woo Moon,Joon Hyuk Park,Dong Woo Lee,Jong Chul Youn,D 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.8
Objective We investigated the impact of depressed mood (dysphoria) and loss of interest or pleasure (anhedonia)on the risk of dementia in cognitively-normal elderly individuals. Methods This study included 2,685 cognitively-normal elderly individuals who completed the baseline and 4-year follow-up assessments of the Korean Longitudinal Study on Cognitive Aging and Dementia. We ascertained the presence of dysphoria and anhedonia using the Mini International Neuropsychiatric Inventory. We defined subjective cognitive decline as the presence of subjective cognitive complaints without objective cognitive impairments. We analyzed the association of dysphoria and anhedonia with the risk of cognitive disorders using multinomial logistic regression analysis adjusted for age, sex, education, Cumulative Illness Rating Scale score, Apolipoprotein E genotype, and neuropsychological test performance. Results During the 4-year follow-up period, anhedonia was associated with an approximately twofold higher risk of mild cognitive impairment (OR=2.09, 95% CI=1.20–3.64, p=0.008) and fivefold higher risk of dementia (OR=5.07, 95% CI=1.44–17.92, p=0.012) but was not associated with the risk of subjective cognitive decline. In contrast, dysphoria was associated with an approximately twofold higher risk of subjective cognitive decline (OR=2.06, 95% CI=1.33–3.19, p=0.001) and 1.7-fold higher risk of mild cognitive impairment (OR=1.75, 95% CI=1.00–3.05, p=0.048) but was not associated with the risk of dementia. Conclusion Anhedonia, but not dysphoria, is a risk factor of dementia in cognitively-normal elderly individuals.
느티만가닥버섯의 ITS (internal transcribed spacer) 영역의 2차구조 분석
우주리(Ju-Ri Woo),윤혁준(Hyeokjun Yoon),유영현(Young-Hyun You),이창윤(Chang-Yun Lee),공원식(Won-Sik Kong),김종국(Jong-Guk Kim) 한국생명과학회 2013 생명과학회지 Vol.23 No.10
본 연구에서는 H. marmoreus 3-10균주와 H. marmoreus 1-1균주의 ribosomal DNA (rDNA) cluster의 분석이 수행되었다. Small subunit (SSU)와 intergenic spacer 2 (IGS 2)는 부분적으로 염기서열이 결정되었고, internal transcribed spacer 1 (ITS 1), 5.8S, internal transcribed spacer 2 (ITS 2), large subunit (LSU), intergenic spacer 1 (IGS 1), 5S는 완전하게 염기서열이 결정 되었다. 팽이버섯 H. marmoreus 3-10균주와 H. marmoreus 1-1균주의 rDNA cluster는 총 7,049 bp로 결정되었다. SSU은 1,796 bp, ITS1은 229 bp, 5.8S은 153 bp, ITS2는 223 bp, LSU은 3,348 bp, IGS1은 390 bp, IGS2은 900 bp로 염기서열이 분석되었다. 결정된 rDNA cluster의 총 7,049 bp 중에서 17 bp가 다름이 확인되었고, 각각 SSU (2 bp), ITS (3 bp), LSU (9 bp), IGS (3 bp)에서 차이를 확인하였다. ITS regions의 2차 구조 결과 5개의 stem-loop가 있음이 드러났다. 흥미롭게도, 이들 stem-loop 사이에서 stem-loopⅤ에서 한 개의 상이한 염기가 다른 2차 구조를 나타냄을 확인하였다. The ribosomal DNA (rDNA) clusters of Hypsizygus marmoreus 3-10 and H. marmoreus 1-1 were analyzed in this study. The small subunit (SSU) and intergenic spacer 2 (IGS 2) was partially sequenced. The internal transcribed spacer 1 (ITS 1), 5.8S, internal transcribed spacer 2 (ITS 2), large subunit (LSU), intergenic spacer 1 (IGS 1), and 5S were completely sequenced. The rDNA clusters of H. marmoreus 3-10 and H. marmoreus 1-1 were 7,049 bp in length. The sequence of SSU rDNA, which corresponded to 18S rDNA, was 1,796 bp in length, and the sequence of LSU rDNA, which corresponded to 28S rDNA, was 3,348 bp in length. The ITS region that variable region and IGS region that nontranscribed spacer was 462 bp and 1,290 bp in length. The sequence of 5.8S rDNA and 5S rDNA was 153 bp and 43 bp in length, respectively. The 17 bp of the rDNA cluster in the H. marmoreus 3-10 strain was different to that in the H. marmoreus 1-1 strain, with 2 bp in the SSU, 3 bp in the ITS, 9 bp in the LSU, and 3 bp in the IGS. The analysis of the secondary structure revealed that the ITS regions of H. marmoreus 3-10 and H. marmoreus 1-1 have five stem-loop structures. Interestingly, among these structures, one different nucleotide sequence resulted in a different secondary structure in stem-loop V.
Changes in Allergenicity of Porcine Serum Albumin by Gamma Irradiation
Koth Bong Woo Ri Kim,So Young Lee,Eu Jin Song,Jin Gyu Park,Ju Woon Lee,Myung Woo Byun,Kyu Earn Kim,Dong Hyun Ahn 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3
Pork is an excellent source of essential nutrients such as protein. However, pork can trigger hypersensitivity and serum albumin of pork is known as major allergen. In this study, to evaluate the effect of gamma irradiation on the allergenicity of porcine serum albumin (PSA), PSA solution was irradiated at 3, 5, 7, 10, 15, and 20 kGy. The changes in the ability of PSA to bind IgG and patient`s serum caused by gamma irradiation were observed by ci-ELISA and immunoblotting. SDS-PAGE was used for measuring the conformational change of gamma-irradiated PSA. The ability of 3-kGy-irradiated PSA to bind p-IgG and patient`s serum was decreased to 30% and 15%, respectively. The binding ability showed no significant differences among all irradiated samples. SDS-PAGE showed that the irradiated PSA bands were degraded and aggregated. Immunoblotting of irradiated PSA revealed that IgG and patient`s serum were rarely recognized at 3 kGy. Therefore, gamma irradiation could be applied to less-allergenic pork products.