23 이차원 전기영동법과 immunobotting을 이용한 Helicobacter pylori strain 26695, J99, 51의 항원 분석

김어진 ( E. J. Kim ),이민혜 ( M. H. Lee ),전진수 ( J. S. Jun ),박지희 ( J. H. Park ),서지현 ( J. H. Seo ),임재영 ( J. Y. Lim ),박찬후 ( C. H. Park ),최명범 ( M. B. Choi ),우향옥 ( H. O. Woo ),윤희상,고경혁 ( K. H. Ko ),백승철 ( S. C. Ba 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

Features and Properties of $YBa_2$$Cu_3$$O_{7-x}$ Films Grown on SrTi$O_3$ by High Frequency PLD

Shi, D.Q.,Ko, R.K.,Song, K.J.,Chung, J.K.,Choi, S.J.,Park, Y.M.,Shin, K.C.,Yoo, S.I.,Park, C. The Korean Superconductivity Society 2003 Progress in superconductivity Vol.5 No.1

YBCO films were deposited with various thicknesses from 100nm to 1.6$\mu\textrm{m}$ on single crystal $SrTiO_3$ substrates by pulsed laser deposition (PLD). The effects of different deposition conditions, especially different deposition rates by means of changing the pulsed laser frequency up to 200Hz, on the J$_{c}$ value were studied. For YBCO film with the thickness of 200nm, the $J_{c}$ value of $2.1MA/\textrm{cm}^2$ has been achieved under the high deposition rate of 3.2nm/s (190nm/min). The $J_{c}$ can be maintained greater than $1M/\textrm{cm}^2$ with the thickness less than 1$\mu\textrm{m}$. The X-ray analysis was used to examine the texture, crystallization and surface quality. The SEM was employed to analyze the surface of YBCO, and it was shown the surface of YBCO film became rougher with increasing the thickness. There were many large singular outgrowths and networks of outgrowths on the surface of YBCO films which lowered the density of thick YBCO film. The outgrowth network was probably the a-axis YBCO corresponding to XRD $\theta$-2$\theta$scan and $\chi$-scan which were used to characterize a-axis orientation of YBCO film. The reason for J$_{c}$ declining with increasing the thickness was studied and discussed.sed.

초기성인의 생활습관과 당뇨에 대한 지식

소선이,마지은,고지윤,김은혜,박현화,서유진,서형은,유은미,이하늬,이혜인,정지연,현혜리,이자형,정덕유,강지숙 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

Purposes :The purposes of this study was to investigate the self-managing status according to the life styles and the diabetic knowledge of early adulthood. The subjects in this study were 240people, the early adult aged twenties to thirties living in the metropolitan area. Data were collected by using self-administered questionnaires that measure about the knowledge of diabetes; the dietetic behavior; the knowledge of diet; the exercise behavior; the knowledge of exercise. Descriptive statistics, t-test, ANOVA, and correlation were used for data analysis. Result :1. At the knowledge of diabetes degree based on the general character, the subjects who had experiences of diabetic education have higher degree of the diabetic knowledge. 2. At the knowledge of diabetes degree according to the life style, the subjects who have regular diet are appeared highly. Also the subjects who don't take much food of animal origin have higher degree. 3. At the knowledge of diet based on the life style, the subjects who don't keep three times meals a day are appeared highly. Also the subjects who have regular diet have higher degree. 4. At the knowledge of exercise according to the life style, the subjects who take extra nutritions 3-4 times a week are appeared highly. Although the subjects tend to know about dietary, exercise and health management, it showed that they actually don't perform what they know in their everyday of life. The reason for this could be the lack of perceived seriousness about diabetes mellitus. Thus as nursing professionals, we should provide them proper health education and support. Moreover, advanced research on the empowerment is warranted.

Plate assay에 의한 Lactobacillus casei YIP 9018과 Bifidobacterium spp.의 항암효과에 관한 연구

高時煥,朴相瑨,李明燮,白永振,朴芝賢,金昌漢 건국대학교 생명과학연구원 1994 생명과학지 Vol.1 No.-

발효유 제품의 제조에 사용되고 있는 Lactobacillus casei YIT 9018(LC9018)과 장내 유용균인 Bifidobacterium bifidum 그리고 Bifidobacterium OLS1, OLS2, OLS3 및 OLS4의 인체암에 대한 in vitro 항암효과를 알아보기 위하여 plate assay를 실시한 결과 LC 9018의 균체를 동결건조하여 100㎍/㎖의 농도로 암세포에 처리하였을 때 폐암새포인 CALU-3과 SKMES-1, 유방암세포 HS-578T, 임파암세포 RAJI 및 색소암세포 FARROW에 대하여 직접적인 강한 항압활성을 나타내었다. 한편 In vitro 항균활성 조사에서는 공시균주 모두 그람양성균인 Sarcina lutea에 미약한 항균활성을 나타내었다. This study was carried out for investigating antitumor activity against human tumor cell lines of Lactobacillus casei YIT 9018(LC9018) which is in use to fermented milk products and Bifidobacterium spp. which is beneficial intestinal bacteria. Antitumor effects were examined by in vitro soft agar plate assay. Lyophilized mycelia of LC 9018(100㎍/㎖) had direct strong antitumor activity against human tumor cell lines, CALU-3, SKMES-1(lung cancer), HS-578T(breast cancer), RAJI(lymphoma cancer) and FARROW(melanoma cancer), but Bifidobacterium spp. did not show antitumor activity at all. On the other hand, att tested strains in vitro showed very weak antibacterial activety against Sarcina lutea.

중년층의 비처방약품 오남용 실태 연구

고미현,김민주,김유진,김정호,박예진,유리호,이민영,이송연,조윤진,황선주 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48

Purpose: The purpose of this study was to grasp the status of abusing Over The Counter Drug (OTC Drug) among the middle-aged. Methods: The participants of this study consisted of 175 middle-aged people in Seoul and Gyeoggi-do. Data were collected using a questionnaire, and the study was conducted from September 10 to October 10, 2013. The data were analyzed by t-test, chi-square test, one-way ANOVA and Turkey. Results : As a result, 97 participants(55.4%) out of 175 participants of this study had taken over the counter drug within the past one year. Their average score of abusive behavior of OTC drug was 6.96±3.01 out of 16. There was significant abusive behavior of OTC drug relative to the difference in family monthly income.(F=3.455, p=0.20) and there was significant difference depending on the information source of the OTC Drug.(F=2.56, p=.044) Conclusions : In conclusion, nurses should educate middle-aged people so that they can use over the counter drug more safely and accurately based on these results. In addition, these results will be useful in further studies regarding middle-aged people’s drug abuse according to the preceding data.

Anisotropy of the coherence length from critical currents in the stoichiometric superconductor LiFeAs

Koń,czykowski, M.,van der Beek, C. J.,Tanatar, M. A.,Mosser, V.,Song, Yoo Jang,Kwon, Yong Seung,Prozorov, R. American Physical Society 2011 Physical review. B, Condensed matter and materials Vol.84 No.18

드로잉 금형에 있어서 비드의 영향

고관영,최원정,조재희,명노훈,이창수,최병용,허주환,최창권,장종훈,장윤철,박기용 울산과학대학 2001 연구논문집 Vol.28 No.1

본 연구는 자체 제작한 다이 측 비드가 설치 된 드로잉 금형을 이용하여 SUS 304, 6:4 황동 판재를 드로잉 가공하여 생산된 제품의 치수변형 특성을, 비드를 설치하지 않은 자유 드로잉 하여 얻은 제품과 비교하였다. SUS 304 판재의 경우, 비드를 설치하여 드로잉 한 경우는 늘어난 길이의 변화가 연속적으로 증가하고 있고, 비드를 설치하지 않은 경우는 마지막 부분에서 다시 회복상태를 보였다. 그 원인은 드로잉 비드로 인하여 적정량의 유입으로 일정 비율로 늘어난 것이라고 볼 수 있다. 6:4 황동 판재의 경우는 비드를 설치한 경우의 치수 변형값이 비드를 설치하지 않은 경우보다 안정함을 보여주었다. In this research, properties of SUS 304 and 6:4 brass products produced by die bead-drawing mold were compared with those of products drawed without bead. In the case of bead-SUS-plate, variation of incremental length progressively increases, while, in the case of no bead-SUS-plate, recovery appeared at the final-part. The reason of this difference is considered as proper insert of metal-plate on the basis of bead. Bead-6:4 brass-plate showed more stable data than no-bead die case although it did not show recovery in final-part.

만기파종에서 수확시 숙기가 사일리지용 옥수수의 사초수량과 품질에 미치는 영향

고한종,박형수,김수곤,김종덕,권찬호,김종관 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.2

옥수수를 호밀과 조합한 연간 2모작의 작부체계하에서는 옥수수의 파종시기가 5월 중순이후로 지연된다. 따라서 본 시험은 전작물이 호밀 때문에 옥수수의 파종시기가 5월 21일로 지연되었을 때 건물수량과 품질을 극대화 할 수 있는 적정 수확시기를 결정하기 위하여 수행하였다. 사일리지용 옥수수는 서울대학교 부속목장에서 5월 21일에 파종하고, 수확은 8개의 다른 숙기에서 수확하였다. 수확시 숙기는 수포형성기(출사후 16일), 유숙기(20일), 유숙후기(26일), 연호숙기(28일), 황숙초기(33일), 황숙중기(38일), 황숙후기(44일), 및 생리적성숙기(53일)로 하였다. 황숙초기에서 황숙후기의 전식물체 건물률은 29.0∼38.5%로 사일리지 생산에 가장 적합하였다. 건물 및 가소화영양소총량(TDN) 수량이 최대인 시기는 황숙중기로 각각 14,831 및 10,675㎏/ha 이었다. 숙기가 진행됨에 따라 전식물체의 ADF 함량은 35.4%에서 22.1%로 감소하였고 NDF 함량 또한 63.8%에서 46.0%로 감소하였다 (P<0.05) ADF 및 NDF 함량의 감소는 옥수수의 부위별 함량 변화와 관련이 높다. 즉 숙기가 진행됨에 따라 옥수수의 부위중 암이삭은 증가하고 경엽은 상대적으로 감소하였다. ADF 함량에 의해 계산된 비유정미에너지(NEL) 및 TDN은 경엽의 경우에는 황숙초기까지는 감소하고 그 이후에는 증가하였으나, 종실과 전식물체의 NEL 및 TDN은 숙기가 진행됨에 따라 유의적으로 증가하였다. In vitro 건물소화율은 숙기가 진행됨에 따라 경엽은 61.1%에서 49.7%로 감소한 반면 전식물체는 58.3%에서 65.7%로 증가하였다(P<0.05). 이상의 결과로 미루어 볼 때 만기파종시 최대건물수량과 품질을 가진 사일러지용 옥수수를 생산하기 위해서는 황숙중기(1/2 milkline) 에서 황숙후기(2/3 milkline) 사이에 수확하여야 한다. 그리고 이때 출사후일수는 38일에서 44일 사이였다. The corn (Zea mays L) planting data in a double-cropping system is delayed until mid-May due to delayed rye harvest on May. This experiment was conducted to determine the optimum harvesting time for high yield and the best quality of silage corn at late planting date after harvesting winter rye. Coms were planted on 21 May and harvested at eight different maturity stage at Seoul National University Experimental Livestock Farm, Suwon in 1997. Maturities were B (blister; 16 days after silking), M (milk; 20 days), LM (late milk; 24 days), SD (soft dough; 28 days), ED (early dent; 33 days), FD (full dent; 38 days), LD (late dent; 44 days) and PM (physiological maturity; 53 days) stages. The percentage of whole plant dry matter (DM) showed optimum range for silage making (29.0 to 38.5%) when corn plant was harvested at between ED and LD stages. Maximum whole plant DM (14,831 ㎏/ha) and total digestible nutrients (TDN) yields (10,675 ㎏/ha) reached at full dent stage. The percentage of whole plant acid detergent fiber (ADF) was decreased from 35.4 to 22.1%, and that of neutral detergent fiber (NDF) was also decreased from 63.8 to 46.0% as harvest stage progressed. These changes in chemical compositions were associated with changes in plant part composition. A progressive increase in total ear, and the decrease in stover portion in the plant were observed with advance in harvest stage. Calculated on net energy for lactation (NEL) and TDN values based on ADF percentage of stover plant decreased by ED stage and then increased by PM stage. But NEL and TDN values of ear and whole plant increased as harvest stage progressed. While in vitro dry matter digestibility of stover was decreased from 61.1 to 49.7%, whole plant was increased from 58.3 to 65.7% as maturity advanced (P<0.05). The results of this study indicate that corn can be harvested for silage at full(1/2 milkline) and late dent (2/3 milkline) stages for maximum yield and optimum quality at late planting. And days after silking at late planting was 38 and 44 days.

아미노산 첨가수준에 따른 산란계용 사료의 단백질절약 효과

고영두,하정기,곽종형,강대진,송우준,한인규 경상대학교 축산진흥연구소 1981 畜産振興硏究所報 Vol.8 No.1

자유드로잉 제품과 패드를 설치한 드로잉 제품의 특성

고관영,명노훈,하종성,이창수,최창권,최병용,허기영,김광수,김동수,장종훈,장윤철,김세환,오정민,하민정,박향자 울산과학대학 2000 연구논문집 Vol.27 No.1

본 연구는 자체 제작한 전체 패드가 부착된 드로잉 금형을 이용하여 SUS, 연강, AI, 6:4황동 판재를 드로잉 가공하여 생산된 제품의 특성을 자유드로잉 하여 얻은 제품의 특성과 비교 분석하였다. SUS 판재의 경우 제품에 발생하는 현상은 벽 주름, 구변주름, 수직 파단, 드로잉상처, 이어링이 발생하였으며 길이변화는 펀치어깨부분에서 가장 많이 늘어남을 볼 수 있었고 벽 주름 부분에 있어서 접혀진 부분은 상대적으로 적게 늘어남을 볼 수 있었다. 연강 판재는 SUS 판재에서 보여준 현상을 나타내었으나 SUS 판재에 비해 수직 파단은 적게 일어난 반면 이어링이 더 크게 일어났다. AI 판재의 경우 수직 파단은 일어나지 않았으나 플랜지 주름과 밑면터짐이 발생하였다. 6:4황동의 경우는 전체패드의 AI 판재 드로잉에서와 같이 밑면터짐과 플랜지 주름의 발생이 일어났다.