RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

Ethylenediaminetetraacetic acid (EDTA)항응고제에 의한 Mean Corpusculaar Volume(MCV)변화

홍승민,박영진,최하여,임진,정옥연,이호준,이호영,장숙진,문대수 조선의대 1996 The Medical Journal of Chosun University Vol.21 No.2

치환페놀류로부터 2-메틸벤조푸란 유도체의 합성

崔洪大,徐弼子,孫炳華 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

황원자가 α-탄소상의 양이온을 안정화시키는 성질을 가짐은 널리 보고되어있다. 이 α-thiocarbocations를 이용한 탄소-탄소결합형성반응에 관한 연구의 일환으로서, 저자동은 Scheme 1처럼 Friedel-Crafts반응 또는 Pummerer반응조건하에서 발생되어지는 황원자와 acyl기(nitrile기를 포함)에 인접한 탄소양이온(1-acyⅠ-1-thiocarbocations)과 방향족 화합물로부터 arylacctic acid와 arylacetone 및 arylacetonitrile의 간편한 합성법을 개발하였다. 또한 1-acyⅠ-1-thiocarbocations를 적용하여 phenylacetic acid의 모핵구조를 포함하는 소염제인 aryalkanoic acid의 제법을 보고하였다.

초저온 냉기를 이용한 한냉치료후 동통유발점압통역치의 변화

홍대종,최기섭,박시복,김영호 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.1

원위피판과 이개연골을 이용한 외이재건치험례

최광배,민대홍,윤진호,김승홍 大韓成形外科學會 1982 Archives of Plastic Surgery Vol.9 No.2

The auricle, diverging from the side of the head, consists of a delicate shell of elastric cartilage between the anterior and posterior of the thin skins. Thus, a radiacl chage in the outlook for the results of ear reconstruction has occured in recent years due to the delicate anatomical specificity. Inspite of the challenge of the many plastic surgeons, successful resconstruction of acquired defects may be extrimely difficult or impossible- especially, in patients who have suffered total traumatic loss of the auricle in which the local soft tissues are destroyed or deeply scarred. We experienced the case of total auricular loss with severe periauricular abrasion and dirty tattooing, and can obtained successful total ear reconstuction using buried cartilage and the distant flap.

2-Chloro-2-(methylthio)acetophenone을 이용한 2-페닐벤조[b]퓨란 유도체의 합성

崔洪大,徐弼子,孫炳華 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

It is well known that 2-arylbenzo[b]furan derivatives are occured in natural products such as Styrax obassia, Sophora tomentosa, and Krameria ramosissima. In the course of our search on the carbon-carbon bond formation utilizing 1-acyl-l-thiocarbocationic intermediates, we showed that the reaction of substituted phenols with α-chloro-α-(methylthio)acetone (1) in the presence of Lewis acid provided a convenient method for the 2-methylbenzo[b]furans (3) through the reductive desulfurization of the resulting products (2) (Scheme 1). The present paper describes the one-step synthesis for 2-phenylbenzo[b]furan derivatives from the reaction of phenol or cresol isomers with 2-chloro-2-(methylthio) acetophenone in the presence of zinc chloride. Many procedures have been reported for the preparation of 2-phenylbenzofuran ring. Among them, the most frequently employed method is based upon the cyclodehydration reaction of aryloxyacetone moiety with standard dehydrating reagents(H₂SO₄, ZnCl₂, POCl₃, KOH or PPA).

鉛暴露 作業者의 鉛中毒 早期發見을 위한 集團檢診法 改善方案에 관한 硏究

홍대용,김장락,정주화,최현림,윤형렬 慶尙大學校 1995 論文集 Vol.34 No.1

This study was carried out to evaluate the feasibility of current lead indicators used in screening lead who were sspeted to have been exposed to lead were selected as 'case'and 207 female workers from non-lead industry were selected for 'control'. The results obtained were as follows: 1. The mean values of blood lead for case and control were 31.4㎍/dl, 22.2㎍/dl,respectively. The difference observed in the mean concentration between case and control was statistically signifi-ant(P<0.01) 2. The mean values for blood ZPP by hematofluorometer for case and control were 31.3㎍/dl, 25.4㎍/dl,respectiely. And difference between two groups was statistically significant(P<0.01). 3. No statistically significant correlation was found between working duation and blood lead, blood ZPPlovel. 4. According to the simple correlation analysis,ZPPand urine lead were found to be statistically correlated with blood lead(P<0.01) 5. According to the discriminant analysis using blood lead as a dependent variable, it showed 89.1% predictability when using 40㎍/dl as a cutting value which is used as a screenig cutting value for industry workers health examination, while 81.8% for 30㎍/dl,77.54% for 20㎍/dl, respectively. 6. Lead related simptons which is used for screening the lead ntoxicated patients were used as a dependent variables in multiple regression analysis to find useful lead indicator. And significant independent variables were found to be ZPP, Age, and Hct. 7. Above results suggest that objective evalution of feasibbility of several lead indicators should be developed to correct the disutility of current lead screeng system in Korea.

견관절 주위근의 스트레칭후 동통유발점압통역치의 변화

홍대종,최기섭,박시복,이상건 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.6

땅두릅뿌리를 이용한 혼합음료가 당뇨 및 고혈압 환자의 혈압과 혈액 성상에 미치는 영향

최미숙,도대홍,최도점 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.2

충청북도 청주시 흥덕구에 거주하고 있는 24명의 독거 노인 (남자 10명, 여자 14명)으로 당뇨병 환자이고 경계성 고혈압 이상에 해당되며, 60세 이상인 노인을 대상으로 혈압과 혈청학적인 검사를 실시하여 얻은 결과는 다음과 같다. 1. 수축기 혈압은 3주에 1주보다 A군에서는 4.9% 상승하였고 B군은 0.3%가 증가되었으며 C군에서는 5.9%가 감소되었다. 각 군의 3주 평균은 A군 158.3±24.3, B군 149.1±19.2, C군 143.9±5.1로 C군, B군, A군 순으로 통계적으로 유의하게 낮은 것으로 나타났다. 이완기 혈압은 1주에 비해 3주에 A군에서는 3.7% 증가하였고 B군은 0.3% 감소하였으며 C군에서는 1.5% 증가하였다. 각 군의 3주 평균은 A군 93.8±11.3, B군 93.2±11.2, C군 86.9±6.2로 C군, B군, A군 순으로 낮게 나타났다. 이 결과에 의하면 두릅음료가 수축기와 이완기 혈압 조절에 기여하는 것으로 보인다. 2. 혈당은 3주에 1주보다 A군에서는 0.5%가 증가하였고 B와 C군에서는 각각 13.9%와 17.5%가 감소되어 두릅음료가 혈당저하에 효과적인 것으로 나타났다. 3. 중성지방은 1주에 비해 3주에 A군에서 11.6% 증가되었고 B군에서는 5.0% 감소하였으며 C군에서는 3.8%가 증가되었다. 총콜레스테롤은 1주보다 3주에 A군에서는 2.3% 증가되었고 B와 C군에서는 각각 8.7%와 14.5%가 감소하였다. HDL-콜레스테롤은 A와 B군에서는 각각 4.8%와 13.2%가 낮아졌고 C군에서는 19.6%가 증가되었으며 LDL-콜레스테롤은 1주보다 3주에 A군에서 1.9% 상승하였으나 B와 C군에서는 각각 8.3%와 21.1%가 저하되었다. 이 실험 결과에 의하면 두릅음료가 총 콜레스테롤과 LDL-콜레스테롤 저하와 HDL-콜레스테롤 증가에 효과적인 것으로 나타났고 중성지방의 조절에는 효과적이지 못한 것으로 나타났다. 4. GOT는 3주에 1주에 비해서 A군은 16.4% 감소하였고 B군은 14.9% 증가하였으며 C군은 14.7%가 저하되었다. GPT는 3주에 1주보다 A군 1.0%, B군 43.0%, C군은 43.0%의 증가를 나타내었다. 총 빌리루빈은 3주에 1주에 비해 A군에서는 변화가 거의 없었고, B군에선 25% 감소를 보였으며 C군은 50%가 증가되어 GOT, GPT 및 총 빌리루빈의 농도는 두릅음료에 의해 저하되지 않는 것으로 나타났다. 5. 총 단백질은 3주째 1주보다 A군은 13.6%, B군은 8.6%, C군은 11.8%가 증가되었고 알부민은 3주째 1주에 비해 A와 C군은 증가가 없었고, B군에서만 11.6% 감소하였으며 혈중요소질소는 1주보다 3주에 A와 B군은 각각 6.3%와 14%가 감소되었고 C군에서는 2.2%가 증가되었으며 요산은 3주에 1주보다 A와 C군에서 각각 20.4%와 11.1%가 감소하였고 B군에서만 14.3%가 증가하여 두릅음료의 효과가 총 단백질, 알부민, 혈중요소질소 및 요산에서는 뚜렷하게 나타나지 않았다. Studies were carried out to observe the influence of the Aralia continentalis Kitagawa root on blood pressure and blood constituents of the 24 hypertensive elderly (10 men and 14 women aged over 60). Total subjects were divided into three groups according to taking antihypertensive medications (A), antihypertensive medications and mixing beverages with the Aralia continentalis Kitagawa root (b) or mixing beverages with Aralia continentalis Kitagawa root (C). The systolic blood pressure of C group was significantly lower than that of A and B groups. The diastolic blood pressure of C group was lower than A and B groups. The blood glucose level of C group was a little lower than that of A and B groups. The level of TG increased more in A group than C group and decreased in B group. The levels of T-cholesterol and LDL-cholesterol lowered in B and C groups and HDL-cholesterol level was higher in C group. The GOT, GPT and T-bilirubin levels of C group were significantly higher than others. Mixing beverages with Aralia continentalis Kitagawa root did not affect on blood protein and non-protein nitrogens. In conclusion, mixing beverages with Aralia continentalis Kitagawa root had an effect on lowering blood pressure, blood glucose, T-cholesterol and LDL-cholesterol concentrations.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11