맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미 꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 ㎎ GAE/g ME, 2.34 ㎎ CE/g ME 및 6.23 ㎎ TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 ㎎ GAE/g SF, 5.44 ㎎ CE/g SF 및 13.38 ㎎ TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 44.72 ㎍ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 41.80 ㎍ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 ㎎ TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 ㎎ TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다. The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 ㎎ gallic acid equivalent (GAE)/g ME, 2.34 ㎎ catechin equivalent (CE)/g ME, 6.23 ㎎ tannic acid equivalent (TAE)/g ME, and 44.72 ㎍ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 ㎎ GAE/g solvent fraction (SF), 5.44 ㎎ CE/g SF, and 13.38 ㎎ TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and 41.80 ㎍ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 ㎎ Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 ㎎ TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

응급실에서 소아환자의 진정제로서 케타민(Ketamine)의 사용

서정필,박준석,황태식,장석준,김승호 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Ketamine use in emergency room has been increased. It has rapid onset of action and appropriate duration of action. It does not need endotracheal intubation and produces potent analgesia, sedation, and amnesia. The object of this study is to determine the safety and the degree of physician's satisfaction in relation to ketamine use. Materials and Methods: This is a prospective study using protocol. Pediatric trauma patients who need sedation were given IM ketamine(4 ㎎/㎏) and atropine(0.01 ㎎/㎏) in a same syringe. Monitoring of patients was done by EM residents and complications of ketamine use were recorded. Physician's satisfaction was also recorded after the procedure. Results: Intramuscular ketamine was administered 54 times, mainly for laceration repair. Physicians completed protocol for 51 of treated children. The median time for onset of sedation was 6.5±2.4 min, and duration of action was 32.4±10.8 min. Hypersalivation occurred in 9.8%(n=5); random movement 3.9%(n=2), emesis during procedure(n=1), emesis at home(n=1), and transient oxygen desaturation(n=1). All were quickly identified and treated without specific airway management and sequelae. Of 84% of physicians was satisfied with ketamine use for pediatric sedation. Conclusion: Intramuscular ketamine can be administered safely and satisfactorily in emergency room to facilitate pediatric procedures in conjunction with a defined protocol and appropriate monitoring.

鬱血性心不全症에 對한 臨床的 觀察

徐明洙,金吉洙,金佑泰,張錫源,金承源,宋昌燮 대한적십자사 1983 적십자병원지 Vol.10 No.2

폐실질내의 주변형 결절로 나타난 소세포성 폐암의 CT 소견

이태희,최요원,전석철,서흥석,함창곡,박충기 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.2

We evaluated CT findings of the small cell carninoma, which presented as a solitary pulmonary nodule at the periphery of the lung parenchyma. Of pathologically-proven 45 patients with small cell carcinoma, 10 patients was included in this study, who had a solitary, peripheral lung nodule at distal portion of the subsegmental bronchus. We retrospectively reviewed clinical data and CT findings including size, location, margin, enhancement pattern, lymph node enlargement, metastasis. Peripheral small cell carcinomas were 22.2%(10/45). All masses had well-defined margin and lobulated margin was seen in 7 patients. Mean diameter was 3.8cm (2.5-7.0cm). Enhancement pattern were homogeneous in 3 cases and inhomogeneous in seven. Calcification or air-bronchogram was not present, and focal cavitation was seen in one case. In five, only lung mass was present, and lung mass with lymph node enlargement was seen in one. distant metastasis without lymph node enlargement was noted on two patients and another two patients showed lymph node enlargement and distant metastasis. Peripheral small cell carcinoma is not rare(22.2%). Most frequent CT finding is a well-defined, lobulated mass with inhomogeneous enhancement, these findings would be helpful in differentiating small cell carcinoma from other neuroendocrine tumors when differentiataion is difficult cytologically.

CITIS 지원 기술정보관리 시스템의 설계

정석찬,한태창,서범수,조장혁,주경준 한국경영과학회 1998 한국경영과학회 학술대회논문집 Vol.- No.1

CITIS(Contractor Integrated Technical Information Service) is a contractor's developed service to provide electronic access and/or delivery of contractually required information. CITIS satisfies one of the major CALS objectives to furnish a single-entry point for authorized access to contractor-generated Contract Data Requirement List(CDRL) data. A critical requirement for the practical implementation of the CITIS concept is the ability to access multiple existing heterogeneous databases in a fashion transparent to the CITIS user. In this paper, we will propose a prototype system model for CITIS, and discuss the system architecture of proposed CITIS system as a global information management system for distributed and heterogeneous local information systems. Furthermore, we will discuss about the required server module and using-scenario of proposed CITIS system.

한국인 관상동맥의 변화양상에 대한 형태학적 연구

권태정,이상용,이한영,고영창,권기석,조재홍,김태공,김유훈,조갑래,서중석,이원태 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

소비자의 과일 브랜드 인지 수준과 개선방안

이병서,위태석 한국식품유통학회 2004 食品流通硏究 Vol.21 No.1

This study discusses the consumer's awareness level and some improvement strategies. Main results are summarized as follows : (1) Consumers' awareness level for fruit brand is estimated by surveys for 600 housewives residing in Seoul. Its result shows that awareness level is very low and these results are caused by quality management, product differentiation, and promotion. (2) Main strategies are discussed in quality condition and brand promotions and advertising, and large scale retailers' private brand. Especially, quality improvement is needed in strengthening sweetness and safety. And the box labelling management is important in raising consumers' awareness level. Finally, producers' countravailing strategies are required to keep producer's brand power relative to the private brand

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

일본의 도매시장법 개정과 시사점

위태석,이병서,황규석 한국식품유통학회 2004 食品流通硏究 Vol.21 No.1

For the last few years, the wholesale market system in Japan has been experiencing a lot of changes in the related sectors. The greatest changes in the environments surrounding the wholesale market were enlargement of the producers' cooperatives, increase of agricultural products marketed in the outside of the wholesale market system, and management aggravation of the related businessmen. These changes resulted in the revision of the Wholesale Market Law in 1999 to cope with the social needs successfully. Major purposes of the revision of the law would be summarized as follows : 1. To improve management ability of the related businessman 2. To improve dealing method in the Central Wholesale Market 3. To promote reorganization of the wholesale market

요석 환자에서 대사 이상의 평가

이석영,서정원,윤석중,이상철,김용태,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: 요석은 대사적 질환의 일종이라 할 수 있으며 이는 소변의 유체 역학의 장애나 혹은 배설되는 대사 산물의 생화학적 불균형으로 초래하게 된다. 따라서 본 연구에서는 24시간 요검사를 통하여 요석 환자들에서 요중 대사적 이상에 대해 알아보고자 하였다. 대상 및 방법: 연속적으로 내원한 재발성 요석 환자 151명(남자 114명, 여자 37명)을 대상으로 하였으며, 요석이 완전 치유된 후 최소 3개월이 지나고 24시간 소변을 채취하여 요량, 나트륨, 인, 요산, 칼슘, 마그네슘, 수산 및 구연산에 대하여 분석하였으며 대사 이상의 종류 및 빈도를 조사하였다. 결과: 요석 환자들에서 가장 호발하는 대사적 이상은 저구연산뇨증(47.1%)이었으며 다음으로 고나트륨뇨증(43.2%), 저소변량(30.5%), 고칼슘뇨증(23.5%), 고요산뇨증(22.1%), 고수산뇨증(10.7%), 그리고 저마그네슘뇨증(9.4%) 순이었다. 151명의 환자 중 적어도 한가지 이상의 대사 이상이 136명에서 관찰되었다. 결론: 요석 환자들에서 요중 대사 이상은 90.1%에서 관찰되었으며 저구연산뇨증과 고나트륨뇨증이 요석의 생성과 재발에 중요한 역할을 하는 것으로 조사되었다. 이러한 요중대사적 평가는 요석 환자의 진단 및 예방에 유용하게 이용될 수 있을 것으로 사료된다. Purpose: Urolithiasis is a metabolic disease which arises from disturbances of the physico-chemical balance or the hydrodynamic system of the urine. This study was to determine the metabolic abnormalities through the 24-hour urine examination in patients with urolithiasis. Materials and Methods: The 24-hour urine specimens of 151 RSF (114 men, 37 women) were analyzed for volume, sodium, phosphorus, uric acid, calcium, magnesium, oxalate, and citrate. The incidence and frequency of metabolic abnomalities in RSF were determined. Results : The most frequent metabolic abnormality in RSF was hypocitraturia (47.1% ), followed by hypernatriuria (43.2%), low urine volume (30.5% ), hypercalciuria (23.5%), hperuricosuria (22.1%), hyperoxaluria (10.7%), and hypomagnesiuria (9.4%). At least one metabolic abnormality was found in 136 out of 151 RSF. Conclusion : Our results showed that RSF had metabolic abnormalities were found up to 90.1% of them. Hypocitraturla and hypernatriuria could play an important role in the development and recurrence of urolithiasis. This data suggested that metabolic evaluation might be the useful tools for urolithiasis detection and prevention.