Endothelial Cells Isolated from the Bovine Corpus Luteum Synthesize Prostaglandin F<SUB>2α</SUB> Receptor

Sun?Yeong Gwon,Ki?Jong Rhee,Seunghyung Lee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.3

The corpus luteum is a transient endocrine gland essential for regulation of the ovarian cycle as well as for establishing and maintaining pregnancy. Prostaglandin F2α (PGF) initiates functional and structural regression of the corpus luteum and therefore is an important regulator of the estrous cycle. It is a matter of debate whether the endothelial cells of the bovine corpus luteum express PGFR, the cognate receptor for PGF. Therefore, the aim of this study was to assess the expression of PGFR in bovine endothelial cells. Endothelial cells were isolated from the bovine corpus luteum of the mid?luteal stage using magnetic beads and cultured in vitro. We demonstrate that this isolation procedure generates a pure culture of endothelial cells as confirmed by synthesis of Factor VIII and lack of expression of 3β?hydroxysteroid dehydrogenase. By RT?PCR, Western blot and immunofluorescence analyses, we further show that the cultured endothelial cells produced PGFR. This model system can be utilized to provide an experimental system to investigate the role of PGF on endothelial cells during the reproductive cycle.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

외음부의 기저세포암

김보영 ( Bo Young Kim ),황선정 ( Sun Jung Hwang ),박병준 ( Byung Joon Park ),이은실 ( Eun Sil Lee ),한호섭 ( Ho Suap Han ),김용욱 ( Yong Wook Kim ),김태응 ( Tae Eung Kim ),노덕영 ( Duck Yeong Ro ),이안희 ( An Hi Lee ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.9

Basal cell carcinoma (BCC) is the most common human malignant neoplasm, accounting for 75% of all non-melanoma skin cancer. The incidence of BCC is strongly correlated with sun exposure as well as older age. Therefore, the vast majority of BCCs is observed in elderly patients on the sun-exposed skin of the head and neck with a frequency of more than 80%. BCC is very rare on sun-protected skin such as the perianal and genital regions and other etiologic factors must be considered in these cases. Although the pathogenesis of vulvar BCC is unclear, early diagnosis is very important. Because BCC in these areas sometimes seems innocuous, it is recommended that a biopsy of all suspect lesions be performed. We report a woman with BCC of the vulva treated with wide local resection and reviews the literatures on this subject.

주방용품에 대한 소비자구매 실태조사

이종영,김선희 경북대학교 1992 논문집 Vol.24 No.-

Because of the progress of import liberalization and the rising labor cost, Korean kitchen ware are losing competitive power, like other industries in Korea. More and more the korean consumers are exposed to foreign products and various domestic brands which are competing indomestic kitchen were market. As a result, Korean firms face with more difficult situations. This situation requires Korean firms to adop different marketing strategies. It is necessary that the Korean ware firms adopt cosumer oriented marketing strategy. This study attempts to analize consumer behavior regarding kitchen ware. The main findings of research shows that consumer attitude is drastically changing. That is, there are clear difference between 20∼30 year old housewives and 40∼50 year old housewives in their attitude toward kitchen ware. ◁표 삽입▷ (원문을 참조하세요) The Younger consumers prefers kitchen ware made of stainless. And they prefer department stores, and other one-stop shoppings that have many items in one place. They acquire information from advertising and relatives. They are not so much concerned with high price and use import goods more than older generation. Accordingly, Marketing strategy of kitchen ware will have to decide which segment to target and focus on consumer oriented marketing in that target market.

인슐린 비의존성 당뇨병에 동반된 심상성 천포창 1 례

이상준,박진용,임태형,윤영호,권순대,남창욱,허규찬,박근용,구본식,김상표,장종억 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

저자들은 인슐린 비의존형 당뇨병 환자에서 통증성 구강내 미란 및 전신성 수포성 미란을 동반한 심상성 천포창 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Various skin conditions such as diabetic dermopathy, necrobiosis lipoidica diabeticorum, bullosis diabeticorum, granuloma annulare occur frequently in diabetes, although common lesions may be associated by chance. Pemphigus vulgaris(PV) is a blistering skin disease seen predominantly in elderly patients. This disorder is characterized by the loss of cohesion between epidermal cells with the resultant formation of intraepidermal blisters. These blisters rupture easily, leaving denuded areases that may crust and enlarge peripherally. In half or more of patients, lesions begin in the mouth: approximately 90% of patients have oromucosal involvement at some time during the course of their disease. But the relationship of this disorder with diabetes is not clear. We experienced a case of pemphigus vulgaris associated with NIDDM in 67 years old woman who was admitted due to painful oral ulcer and control of blood glucose, so we present this case with a review of literatures.

대호 간척기 토양의 염농도별 밭작물의 염해 평가

이승헌,류순호,설수일,안열,정영상,이상모 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts (ECe 1, 3, 9, 14, and 16 dS·m^(-1)) of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over 3 dS·m^(-1). Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were 14.2 dS·m^(-1) for radish, 11.4 dS·m^(-1) for Chinese cabbage, 10.2 dS·m^(-1) for tomato for red pepper, 8.9 dS·m^(-1) for buckwheat and green perilla, 8.6 dS·m^(-1) for soybean, and 8.9 dS·m^(-1) for tomato. At higher ECe that start the growth inhibition, increasing 1 dS·m^(-1) in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was 15.4~23.1 dS·m^(-1).

여행전자상거래와 eCRM에 관한 연구

이선희,양정영 문화관광연구학회 2002 문화관광연구 Vol.4 No.1

The main purpose of this study i find the criteria of analysis by systemizing the theory on EC and eCRM. The second is to make the hypothesis and the study model on the basis of EC and eCRM. The third is to give the strategic alternative for EC formation and eCRM of on-line travel agency on the basis of EC empirical analysis. The findings of research are fallow; First is that the factor of inducing travelers to visit continuously the website of on-line travel agency is not only the simple travel information, but addictive entertainment factor is also needed. The second, as the proposal to form the relationship between travelers and on-line travel agency, the analysis tool to catch definitely the traveler's need and wants is needed. When it is revealed that the factors of satisfaction and confidence on website of the on-line travel agency made an strong influence on on-line purchase intention and word-of-mouth behavior in Travel EC, it is known that the confidence of travel information provide and system is different from one of the existing travel agency.

장애인 입소시설과 특수학교의 구강건강관리 실태에 대한 조사 연구

이광희,김대업,하순영,박종석 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.2

전국의 123개 장애인 입소시설과 102개 특수학교를 대상으로 구강건강관리 실태를 파악하여 장애인의 구강건강관리 계획을 수립하는 데 사용할 수 있는 근거를 마련하고자 하였다. 설문지 조사법으로 연구하였다. 정기구강검진은 특수학교에서 연2회 실시하고 있었으나, 장애인 입소시설에서는 56.8%에서 시행하였다. 우식 예방 프로그램은 장애인 입소시설의 69.1%, 특수학교의 86.3%에서 시행하였으며 프로그램의 종류는 입소시설에서 규칙적 칫솔질이 58.1%, 특수학교에서 예방교육이 65.7%로 가장 많았다. 불소를 이용한 우식예방은 입소시설과 특수학교에서 각각 8.6%, 11.8%에서 시행하고 있었으며, 열구전색은 각각 6.8%, 6.9%에서 시행하였다. 치과치료를 시행하고 있는 입소시설과 특수학교에서는 각각 84.2%, 39.2%이었고, 입소시설에서 치료를 받기 위해 이용하는 곳은 치과의원(60.2%), 보건소(16.8%), 자원봉사자(15.3%), 대학병원(1.0%) 등이었다. 치료를 위해 치과의원에 방문하여 치료를 거부당한 경험이 있는 응답자는 17.7%이었다. 주요어 : 장애인, 입소시설, 특수학교, 정기구강검진, 우식예방, 치과치료. The purpose of study was to investigate the oral health care status in institutions and special schools for the disabled in Korea. Nurses in 123 institutions and nurse-teachers in 102 special schools were asked to fill questionnaires regarding periodic oral examination. preventive dental programs, and dental treatment. The results of this study were as follow. 1.56.8% of institutions implemented regular oral examinations and all of special schools did it twice a year. 2.Preventive programs for dental caries were done in 69.1% of the institutions and 86.3% of the special schools. Programs included regular toothbrushing and dental health education. 3.Dental treatment was done in 84.2% of the institutions and 39.2% of the special schools. Institutions utilized private dental clinics(60.2%), public health centers(16.8%), volunteers(15.3%), and dental hospitals. 4.17.7% of the institutions experienced the refusal to treat the disabled by private dental clinics.

2단 간헐폭기 시스템에서 aeration cycle이 질소 및 인 제거에 미치는 영향

정명선,이준호,서광범,김영관 江原大學校 附設 環境硏究所 2003 環境硏究 Vol.20 No.-

This bench-scale research investigated the aeration cycle(on/off) as the controlling factors for nitrogen and phosphorus removal in a 2-stage, intermittent aeration process. At this experiment, the aeration cycle time(air-on/air-offJ was 30min/30min, 60min/60min, 90min/90min. Organic matter removal was observed more than 90% regardless of the aeration cycle and phosphorus removal was relatively high when the aeration cycle time was 60min/GOmin. On the other hand, For all of the aeration cycle, TN removal was appeared less than 55%. This result was probably due to the limitation of the external substrate for heterotrophic nitrification and aerobic denitrification.

수면무호흡증의 다원수면기록 소견

김용조,이선우,왕성근 대한생물치료정신의학회 1996 생물치료정신의학 Vol.2 No.2

To identify the clinical characteristics of sleep apnea syndrome in Korean, author analyzed 24 cases of polysomnographic findings statistically, diagnosed as sleep apnea syndrome during May, 1995 to July,1996. The results were summarized as follows: 1. The mean age of subjects was 35.7 years, the distribution of age was 12 to 64 years, and male to female sex ratio was 5:1. 2. As for mean values of sleep parameters, total bed time was 429.8 minutes, waking after sleep onset was 93.4 minutes, total sleep time was 336.1 minutes and sleep efficiency was 77.7%. 3. The proportions of sleep stage 1, stage2,slow wave sleep, REM sleep, and movement time to total sleep time were 13.1%, 39.4%, 24.4%, 20.8%, and 2.2% respectively. 4. Mean AI(apnea index) was 28.7 and RD(respiration disturbance index ) was 34.3. 5. Waking after sleep onset correlated with total sleep time and sleep efficiency negatively, and with sleep stage 1 positively. Sleep efficiency correlated with sleep stage 1 negatively, and sleep stage 1 correlated with slow wave sleep negatively.