최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한국산 배의 Polyphenol 화합물군이 쥐의 면역기능에 미치는 영향

최희진,한호석,박정혜,배종호,우희섭,안봉전,배만종,김현구,최청 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from Korean pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. Especially polyphenol fraction II and III showed highly significant effect on Rosette forming cell, and allergy inhibition. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

영구형 항서맥 심박조율기 환자의 장기추적관찰

최현석,이무용,최영진,최성준,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우,이명용 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

신속한 간흡충증 진단과 혈청 및 혈구 변화양상 분석

박지현,최혜정,정석률 남서울대학교 2010 남서울대학교 논문집 Vol.16 No.2

Diagnosis of Clonorchis sinensis requires specific proteins as antigens and it has been developed by using a rapid detection kit which identify circulating antibodies. In this study during Namseoul University Health/Medicine Care Service conducted in October 2010, with the residents in Wondeok-eup Samcheok Kangwon-do, the rapid diagnosis of C. sinensis infections was performed and patients' blood sera were analyzed. From 12 patients' sera of total 83, antibodies to C. sinensis were detected. According to the patients' sera analysis, about 73% of Total bilirubin, 40% of Cholesterol and 40% of Creatinine were abnormally exhibited. In the C. sinensis-positive sera detected by a rapid diagnostic kit, about 92% of Total bilirubin, 33% of Cholesterol and 50% of Creatinine were abnormally exhibited. According to analysis of blood cells, there was no abnormal result except for Hematocrit. In particular, high Eosinophil which means high incidence of C. sinensis infection was not observed. Taken together, it implied that the positive diagnosis of clonorchiasis would be previous infections of C. sinensis and its patients recovered from clonorchiasis have still antibodies in their sera. In addition, it also suggested that C. sinensis-positivity by the rapid diagnostic kit be not consistent with the present infections of C. sinensis. Key Words : Clonorchis sinensis, Rapid diagnostic kit, Serum, Blood cell, Antibody

다발성 간종괴의 형태로 나타난 특발성 과호산구 증후군 치험1예

최승오,연규민,이승민,김순제,왕준호,박형석,이재동,진춘조,이창훈,신현준,김정은 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Hypereosinophilic syndrome is characterized by persistent blood eosinophilia of 1,500/ul or more in the absence of known causes and multiorgan dysfunction by eosinophil-related tissue damage. In Korea, some cases of hypereosinophilic syndrome with hepatic involvement have been described with prolonged benign clinical courses. Most of them were diffuse or multifocal lesions in imaging studies, and a few case presenting as a single mass has been described. Here we report a case of hypereosinophilic syndrome with hepatic involvement in a 65-year-old man who presented with multifocal mass lesions. By abdominal US and CT scan, multiple, ill-defined nodules were detected in throughout the liver. Liver biopsy revealed severe eosinophilic hepatitis, suggestive of hypereosinophilic syndrome. Hepatic mass lesions improved after steroid administration for several weeks.

결혼이주여성의 정보수준, 정보유용성, 정보욕구의 차이에 관한 연구

최송식,김현숙 부산대학교 한국민족문화연구소 2009 한국민족문화 Vol.34 No.-

본 논문은 결혼이주여성의 출신국, 결혼경로별로 정보수준, 정보유용성, 정보욕구가 어떻게 다른지를 밝히고자 하였다. 분석에 활용한 자료는 부산대학교 사회과학연구소가 조사한 「2008년 결혼이주여성의 이주전 정보연결망과 정보수준」이다. 이 자료에서 정보수준, 정보유용성, 정보욕구를 분석에 사용하였고, 분석방법은 t-test, ANOVA, 주성분분석을 하였다. 연구결과에 의하면, 인도네시아 출신의 여성이, 연애결혼을 한 여성의 배우자 정보수준이 가장 높았다. 그리고 정보유용성은 가족생활에서는 인도네시아 출신 여성이, 가족 및 친척 소개의 결혼경로가 가장 높았고, 사회생활에서는 필리핀 출신 여성이, 학교 및 직장동료 소개의 결혼경로가 가장 높았다. 정보욕구는 주성분 분석하여 기초생활, 가족관계, 사회생활로 나누었다. 기초생활욕구에서는 베트남 출신 여성과 학교/직장 동료 소개 결혼경로 집단이, 가족관계욕구에서는 필리핀 출신 여성과 연애를 통한 결혼경로 집단이, 사회생활욕구에서는 인도네시아 출신 여성과 연애를 통한 결혼경로 집단이 가장 높았다. 이를 바탕으로 본 마지막 결론 부분에서는 결혼이주여성의 정보수준과 정보욕구를 고려한 정보 지원체계의 방안을 제시한다. This study invested the difference of information levels of husband and Korea lives, information utility and information need according to their country, marriage path. Subjects were 251 marriage migration females lived Busan and GyeongNam. Data were analyzed by T-test, Anova, Principle component analysis. In study reveals, the information levels showed a higher level of females from Indonesia and cases of love marriage. Information utility had divided into family life and social life. Family life showed a higher level of females from Indonesia and cases of arranged marriage by their family and kinship. Social life showed a higher level of females from the Philippines, cases of arranged marriage by their school day's friends and co-workers. Factors among information need using principle component analysis had extracted into factors of fundamental life, of apprehension family relations and social life. Factors of fundamental life showed a higher need of females from Vietnam, Factors of apprehension of family relations showed a higher need of females from the Philippines and Factors of social life showed a higher need of females from Indonesia. The practical implication of these result were pointed out and discussed in the paper.

펜싱 종목별 연습 및 시합시 심박수 변화

최태석,이근배,천영호,이현수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This experiment was done on 30 fencers that had joined the national competition and had a research on theft heart rates during the fencing bout and got the following results. 1. In average, a male fencer have a height of 178.40㎝, weight of 72.50㎏, the height when sitting of 93.70㎝, the chest perimeter of 94.70㎝, arm length of 56.50㎝, and thigh parameter of 60.20㎝. In average, for a female fencer, had a height of 167.98㎝, weight of 59.00㎏, the height when sitting of 87.00㎝, chest perimeter of 84.50㎝, the thigh perimeter of 58.30㎝, and the arm length of 50.90㎝. From the result of testing a fencer's endurance by general footwork, it was proofed that the evaluation of respiratory function was needed more. 3. When comparing the heart rates of male fencers in the fencing bout of fleuret and epee, we found out that the fleuret fencer had a higher heart rate, in average of 17.06beat/min, than the epee fencer. However, there was not much difference with the heart rates during the bouts of sabre and fleuret Clearly, the bout during epee showed lowest heart rate among these three subjects. 4. The heart rates during female's fencing bout, there was not much difference with the heart rates in the male's fencing bout, and the heart rate during a bout of epee was about 18.59beat/min lower than fleuret.

AC8A-T6 알루미늄 合金材의 切削加工 特性에 關한 硏究

최현민,김경우,김우순,김용환,김동현,채왕석 한국공작기계학회 2002 한국공작기계학회 추계학술대회논문집 Vol.2002 No.-

In this study, examined the cutting characteristics of alumuminum alloy AC8A-T6 that is used to present car piston materials. And in been holding materials machining empirically escape as result that experiment comparison changing the cutting speed and feed on various condition to choose efficient machining condition. The following results can be summarized from this research. 1.As the cutting speed decreased, principal cutting force and thrust cutting force is increased, and reason that cutting force interacts greatly in the low cutting speed is thought by result by BUE's stabilization. 2.The feed speed and cutting speed increase, friction factor is decrescent and the cause appeared the thrust cutting force is fallen than cutting force relatively because chip flow according to increase of the feed rate is constraint. 3.Though specific cutting resistance grows cutting area and the feed rate are few, the cause was expose that shear angle decreases by rake face of tool gets into negative angle remarkably as wear of a cutting tool or defect part of workpiece is cut. 4.Cutting speed do greatly depth of cut is slow, surface roughness examined closely through an experiment that becomes bad, and know that it can get good surface that process cutting speed because do feed rate by 0.1㎜/rev low more than 250m/min to get good surface roughness can.