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Catalase 의 고정화를 이용한 알코올감지용 바이오센서의 특성 연구
김용환,유영제,주현,변석훈 한국화학공학회 1996 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.34 No.1
Catalase의 반응 특성을 이용하여 알코올 감지용 바이오센서를 제작하였으며, 알코올 계측 용도로의 적용 가능성을 살펴보았다. 일정농도의 과산화수소와 함께 측정하고자 하는 알코올을 센서의 반응부위에 동시 공급하여 catalase와의 경쟁 반응을 통하여 얻어진 과산화수소의 분해산물인 산소의 농도를 용존산소(DO) 전극을 통하여 측정하는 방법을 개발하였다. 제작된 센서는 에탄올뿐만 아니라 다른 알코올류에 대하여도 적용성이 있었고 센서의 측정범위는 18mM 정도며 30-90seconds 이내의 빠른 응답특성을 보여 주었다. 이는 기존의 NAD(H)를 동시에 공급해 주어야 하는 알코올 탈수소화효소 혹은 알코올 산화효소 등을 이용하는 계측시스템을 대신한 고속의 매우 단순화된 반응시스템으로서 기존의 알코올 계측 시스템을 대체할 수 있을 것으로 기대된다. An ethanol-sensing biosensor was developed using catalase reaction. The method consists of supplying alcohol and given concentration of hydrogen peroxide, which reacts competitively with catalase. The resulting amount of oxygen from this reaction is analysed using a dissolved oxygen(DO) electrode. The sensor can be also used for alcohols other than ethanol. In the developed sensor, the measurable concentration range was about 18 mM of ethanol and the response time was within 30-90 seconds. The proposed sensing system is simpler and better in sensing performance compared to the systems using alcohol oxidase or alcohol dehydrogenase where NAD(H) has to be supplied.
Evaluation on Clinical Application of Osmotic Pump with Dorzolamide in Normal Dogs
김용환,김주리,김민수 한국임상수의학회 2019 한국임상수의학회지 Vol.36 No.6
This study was designed to compare the effects of continuous release of an anti-glaucoma drug applied by an osmotic pump and by conventional eye drop instillation in normal beagle dogs, by measuring drug concentration in the blood and monitoring intra-ocular pressure (IOP). In group 1, an osmotic pump filled with Trusopt® was implanted subcutaneously over the right eye of each dog and the IOP was measured. In group 2, the right eye of each dog was administered with 2% dorzolamide (Trusopt®, Merck, USA) three times per day. Blood was sampled once per week in all groups. The IOP of the end of this study was 16.7 ± 0.58 mmHg in group 2 and 17.7 ± 2.52 mmHg in group 2. Dorzolamide concentration in blood increased continuously over 4 weeks up to 585.8 ± 79.42 μg/ L in group 1, and 747.3 ± 27.89 μg/L in group 2. We achieved satisfactory results in the osmotic pump group, which had a similar effect on IOP, and low fluctuations in IOP. Therefore, the results of this study should allow osmotic pumps to be consider as an alternative method to eye drops for the effective, safe, and convenient treatment of glaucoma.
김용환,손명환 고려대학교 공학기술연구소 1984 고려대학교 생산기술연구소 생기연논문집 Vol.20 No.1
It is generally very difficult that perform fine and good finish surfaces in turning because having many disadvantages in machining conditions as compared with that in boring machine. In the present study the variation of surface roughness in accordance with turning conditions was investigated by using the tapered boring bar with square cross section. As the results optimum turning conditions were obtained.