전기자극이 성견 골결손부에 매식된 임플란트 주위조직의 치유에 미치는 영향

심재창,김영준,김옥수,정현주 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.2

Several experimental studies showed that the application of small amounts of electric current to bone stimulated osteogenesis at the site of the cathode and suggested that electrical currents promote osseointegration around dental implants. The purpose of this study was to determine the effect of direct microcurrent to endosseous titanium implants placed in bone defects. The right and left 2nd, 3rd and 4th mandibular premolars in ten mongrel dogs (15kg of weight) were extracted. One month later, Ti-machined screw type implants(3.8 mm diameter×8.5 mm length, AVANA�, Ostem) were placed in surgically created circumferential defect area(width 5mm, depth 4mm). The implants were divided into three groups according to the treatment modalities: Control group- implants without electrical stimulation; Experimental group I- implants with allogenic demineralized freeze dried bone grafting and Experimental group Ⅱ- implants allogenic demineralized freeze dried bone grafting and electric stimulation. The animals were sacrificed in the 4th and 8th week after implant placement and un-decalcified specimens were prepared for histological and histometrical evaluation of bone-implant contact ratio (BIC) and bone formation area ratio (BFA) in defect area. Some specimens at 8 weeks after implantation were used for removal torque testing. Histologically, there were connective tissue infiltration in the coronal part of defect area in control and the experimental group I. whereas direct bone contact was found in the experimental group Ⅱ without connective tissue invasion. Average BIC ratios at 4 weeks of healing were 60.1% in the experimental group Ⅱ, 47.4% in the experimental group I and 42.7% in the control. Average BIC ratios at 8 weeks after implantation were 67.6% in the experimental group Ⅱ, 55.9% in the experimental group I and 54,6% in the control. The average BFA ratio was 84.0% in the experimental group Ⅱ, 71.8% in the experimental group I and 58.8% in the control at 4 weeks, and the BFA ratios were 89.6% in the experimental group Ⅱ, 81.4% in the experimental group I and 70.5% in the control at 8 weeks after implantation. The experimental group Ⅱ showed also significantly greater BIC and BFA ratios compared to the control and the experimental group I (p<0.05). The removal torque values at 8 weeks after implantation were 56 Ncm in the experimental group Ⅱ, 49 Ncm in the experimental group I and 43 Ncm in the control. There was a statistically significant difference among 3 groups(p<0.05) . These results suggest that electrical stimulation improve and accelerate bone healing around endosseous titanium implants in bone defect.

CBD으로 제작한 In:Cd_(1-x)Zn_(x)S/CdTe 태양전지의 전기적 특성에 관한 연구

심재선,송우창,방영근,김남석,임창민,장현 三陟大學校 2006 論文集 Vol.39 No.-

CdS thin films have been widely used a window material in heterojunction solar cells. In solar cell systems, where CdS thin films have been proved to be useful, partial substitution of Zn for Cd increases the optical window of the hetero-junction and also the diffusion potential. In this thesis, effects of the thickness of indium films and the annealing temperature on structural, optical and electrical properties of chemically deposited CdZnS films for the efficiency of the Cd_(1-x)Zn_(x)S/CdTe solar cells were investigated. Cd_(1-x)Zn_(x)S films were grown by chemical bath deposition (CBD), which is a very attractive method for low-cost and large-area solar cells. The open circuit voltage of Cd_(1-x)Zn_(x)S/CdTe solar cells increased due to reduced electron affinity difference between Cd_(1-x)Zn_(x)S and CdTe films. However, the conversion efficiency of Cd_(1-x)Zn_(x)S/CdTe solar cell decreased because of the higher series resistance(R_(s)) than CdS/CdTe solar cell. The series resistance and conversion efficiency of Cd_(1-x)Zn_(x)S/CdTe solar cell were improved by using indium-doped Cd_(1-x)Zn_(x)S films as window layer. Cd_(1-x)Zn_(x)S/CdTe solar cells fabricated with optimal conditions exhibited V_(oc)=630mV, J_(sc)=38.89mA/㎠ , FF=0.53, and η=13.2%

Tetracycline 처리된 차폐막이 치은 섬유아세포에 미치는 영향

심재창,김영준,정현주,박병주 전남대학교 치과대학 1999 전남치대논문집 Vol.11 No.1

The purpose of this study was to evaluate the effect of tetracycline-treated e-PTFE membrane on the human gingival fibroblast attachment. To evaluate the cell attachment to membrane, the number of gingival fibroblast attached to e-PTFE membrane was counted by hemocytometer. Also the cytotoxic effect of tetracycline-treated e-PTFE membrane on human gingival fibroblast was measured by Wataha's MTT assay. After tetracycline-treated barrier membrane was exposed to culture media for 24 hours, cell attaachment to membrane and cytotoxic effect were measured. The results were as follows; As for cell proliferation rate, total cell number increase in both control group and tetracycline-treated e-PTFE membrane in course of time, but the cell numbers were decreased to half in tetracycline-treated barrier membrane as in the control group. In MTT assay, tetracycline-treated e-PTFE membrane showed moderate to weak cytotoxic effect compared to non-treated control. At the begining, tetracycline-treated, so was barrier membrane released abundant tetracycline and so decreased the number of cell attached barrier membrane, but not almost observed cellular activity inhibition on attached cell and as decreasing tetracycline-treated barrier membrane, the number of attached cell were increased. These results might suggest that tetracycline-treated e-PTFE membrane showed relatively low proliferation rate of attached cells, but weak cytotoxic effect. So it is suggested tetracycline-treated e-PTFE membrane could bind gingival connective tissue normally and the healing site could gain stability.

‘융복합교육’ 기반 수업 설계 사례 연구 : 예비 교사를 중심으로

심수진,박재현,최수빈,안성호 이화여자대학교 교과교육연구소 2016 교과교육학연구 Vol.20 No.4

본 사례 연구는 융복합교육의 현장 실천과 적용가능성 및 교사의 융복합교육 전문성 제고에 기여하고자, 한 중학교 현장에서 교생실습의 일환으로 자생적으로 행해진 예비교사들의 융복합교육 기반 수업 설계 프로젝트를 분석한 것이다. 한 교사의 주도 하에 21명의 예비교사들이 다섯 개 모둠으로 나누어 협업함으로써 융복합수업을 설계하였는데, 본 논문에서는 그 교사의 서면 인터뷰와 예비교사들의 수업지도안, 발표, 전문가 컨설팅, 성찰지를 자료로 취합하여 정성적으로 분석하였다. 특히 융복합수업 지도안에 대해서는 이전에 체계화되어 있는 융복합교육 프로그램 구성 틀을 바탕으로 분석하였다. 연구 결과, 수업지도안은 각각 창의적으로 다양하게 융복합교육적 목표와 방식 및 맥락을 충분히 잘 반영하였으며 주제, 내용, 활동요소 등도 융복합에 부합하게 유기적으로 연결된 것이 발견되었다. 단, 자율적 실천 역량을 더욱 촉진하고 개인에서 세계로까지 맥락을 확대하며 이를 다채로운 활동들을 혼합하여 구성할 것이 요구되었다. 한편, 성찰지에 의하면, 이번 사례에 대해 예비교사들은 1) 융복합교육을 ‘과목 간 연계성’, ‘수업의 맥락화’, ‘학습자 중심’으로 차별화하였으며, 2) 융복합수업 설계를 하면서 얻은 ‘새로운 경험’을 통해 자기 과목에 대한 새로운 측면, 학생의 관점, 동료의 전문성, 그리고 협업을 가치 있는 것으로 성찰하였다. 3) 또한, 융복합교육의 절대적 필요성을 인식하고 교사 전문성 신장과 연구자 자세 함양을 되새기며 향후 융복합교육을 실천하겠다고 진술하였다. 이에 따라 본 연구는 현장의 교육 개선을 위해 교사의 재량권 보장과 행정적 지원이 필요함과 융복합교육과 관련하여 (예비)교사교육의 체계적이고 실증적인 개선이 필요함을 시사한다. To explore the implementability of, and the teacher expertise in “Yungbokhap education”, which embraces “consilient”, “convergent”, “integrative”, and “interdisciplinary” approaches to curricular integration, this case study analyzed a self-motivated project for pre-service teachers that was initiated as part of a practicum by a middle-school teacher who supervised the practicum. In the project, the pre-service teachers formed five groups and collaborated to create five integrative instructional unit plans based on Yungbokhap education. Their products positively impressed the school principal, mentor teachers, and other experts. They wrapped up their projects by answering reflective questions. This study analyzed their instructional unit designs and reflections qualitatively, in light of the instruction design framework of Yungbokhap education. The results show that the unit plans sufficiently reflect the goals, approaches and contexts of Yungbokhap education with appropriate creativity and variety, and tightly connect lesson themes, contents and activities, except for the fact that they could have further promoted the competency for autonomous action and expanded the context from an individual context to a local/global one employing more varied activities. According to their reflective responses, 1) they seem to characterize Yungbokhap education with features like “inter-subject connectivity”, “contextualization” and “learner-centeredness”, 2) their novel experiences in the lesson planning have led them to discover fresh values in their own subjects, students’ perspectives, peers’ expertises, and fruitful collaboration, and 3) their realization of the necessity of Yungbokhap education has positively challenged them to be more firmly oriented towards professional development and teacher-researcher attitude. These results imply that for autonomous and creative improvement of education, in-service teachers’ initiatives should be carefully respected and administratively supported, and that teacher preparation courses should provide pre-service teachers with more empirical chances to deal with curriculum integration.

뇌 동맥류 파열환자에서 안구운동 장애 : 증례 보고 Case Report

현용인,강재규,도종웅,정현태,심영기 최신의학사 1991 最新醫學 Vol.34 No.12

24시간 회상법으로 조사된 영양섭취 상태와 신체계측결과 비교분석 : 98 국민 건강·영양 조사

심재은,문현경 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.2

This analysis was performed to investigate the relationship between nutrition and anthropometric indices using the data from a cross-sectional survey of a large national sample, '98 Korean national health and nutrition examination survey. Subjects were selected by stratified multistage probability sampling design and completed dietary questionnares including food intakes for one day by 24-hour recall method. For this analysis, 6566 subjects were selected by age(over 20 years old). For anthropometry, height, weight, and waist- and hip- circumference were measured. They were classified by body mass index(BM1, weight(Kg)/height²m²)) and waist-hip ratio(WHR, waist circumference(cm)/hip circumference(cm)). The nutrients intake of subjects were compared with the recommended daily allowances(RDA). Mean adequacy ratio(MAR) was calculated. Mean heights, weights, BMIs were higher in the groups with nutrient intake over 125% of RDA than the lower intake groups for most nutrients. However, Mean WHR was the highest in the groups with nutrient intake under 75% of RDA for most nutrients excluding iron intake of women aged 20-64 years. Among women aged 20-64 years, means of MAR were 0.71 for obese individuals (BMI>30), 0.72 for subjects with underweight(BMI<18.5), and 0.76 for subjects with normal weight( 18.5 ≤BMK<2.5). Normal subjects has statistically significantly higher MAR than those of other groups. However, among elderly people aged over 65 years, obese group had the highest MAR, 0.68. Women with abdominal obesity(WHR> 0.9) had lower MAR, 0.71 than those with normal weight(MAR=0.76). From these results, obesity and abdominal obesity seems to be the results of malnutrition including both undernutrition and overnutrition rather than simple problem of excess energy intake. Obesity in elderly people needs to be handled differently from adults.

분산형 전원의 계통 연계에 따른 영향 및 시뮬레이션

심재선,박지웅,윤창대,임창민,장 현 三陟大學校 2006 論文集 Vol.39 No.-

As EG(Embedded Generation: photo- voltaic, wind, combined heat and power, fuel cell, small hydro etc.) grows fast in adopting to peak load reducing at the middle or the end of distribution system, there much has been interested in interconnection of EG. This paper discusses the various issue of a embedded generator to power system and shows the simulation of its various situation that could happen(focusing on load-flow by EG) by using a commercial software CYME(PSAF) for load-flow. With a result of above simulation, this paper shows a way of possible solution briefly.

제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

Developmental Characteristics of SCNT Pig Embryos Knocked-out of Alpha-1,3-Galactosyltransferase Gene

Joo-Hyun Shim1,Mi-Rung Park,Byoung-Chul Yang,Yeoung-Gyu Ko,Keon Bong Oh,Jeong-Woong Lee,Jae-Seok Woo,Eung-Woo Park,Soo-Bong Park,Seongsoo Hwang 한국동물번식학회 2009 Reproductive & developmental biology Vol.33 No.3

지역사회획득 폐렴의 치료지침 권고안

송재훈,정기석,강문원,김도진,배현주,서지영,심태선,안중현,안철민,우준희,이남용,이동건,이미숙,이상무,이영선,이혁민,정두련,지역사회획득 폐렴 치료지침 제정위원회 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

A successful therapy of community-acquired pneumonia requires appropriate empirical antimicrobial therapy. Etiology and antimicrobial susceptibility of major pathogens of pneumonia can differ by country. Therefore, an ideal treatment guideline of community-acquired pneumonia should be based on the studies performed in each country. We developed a treatment guideline for community-acquired pneumonia in immunocompetent adults in Korea. This guideline was developed by the joint committee of the Korean Society for Chemotherapy, the Korean Society of Infectious Diseases, and the Korean Academy of Tuberculosis and Respiratory diseases.