RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

반려동물의 기생충검사 이론 및 실제

이성호,박영석,박병권,김상기,정지윤,박수진,박창식,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The aim of this article is to improve human health and that of companion animals by improving the diagnosis and control of parasitic diseases. This is achieved by research into parasites of veterinary significance including zoonoses and the use of comparative systems to increase our understanding of some human parasitic diseases. We should promotes learning and teaching through knowledge and operates a diagnostic unit that offers a service to pet owner for indigenous and exotic parasitic diseases of companion animals. We must diagnose and treat them early. Zoonotic parasites are very important because many of animal parasites are zoonoses and give pain and death to human. Parasites are divided Protozoa, Helminthes and Arthropods. There are many protozoa, about 65,000 species. Arthropods are the vector of another diseases, such as virus, bacteria, protozoa and nematodes. Helminthes are composed of Trematoda, Cestoda, Nematoda and Acanthocephala. The target of Veterinary Parasitology is to recognize the characteristics of these parasites and subsequently diagnose, treat and control them. This article is intended to familiarize you with the essential facts and concepts of companion animal parasitology. Emphasis is placed on fecal sample techniques (comparison of fecal examination techniques; frequently found artifacts in fecal smears; direct Smear; zinc sulfate flotation; etc.) We recommend that the study be done to more references on animal parasites.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

온쓸개관을 묶은 집토끼 위날문점막의 미세구조 연구. : Ⅰ. 날문부 표면점액세포 Ⅰ. Pyloric Surface Mucous Cell

박경호,오승종,고정식,안의태,박대균,정은주 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The purpose of this study was to examine the ultrastructural characteristic of the normal pylorus mucosa, and their structural changes induced by the ligation of common bile duct of the rabbit. Common bile duct ligation probably causes the dysfunction of the pyloric surface mucous cells that results in delayed mucous formation and secretion, but recovered mucous secretory activity is recovered on the late stages.

UASB 반응기에서 제지폐수 처리를 위한 생물학적 속도론 모델에 관한 연구

박승조,이성식,전성균,김 광,신미옥,김미양,이영상 東亞大學校 大學院 1993 大學院論文集 Vol.18 No.-

The granulation of sludge in the UASB reactor is beneficial to stable operation of the reactor at a high volumetric COD loading rate and high hydraulic loading rate, because it improves the setting ability of sludge and the granular sludge acquired has high methanogenic activity. The design of integral biogas?/liquid/solids seperators varies among suppliers of UASB technology. Comples, soluble organic compounds are the first to be hydrolyzed (Stage 1) to simple organics. These simple organics are converted by acid-forming bacteria to C₄H?COOH, C₃H?COOH, CH₃COOH, H₂ and CO₂ in a acidogenic phase(STAGE 2). The higher organic acids are subsequently converted to CH₃COOH and H₂(Stag 3) by acetogenic bacteria. The final step(Stage 4) to produce CH₃ is carried out by me-thano-bacterium. The kinetics of anaerobic digestion of paper wastewaters largely depends on the environmental conditions. The purpose of this study was to elucidate the kinetics of methane production from paper was tewaters in the UASB. A four-step reaction mechanism was proposed for modeling methanogenesis of anaerobic treatment of paper waterwaters.

응급센터에 내원한 만성 폐쇄성 폐질환 환자에 대한 고찰

박승회,백광제,홍윤식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Chronic Obstrcitive Pulmonary Diseases(COPDs) produce significant morbvidity and mortality in human lives. COPD is a progressive disease that coexists with emphysema and chronic brochitis. The critical episodes depend on inciting or aggravating factors which can be classified into 3 ways. First, disease related aggravation. Second, Patient related aggravation. Third, Physician related(latrogenic). The authors reviewed 102 COPD patients who came to Korea University Hospital Emergency Medical center from March of 1992 to February of 1993. 1) Males were more frequently affected than females, and male to female ratio was 2.64 : 1 2) The average age of the patients were 62.8 years old. 3) The average number of hospital admission was 2.5 times and one patient was admitted 9 times for the same disease. 4) On an average, the patients had dyspneic symptoms for 15.6 years and the symptoms had developed since they were 47.8 years old. 5) The ratio between smokers and nonsmokers were3 2.1 : 1, the smokers had smoked for 36.3 years and the average amount of smoking was 1.5 pack per day. 6) After admission, sputum culture and sensitiveity test were done and the results were as follows : nonspceific organums 47 cases(46.0%), streptococcus 23 cases(22.5%), Pseudomonas 15 cases(14.7%), Hemophilus influenza 7 cases(6.8%), Klebsiella 5 cases(4.9%), Actinomycosis 3 cases (2.9%), Staphylococcus 2 cases(1.9%) 7) Aggravation factors for dyspnea were as follows : Upper Respiratory Tract Infection 40 cases(39.2%), Smoking 2.5 cases(24.5%), Pneumothorax 11 cases(10.8%), Pneumonia 7 cases(6.8%), Unknown origin 19 cases(18.6\%) 8) Prognosis after hospital admission were classified into 6 groups : 1. completely recovered 2. almost recovered 3. no improvement 4. hopeless discharge 5. expired within 48 hrs. 6. expired after 48hrs. The results were as follows : 87.3%(89 cases) discharged in almost recovered stated state, 1.9%(2 cases) discharged with no improvement, 4.9%(5 cases) were hoplessly discharged, 2.9%(3 cases) expired within 48 hrs, 2.9%(3 cases) expired after 48 hrs.

악성림프종 환자에서 복합화학요법으로 유발된 백혈구 감소증에 대한 인형 재조합 과립구 집락형성-촉진인자(rhG-CSF: Neutrogin)의 효과

박성규,전진우,기신영,유병우,김홍수,백승호,원종호,홍대식,박희숙 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Clinical effects of rhG-CSF were investigated in 17 patients receiving chemotherapy for non-Hodgkin's lymphoma. Patients were given G-CSF 2㎍/㎏/day after 2nd cycle chemotherapy for least 14 consecutive days, and clinical and laboratory results were compared to results of the first cycle. Treatment with G-CSF resulted in increase of mean WBC count(952.9±152.2㎜³vs 1882.4±350.4/㎜³) and neutrophil count(212.9±53.0/㎜³vs 841.5±238.0/㎜³), reduced the duration of leukocytopenic days(12.4±1.3 days vs 5.5±1.0 days, WBC count less than 4000/㎜³) and neutropenic days(4.5±0.9 days vs 2.3±0.7 days, neutrophil count less than 500/㎜³), and reduced the duration of neutropenic fever(5.8±1.1 days vs 0.9±0.5 days). The side effects of G-CSF were not so significant. These results demonstrated that G-CSF is safe and useful for the treatment of neutropenia induced by anticancer chemotherapy of non-Hodgkin's lymphoma and reducing the incidence of febrile periods and duration of antibiotic administration.

ATIS 최적 프로브차량대수 모형개발

박성식,고승영,서준석 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

To collect real-time link traffic information(Link Travel Time) for based on beacon and GPS technologies prove cars always have to be operate on the entire network. In this study, in case of taxi probe cars based with Beacon and GPS, method on determining required number of probe cars is studied to get traffic information of reality. Using taxis for probe cars, the the factors that influence the number of probe cars are operation time and distribution of prove cars over the network, operate distance and travel speed, the portion of revenue operation distance of taxis. The result of this study was ; As the collecting period for one traffic information prove cars travel more link, thus the number of prove cars decreases. The number of prove cars decreases when the allowable numer of links that no link travel times are collected increases.

Capsaicin 사전 투여에 의한 흰쥐의 공격성 감소 및 자율적 체온조절의 결손

박순권,홍승길,나흥식,김현택 한국심리학회 한국심리학회지 생물 및 생리 Vol.7 No.1

capsaicin 사전 처치가 흰쥐의 공격성과 체온조절에 미치는 영향을 알아보았다. 생후 6주경에 capsaicin을 피하주사하였고, 완전히 성숙한 후에 공격성 및 체온조절 기능을 검사하였다. 실험 1의 공격성 검사 결과 capsaicin 처치동물들의 공격성은 통제동물보다 낮았는데, 이것은 선행 연구의 결과와 상반된다. 체온조절 기능을 알아본 실험 2에서는 capsaicin 처치동물들이 37℃ 및 40℃ 조건에서 과체온과 빠른 체온증가를 보여주었다. 이것은 출생 직후 또는 성숙한 후에 약물을 투여한 선행연구들과 일치되는 결과이다. 따라서 capsaicin이 체온조절에 미치는 영향은 투여 시기와 무관한 것 같다. 논의에서는 본 연구의 두 가지 결과를 시상하부와 관련시켜 해석하였다. The present study was designed to examine effects of capsaicin administration on aggressive behaviors and autonomic thermoregulation in rats. In six-week-old rat, capsaicin was injected subcutaneously on 4 consecutive days in increasing doses(20㎎/㎏, 30㎎/㎏, 30㎎/㎏, 50㎎/㎏) to total of 150㎎/㎏ of the drug. The controls were treated in the same way with vehicle alone. Two experments began six or eight weeks after the treatment. In experiment 1, isolation-induced agressive behaviors, scored a 10-min session in the dyadic situation, were significantly decreased by capsaicin pretreatment. This result was not in accord with the previous findings. In experiment 2, body temperature of the capsaicin-treated rats increased more than the control's at two amibient temperatures studied(37℃ and 40℃). Our result concerning thermoregulation supports the preceding studies that applied to the capsaicin-treated animals as neonate or adult. Thus, it is likely that the effect of capsaicin treatment on thermoregulation has nothing to do with the age of capsaicin injection. The capsaicin effects from this study were compared with hypothalamic lesion effects in the discussion part.

유리纖維 補强 콘크리트 流動 特性에 관한 硏究

朴承範,任昌悳,尹義植 忠南大學校 産業技術硏究所 1989 산업기술연구논문집 Vol.4 No.1

Concrete is an excellent structural material, but it has many problems due to brittle behavior, weak resistance in tension, flexibility and toughness. In order to improve these problems, usually fiber reinforced concrete is used. But in the case of GFRC insufficient workability is caused by using glass fiber and there is lack of studying on it. This report of the following concrete of GFRC was experimented by the different W/C, S/A, glass fiber contents and maximum size of Aggregates. The results of this study is as follows; 1) Optimum S/A ratio is 0.6 at the glass fiber contents of from 0.25% to 0.75% in GFRC, but it cannot be obtained optimum S/A ratio at over 0.75. 2) Even though increasing the W/C ratio, we can get more slump, but slump is much more influenced by fiber contents in GFRC rather than increasing the W/C ratio. 3) Workability of GFRC is much more influenced by fiber contents than fiber length. 4) Workability of GFRC is a little improved by the bigger maximum size of aggregates.