DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

파트너십에 의한 지하철 입구 공간의 쌈지공원 설계 및 시공

김승환,강현우,손용욱,박승범,남정칠,이기철,차민준 東亞大學校 建設技術硏究所 2009 硏究論文集 Vol.32 No.2

A pocket park is valued in securing green space in downtown and improving urban view in the aspect of natural and environmental values, and it signifies a lot as the center of local residents’ community and harmony as well. Since subway exits and surroundings that have high portions in taking up street space due to lack of green space in downtown aren't made full use of, valuable pocket park place. Dangni Pocket Park of subway exits is a park completed with cooperative works and partnership by students, specialists, administrative organizations, businesses and civilian organizations, etc. without support of special budget. The study site was finally chosen to the space between exit No.1 of Dangni subway station, after analyzing the surrounding circumstances, availability and accessibility of the space among the several subway station exits of Saha, Dangni, and Hadan Station in Busan Metropolitan city. For the designing process, The Citizens organization for Million Amenity Park performed the planning and project proceeding, and 19 design drafts were proposed during the class of landscape architecture in Dong-a University. Design review panel members discussed to select the best draft in practical and authentical, and the related experts reviewed and revised to present the final design plan. The construction was conducted was performed under the cooperation between the civil, authorities, and industrial organizations, among which Saha District office managed for the waste treatment and maintenance matters. The graduates of landscape architecture department of Dong-a University and related industrial enterprises supplied landscape materials and related labors, were supported by 20 members of GPM, one of clubs of Dong-a University volunteered durig the most of construction progress, completing the Pocket Park of subway exits through partnership.

영화 관객의 온라인 평가 분석 -<박쥐>를 중심으로

박승현 ( Seung Hyun Park ),송현주 ( Hyun Joo Song ) 한국지역언론학연합회 2010 언론과학연구 Vol.10 No.4

전문적 영화평론가들의 평가는 영화의 흥행성과에 직접적인 영향을 미친다. 그러나 인터넷이 활성화되면서 영화평론가의 비평뿐만 아니라 일반인들이 온라인상에서 작성한 평가들의 중요성이 점차 증대되고 있다. 전문가 비평과 더불어 네티즌들의 영화평 또한 평가의 주요 요소로 등장하게 된 것이다. 이에 따라 최근 전통적인 요인들에 더해서 온라인 영화평이 영화의 흥행성과에 미치는 영향에 대한 연구들도 늘어나고 있다. 하지만 이러한 연구들은 가시적인 수치인 평점에만 주목하고 있을 뿐, 실제 네티즌 영화평이 포함하고 있는 내용이나 영화평의 전반적인 품질에 대해서는 등한시하고 있는 실정이다. 이 연구는 네티즌 영화평의 영향력이 점증하고 있는 상황에서, 탐색적 수준에서나마 네티즌 영화평의 내용과 품질을 분석하고 평가해 보고자 했다. 이를 위해 찬반 혹은 긍정/부정으로 양극화되어 활발하게 논의되었을 뿐만 아니라, 그 과정에서 감독과 배우의 스타 파워, 전문가 평가나 영화제 수상 경력이라는 요인이 영화의 흥행성과에 미칠 수 있는 영향을 상쇄했던 것으로 추정되는 영화 <박쥐>에 대한 네티즌 영화평을 분석대상으로 선정했다. 한 포털 사이트의 영화 게시판에 등록된 영화평 중에서 추천수와 영화평의 분량 등을 고려하여 총 191건을 표집한 후, 감독과 배우 평가, 감독의 전작 평가, 영화의 세부적 내용 평가 등을 분석 유목으로 삼아 내용 분석을 실시했고, 이후 영화평의 ``주장 목록``, 즉 얼마나 많은 주장이나 주장의 근거를 포함하고 있는가를 평가의 강도에 따라 비교 검토하여 하나의 의견으로서 영화평의 품질을 탐색적 차원에서 분석했다. 분석 결과 평가의 강도가 강했던 집단은 영화의 세부적 내용 평가나 영화에 대한 전체적 평가 모두에서 영화평의 주장 목록이 풍부했던 반면, 평가의 강도가 약했던 집단의 영화평의 주장 목록은 상대적으로 빈약한 것으로 나타났다. 결론에서는 영화평의 내용분석과 품질 평가가 영화 흥행성과 연구나 네티즌 영화평 연구에 대해 지니는 함의를 논의했다. Film critics, who provide the audience with basic information about a certain movie such as the genre, actors, the director, the plot, and the visual, create their own personal evaluations with positive or negative judgement on the quality of the film in question. Since those activities play a crucial role in audience`s decision on film selection, film critics are regarded as a significant factor to the box office performance. In these days, however, online reviews written by ordinary audiences are viewed as another important factor to the box office performance. Like critics, online reviewers discuss such aspects of the film as the cast, story, and visual images. They also make personal judgements with positive or negative evaluations on the film. As online reviews become meaningful either for the audience or for the box office result, we can sometimes witness critical difference of evaluation between audiences and critics. As revealed in Dragon Wars: D-War, released in 2007, netizens had quite a confrontation with critics who aroused negative opinions. It was considered a remarkable event, recognizing that online reviews by ordinary audiences became another significant factor which played a crucial role in people`s decision on film selection and furthermore in the evaluation of a film. The Dragon War event was followed by The Thirst in 2009, directed by Chan-Wook Park. The Thirst opened an itinerary away from the world in which critics are viewed as the unique influencer. This paper investigates online reviews on The Thirst in order to analyze the way how they discussed and evaluated it. By using a method of content analysis, this study analyzed the opinion quality of online reviews with such evaluation points as the director, the cast, the plot, the genre, and the visual, whose data were collected from the Naver Movies Web site. The valence of online reviews were mostly negative, even though there were opposing views between audiences. The results show that they were the most active in the opening week and became critical in that week, even though most critics were reluctant to present their negative perspectives. Content analysis also shows that online reviews were similar in discussion concerning the evaluation points with which critics usually dealt in their criticism. Online reviews are more critical of the story of film and the expressive qualities of violent cruelty. But they are very positive to the supporting actors. According to argument repertoire, there is a significant difference between a group of positive opinions and that of neutral opinions.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

The impact of blowflies on pig cadaver decomposition on Yeongdo Island, Busan, South Korea

Park Sang‐Hyun,Baek Seung‐Ho,Moon Tae‐Young 한국곤충학회 2021 Entomological Research Vol.51 No.11

The decomposition process is an important factor in forensics, and flies play a crucial role in the decomposition of animals and humans. However, there remains limited information on the effect of flies that attend death scenes. This study assesses the effect of the presence or absence of blowflies on the rate and process of cadaver decomposition in the spring, summer, and autumn from 2016 to 2020 on Yeongdo Island, Busan, South Korea. Nine blowfly species belonging to four genera in two subfamilies were sampled. The blowflies that approached the cadavers differed between seasons, but Chrysomya pinguis (Walker) was the dominant species regardless of the season. Blowfly presence on cadavers significantly affected the duration of the decomposition stage and the pattern of weight loss in nearly all scenarios. With the presence of blowflies, the decomposition rate was four times faster in spring, three times faster in summer, and nine times faster in autumn. These results show that the presence or absence of blowflies is an essential factor in the decomposition process of cadavers. Therefore, it should be taken into account when estimating postmortem intervals in medicolegal forensic entomology.

Cholelithiasis complicated with biliary sludge and urolithiasis in a dog

Seung-gon Lee,Dong-gun Kim,Joon-seok Lee,Ho-hyun Kwak,Hyun-sook Nam,Heung-myong Woo*, In-Chul Park**, Changbaig Hyun1,In-chul Park,Chang-baig Hyun 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3

A 10-year-old intact female Miniature Schnauzer dog was referred with the primary complaint of persistentanorexia, remittent fever, vomiting and abdominal pain. Hemogram suggested a chronic inflammatory disease. Serumbiochemistry showed moderate hepatobiliary cellular damage with severe cholestasis. Abdominal radiography andultrasonography revealed hepatomegaly, choleliths and sludges in gall bladder and small stones in urinary bladder. Basedcholecystectomy and cystectomy, choleliths and uroliths were removed from gall bladder and urinary bladder,respectively. The clinical condition was dramatically improved after surgery.

Rifampicin에 의한 것으로 추정되는 위막성 대장염1예

김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.

이면성 및 도플러 심초음파도를 이용하여 진단한 선천성 거대 좌회선지-좌심실 관동맥루

진승원,박준철,이영중,유병현,박현옥,김용주,윤호중,김재형,홍순조,최규보,권종범,원용순,박건 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

음향방출 파형 파라미터 필터링 기법을 이용한 실시간 음원 분류

조승현(Seung Hyun Cho),박재하(Jae Ha Park),안봉영(Bongyoung Ahn) 한국비파괴검사학회 2011 한국비파괴검사학회지 Vol.31 No.2

음향방출기법은 대형 구조물의 구조건전성감시(SHM)를 위한 매우 효율적인 방법이지만, 롤러코스터 지지구조물처럼 승용물의 운행으로 인한 매우 큰 잡음이 일상적으로 존재하는 경우에는 균열 진전 신호만을 분류하여 실시간 감시를 수행하기가 쉽지 않다. 이와 같은 문제의 해결을 위해 본 연구에서는 실시간으로 음원의 분류가 가능한 파형 파라미터 필터링 기법을 제안하였다. 파형 파라미터 필터링 기법은 음향방출 신호의 파형 파라미터를 이용하여 음향방출 히트를 사전에 필터링함으로써 실시간으로 감시하고자 하는 대상 음원만을 분류해내는데 매우 유리한 점이 있다. 다양한 음원에 대해 음향방출 파형 파라미터를 측정 및 분석하여 제안한 기법의 타당성을 살펴보았다. 또한, 파형 파라미터 필터가 내장된 음향 방출 시스템을 구축하고 이를 실제 롤러코스터 지지구조물에 적용하여 실시간 균열진전 감시를 위한 가능성을 타진하였다. The acoustic emission(AE) technique is a well established method to carry out structural health monitoring(SHM) of large structures. However, the real-time monitoring of the crack growth in the roller coaster support structures is not easy since the vehicle operation produces very large noise as well as crack growth. In this investigation, we present the waveform parameter filtering method to classify acoustic sources in real-time. This method filtrates only the AE hits by the target acoustic source as passing hits in a specific parameter band. According to various acoustic sources, the waveform parameters were measured and analyzed to verify the present filtering method. Also, the AE system employing the waveform parameter filter was manufactured and applied to the roller coaster support structure in an actual amusement park.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2