간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

추상 자료형 명세를 위한 객체 행동 분석지원 시스템 구현

송태섭,박석규 진주여자전문대학 1992 論文集 Vol.15 No.-

A large number of techniques have been devised, developed, and used for assistigng in the software development. Three such methods, representative of the entire set of such techniques, are Stepwise Refinement, Hierachical Decomposition, and Abstraction which is actively studied nowadays. All these three techniques greatly help solve the problems, while do not prevent the errors in the software product that was produced with these techniques. But abstraction technique supports object-oriented programming which creates more freely a representation of the real world porblem eomain and maps it into a solution that is software, it makes software development and maintenance easy. So these reason, object-oriented environment based on abstraction is currently being applied to software enginnering and data base. In this paper, after comparing and analyzing the previous abstract Data types implementation theory, we propose behavior specification method based on abstract Data Types, and implement Object Behavior Analysis Support System(OBASS) to output behavior specification to C++ classes.

심상성 루프스에서 Nested-Primer Gene Amplification Assay에 의한 결핵균 DNA의 검출

신문석,전효진,김병천,이규석,김재룡,권호준,송준영 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Lupus vulgaris, which is a progressive form of postprimary tuberculosis in patients with a moderate to high degree of immunity and tuberculin sensitivity, is the most common, most serious, and most variable type of cutaneous and mucous membrane tuberculosis. Domonstration of M.tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M.tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis. With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a nested PCR for detection of M. turberculosis. Compared with a single-step PCR, the two-step PCR was able to enhance sensitivity approximately 1,000-fold on the basis of bacterial counts and DNA quantity. We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M.tuberculosis in acid-fast stain negative specimens.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

漢江上流 人工湖에서의 微生物 分布와 活性度에 관하여

안태석,이동훈,김범철,조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

The values of total hacterial numbers, heterotrophic bacterial numbers, the proportions of specific enzyme releasing bacteria and microbial activities were measured in the five artificial reservoirs in North Han river. The values of total bacterial number ranged from 0.62x10E5cells/ml, which is the common values in mesotrophic lakes. With the effect of civil construction at the upper region of the North Han river, the proportions of alpha-glucosidase-releasing bacteria were higher in Lake Paro and Lake Chuncheon. Beta-glucosidase proportions were high in Lake Paldang and showed high correlation with the concentration of chlorophyll a. The alkaline phophlatase proportion were higher in Lake Paro, Lake Chuncheon and Lake Paldang. The turnover rate of acetate showed no relationship with any other trophic state index intems, such as chlorophyll concentration, phosphate concentration and primary productivity. But the phosphatase activity showed high correlation coefficience, and it should be estimated as a trophic state index in these artificial reservoirs.

소양호에서의 Alkaline Phosphatase 활성도의 Kinetics

안태석,김범철,조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

The kinetics of alkaline phosphatase activity were studied at the surface of a warm monomictic large reservoir, Lake Soyang, every two months from February 1988 to June 1989. Three sampling stations were located at the dam site, near a large netcage-type fishfarm, and a tributary stream inlet region where the dinoflagellate blooming occurs in warm seasons. Vmax of alkaling phosphatase, showing the range of 0.02∼5.95μ/M/l/hr, was high during the spring bloom, April and June, and low during the turnover time, October, December, and February.Kt + Sn, showing the range of 0.06∼31.0μ/M/l,was high during the turnoner time, which seems to be caused by the nutrients supply from the hypolimnion. Kt+Sn was much higher during turnover time at the fishfarm station than other stations, which is obviously resulting from the large amount of fecal deposition at the bottom and the diffusion of organic phosphorus upto the surface. Tt seems that at high density phytoplankton itself can be the substrate for alkaline phosphatase, since the peak time of Kt+Sn coincided with the peak of chlorophyll a concentration in the shallow stream inlet station where dinoflagellates aggregate.

Cluster analysis 에 의한 昭陽湖의 水體 分布

조규송,안태석,김범철,이동훈 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Cluster analysis was applied for the distinction of the water body in Soyang lake, which is the largest lake in Korea. For this analysis, the sixteen environmental parameters, such as water temperature, nutirent salts, organic materials, ets were used as the input data. The total data matrix was 16parameters X 47 points (in 14 sites). After Standardization, the Euclidean distances between two points were calculate. By the results, main stream of Soyang lake can be classified with 5 groups in March, 10 groups in February. And two shallow tributaries, showed different grouping pattern from main stream. In March, and November, the vertical mixing was detected, and in August, the water body above 10m was showed complex grouping pattern, and showed two water bodies in middle layer, and one in bottom layer.

金剛山댐 建設에 따른 北漢江 上流 水域의 環境 모니터링에 관한 硏究

조규송,김범철,안태석,전상호,이종범 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Aquatic ecosystem was studies in Lake Paro to monitor the effects of the Keumkangsan Dam construction in North Korea. Water quality, primary prodectivity, and microbes were serveyed bimonthly in 1987. The texture and composition of sediments, and meterological sapects were also investigated. The most remarkable change of water quality was the increase of turbidity. due to the dam construction. Overall chemical and biological aspects were greatly changed because of the high turbidity. The concentration of phosphate increased and subsequently phytoplankto standing corp increased. The effect of phosphate increase was compensated by the decrease of light penetration, and, therefore areal primary prodectivity was not so high as expected from the chlorophyll concentration. From the standing corp of microbes, the trophic state of lake Paro was evaluated as mesotrophy. As for the horizontal variation of microbial activity, the microbial heterotrophy was higher in the upstream regions than in the downstream regions. Sediment types are almost clayey silt and the mean sizes of sediments were homogeneous through the whole lake area. Organic carbon contents of the sediment seemed to be influenced by seasonal variation of primary productivity.

Factor analysis of the environmental impact on microbial populations in Soynag Lake

안태석,김철범,조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

The factor analysis was applied to scrutinize the effects of environental factors on bacterial popolation in Soyang lake. For this purpose total 294 cases of 18 physico-chemical and 2 bacterial parameters were observed. The major parts of the variance of the heterotrophic bacteria were interpreted as "inorganic nitrogen" and "depletion of dissolved oxygen", and theat of the psychrophilic bacteria was as "depletion of dissolved oxygen". But the "season in summer" and "organic substance" and "phytoplankton blooming" were minor factors for two bacterial population.

후궁성형술로 치료한 경추 척수병증 환자에서 방사선학적 소견이 예후에 미치는 영향

김석준,송준혁,김명현,박향권,김성학,신규만,박동빈 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.7