간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

직장 카르시노이드 종양에서 Ki-67 발현의 예후적 중요성

홍수민 ( Su Min Hong ),김유선 ( You Sun Kim ),문정섭 ( Jung Seop Moon ),김진남 ( Jin Nam Kim ),오명기 ( Myoung Ki Oh ),권선옥 ( Sun Ok Kwon ),정성연 ( Seong Yeon Jeong ),홍성우 ( Seong Woo Hong ),강윤경 ( Yun Kyung Kang ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Background/Aims: Rectal carcinoid tumors can be resected with endoscopy, and it is important to assess their prognostic factors. We evaluated the potential of Ki-67 expression as a prognostic factor in rectal carcinoid tumors. Methods: We retrospectively reviewed the medical records of 37 patients with rectal carcinoid tumors who got endoscopic resection from January 2001 to January 2011 at Inje University Seoul Paik Hospital. We analyzed their endoscopic and histologic findings, Ki-67 expression, clinical outcome, and prognosis. Results: The mean age (±SD) of the patients was 56.3±10.7 years, and the male : female ratio was 3.6:1. The mean tumor size was 0.5±0.4 cm, 33 patients showed grade 1 tumors (89.2%) and the average Ki-67 expression was 0.7±1.2%. Thirty five patients underwent endoscopic mucosal resection, and two required endoscopic submucosal dissection. Eight patients had positive margins after resection, but no cases of lymphovascular invasion were identified. The median follow-up duration was 21.4±25.4 months, and no recurrences were observed. Conclusions: In low grade rectal carcinoid tumors which are lack of central depression on colonoscopy, the expression of a molecular marker of malignant potential, Ki-67, was low. Therefore, endoscopic resection seemed to be a safe and effective treatment for these tumors.

실내 스모그 챔버의 설계 및 성능평가

배귀남,김민철,이승복,송기범,진현철,문길주 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

A multi-functional indoor smog chamber was designed and evaluated lo investigate photochenical or water vapor reaction mechanisms of air pollutants. Various smog chamber experiments could be conducted using ambient air or purified air in this smog chamber. The smog chamber consisted of a housing, a Teflon bag, blacklights, injection ports, sampling ports, and utility facilities. The characteristics of light source, the wall losses of air pollutants, and the quality of purified air were experimentally investigated. The maximum NO₂ photolysis rate was 1.10min^(-1). In a 2.5-m³ Teflon bag, the wall losses of ambient O₃, NO, and NO₂were 1.2~2.4×10^(-3)min^(-1), 0.7~2.0×10^(-3)min^(-1), and 0.4-2.0×10^(-3)min^(-1), respectively. The wall loss of ambient particles ranging 0.05 to 0.2㎛ was 1.8~5.4×10^(-3)min^(-1), which was slightly higher than those of ambient gaseous species. The purified air supply system provided high quality of air with NO₂, < 1 ppb, and total hydrocarbons <5 ppb.

유/무기 하이브라드 코팅액에 의한 냉간압연강판의 열처리 온도에 따른 내식특성

남기우(Ki Woo Nam),김정량(Jung Ryang Kim),최창민(Chang Min Choi) 한국해양공학회 2011 韓國海洋工學會誌 Vol.25 No.6

The demand for cold rolled steel (CR) for structural members is gradually increasing. If no surface treatment (coating for corrosion resistance) for CR is conducted, its use is very limited because CR is vulnerable to corrosion. Therifore, we need to develop a coating solution to provide high corrosion resistance for CR. In this study, an organic/inorganic coating solution with Si and Ti (Si polysilicate 7 wt. % + Urethane 13 wt. % + Ti amorphous 0.5 wt. %; LR-0727(1)) was used to evaluate the corrosian resistance of CR under a salt spray test. The specimens with the LR-0727(1) coating were heat treated in a drying oven at 120-210 ℃ for 5 min. The corrosion resistance was investigated using a salt spray test of 7 h. In addition, an adhesive test was conducted. Rust showed under a heat treatment of 150 ℃, but no vestiges were found over 160 ℃. The specimens with heat treatment at 160 ℃ or nwre did not experience delamination. From these results, it is considEred that the temperature limit for optimum heat treatment is 160 ℃ considering energy efficiency.

ISOLATION AND CHARACTERIZATION OF MOLECULAR MARKER FOR SEVERAL RACES OF PYRICULARIA ORYZAE USING RANDOM AMPLIFIED POLYMORPHIC DNA

Hong, Sun-Min,Bae, Dong-Won,Kim, Mee-Hyang,Shim, Chang-Ki,Kang, Kyu-Young,Kim, Nam-Soo,Kim, Hee-Kyu Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

Primer 155(GCTGGCGCTG) was the most useful primer with distinctive polymorphic DNA pattern to distinguish between KI races and KJ races. Primer 173(CAGGCGGCGT) showed polymorphic DNA pattern to distinguish isolates of KI-100 race. The specific DNA fragment of 0.6Kb, generated from 88-62 of KJ-201 race by RAPD with primer 412)TGCGCCGCTG), was promising as a molecular marker to identify the isolate.

Production of Iron-Binding Peptides from Colostral Whey by Enzymatic Hydrolysis

Kim, Sang-Bum,Ku, Min-Jung,Cho, Won-Mo,Ki, Kwang-Seok,Kim, Hyeon-Shup,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.6

Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at $100^{\circ}C$ Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at $50^{\circ}C$. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), ${\beta}$-lactoglobulin (${\beta}$-LG), and ${\alpha}$-lactalbumin (${\alpha}$-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not ${\beta}$-LG present in heated colostral whey. Alcalase completely eliminated BSA, ${\beta}$-LG, and ${\alpha}$-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.

Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

The relationship between aerobic capacity and the recruitment of activated sweat gland density with passive heating

( Tae Wook Kim ),( Jong Hyuck Kim ),( Nam Eun Bae ),( Hyung Seok Seo ),( Young Soo Baik ),( Jeong Beom Lee ),( Hun Mo Yang ),( Young Ki Min ) 한국운동영양학회 2012 Journal of exercise nutrition & biochemistry Vol.16 No.4

This study investigated whether the recruitment of activated sweat gland density during passive heating [immersion of the lower body into hot water, 41℃ for 30 min (PH), room temperature 23 ± 0.5℃ with 60 ± 3% relative humidity] is different in endurance-trained (Trainee), compared with that of untrained subjects (Control), as indicated by VO2max. Eight trainees and seven control female subjects (38.31 ± 4.91 ml·kg-1·min-1 and 31.8 ± 0.92 ml·kg-1·min-1, respectively, p < 0.01) were similar on all other physical characteristics. To compare the changes between the trainees and controls, serum cortisol and prolactin (PRL), tympanic temperature (TYMP), local activated sweat gland density (L-ASGD) and the mean whole body sweat loss volume (M-WBSLV) changes were measured after PH. The cortisol was significantly higher (p < 0.05) and the PRL tended to be higher in the Trainee than in the Control. The TYMP increased more in the Trainee than in the Control (p < 0.001). The L-ASGD showed a higher tendency in the Trainee than in the Control, there was a significant difference in the mean activated sweat gland density (p < 0.01) and the L-ASGD was significantly correlated with the VO2max (p < 0.001). The M-WBSLV was significantly higher in the Trainee (p < 0.001) and significantly correlated with the VO2max (p < 0.001). The results suggest that in humans, a high aerobic capacity is associated with a greater recruitment of activated sweat glands, whole body loss of volume and TYMP. Therefore, the decline in the heat loss responses, due to decrease in maximal oxygen consumption, may be masked by repeated endurance exercise training.

Production of Iron-Binding Peptides from Colostral Whey by Enzymatic Hydrolysis

Sang Bum Kim,Myoung Soo Nam,Kwang Seok Ki,Hyeon Shup Kim,Min Jung Ku,Won Mo Cho 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.6

Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at 100oC. Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at 50oC. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), β-lactoglobulin (β-LG), and α-lactalbumin (α-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not β-LG present in heated colostral whey. Alcalase completely eliminated BSA, β-LG, and α-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.

새마을運動의 地域社會組織事業的接近 : 地域社會組織事業의 社會計劃摸型을 中心으로

南基旻,曺興植 청주대학교 새마을연구소 1984 새마을硏究論文集 Vol.1 No.-

The achievements the Saemaul movement has made in the past ten years are successful to such a great extent that it is widely recognized both at home and abroad as the chief driving force for modernizing rural communities and national regeneration. The authors believe that with all its well-known success the Saemaul movement is still in the process of fulfilling its pledged ideals. Therefore, this requires a critical view of the strategies and technologies it has so far adopted in terms of its overall objective of inculcation of an improved work ethic among the Korean people. As an integrated and joint venture. to improve community situations, the Saemaul movement has to rely on the knowledge and skills emanated from various fields of professions. Modern professional social work, based on scientific knowledge and skill in human relations, concerns itself with organized activities aimed at helping individuals or communities to meet their basic needs, and at promoting their well-being in harmony with the interests of their families and communities. As a science and an art, social work could make a substantial contribution to the Saemaul movement. The authors have every reason to believe that our Saemaul movement with all its alleged success has somewhat neglected the utilization of professional social workers. Therefore, this paper attempts to identify the major problems the Saemaul movement has faced so far and to present feasible solutions to them by applying the community organization method with the schemes for problem-solving suggested by Robert Perlman and Arnold Gurin utilizing the social planning approach of Jack Rothman. There is no doubt that social workers, in general, and community organization workers, in particular have expertise in dealing with complex community problems, and can promote the Saemaul movement.