Properties of β-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Bae, Hyoung-Churl,Renchinkhand, Gereltuya,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius Nam27 with a high ${\beta}$-galactosidase activity was selected for enzymatic characterization. For purification, cell pellet was disrupted by Bead Beater, by DEAE-Sepharose and Mono-Q chromatography. The specific activity of the purified enzyme was 5,312 units/mg. The molecular weight of native monomeric ${\beta}$-galactosidase was estimated to be 30,000 dalton (monomer) by the SDS-PAGE. The optimum temperature and optimum pH were $50^{\circ}C$ and 5.0, respectively. This enzyme was stable between 35 and $55^{\circ}C$. ${\beta}$-galactosidase activity was lost rapidly above pH 7.0. But ${\beta}$-galactosidase was more stable at pH 4.0 (acidic conditions). And ${\beta}$-galactosidase activity was lost rapidly above $65^{\circ}C$ after 10 min incubation. $Ca^{2+}$ and $Zn^{2+}$ metal ions enhanced ${\beta}$-galactosidase activity by 164.09% and 127.37% while $Cu^{2+}$, $Fe^{3+}$ and $Mn^{2+}$ lowered ${\beta}$-galactosidase activity by 58.29%,85.10% and 77.66% respectively. Other metal ions didn't affect ${\beta}$-galactosidase activity significantly.

강성사면에 인접한 옹벽의 거동에 관한 연구

이명욱,유남재,박병수 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

This thesis is an experimental and numerical research on beating capacity acting retaining walls close to rigid slopes with stiff angles. Experiments were performed with changing the roughness of adjacent slope to the wall, its inclination, distance between wall and slope. Vertical stress and applied surcharge loads were measured by miniature earth cells and a load cel respectively. Stress distribution Vertical Settlement of surcharge of rigid model footing were measured by LVDTs. Bearing capacities of surcharge loads were compared with theoretical estimations by using several different methods of limit equilibrium and numerical analysis. For limit equilibrium methods, the modified silo and the wedge theories, proposed by Chung sung gyo and Chung in gyo(1994) were used to analyze test results Based on those modified theories, the particular solution with the boundary condition of surcharge loads on the surface of backfill was obtained to find the stress distributions acting in the backfill and to compare with test results. From results of surcharge test with model wall being very close to the slope, analyzed results by the modified silo theory and to be in the better agrements than other methods.

Properties of β-Galactosidase fromLactobacillus salivarius subsp. salivarius Nam27

Hyoung Churl Bae,Gereltuya Renchinkhand,Myoung Soo Nam 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius Nam27 with a high β-galactosidase activity was selected for enzymatic charac-The specific activity of the purified enzyme was 5,312 units/mg. The molecular weight of native monomeric β-galactosi-dase was estimated to be 30,000 dalton (monomer) by the SDS-PAGE. The optimum temperature and optimum pH were50°C and 5.0, respectively. This enzyme was stable between 35 and 55°C. β-Galactosidase activity was lost rapidly abovepH 7.0. But β-galactosidase was more stable at pH 4.0 (acidic conditions). And β-galactosidase activity was lost rapidlyabove 65°C after 10 min incubation. Ca2+ and Zn2+ metal ions enhanced βwhile Cu2+, Fe3+ and Mn2+ lowered β-galactosidase activity by 58.29%, 85.10% and 77.66%, respectively. Other metal ionsdidn’t affect β-galactosidase activity significantly.

오디첨가 요구르트의 발효특성

김혜경,배형철,남명수 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.1

오디의 건강 효과와 유산균 배양시 발효촉진 효과를 기대한 새로운 요구르트 개발의 일환으로, 환원 탈지유에 건조방법에 따른 오디의 첨가와 오디 첨가수준을 달리 한 요구르트 제조시 pH, 산도, 유산균수의 변화, 오디요구르트의 저장성 및 관능검사를 수행한 결과는 다음과 같다. 1. 생오디, 동결건조오디, 열풍건조오디는 보통 요구르트에 비하여 pH 저하와 산 생성의 증가가 빨라 발효촉진 효과를 보였으며, 처리구 간에는 동결건조오디나 열풍건조 오디에 비하여 생오디에서 그 효과가 두드러짐을 알 수 있었다. 2. 생오디의 첨가수준이 높을수록 pH 저하와 산생성의 증가가 빨라 발효촉진의 효과를 보였으며, 유산균수도 첨가수준이 높을수록 증가하였다. 3. 생오디 요구르트는 대조군에 비하여 유리당중의 fructose가 증가되었다. 4. 생오디 요구르트에서 lactic acid 함량은 대조군에 비하여 오디의 첨가량이 많을수록 더욱 증가되었다. 5. 생오디 요구르트의 관능검사 결과 외관(color), 맛(taste) 및 전체적인 기호도(overall acceptability)에서 유의적 차이를 보였으며, 향기(oder)와 조직감(texture)에서도 0.3%, 0.6% 처리구에 비하여 0.9%처리구가 좋은 결과를 보여 오디첨가요구르트의 제조 가능성을 보여주었다. 6. 생오디 요구르트의 저장실험 결과, 5℃에서 15일간 저장시 0.9% 오디첨가요구르트는 pH 4.04, 산도 1.082로 나타났고, 유산균수는 1.9×10^9cfu/ml로 우리나라의 호상 요구르트 총 유산균수 기준치 1.0×10^8cfu/ml를 초과하여 제품으로서의 우수성을 확인 할 수 있었다. This experiment was carried out to examine the fermentation properties of yogurt added fresh mulberry, freeze dried-mulberry and heated air dried-mulberry at concentrations of 0%, 0.3%, 0.6% and 0.9%. Lactic acid bacteria was used mixed starter culture(Str.salivarius ssp. thermophilus and Lac. delbrueckii ssp. bulgaricus). We obtained excellent results from the yogurt added fresh mulberry. In yogurt added 0.9% fresh-mulberry, lactic acid contents and titratable acidity were higher than those of 0%, 0.3%, 0.6% fresh-mulberry added. Also, sensory scores of color, taste and overall acceptability of the yogurt with 0.9% fresh mulberry was higher than those of any other groups.

Purification and Characterization of α-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Bae, Hyoung-Cchurl,Choi, Jong-Woo,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius CNU27 possessed a high level of ${\alpha}$-galactosidase activity. Purified ${\alpha}$-galactosidase was obtained after sonication of harvested cell pellet followed by DEAE-Sephadex A-50 and Mono Q anion exchange chromatography. The specific activity of the purified enzyme was 8,994 units/mg protein which is 17.09 times higher than that in crude extract. The native enzyme was a monomer with a molecular mass of 56,397.1 dalton. The optimum temperature and pH for the enzyme were $40^{\circ}C$ and 6.0, respectively. The enzyme was stable between 25 and $50^{\circ}C$. However, ${\alpha}$-galactosidase activity was lost rapidly below pH 4.5 and above pH 8.5. The enzyme activity decreased to 6.73% and 4.30% of the original activity by addition of $Cu^{2+}$ and $Hg^{2+}$, respectively. Other metal compounds did not affect the enzyme activity significantly. The enzyme liberated galactose from melibiose, raffinose, and stachyose. The rate of substrates hydrolysis was measured by HPLC. Raffinose, stachyose and melibiose were completely decomposed after 24 hr at $40^{\circ}C$.

임산부에서 발생한 폐크립토콕쿠스증을 동반한 크립토콕쿠스 수막뇌염 1예

박경화,송진수,최평균,방지환,조재현,박완범,김홍빈,김남중,오명돈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.4

Cryptoccus necoformans는 면역기능이 저하된 환자에서 기회 감염을 일으키는 진균이나 정상인에서도 드물게 질병을 일으킨다. 그러나 임산부에서 폐크립토콕쿠스증을 동반한 크립토콕쿠스 수막염의 보고는 아주 드물다. 저자들은 분만 후에도 악화되는 두통과 의식저하로 내원한 임신 37주의 임산부에서 뇌척수액과 폐조직검사를 통해 폐크립토콕쿠스증을 동반한 크립토콕쿠스 수막뇌염을 진단하고 항진균제를 사용하여 치료하였다. 본 증례처럼 명백한 기저질환이 없는 임산부에서도 크립토콕쿠스 수막염은 발생할 수 있으므로 의심이 되면 뇌척수액검사를 포함한 진단적 검사를 시행하고 적절한 치료를 시작해야한다. 그리고 임산부에서 크립토콕쿠스증이 발생한 경우의 치료에 대하여 문헌 고찰과 함께 보고하는 바이다. Cryptoccocosis occurs more frequently in immunocompromised hosts such as patients with AIDS, lymphoma, leukemia and other debilitating diseases. Pulmonary cryptococcosis can occur in the absence of any recognized immunodeficiency and cryptococcal meningitis can occur rarely during pregnancy. Although it is extremely rare, we encounter the reports of case of cryptococcal pneumonia and combined cryptococcal meningitis during pregnancy. We present here that 36-year-old pregnant woman, who was previously healthy, with cryptococcal meningoencephalitis and cryptococcal pneumonia was treated with amphotericin B and flucytosine. The maternal outcome was favorable, and there is no evidence of congenital infection in the newborn.

CORBA와 JAVA를 이용한 OBJECT WEB기반 주식매매관리시스템 설계

황준,김영신,송명남,조수영 서울여자대학교 자연과학연구소 1999 자연과학연구논문집 Vol.11 No.-

This paper proposes the infra-architecture that supports high scalability, performance, flexibility, security, conservation and officiency in designing the 3-tier stock market management systems using the Java and CORBA(Common Object Request Broker Architecture). This paper explains the OMG CORBA, Object Web and Java which is an object oriented interpreter language developed at Sun Microsystems. In this paper, we design the client user interfaces, application objects and database objects of the stock market management systems.

폴리에스테르 섬유 감량시스템용 프로세서 타이트레이터 개발

조진호,이흥락,이종현,김명남,구성모,김무영,진경찬,유병희,강민수,김국진 경북대학교 센서기술연구소 1993 연차보고서 Vol.1993 No.-

폴리에스테르 섬유 감량시스템의 제어를 위해서는 처리조 내에서 감량되는 섬유가 목표 감량치에 도달했는지를 판단해 주는 장치가 필요하다. 본 연구에서는 가성소다용액의 초농도 및 섬유 감량 정도의 정확한 측정과 감량 종료시간의 산출 및 이에 따른 종료신호를 발생할 수 있는 폴리에스테르 섬유 감량기 전용의 자동적정장치를 개발하였다. To control caustic soda treatment system for the polyester fabric reduction, we need the device to determine that the fabric has been arrived target reduction value. In this study, we develope an automatic process titrator for the caustic soda treatment system. Major functions of the target titrating system are accurate measurements of the caustic soda concentration and then producing the end point signal for caustic soda treatment system.

Purification and Characterization of α-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Hyoung Cchurl Bae,Jong Woo Choi,Myoung Soo Nam 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.1

Lactobacillus salivarius subsp. salivarius CNU27 posessed a high level of α-galactosidase activity. Purified α-galactosi-dase was obtained after sonication of harvested cell pellet followed by DEAE-Sephadex A-50 and Mono Q anion exchangechromatography. The specific activity of the purified enzyme was 8,994 units/mg protein which is 17.09 times higher thanthat in crude extract. The native enzyme was a monomer with a molecular mass of 56,397.1 dalton. The optimum tempera-ture and pH for the enzyme were 40oC and 6.0, respectively. The enzyme was stable between 25 and 50oC. However, α-galactosidase activity was lost rapidly below pH 4.5 and above pH 8.5. The enzyme activity decreased to 6.73% and 4.30%of the original activity by addition of Cu2+ and Hg2+significantly. The enzyme liberated galactose from melibiose, raffinose, and stachyose. The rate of substrates hydrolysiswas measured by HPLC. Raffinose, stachyose and melibiose were completely decomposed after 24 hr at 40oC.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2