분만 전·후 사료급여 형태가 젖소의 생산성에 미치는 영향

기광석,김현섭,이왕식,이현준,김상범,정하연,은정식,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This experiment was carried out to protect drops of feed intake should be plural prepartum and postpartum to reduce metabolic diseases after calving to know how feeding systems, which is divided as a low quility, a high quility and total mixed rations(TMR), affects on postpartum productivity of Holistein cows. Three diets (low or high quality forage separately fed with concentrate and TMR containing high quality roughage) were fed to 21 cows from 3 weeks prepartum to 8 weeks postpartum to examine their effects on the productivity of cows. DM intakes was noticed significantly higher with TMR (17.11kg/day) than low- quality (13.48 kg/day) and high-quality forage (13.10kg/day). TDN and CP intakes were also higher with TMR compared to other experimental diets. Mean daily milk yield was non-significant among the cows fed different diets. Blood non-esterified fatty acids(NEFA) content was higher in cows fed low-quality or high-quality forage separately with concentrate compared with those fed TMR. The results concluded that TMR feeding to transitional cows is better than feeding the low or high quality forage separately for their health and productivity. 본 시험은 분만 전 3주부터 분만 후 3주까지 전환기라고 지칭되는 시기의 젖소들에 대한 대사적 변화를 최소화하기 위한 방법으로 사료 섭취량 저하를 방지하고 분만 후 대사성 질병의 발생을 줄이기 위한 목적으로 수행되어졌다. 대사성 질병에 관여하는 주요 요인으로는 건물섭취량, 혈중 glucose 함량, NEFA 함량, 반추위 환경, 면역 등인데, 분만 전·후 Holstein 젖소에 사료급여 형태를 조·농분리(저질 조사료와 양질 조사료 급여구) 및 TMR로 다르게 하였을 때 사료섭취량 변화와 혈중 NEFA 및 glucose 함량 변화를 측정하고 분만 후 젖소의 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 처리별 사료건물 섭취량은 저질 조사료 조·농 분리, 양질조사료 조·농 분리, TMR 급여구에서 각각 13.48, 13.10, 17.11kg으로 TMR 급여구에서 유의성 있게 높았으며(P〈0.05), TDN 섭취량은 처리구별 각각 7.35, 7.19, 5.66kg으로 처리간에 차이가 없었다(P〉0.05). 신체충실지수(BCS)는 저질조사료구에서는 3.03이였으며, 양질 조사료구와 TMR 급여구에서는 각각 3.19, 3.16이었다. 분만후 1주부터 5주까지 측정한 평균 산유량은 저질조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 34.1, 35.1, 35.6kg 였으며, 분만 후 4∼5주에 최고 비유기에 달했다. 처리별 평균 유지율은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 3.74, 3.68, 3.95%였다. 혈청내 NEFA 함량은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 744.4, 381.8,266.7μEq/L로 처리간에 큰 차이를 보여 양질 조사료 급여와 TMR 급여시 혈중 NEFA 함량이 저질 조사료 급여에 이해 유의성 있게 낮았다(P〈0.05). 혈청내 glucose함량은 저질조사료 및 양질 조사료 조·농 분리, TMR 급여구에서 각각 39.3, 56.3, 62.4mg/dl로 양질 조사료 급여와 TMR 급여시 혈중 glucose 함량이 저질 조사료급여에 비해 유의성 있게 높았다(P〈0.05). 이상의 결과들을 종합해 볼 때, 분만 전 3주부터 분만 후 3주까지의 전환기 동안 사료섭취량 증가와 대사성 질병의 지표로서 혈중 NEFA 및glucose 함량 변화를 살펴 본 결과 분만 전 3주부터는 양질의 조사료나 TMR 형태로 급여하는 것이 사료섭취량 증가와 대사성 질병의 발생을 감소시킬 수 있을 것으로 사료되었다.

허브부식토의 사료내 첨가에 따른 In Vitro 발효특성과 젖소의 유생산성에 미치는 영향

김현섭(Hyeon Shup Kim),박중국(Joong Kook Park),김홍윤(Hong Yun Kim),김상범(Sang Bum Kim),양승학(Seung Hak Yang),김창현(Chang Hyun Kim),안종호(Jong Ho Ahn) 한국초지조사료학회 2011 한국초지조사료학회지 Vol.31 No.2

본 연구는 허브 부식토를 이용하여 첨가 수준별 in vitro 반추위 발효특성 평가와 젖소를 이용하여 급여시 유생산성에 미치는 영향을 조사하기 위하여 본 연구를 수행하였다. 시험 1에서는 티머시 건초를 기질로 하여 허브부식토(herbaceous peat)를 0, 1 및 5%를 3반복으로 각각 첨가하여 in vitro 반추위내 pH, 가스발생량, VFA (volatile fatty acid), ammonia-N 및 건물분해율을 조사하여 반추위내 발효성상의 변화를 평가하였다. pH 변화는 0, 3, 12 및 24시간 배양에 있어서 모든 처리구에서 유의한 차이를 보이지 않았지만 6시간, 대조구에서 1 및 5% 첨가구와 비교하여 유의하게 낮은 pH를 나타냈다 (p<0.05). 가스 발생량은 배양 12시간까지 처리구에서 대조구와 비교하여 유의하게 증가하였으며 (p<0.05), 반추위액내 암모니아 농도는 모든 처리구에서 24시간까지 증가하는 경향을 나타냈으며, 처리간 유의한 차이는 없었다. 건물분해율은 모든 시간에서 5%구가 대조구 및 1%구와 비교하여 건물분해율이 높았다 (p<0.05). 따라서 허브부식토를 0, 1 및 5% 수준으로 각각 첨가하여 반추위내 in vitro 배양시간별 반추위 발효특성은 대부분 조사항목에서 처리구가 대조구에 비해 개선효과를 나타냈으며, 시험 2에서는 젖소를 이용하여 사양시험을 통해 유생산성을 평가하였으며, 우유생산량 변화와 유성분 및 체세포 수 변화를 조사하기 위해 홀스타인 착유우 16두를 공시하여 4주간 사양시험을 실시하였다. 산유량은 T3구가 25.0 ㎏으로 대조구 (23.2 ㎏), 부식토 처리구 (23.1 ㎏), 비타민 C 처리구 (23.4 ㎏)와 비교하여 수치적으로 증가하였다. 대조구와 처리구별 시험시작전?후 유성분 (유지방, 유단백, 유당, MUN 및 SNF) 및 체세포 수의 변화는 일부 유의성이 인정되었다 (p<0.05). 4주간의 시험 기간 중 전반기 (1~2주)와 후반기 (3~4주)의 유성분 변화를 보면 부식토 첨가구에 있어서 유단백질과 SNF가 유의적으로 증가하는 경향을 나타내었다. 대조구와 처리구의 시험 시작 전?후의 혈액내 영양성분 (총단백질, 콜레스테롤, NEFA, BUN), 간기능 성분 (AST, GGT) 및 무기물 (Ca, P, Mg)에서 뚜렷한 변화를 나타내지 않았다. 이상의 결과를 종합해 볼 때 착유우에 부식토를 급여하는 것이 유성분, 체세포 수 및 생리적으로 긍정적인 영향을 줄 것으로 판단된다. This study was conducted to determine effects of dietary herbaceous peat on in vitro fermentation and milk production in dairy cows. Ruminal pH, gas production, VFA (volatile fatty acid), Ammonia-N, and rumen degradability were examined by the addition of three times over 0, 1, and 5% herbaceous peat with substrate of timothy hay, and the change of rumen fermentation characteristics were evaluated. In 0, 3, 12 and 24 hours cultivation, all treatments did not show a significant difference but the control at 6 hours appeared significantly lower pH compared to 1 and 5% treatments (p<0.05). The gas production of the treatments significantly increased until 12 hours of cultivation compared to control (p<0.05), the rumen ammonia concentration showed a tendency to increase until 24 hours in all treatment groups, and there was no significant difference between treatments. About the rumen degradability, 5% treatment showed higher rumen degradability in all hours than control and 1% treatment (p<0.05). Meanwhile, for in vivo trial, 16 heads of Holstein lactation dairy cows were selected for experiment for four weeks in order to research the change of milk yield, milk compositions and change of somatic cell counts of lactation dairy cows by herbaceous peat feeding. The milk yield of vitamin C and herbaceous peat treatments (T3) was 25.0 ㎏ but the control was 23.2 ㎏, herbaceous peat treatment (T1) was 23.1 ㎏, and vitamin C treatment (T2) was 23.4 ㎏, so there was linear increase effect of milk yield by T3. The partial significance of the milk (fat, milk protein, lactose, MUN and SNF) and change of somatic cell count before and after experiment by the control and treatments about change of milk and somatic cell counts (p<0.05) were recognized. About change of milk in the first half (1~2 weeks) and latter half (3~4 weeks) during four weeks of experiments period, the herbaceous peat supplement treatments showed a tendency of significant decrease of quality of milk protein and SNF. The control and treatments did not show significant change of blood nutrients (total protein, cholesterol, NEFA, BUN), liver function component (AST, GGT) and minerals (Ca, P, Mg) before and after experiment. In summary, it is judged that herbaceous peat feeding for lactation dairy cows would be recommendable based on the results of milk, somatic cell count physiologically.

번식률 향상을 위한 무인 발정발현 관찰시스템 구현

김석준(Kim, Suc-June),지선호(Jee, Sun-Ho),조현찬(Cho, Hyun-Chan),김춘수(Kim, Chun-Su),김현섭(Kim, Hyeon-Shup) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.9

본 논문에서는 무인 발정발현 관찰을 통해 다두 사육 시 발생하는 공태를 줄이고, 정확한 수정적기 판단을 통해 번식률을 향상하고자 하였다. 무리 생활을 하는 소의 특성상 운동량의 증가 유무만으로는 발정 발현의 판단이 어렵기 때문에, 소의 운동량 수집 센서와 개체 정보 종합 관리 프로그램을 개발하여 무인 발정발현 관찰시스템을 구축하고 운동량 정보와 번식 정보를 이용하여 발정 발현 관찰 알고리즘을 개발하였다. 시스템과 알고리즘에 대한 성능 검증은 수정 전 시스템을 통해 수정적기를 관찰하고, 수정하고 21일 후 재발정 여부를 확인, 다음 21일 후 초음파 진단을 통해 수태여부를 확인하여 적절하게 수정적기를 판단하였는지 검증하였고, 이를 규모가 유사한 실제 축산 농가 4곳에서 수행하였다. 각 농가의 총 사육 두수는 87, 81, 93, 82두이며 관찰된 발정 예정인 소는 14, 19, 15, 17두 이다. 이중 미약발정에 해당하는 3, 2, 1, 3두는 관찰에 실패하였으나 정상발정에 해당하는 11, 17, 14, 14두의 발정을 감지하였고, 인공 수정 후 10(91%), 17(100%), 13(93%), 14(100%)두의 소가 수태하여 제안된 무인 발정발현 관찰 시스템이 정상발정 시 수정적기 판단에 유효함을 보였다. In the paper, we reduce non-pregnant conditions and improve impregnation rate by unmanned estrus detection and decide proper time for artificial insemination. It is too hard to detect estrus only by using activities, we develop unmanned estrus detection system that consist of RF activity sensors, cow management program and estrus detection algorithm that uses information of activities and breeding. We verify performance by experiments in four similar scale stockbreeding farmhouse. Each stockbreeding farmhouse breeds 87, 81, 93, 82 cows and expected estrus cows are 14, 19, 15, 17. In expected estrus cow, we fail in weak estrus detection - 3, 2, 1, 3 cows, but detect successfully normal estrus - 11, 17, 14, 14 cows. After artificial insemination, 10, 17, 13, 14 cows became pregnant successfully confirming that proposed unmanned estrus detection system is effective for deciding proper time for artificial insemination in normal estrus.

소의 번식 및 발정 데이터 관리를 위한 소프트웨어 구현

김석준(Suc-June Kim),지선호(Sun-Ho Jee),조현찬(Hyun-Chan Cho),김춘수(Chun-Su Kim),김현섭(Hyeon-Shup Kim) 대한전기학회 2015 정보 및 제어 심포지엄 논문집 Vol.2015 No.10

애기수영 우점 산지초지에서 식생개선 기술적용이 식생구성 및 건물생산성에 미치는 영향

정종성,김종근,박형수,이상훈,김원호,김현섭,김영진,이효원,최기준,Jung, Jeong Sung,Kim, Jong Geun,Park, Hyung Soo,Lee, Sang Hoon,Kim, Hyeon Shup,Kim, Won Ho,Kim, Young Jin,Lee, Hyo won,Choi, Gi Jun 한국초지조사료학회 2016 한국초지조사료학회지 Vol.36 No.2

본 연구는 애기수영 우점 초지를 대상으로 종합적인 식생개선 기술을 적용하여 갱신한 초지의 연차별 식생 및 생산성 추이를 분석해보고 개량효과를 생태적 방법으로 고찰해 보고자 2013년 8월부터 2015년 10월까지 강원도 평창의 방목용 초지에서 수행되었다. 토양개량을 위해 석회를 $2MT\;ha^{-1}$의 양으로 2회 시용한 결과 pH 4.69에서 5.47까지 향상되었으며, 이후 더 이상 상승하지 않고 pH 4.91에서 4.98 수준을 유지하였다. 초장은 식생개선구가 모든 방목 시기에서 대조구보다 높은 경향을 보였으며, 방목 후 초장은 12~18 cm 사이로 대조구와 식생개선구간에 유의적 차이가 없었다. 초지 식생구성은 목초의 비율이 증가되었고 잡초(애기수영)의 비율은 12%에서 2%까지 감소하였다. 목초는 orchardgrass와 timothy의 비율이 높은 경향을 보였으며 시간이 지날수록 Kentucky bluegrass의 식생비율이 증가하였고 tall fescue의 비율은 감소하는 경향을 보였다. 방목두수는 한우 20두로 2014년도 총 방목기간은 58일이었고, 2015년도는 55일 이었다. 가축의 총 건물섭취량의 경우 2014년은 $7,848kg\;ha^{-1}$이었고 2015년에는 $6,941kg\;ha^{-1}$로 2014년도가 더 높은 경향을 보였다. 평균 초지 가축 이용률은 2014년도 84%로 2015년도 79% 보다 높은 경향을 보였다. 목초의 건물수량은 2014년도 1차 방목에서 대조구는 $3,437kg\;ha^{-1}$로 식생개선구 $5,460kg\;ha^{-1}$ 보다 생산량이 59% 증가되었고, 2015년도 총 건물수량에서 대조구는 $5,742kg\;ha^{-1}$로 식생개선구 $8,685kg\;ha^{-1}$ 보다 51% 건물수량이 증가되었다. This study was conducted to find out the effects of Improvement of Botanical Composition Technology (IBCT) on the forage production and ecological characteristics in a Rumex acetosella-dominated hilly pasture in Pyeongchang, in a period from August 2013 to the end of growing season in October 2015. $2MT\;ha^{-1}$ limestone was applied in August 2013 and April 2014. Liming significantly increased soil pH from 4.69 to 5.47 and then the soil pH consistently hovered at 4.91~4.98. For every grazing time, plant height was found to be higher with the application of IBCT as compared with the control. After grazing, no significant difference in plant height was found between the IBCT group and the control. Changes in the botanical composition of grassland can be affected by a lot of circumstances, but application of IBCT decreased Rumex acetosella from 12% to 2%, while also increasing the proportion of pasture. In relation to the botanical composition of grass, orchardgrass and timothy showed the highest percentages throughout the whole study period. The proportion of Kentucky bluegrass increased over time while tall fescue steadily decreased. The totals for grazing days were 58 in 2014 and 55 in 2015. Average herbage dry matter intake was $7,848kg\;ha^{-1}$ in 2014 and $6,941kg\;ha^{-1}$ in 2015. Due to the application of IBCT, dry matter productivity showed a significant increase of 59% during the first grazing period in 2014 (3,437 kg ha and 5,460 kg ha in control and IBCT). In 2015, the total dry matter yield of the IBCT group was 51% greater than the control ($5,742kg\;ha^{-1}$ and $8,685kg\;ha^{-1}$ in control and IBCT). Under acidic soil conditions, red sorrel can grow more strongly than grasses because it has better tolerance in dry and acidic conditions. Therefore, constant management is required in order to keep red sorrel from dominating the hilly pasture. In this study, the application of IBCT was able to effectively control weeds at all points. As a result, total management, including improvement of soil, overseeding, and application of selective herbicide and fertilizer, is needed to maintain excellent grassland conditions due to the limiting effects of environmental factors on grassland productivity.

Discharge properties of a lithium/sulfur cell at body temperature

Kim, Ic-Pyo,Park, Jin-Woo,Ahn, Hyo-Jun,Ahn, In-Shup,Kim, Ki-Won,Ahn, Jou-Hyeon,Ryu, Ho-Suk Royal Swedish Academy of Sciences 2010 Physica scripta Vol.2010 No.t139

<P>To investigate the feasibility of a Li/S cell for application to medical devices, the discharge properties of Li/S cells were investigated at 36.5 °C. A Li/S cell has the first discharge capacity 1158 mA h (g-S)<SUP>−1</SUP> at body temperature, whose cathode is composed of sulfur, carbon black and PVdF. The Li/S cells were discharged at various current densities to evaluate the rate capability, and the cycle performance at 100 mA g<SUP>−1</SUP> current density was also studied compared with previous reports.</P>

Production of Iron-Binding Peptides from Colostral Whey by Enzymatic Hydrolysis

Kim, Sang-Bum,Ku, Min-Jung,Cho, Won-Mo,Ki, Kwang-Seok,Kim, Hyeon-Shup,Nam, Myoung-Soo Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.6

Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at $100^{\circ}C$ Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at $50^{\circ}C$. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), ${\beta}$-lactoglobulin (${\beta}$-LG), and ${\alpha}$-lactalbumin (${\alpha}$-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not ${\beta}$-LG present in heated colostral whey. Alcalase completely eliminated BSA, ${\beta}$-LG, and ${\alpha}$-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.

Production of Iron-Binding Peptides from Colostral Whey by Enzymatic Hydrolysis

Sang Bum Kim,Myoung Soo Nam,Kwang Seok Ki,Hyeon Shup Kim,Min Jung Ku,Won Mo Cho 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.6

Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at 100oC. Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at 50oC. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), β-lactoglobulin (β-LG), and α-lactalbumin (α-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not β-LG present in heated colostral whey. Alcalase completely eliminated BSA, β-LG, and α-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.

Comparison of Forage Production and Nutritional Value of Italian ryegrass, Rye and Whole Crop Barley as Winter Forage Crops in Southern Region of Korea

So, Min Jeong,Kim, Hyeon Shup,Kim, Ji Hye,Lee, Sang-Hoon,Lee, Ki-Won,Jung, Jeong Sung,Sung, Kyung-Il,Peng, Jing lun,Park, Hyung Soo The Korean Society of Grassland and Forage Science 2015 한국초지조사료학회지 Vol.35 No.3

This study was conducted from October 2014 to May 2015 to explore forage production and feed values of Italian ryegrass, Rye and whole crop barley as winter forage crops in the Southern region of Korea. The experimental location was over 10 points for each species and each sampling point area was $1m^2$ (Width: 1 m ${\times}$ Length: 1 m). Air mean temperature and rainfall in the Southern region of Korea during the experimental period was $6.95{\pm}5.75^{\circ}C$ and $70.45{\pm}54.68mm$, respectively. Fresh forage yield of Italian ryegrass, the most cultivated forage in the Southern region of Korea, was $44.4{\pm}7.0ton/ha$. The percentage of dry matter for whole crop barley was $28.9{\pm}7.0%$. Crude protein (CP) was higher in Italian ryegrass ($10.7{\pm}5.3%$) while total digestible nutrient (TDN) had the highest value in whole crop barley. Crude protein was not significantly different by location. However, the neutral detergent fiber (NDF), acid detergent fiber (ADF) and total digestible nutrient value of forage from Jeonbuk province were higher than in forage from Gyeongnam province.

우유 및 유제품의 안전성 평가를 위한 미생물학적 위해요소의 위해평가

김현욱 ( Hyoun Wook Kim ),한기성 ( Gi Sung Han ),박범영 ( Beom Young Park ),정석근 ( Seok Geun Jeong ),김현섭 ( Hyeon Shup Kim ),오미화 ( Mi Hwa Oh ) 한국유가공기술과학회 2011 Journal of Dairy Science and Biotechnology (JMSB) Vol.29 No.2

Food borne pathogens are a growing concern for human health and food safety throughout the world. Milk and dairy products are commonly associated with spoilage or contamination from a wide variety of physical, microbial, and chemical hazardous. Microbiological risk analysis consists of three components: risk assessment, risk management, and risk communication, and overall objective of this process is ultimately public health protection. The microbiological risk assessment is useful tool to evaluate food safety as it is based on a scientific approach. In addition risk assesment process includes quantitative estimation of the probability of occurrence of microbial hazards to evaluate more accurate human exposure. The aim of this study is to review the microbiological risk assessment on the prevalence of bacterial foodborne pathogens in milk and dairy products.