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Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11
<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4
Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.
운동성 산화 스트레스와 항산화비타민의 보충이 말초단핵세포의 NF-kB 활성에 미치는 영향
진영수,박건구,박준영,김미정,이왕록,김혜영,이한준,박은경 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2
Reactive oxygen species(ROS) are implicated in the pathogenesis of a wide variety of human diseases. Numerous studies indicate that ROS may serve as common intracellular molecules that contribute to the process of nuclear factor(NF)κB activation in response to a diverse stimuli. In our laboratory, we have demonstrated tat antioxidants could reverse the decline of immune function caused by exercise-induced ROS. Furthermore, it in necessary to understand a mechanism underlying ROS-dependent disorder in biological system. Recent studies have been shown that several gene expression were regulated by oxidants, antioxidants and other determinants of the intracellular reduction-oxidation(redox) state. In this process, NF-κB have been shown to play a important role. The purpose of the present investigation was to evaluate the effect of exercise-induced oxidative stress and antioxidnt supplementation on NF-κB activation in peripheral mononuclear cells. Forty male SD rats(4 weeks old) were randomly divided into noraml diet group and antioxidants(ATO) supplement group, and then ATO groups were treated with antioxidants(VE: 2001U/kg, VC: 50mg/rat, β-carotene: 300mg/kg, vitamin B6: 250㎍/100g, selenomethionine: 0.1mg/kg) for 16 weeks. After 16 weeks breeding at each condition, each group divided into two groups: Control group(CR) fed generally, Exercise group(CE) fed generally and followed by acute exercise 16 weeks later. Antioxidant Antioxidants and Exercise group(AE) fed with antioxidants and followed by acute exercise. The activation of NF-κB binding activity was increased after exhaustive exercise bout in both group. In addition, pretreatment of ATO group with the antioxidants mixture lead to the inhibition of NF-κB binding activity. This results suggest that NF-κB activation should be further studies in response to a variety of exercise.
최진승,한미옥,김경원 동아대학교 교육대학원 2002 동아교육논총 Vol.28 No.-
This study research on service cognitions of college's students and their personality traits. The subjects are 1,417 college students (504 male students and 913 female students). The used tools are college student's personality test and questionnaires of college student's service activities. The sub-factors of personality traits are 12 traits as responsibility, leadership, diligent, independent, planning, tolerance, self-esteem, legality, generosity, anxiety, impulse, and masculinity. The categories of service activities on college students are 11 items as the importance of service activity to college students, the desire of service activity to college students, the beginning times of service activity to college students, the expense of service activity of college students, the times in one year of service activity to college students, the satisfactions of service activity to college students, the obstacles of service activity to college students, the obstacles of service activity to college students, the advantages of service activity to college students, the credit permissions of service activity to college students, the areas of service activity to collee students, the perspectives in future time of service activity to college students. From the results and discussions, conclusions as follows: 1.The college students on higher level traits as level traits as responsibility, leadership, diligent, planning, tolerance, self-esteem, legality, generosity, impulse, and masculinity recognize the importance of service activity. 2.The college students on higher level traits as responsibility, leadership, diligent, planning, tolerance, self-esteem, legality, and generosity recognize the importance of service activity. But the college students on higher level traits as independent recognize the unimportance of service activity. 3.The college students on higher level traits as leadership tolerance, self-esteem, and legality rrecognize the desire of service activity. 4.The college students on higher level traits as tolerance, legality, and generosity recognize the importance of self-expenses. 5.The college students on higher level traits as planning, tolerance, self-esteem, legality, generosity, and masculinity recognize the more than five times in a year as college students' service activity. 6.The college students on higher level traits as responsibility, leadership, diligent, independents planning, tolerance, self-esteem, legality, generosity, impulse, and masculinity recognize the satisfaction of service activity. 7.The college students on higher level traits as responsibility, leadership, planning, tolerance, and self-esteem recognize the obstacle as misunderstanding of college students, but the college students on higher level traits as independents recognize the obstacle as lake of systems and support from college and govemment. 8.There are no differences among the college student's traits and college student's advantages as spirits of sacrifice, professional knowledge, and a lot of times in college courses. 9.The college students on higher level traits as leadership, diligent, planning, tolerance, self-esteem, generosity, impulse, and masculinity recognize the importance of the credit permissions of service activity to college students. 10.The college students on higher level traits as responsibility and masculinity recognize the importance of visiting home for orphans, the college students on higher level traits as leadership recognize the importance of helping farm country, and the college students on higher level traits as independence recognize the importance of helping homelessness parents. 11.The college students on higher level traits as leadership, planning, tolerance, self-esteem, legality, and generosity recognize the positive perspectives in future time of service activity to college students.
박경미,정진홍 대전대학교 한방병원 2000 惠和醫學 Vol.9 No.2
In the Clinical Study on 1 case of Patient with U. T. I., the results were as follows. 1. U.T.I. is divided into pyelonephritis, cystitis, urethritis. In the range of Oriental Medicine, it belongs to Ryung(??), LimByung(淋病) and Ryobulli(尿不利). 2. The causes of U.T.I. are the descent of moist & heat by the yin-deficiency, the disturbance of vaporizing and the incurrence of temporary heat. 3. For this case patient, we can guess the cause as the descent of moist & heat at the thought of fever, costcovertebral pain and urinary pain. 4. For this patient, it was effective to dispense Paljungsan used for cystitis and urethritis induced by the descent of moist & heat. 5. Moxibustion was effective to cure this patient in company with herb-med and acupuncture.