탈진적 운동과 항산화제섭취가 산화적 손상과 항산화효소의 활성 및 항산화효소의 유전자 발현에 미치는 영향

이왕록 한국운동과학회 2003 운동과학 Vol.12 No.4

이왕록. 탈진적 운동과 항산화제섭취가 산화적 손상과 항산화효소의 활성 및 항산화효소의 유전자 발현에 미치는 영향. 운동과학, 제12권 제4호, 679-692, 2003. 본 연구의 목적은 최대 운동이 쥐 심장근 및 골격근의 산화적 손상정도와 항산화 효소의 활성에 미치는 영향을 규명하고 장기간의 복합 항산화제 섭취(비타민 E, C, B6, β-carotene, Selenium)가 최대 운동 시 쥐 심장근 및 골격근의 산화적 손상과 항산화효소의 활성에 미치는 영향을 규명하는 것이다. 본 연구의 실험동물은 태어난 지 4주가 된 Sprague-Dawley계 수컷 40마리를 대상으로 하였다. 실험동물은 무작위로 비항산화 섭취군(Non-antioxidants Group;CON, NAE)과 항산화 섭취군(Antioxidants Group; AC, AE)으로 나누어 실험을 수행하였다. 비항산화 섭취군(n=20)은 16주 동안 일반 쥐 사료를 섭취하였으며 항산화섭취군(n=2)은 일반쥐 사료와 함께 비타민E(200 IU/kg/day), 비타민 C(50 mg/rat/day), 비타민 B6(250 ug/kg/day), 베타카로틴(300 mg/kg/day)과 셀레니움(0.1 mg/kg/day)을 혼합한 사료를 같은 기간 섭취하였다. 식이 처치가 끝난 16주 뒤 운동군(n=20, NAE & AE)은 쥐 트레드밀을 이용하여 최대운동(25 m/min, 경사도 15%, ∼50±10 min)을 실시하였다. 심장근과 골격근에서 산화적 손상정도로써는 MDA(Melondialdehyde) 생성량을 항산화 효소로써는 SOD(Superoxide dismutase), GPx(Glutathione Peroxidase) 및 GR (Glutathione Reductase)의 활성을 측정하였고 항산화 유전자 발현량에 있어서는 Cu/Zn-SOD mRNA와 Mn-SOD mRNA의 발현량을 RT-PCR(Reverse Transcription - Polymerase Chain Reaciton) 방법으로 실험하였다. 실험결과 탈진적 운동은 비항산화제 섭취근의 심장근 MDA 생성을 40%정도 증가시켰으며 장기간의 복합 항산화제 섭취는 심장근의 MDA 생성을 30%정도 감소시키는 것으로 나타났다. 또한 내측 비복근의 GPx및 GR 활성도 항산화제 섭취 시 활성도가 증가되는 것으로 나타란다(P< 0.05). Cu/Zn-SOD mRNA 발현량도 항산하제를 섭취하거나 탈진적 운동을 실시하었을 경우, 일반적으로 증가되는 것으로 나타났다. Lee W.L. Effects of Exhaustive Exorcise and antioxidants suppiementation on Oxidative damage, Antioxidants Enzyme Activity and mRNA expression in Rats. Exercise Science, 12(4): 679-692, 2003. The purpose of this present study was to Investigate the effect of along-tern antioxidants supplementation on oxidative damage of heart and antioxidant enzyme activity of skeletal muscle immediately after exhaustive exorcise In rats. Forty male Sprague-Dawley rats(4 weeks old) were used as subjects. The subjects were divided into 4 groups such as, CON(control group), NAE(non antioxidants exercise group), Acantioxidants control group) of AE(antioxidants exorcise group). Antioxidants group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(200IU/kg/day), vitamin C(50mg/rat/day), vitamin B6(250ug/kg/day). β-carotene(300mg/kg/day)and selenomethionine (0.1mg/mg/day). After the diet experiment for 16 weeks, exorcise groups(NAE & AE) completed acute treadmill running(speed increased gradually to 25m/min, 15% uphill grade, ~50±10min) until exhaustion. Immediately alter exhaustive treadmill exercise, all the rats were sacrificed then, MDA of heart, SOD of soleus, GPx of gastrocnemius and GR of gastrocnemius were assayed. for the gene expression of antioxidant enzyme, Cu/Zn-SOD mRNA and Mn-SOD mRNA were assayed by RT-PCR In heart, lung, liver and soleus. As a rusult on antioxidant enzyme activity, exhaustive exercise increased MDA product by 40%, but a long-term antioxidants suppiementation decreased significantly MDA product by 30% in the heart. Further, antioxidants suppienmentation increased significantly the GPx and GR activity of gastrocnemius muscle after exhaustive exorcise(p<0.05). On the result of antioxidant enzyme gene expression, it seemed that Cu/Zn-SOD mRNA was Increased by bothe exhaustive exorcise and antioxidants supplementation

신장성과 단축성 수축 트레이닝에 따른 Muscle Peak Torque의 변화에 관한 연구

이왕록 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.1

The purpose of this study was to investigate the effect of the concenteric constraction and eccenteric constraction training on muscle peak torque. Finally, 8 subjests, who were young(mean age 21) and had not got certain disease, attended to this study. Each subject did both eccentric contraction and concentric contraction compared by left-right low leg so that we could reduce some erro which might come from the difference of muscle type composition and muscle power by an individual. Isokinetic exercise training was done 3 times per week during 10 weeks and muscle peak torque was tested at 5 weeks and 10 weeks. 30°/sec and 60°/sec were determined on angular velocity. The results were analyzed by comparating concentric constraction peak torque to eccentric constraction peak torque. Results were presented as mean±SD using a one-way repeated ANOVA. A Duncan was made for post-hoc multiple comarisons and statistical significance set at p>0.05 was treated as trends. 1. Muscle peak torque of quadriceps and hamstring were significantly increased by concentric and eccentric contraction training after 10 weeks. 2. Concentric contraction peak torque of quadriceps was significantly increased during 1st perod(5 weeks) and 2nd period (5 weeks to 10 weeks) in concentric contration training group. 3. Concentric and eccentric contraction peak torque of quadriceps were significantly increased during 1st period(5 weeks) but not 2nd period(5 weeks to 10 weeks) in eccentric constraction training group. 4. Concentric contraction peak torque of hamstring was significantly increased during 1st period(5 weeks) but not 2nd period(5 weeks to 10 weeks) in concentric contraction training group. 5. Concentric and eccentric contraction peak torque of hamstring were significantly increased during 1st period(5 weeks) but not 2nd period(5 weeks to 10 weeks) in eccentric contraction training group. 6. In the ratio of eccentric contraction peak torque devided by concentric constraction peak torque, the ratio of eccentric constraction training' was higher than that of concentric contraction training' at 5 weeks. 7. Increase of eccentric contraction peak torque was so low from 5 weeks to 10 weeks in eccentric contraction training group. In conclusion, eccentric contraction training was more effective than concentric contraction taining to improve muscle power for a short period.

스포츠 과학 : 신체활동, 운동 그리고 면역

이왕록 한국스포츠정책과학원(구 한국스포츠개발원) 2009 스포츠과학 Vol.107 No.-

운동과 라스베라트롤 투여가 고지방식이 유도 비만쥐의 지방간 섬유화, 염증발현에 미치는 영향

이왕록,박희근 한국체육과학회 2020 한국체육과학회지 Vol.29 No.3

This study was investigated to determine the effect of moderate exercise training and resveratrol supplementation on hepactic fibrosis, inflammation in high fat diet induced obese mice. Male C57BL/6 mice were randomly divided into four groups ; (NC, normal diet control; HC, high fat diet control; HRe, high fat diet with resveratrol; HME, high fat diet with moderate exercise). Exercise training was performed for 16 weeks on a treadmill running for 40-60 min/day at 10-22 m/min, 0% grade, 5 days/ week. Resveratrol supplementation group (HRe) received a daily dose of resveratrol (25mg/kg of body weight) 5 days/week for 16 weeks. As a result, body weight and α-SMA mRNA expression was significantly reduced by moderate exercise training(p<.05). TNF-αmRNA expression showed the same tendency as α-SMA(p<.05). Moreover, macrophage infiltration marker(CD11c) mRNA expression was significantly decreased by moderate exercise training(p<.05). These findings suggest that moderate exercise training has beneficial effects to hepactic fibrosis and inflammation in high fat diet-induced obese mice.

실내운동과 유산소성 복합운동에 따른 20 대 비활동자의 체력변화

이왕록,정성태 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.1

The purpose of this study was to propose a effective exercise program. Thus, we investigated the effect of Indoor and Combination Exercise Program. All subjects(mean age = 21.5), inactive people, devided into 3 groups; 1) Control Gruop. 2) Indoor Exercise Group(n=15) exercised whole body stretching, push-up, sit-up and jumping on sitting 5 times per week for 10 weeks indoor. 3) Combination Exercise Group(n=18) exercised running at 12 Km/hour(mean 70% VO_2 max) 3 times per week and whole body stretching. push-up, sit-up and jumping on sitting 5 times per week as Indoor Exercise Group for 10 weeks. All subjects executed the same diet during the experimental period. Felxible, balance, agility, power, grip strength, back strength, muscle endurance and cardiopulmonary endurance were selected as variables of physical fitness. Further, %body fat was tested by Bioelectrical Impedance Analysis. Results were presented as mean±SD using paired T-test and statistical significance set at p>0.05 was treated as trends. The result follow as: First, in Control Group(CG), there was not any significance on physical fitness variables 10 weeks later. Second, in Indoor Exercise Group(IEG), flexible, grip strength, back strength, muscle endurance and body fat were significantly changed 10 weeks later(P<0.05). Third, in Combination Exercise Group(CEG), flexible, balance, power, grip strength, back strength, muscle endurance, body fat and cardiopulmonary endurance were significantly changed 10 weeks later(P<0.05). In conclusion, only Indoor Exercise Program without exercise equipment might have positive effect on improving physical fitness. However, the combination Exercise Program or running and indoor exercise was strongly effective on improving both the health related fitness and the skill related fitness.

복합운동프로그램이 초등학생의 신체구성, 혈중지질 및 체력에 미치는 영향

이왕록,이영란,이상기,정준현,전용수,황정민 한국스포츠리서치 2006 한국 스포츠 리서치 Vol.17 No.6

탈진적 운동과 항산화제 섭취가 쥐 가자미근의 SOD 활성에 미치는 영향

이왕록 한국운동과학회 2001 운동과학 Vol.10 No.1

탈진적 운동과 항산화제 섭취가 쥐 가자미근의 SOD 활성에 미치는 영향. 운동과학, 제10권 제1호, 21-29, 2001. 본 연구는 탈진적 트레드밀 운동과 장기간의 복합 항산화제 섭취가 탈진적 트레드밀 운동 시 쥐 가자미근의 SOD 활성에 미치는 영향을 규명하고 비교·검토하는데 그 목적이 있다. 본 연구의 실험동물은 태어난 지 4주가 된 Sprague-Dawley계 횐쥐 수컷 32마리를 대상으로 하였다. 실험동물은 무작위로 각 집단에 16마리씩 2집단으로 나누어 항산화제 섭취군(Antioxidant Group)과 비섭취군(Non-antioxidant Group)으로 분류한 후 다시 각 8 마리씩 비운동군(CON, AC)과 운동군(NAE, AE)으로 나누어 실험을 수행하였다. 비 항산화 섭취군(n=16)은 16주 동안 일반 쥐 사료(Samyang, Inc., Korea)를 섭취하였으며 항산화섭취(n=20)은 비타민 E(200 IU/㎏/day), 비타민 C(5O ㎎/rat/day), 비타민 B_6(250 ㎍/㎏/day), 베타카로틴(30 ㎎/rat/day)과 셀레니움(0.1 ㎎/㎏/day)을 혼합한 사료를 같은 기간 섭취하였다. 실험 기간동안 체중을 매주 2회씩 기록하였다. 식이 처치가 끝난 16주 뒤 운동군(n=16, NAE & AE)은 쥐 트레드밀을 이용하여 탈진적 런닝(25 m/min, 경사도 15%, ∼50±10 min)을 실시하였다. 운동이 종료되면 곧 바로 쥐를 해부하여 쥐의 우측 가자미근(Soleus)을 획득하여 SOD 활성을 측정하였다. 효소 활성 분석이 끝나면 각 집단별로 평균과 표준편차를 구하고 이원변량분석을 실시하였으며 집단간 단순비교를 위하여 독립 t-test를 실시하였다. 이상의 연구 절차를 통해 얻은 연구결과는 다음과 같다. 첫째, 최대운동은 쥐 가자미근의 SOD 활성에 영향을 미치지 않았다. 둘째, 장기간의 복합 항산화제 섭취 또한 쥐 가자미 의 SOD 활성에 영향을 미치지 않는 것으로 나타났다. 이러한 결과는 가자미근이 운동으로 인한 활성 산소 및 ROS에 대처하는 항산화효소의 활성이 높음을 의미하며 나아가 항산화효소와 관련된 연구에서 논란이 되고 있는 생체조직의 종류에 따라 즉, 각 골격근의 항산화효소의 활성능력에 따라 운동으로 인한 조직의 산화적 손상이 다르게 나타난다는 것을 의미하는 것이라 판단된다.

운동과 면역

이왕록 대한운동사협회 2010 대한운동사협회 워크숍자료집 Vol.31 No.-

10주간의 지구성 운동이 자연발생고혈압쥐의 심장근 및 비복근의 IL-1ß, Mn-SOD 및 Caspase-3 유전자 발현에 미치는 영향

이왕록,전종귀,박희근,전용수,이광무,김명화,황정민 한국운동영양학회 2008 Physical Activity and Nutrition (Phys Act Nutr) Vol.12 No.1

The present research was to investigate the effect of a long-term endurance exercise training on the expression of IL- 1β, Mn-SOD and Caspase 3 in the heart and gastrocnemius of Spontaneously Hypertensive Rats (SHR). Twenty Wistar- Kyoto (WKY) rats and twenty SHRs (age, 5 weeks) were used and randomly divided into 4 groups; WKY Control (WC, n = 10), WKY exercise (WE, n = 10), SHR control (SC, n = 10) or SHR exercise (SE, n = 10). Endurance exercise was performed by a treadmill-running program (15 m/min, 0% grade, 40 mins/day, 5 days/week, 10 weeks). Total RNA was extracted from heart and gastrocnemius tissues using TRIzol method. After cDNA was synthesized, Gene expression of IL-1β, Mn-SOD and Caspase-3 were measured by Real-Time PCR, while systolic blood pressure (BP) and heart rate (HR) were monitored by tail-cuff pressure measurement for the whole experimental period. Oneway repeated ANOVA was used and p value under 0.05 was considered as statistical significance. 10 weeks running significantly inhibited the increment of systolic blood pressure in SE. IL-1β gene expression of WC (0.98 ± 0.17) and WE (0.15 ± 0.01) was significantly higher than that of SC (0.04 ± 0.008) and SE (0.04 ± 0.007) in heart. Mn-SOD gene expression of WE (26.79 ± 2.09) was significantly increased compared to other groups in heart. Caspase-3 gene expression of WE (5.90 ± 0.73) was significantly higher than that of the others. Further, Caspase-3 of WC (3.18 ± 0.51) was significantly increased compared to that of SC (0.84 ± 0.15) and SE (1.12 ± 0.16). However, IL-1β, Mn-SOD and Caspase-3 gene expression of gastrocnemius were not significant in the all groups. In conclusion. 10 weeks endurance running had positive effect on the systolic pressure, but not on the gene expression of heart in SHRs. IL-1β, Mn-SOD and Caspase-3 gene expression of heart were might impaired by chronic high blood pressure. However, IL-1β, Mn- SOD and Caspase-3 gene expression of gastrocnemius might not be affected by chronic high blood pressure.

Long-term moderate exercise training on macrophage response In mice

이왕록,박희근,정준현,전용수,권순미,이영란,황정민,윤아람,장학영 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-