소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

뇌졸중 후 중추성 통증 환자에 대한 동서협진이 진통과 재활에 미치는 영향

이현종,김수영,이상훈,서동민,이두익,김건식,이재동,이윤호,양형인,박재경,최도영 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Purpose : In order to study the effectiveness of East-West pain treatment on central poststroke pain(CPSP), we evaluated its effect on alleviation of pain and rehabilitation of CPSP Patients who were treated with electroacupuncture and west pain treatment for four weeks. Methods : Twenty four patients diagnosed by their pain characteristics of central pain from stroke were treated with sympathetic nerve block, gabapentin, amitriptyline, and electroacupuncture for four weeks. Pain intensity through the visual analogue scale(VAS), and improvements of mobility and rehabilitation through the modified Barthel index(MBI) and Rankin scale(RS), respectively, before and after pain treatment were also assessed. Results : VAS pain scores were significantly improved from 7.7±1.7 to 4.4±2.0 with pain treatment(p<0.05). In accordance with improvement of pain scores, RS and MBI scores were also improved from 2.88±0.95 to 2.13± 1.01 and from 83.0± 16.9 to 94.7±9.5(p<0.05), respectively, with pain treatment(p<0.05). Conclusions : It was suggested that the active pain treatment was contributed to the rehabilitation of CPSP patients, resulting in improvement of quality of life of CPSP patients. Futhermore, East pain treatment in combination with West pain treatment may be useful modality to alleviate CPSP.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

다중 속성의 가중치를 이용한 인터넷 채용 협상 시스템의 설계 및 구현

이근수,이근왕,이종희 안성산업대학교 2002 論文集 Vol.33 No.-

These day, Internet Recruiting needs the negotiation of recruiting items. This paper proposes multilateral negotiation that substitutes applicants and employers. Previous Negotiation Systems use the preference value of the multi-attribute and the sequential negotiation. But proposed Internet Recruiting Negotiation System(IRNS) uses the parallel negotiation of multi-attribute. The parallel negotiation supplies the multi-attribute negotiation including values of single-attribute and result of parallel negotiation. This paper shows that excellent result for effective negotiation using the weight strategy of multi-attribute for a matter of concern of users.

Southern Blot Hybridization법을 이용한 악성 림프종의 면역유전형에 관한 연구

전호종,기근홍,김윤신,이미숙,이미자,장원재,장숙진,박영진,정춘해,정종훈,양성훈,이광민 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

Immunogenotyping using gene rearrangement analysis has emerged as a precise laboratory aid in the diagnosis and classification of malignant Iymphoid neoplasms. The lineage and clonality of the malignant Iymphoid neoplasms can be identified by the demonstration of rearrangements of antigen receptor genes of the immunoglobulin and T-cell receptor genes. The analysis of the gene rearrangements on the malignant Iymphoid neoplasms are also useful as a sensitive unique clonal markers to detect early recurrence in patients with malignant Iymphoid neoplasms after treatment. To analyze the sensitivity and specificity of gene rearrangements in the diagnosis of malignant Iymphoid neoplasms. 24 cases of malignat Iymphoma were examined by Southern blot hybridization using CTβ-T cell receptor β chain gene-DNA probe and JH-immunoglobulin heavy chain gene-DNA probe. The results of the immunogenotypings using Southern blot hybridization disclosed high correlation between the immunophenotyping using immunohistochemical stain with monoclonal antibodies (B-cell Iymphoma 84.2%. T-cell Iymphoma 75% ). The analysis of the gene rearrangement of the angioimmunoblastic lymphadenopathy(AILD) and unclassifiable Iymphoma using immunohistochemical stain could resolve the monoclonality and lineage . Rearranged bands to the CTβDNA probe were observed in one case out of 2 cases of AILD. One case of unclassifiable Iymphoma showed rearranged bands to the CTβ DNA probe. There were no rearrangements in reactive follicular Iymphoid hyperplasia and paracortical Iymphoid hyperplasia. In conclusion, DNA gene rearrangement study should be applied to differentiate the clonality and cell lineage in the malignant Iymphoma with indistinctive immunophenotype.

수종의 cytokine이 배양중인 조골세포에 미치는 영향

李鐘烈,高鮮一,金正根 단국대학교 치의학연구소 1995 단국대치의학연구소논문집 Vol.7 No.1

富歸芍藥散이 白鼠의 排卵에 미치는 影響

李汀帝,金鐘桓,金哲源,柳深根 한국전통의학연구소 2000 한국전통의학지 Vol.10 No.1

Dangguijagyagsan(DGJYS) is used in infertile. An attempt was to evaluate the influences of DGJYS on the serum concentrations of FSH, LH and estrogen, the histolgical and optical changes of ovary. The results of the study were as follows : 1. Blood FSH level increased in experimental group as compared with control group on 5th day and 10th day, which showed no efficacy. 2. Blood LH level decreased in experimental group as compared with control group on 5th day and 10th day, which showed no efficacy. 3. Blood E2 level increased in experimental group as compared with control group on 5th day and 10th day, which showed no efficacy. 4. In histolgical observations of ovary, ovulation increased in experimental group as compared control group on 5th day and 10th day, which showed no efficacy. 5. In optically observations of ovary, size of ovary showed no change in exerimental group as compared with control group on 5th day and 10th day. 6. In histological observations of ovary, endometrium showed multiplication. According to these results, the effects of DGJYS are assumed to be usefully utilized for progress of ovulation on uterus and ovary.

[논문]SCP 설치지반의 압밀침하특성에 관한 실험적연구

임종절,김동호,박이근 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

바야흐로 개방화 시대를 맞이하여 세계 각국들과 교역량이 늘어나면서 여러 제반시설을 필요로 하게 되었다. 특히 항만과 같은 대규모 공사의 기초 작업으로 먼지 해야할 일은 시공 예정지역에 형성되어있는 해양의 연약점성토 지반을 개량해서 강도가 높은 안정된 기초를 가지게 하는 것이 중요하다. 토압, 지진, 파압 둥에 우수함이 이미 일본 등 여러 나라에서 검증되어 온 SCP공법은 우리의 지반조건에 맞는 설계자료가 필요하다. 이를 위해 본 연구에서는 SCP 압밀실험에서 치환율에 따라서 압축지수의 변화관계를 분석하였다.

이부성형술에서 강선 고정술과 소강판 고정술간의 안정성에 관한 임상적 연구

이은택,김수남,민승기,이동근,송종민 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

Performing genioplasty for greater stability of the changed chin position, incision, dissection, osteotomy design and fixation are important technical considerations. Basically, wire osteosynthesis method has been used in genioplasty, but plate/screw osteosynthesis has been introduced in rigid fixation. The purpose of this study is that comparison of stability between wire and plate/screw osteosynthesis in genioplasty. In this study, the genioplasty groups were divided into three groups ; advanced genioplasty group, reduction genioplasty group, advanced with reduction genioplasty group. In wire osteosynthesis groups, there were 15patients who had advanced genioplasty, 13patients who had reduction genioplasty, and 12patients who had advanced with reduction genioplasty. In plate/screw osteosynthesis groups, there were 15patients who had advanced genioplasty, 13patients who had reduction genioplasty, and 13patients who had advnaced with reduction genioplasty. Lateral cephalograms ; pre- and postoperatively, postoperatively 1months, and at the latest follow-up(>6months); were analyzed by linear measurement to evaluate changes in position(hard tissue B, Pogonion point) and compare relapse between both groups.

수종 식물추출물의 항히스타민작용

이신웅,이윤주,손종근 영남대학교 약품개발연구소 1996 영남대학교 약품개발연구소 연구업적집 Vol.6 No.-

The antihistaminic action of eighteen herbal medicines was investigated by the radioligand binding and functional assays. The hexane fractions of Trichosanthis radix, Mori cortex radicis and Evodiac fructus dose-dependently inhibited [³H]mepyramine binding to H, receptor in guinea-pig brain homogenates and histamine-induced contraction of isolated guinea-pig ileum. Antihistaminic action of the hexane and ethyl acetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus was more potent than their antimuscarinic action evaluated from the inhibition of [³H]QNB binding and carbachol response. The ethyl acetate and chloroform fractions from Scutcllariae radix also inhibited histamine-induced contraction, but antihistaminic potencies of these fractions were almost identical with their antimuscarinic potencies. The hexane fractions of Mori cortex radicis and Evodiae fructus inhibited selectively the increase of histamine-induced cutaneous vascular permeability in the rat dorsal skins. However, the ethyl acetate fraction from Scutellariae radix inhibited eqipotently the effects of histamine and serotonin on the vascular permeability. These results demonstrate that the hexane and ethyl acetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus have the selective histamine H₁ receptor blocking activity.