단클론성 감마글로부린병증에 의한 만성 탈수초성 신경병증

고진석,김승현,김주한,김명호,김덕언 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

셀룰러 망에서 이동국 속도와 방향성을 고려한 효율적인 핸드오프율

정역석,류명춘,오주환 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

In this paper we model each cell as a set of the triangle and present the handoff area using a subset of the triangle. So, handoff area can be presented depending on radius-division degree of a cell easily. we predict that a mobile station can go our or not the current cell depending on mobile station' speed, direction. We propose a calculation method of handoff rate and handoff call number using the mobile station' moving speed, direction.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

건강가정지원센터 가정생활교육 프로그램 운영 및 성과 : 용산구·숙명여자대학교 시범사업을 중심으로

김명자,계선자,박미석,장진경,김연화,한은주,류진아 한국가족관계학회 2005 한국가족관계학회지 Vol.10 No.1

The purpose of this study is to propose a model for service programs and management strategies for the division of family education in Healthy Families Center. For the purpose, the researchers find out demands for the family education programs of people in Yong-San Gu, Seoul, exam the effect of each family education program of Yong-San Gu & Sookmyung Women's University Healthy Families Center from June to December, 2004. According to the results, people in Yong-San Gu need economic education service mostly, and they want to study at Saturday afternoon. The programs show positive effect on the improvement of family health.

규칙기반 시스템과 데이터베이스 관리 시스템의 통합 기법에 관한 연구

류명춘,정영석,오주환 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

It is useful of the integration of rule systems into DBMS. Because rule-based system supports a eminent inference and analysis power and relational DBMS manages efficiently large data supporting consistency and independency. In this paper, we compares and analyzes the three approach for the integration of rule system into DBMS. Also we design inference system for rule-based database system.

바터 팽대부에 생긴 유암종 1예

강명주,지삼룡,박석주,이재익,조영완,김준영,박성재,박은택,이연재,이상혁,설상영,배상균 인제대학교 2006 仁濟醫學 Vol.27 No.-

Carcinoid tumors have been reported in a wide range of organs but most commonly involve the lungs, bronchi and gastrointestinal tract. Within the gastrointestinal tract, appendix is the most common location for carcinoid, followed by the distal small intestine, the rectum, and the stomach. Among these, primary involvement of the ampulla of Vater is extremely rare. We report a case of carcinoid tumor of ampulla of Vater. A 62-year-old man presented with epigastric soreness. Gastroduodenal endoscopy showed hyperemic bulging mass on ampulla of Vater and diagnosed as carcinoid tumor by histologic finding, with immunohistochemical study. In ^(111)In-octreoscan, tumor had regional lymph node metastasis, but no evidence of distant metastasis. Pancreatoduodenectomy with lymph node dissection was performed.

간세포암의 고주파 열치료 후 천자침 경로를 통한 전이 1례

조영완,박석주,진한영,김준영,이재익,강명주,박정하,윤정희,박성재,지삼룡,이연재,이상혁,설상영 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiofrequency ablation(RFA), as a form of minimally invasive therapy for hepatocellular carcinoma, has become an important treatment modality. Because of limitation of surgery, RFA has become standard therapy for hepatocellular carcinoma in some situations. But there are some complications of RFA such as bleeding, infection, hematoma, adjacent organ thermal damage including intestinal perforation, needle track seeding, and so on. There are few reports in the literature that systematically evaluate the incidence of needle tract seeding and its associated risk factors. And only 2 cases of needle tract seeding of hepatocellular carcinoma after RFA were reported in Korea. We report a case of needle tract seeding of hepatocellular carcinoma after RFA.

내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가

이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.

안구적출에 따른 위둔덕의 칼슘결합단백질의 재구축 및 상호 연관성

안병수,고길석,안명수,김경주,권안성,정명섭,박춘매,조병옥,김진우,Samudra Acharya,Parmeshwar Narayan Amatya,장인엽 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background: Superior colliculus is a part of midbrain, and participates in the visual reflexes, It receives afferent fibers from optic nerve, visual cortex, and spinotectal tract. After optic deprivation, the microscopic structure of the superior colliculus changed. Calcium-binding proteins (CBPs) Play an important role in the neuronal protection, differentiation and reorganization of the central nervous system, Objectives and Methods: The effects of neonatal retinal deafferentation on a CBPs, calbindm D-28k (CB), Parvalbumin (PB) and calretimn (CR), and the existence of colocalization between the CBPs were examined immunohistochemically in the rat superior colliculus. Results: On the experimental (contralateral to enucleation) side of superior colliculus, the number of CB-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. The number of PB-IR neurons and fibers was also reduced on the experimental side (88.5% compared to control), In the other hand, the CR-IR cells were dramatically increased (642% compared to control), but CR-IR fibers were markedly decreased on the experimental side. The colocalization between CB-CR and PV-CR was rarely observed in the superior colliculus Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively stable in CB- and PV-IR neurons and plastic in CR-IR neurons.

Competitive PCR법을 이용한 Marine birnavirus(MABV)의 정량적 검출

김영진,김석렬,정성주,오명주 한국어병학회 2001 한국어병학회지 Vol.14 No.3

기존의 어류 병원성 바이러스 연구에 있어 주화세포를 이용한 TCID_50과 plaque assay는 미량의 바이러스에 대해, 그리고 현재 많이 사용되고 있는 polymerase chain reaction(PCR)은 정확한 정량이 힘들다는 것이 각각 단점으로 지적된 바 있다. 이에 어류 병원 바이러스를 효율적으로 조사하기 위한 방법을 개발하고자 연안 양식장에 만연해 있는 marine birnavirus(MABV)를 대상으로 competitive PCR을 이용하여 그 감도 및 정량성 여부를 시험하였다. 본 연구에서 1.44×10^7 PFU/㎖의 바이러스 액에 대해 10^4배 희석한 바이러스 시료까지 검출이 가능하였고, 이 바이러스 액 200㎕에는 약 100 copy의 MABV cDNA가 존재함을 확인할 수 있어 이와 같은 바이러스 유전자의 양을 토대로 간접적인 추정이 가능할 것으로 판단되었다. 감염된 개체에 있는 viral genome의 수는 질병의 진행을 monitor하는 데 중요한 parameter의 하나로서 이 방법을 이용하여 얻어진 정보는 infection stage의 구분에 대한 기초자료로 이용할 수 있을 것으로 판단된다. It has been shown that TCID_50 and plaque assay might be hard to apply for the analysis of small amount of virus. Additionally polymerase chain reaction(PCR) does reply the quantity of virus correctly. Thus we developed the competitve PCR, and effective mean to investigate fish pathogenic virus, and analyzed the sensitivity against marine birnavirus (MABV). In this study, the virus sample of 1.44×10^7PFU/㎖ was detected after up to 10^4 dilution. And the existence of 100 copies MABV cDNA was identified with the competitive PCR in this virus sample. These results indicated that the indirect presumption was available on the basis of the amounts the viral genome. Viral genome's number in the infected individual can be used as the fundamental data to divide the infection stage and to monitor the progress of disease.