3,4‑Dichloroaniline promotes fatty liver in zebrafish larvae

Ji‑Seon Park,송정아,Jong‑Su Park,이상우,Jieon Lee,Han‑Jin Park,Woo‑Keun Kim,Seokjoo Yoon,Hang‑Suk Chun 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Backgrounds 3,4-Dichloroaniline (3,4-DCA) is a transformation product of herbicides that is commonly used as a reference in developmental toxicity studies (OECD TG 236) (Bonnet et al. in Environ Toxicol 22:78–91, 2007). However, the mechanisms underlying 3,4-DCA-induced hepatotoxicity are not well known. Methods We exposed zebrafish larvae at 72 hpf to 3,4-DCA for 3 days and observed lipid accumulation in liver treated with 10-μM 3,4-DCA using oil red O staining. Subsequently, we performed qRT-PCR analysis to determine the genes involved in the observed lipid accumulation. Results We found that genes related to lipogenesis (srebp1, pparγ, lipin1, and scd1) and ER stress (bip, atf4, ddit3, dnajc3, and edem1) were significantly upregulated. In addition, we found that ROS generation increased in the larvae treated with 10-μM 3,4-DCA. Moreover, glutathione-S-transferase activity in these larvae was increased by 3,4-DCA in a dose-dependent manner, and the expression of the inflammation marker il-1β increased. Conclusion Our results indicated that exposure to 3,4-DCA induced fatty liver in zebrafish larvae and that this, in association with additional factors such as ER stress response, can promote liver damage. We accordingly suggest that 3,4-DCA could be used to induce fatty liver in zebrafish larvae.

Encephalomyocarditis Virus 표면항원의 단일항체생산 세포주의 크론과 이의 면역학 및 생화학적 연구(I)

윤장현,이숙영,김우제,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

To determine the immunological and biochemical properties of monoclonal antibody against encephalomyocardins viral surface antigen, Balb/c female mice were immuunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with niyeloma cells(NS-l). Sixteen monocloiial antibody-producing hybridoma cell lines were selected from non-producing hybridoma cell lines. Among them, three hybridoma cell lines produced IgG2b and other thirteen hybridoma cell lines produced IgG1. Two hybridoma cell lines, EDH-S-23 and EDH-S-24, Produced IgG2b and showed highest neutralization titer, 236. The antigenic specificity of these monoclonal antibodies was specific to D-variant of encephalomyocarditis virus. VIoreover, the monoclonal antibody showed specifically the curring effect on diabetes mellitus of mice, which was induced by the D-variant of encephalomyocarditis virus.

항정신병 약물이 Cyclooxygenase-2 유전자 발현과 Prostaglandin E_2 농도에 미치는 영향

김종우,조성욱,범재명,윤도준,장환일,송지영 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.2

Objectives : Cyclooxygenase(COX) is the central enzyme in the biosynthetic pathway to prostaglandins from arachidonic acids. The action mechanism of nonsteroidal anti-inflammatory drugs is through inhibition of COX activity and prostanoid biosynthesis including prostaglandin E_2(PGE_2). The antipsychotic drugs are known having an analgesic effects, however its mechanisms of actions of action to pain control was not still clearly proved. In the present study, the effects of antipsychotic drugs(chlorpromazie and clozapine) on the production of PGE_2 and the expression of COX-2mRNA and protein in P815 cell line were studied. Methods : For that purpose, the methods of RT-PCR, western blot and prostagalndin E_2 enzymeimmunoassay were applied. Results : The results showed tha tin P815 cells treated with chlorpromazine, the level of COX2 mRNA was significantly decreased compared to the control cells. And total cellular PGE_2 levels were significantly decreased after treatment with chlorpromazine and clozapine. Conclusion : These results suggest that antipsychotic drugs have revealed their analgesic effect through the inhibition of COX-2 activity and PGE_2 production. And also, these results partly supports the excess theory of prostaglangdins in the pathogenesis of schizophrenia.

최적화 설계문제를 위한 유전알고리즘에 관한 연구

이성우 ; 박지원 ; 윤영수 김천대학교 1996 김천대학교 논문집 Vol.17 No.-

Methods to find an optimal solution that is the function of the design variables satisfying all constraints have been studied, there are still many difficulties to apply them to optimal design problems. A method to solve the above difficulties is developed by using Genetic Algorithm, it has been successfully applied to various prob-lems with a mix of continuous, integer and discrete design variables that could not have been readily solved with traditional computa-tional techniques, but several problems for which conventional Genetic Algorithm is ill defined are premature convergence of solu-tion and application of exterior penalty function. Thus, we devel-oped an Improved Genetic Algorithm (IGA) to solve above two prob-lems.

호르몬 불응성 전립선암 환자에서 mitoxantrone과 prednisone의 병합화학요법 후 통증완화 및 삶의 질 측정

이홍우,이지열,김세웅,이충범,강성학,조용현,황태곤,박용현,윤문수 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 호르몬 불응성 전립선암 환자의 생명을 연장시키는 치료법은 현재로는 없으며, 현 시점에서의 치료목표는 증상의 경감 및 소실에 따른 삶의 질 향상에 두고 있다. 호르몬 불응성 전립선암의 고식적인 치료에 일차적인 치료법으로 간주되는 mitoxantrone과 prednisone의 화학요법이 호르몬 불응성 전립선암 환자의 통증완화 및 삶의 질에 어떠한 영향을 미치는지 알아보았다. 방법 : 1998년 7월부터 1999년 12월까지 증상이 있는 18명의 호르몬 불응성 전립선암 환자를 대상으로 하였으며, 일차적인 치료의 목표는 환자 본인이 기록하는 McGill-Melzack 6단계 통증척도에서 진통제의 증량 없이 2점이 감소(또는 첫 점수가 1점일 경우 통증의 완전소실)하는 것으로 하였다. 이러한 기준은 최소 3주 간격으로 시행한 자가 기록조사에서 연속적으로 2번 이상 유지될 때 유효한 것으로 간주하였다. 건강과 관련된 삶의 질 측정은 전신적 치료의 임상적 응용을 위한 실제적인 측정방법으로 개발되어 환자가 직접 작성하는 전립선암 특이성 삶의 질 측정기구(Prostate Cancer-Specific Quality-of-Life Instrument : PROSQOLI)의 비례연속형의 자가측정 척도(linear analogue self-assessment scale : LASA)를 이용하였으며, ECOG performance status를 사용하여 전신상태의 변화도 측정하였다. 결과 : 통증의 경감으로 나타나는 고식적인 치료의 반응은 66.7%(12/18)에서 나타났으며, 반응의 지속기간은 2개월에서 10개월까지로 평균 4.2±3.6개월이었다. PROSQOLI로 측정한 삶의 질은 통증에 대한 고식적인 치료반응을 보인 12명(66.7%) 모두가 삶의 질이 개선되었으며 특히 통증, 변비, 소변보기, 전반적인 복지상태에서의 유의한(p<0.05) 증가를 보였다. 치료 후 혈장 PSA 수치는 고식적인 반응의 유무에 관계없이 별 다른 변화를 보이지 않았으며, 골주사 또는 전산화 단층촬영으로 측정한 방사선학적 병변은 고식적인 반응을 보인 2명을 제외하고 치료 전과 비교하여 차이를 보이지 않았다. 병합 화학요법과 관련된 특별한 부작용은 관찰되지 않았다. 결론 : 통증을 동반한 호르몬 불응성 전립선암에서 mitoxantrone과 prednisone의 병합화학요법은 66.7%의 환자에서 통증의 감소 및 삶의 질이 향상되었다. 현재는 혈장 PSA 수치, 병소의 크기나 개수등이 치료의 지침이나 치료결과의 판정을 위한 객관적인 기준으로 사용되는 경향이 있지만, 앞으로는 환자를 중심으로 통증을 포함한 삶의 질 측정이 고식적 치료 후 치료결과 판정에 있어 객관적이고 적절한 방법이 될 것이다. Background : There is no evidence that therapeutic modalities prolong the survival of patients with hormone-refractory prostate cancer nowadays. The main goal of treatment is therefore improvement in quality-of-life including palliation of symptoms. We performed this study to investigate the benefit of mitoxantrone and prednisone chemotherapy in patients with symptomatic hormone-refractory prostate cancer using relevant end points of palliation regarding to pain and quality-of-life. Methods : From July 1998 to December 1999, we assessed the 18 patients with symptomatic hormone-refractory prostate cancer receiving mitoxantrone and low dose prednisone. The treatment end point was a palliative response defined as a 2-point decrease in pain assessed by a 6-point pain scale complemented by patients (or complete loss of pain if initially 1+) without an increase in analgesic medication and maintained for two consecutive evaluations at least 3 weeks apart. Health-related quality-of-life was evaluated with a series of linear analogue self-assessment(LASA) scale of the Prostate Cancer-Specific Quality-of-Life Instrument (PROSQOLI) and performance status was also measured. Results : Palliative responses were observed in 12(66.7%) of 18 patients. The duration of palliative responses were 4.2±3.6 months(range: 2 to 10 months). Treatment was well tolerated without specific side effects. There were no differences in prostate-specific antigen (PSA) level following treatment and no significant changes of radiologic findings evaluated by bone scan and/or CT were noted except 2 cases after chemotherapy. All the responding patients had an improvement in quality-of-life scales and performance status, however serum PSA levels were not changed. Conclusions : Chemotherapy with mitoxantrone and prednisone provides palliation and an improvement in quality-of-life for more than half (66.7%) of the patients with symptomatic hormone-refractory prostate cancer.

하악 매복 지치의 외과적 발거시 Methylprednisolone의 안면 부종 감소 효과에 대한 임상적 연구

신지훈,박진배,한지용,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : The third molars, expecially mandibular wisdom teeth, are commonly obliquely impacted, and surgical procedure with odontomy and osteotomy is necessary for their extraction. The surgical extracion of impacted wisdom teeth occasionally in duces variable discomfort. Especially, postoperative swelling, pain and limitation of mouth opening determine the degree of discomfort. For the reduction of these complicaions, drug therapy(antihistamine, enzyme, steroid), cole pack and pressure technique have been studied. It is known that the steroids are the most effective. Methods and Meterial : We selected fifty patients in their twenties, who visited Department of Dentistry, Pusan Paik Hospital from March to August in 1998. All of 50 patients has no other medical problems. Total 50 patients were randomly divided to 30 of experimental group and 20 of control group. The experimental group had orally administration of methyprednisolone 5㎎ every 8 hours 2 times preoperatively and 7 times postoperatively. Five points were marked on the patients' face, and the distances between the points were measured just before operation and 48 hours after operation. Results : Methyprednisolone reduces swelling about 50% after surgical extraction of obliquely impacted mandibular third molars with osteotomy and odontomy. The difference in the amount of tissue edema between experimental and control group was significant(p<0.01). However, there was no significant difference according to the operation time. Conclusion : It seems that the use of methylprednisolone is effective to reduce postoperative swelling following surgical extraction of third molars.

900 MHz 대역 CMOS 가변이득 증폭기 설계

이지호,채규성,윤상웅,김창우 慶熙大學校 레이저 工學硏究所 2007 레이저공학 Vol.18 No.-

A vairable gain amplifier(VGA) has been designed for 900 MHz-band applications. The VGA consist of three blocks; a control voltage feeding block, variable gain block, and constant gain block. The VGA has been simulated using the TSMC CMOS libraries. At 900 MHz, the maximum gain is 34.6 dB with an 1 - dB gain compression output power of 3 dBm. The gain control range is 30 dB from 3.8 dB to 34.6 dB by the control voltage from 0.9 to 3.0 V. The gain control sensitivity of the VGA is 70 mV/dB, which is larger than a thermal voltage (26 mV/dB at the room temperature) in order to be insensitive to temperature variation.

Hollow-Fiber LPME를 이용한 잔류농약 검출

조현우,이상섭,신윤지,장미애 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-

A method for the determination of trace amount of pesticides in aqueous samples using hollow fiber-liquid phase microextraction(HF-LPME) and capillary gas chromatograph/micro electron capture detector(GC/μ ECD) has been established. HF-LPME method has been optimized with respect to several experimental parameters including the effects of extraction solvent, extraction time and extraction temperature for the determination of pesticides from aqueous samples. The calibration curves for pesticides showed good linearity (above r²=0.98). The established method is feasible for the determination of trace amounts of pesticides in several aqueous sample. The limit of detection(LOD) and the limit of quantitation(LOQ) have been found to be 0.05~1.0 and 0.5~2.5 ng/mL, respectively. The established HF-LPME method for the analysis of pesticides from aqueous sample can be used for the determination of biological, food and environmental samples.

백혈병환자에서 Liposomal Amphotericin B로 치료된 파종성 Fusarium 감염 1예

이지연,김성범,박대원,신성희,김용현,윤소영,엄중식,최철원,정희진,김우주,김준석,김장수,이갑노 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

Fusarium spp.는 사람에서 드물게 각막염, 조갑진균증, 상처나 화상부위감염 등을 유발하는 것으로 알려져 있으나 최근에는 면역저하자에서 기회감염의 원인균으로 증가하고 있는 추세이다. 면역저하자에서 파종성 감염이 있는 경우에 예후는 치명적이나, 증례가 많지 않고 진단방법이 제한되어 있어 조기 진단과 신속한 치료가 쉽지 않다. 그러나 특징적으로 피부병변의 동반이 흔하여 면역저하자에서는 피부 병변의 면밀한 관찰과 함께 Fusarium 감염을 조기에 의심하여 피부생검이나 배양을 하는 것이 진단에 도움이 되리라 생각된다. 파종성 Fusarium 감염의 치료로는 감염 부위의 국소적 외과적 절제와 전신적 항진균제의 사용이 필요하고 현재까지는 amphotericin B가 비교적 효율적인 항진균제로 알려져 있으나 면역저하자에서 이에 반응을 하지 않는 경우가 많다. 저자들은 급성골수성백혈병 환자에서 관해유도화학요법 시행 후 호중구감소상태에서 피부병변과 폐침윤이 발견되었고 피부의 중심부 궤양을 동반한 구진성 병변에서 배양검사를 통해 파종성 Fusarium 감염을 진단하였으며 liposomal amphotericin B (AmBisome )를 사용하여 성공적으로 치료한 예를 경험하여 보고하는 바이다. Fusarium spp., basically a superficial pathogen, is a newly emerging fungal pathogen of opportunistic infections in immunocompromised patients. At present, although Fusarium spp. are relatively resistant to amphotericin B, the combination of amphotericin B and surgical debridement appear to be optimal treatment for disseminated infection. Recently we experienced a 32-year-old neutropenic patient after induction chemotherapy for acute myelocytic leukemia presented with skin lesions and infiltrations in both lungs. We diagnosed with disseminated fusariosis by skin culture and successfully treated the patient with liposomal amphotericin B. We emphasize a high index of suspicion for skin lesions especially in immunocopromised patients.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.