公企業會計制度의 事例硏究 : 水道事業의 實證的 分析을 中心으로 An Analysis of Piped Water Works in a Local dcity

尹永彩,車在德 全北大學校 1967 論文集 Vol.9 No.-

Prednisolone과 Prednisone의 임상약동학적 평가

신재국,윤영란,차인준,이경훈,장인진,신상구,박찬웅 大韓臨床藥理學會 1994 臨床藥理學會誌 Vol.2 No.1

일시적인 피질맹과 인지기능장애로 발현한 간성 뇌병증 1예

윤고운,박민정,박경원,차재관,김재우 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

A 55-year-old woman presented with transient cortical blindness and multiple cognitive impairments as initial symptoms of hepatic encephalopathy. Her past history was negative for chronic liver disease or gastrointestinal bleeding. Neurologic examinations showed bilateral visual loss with a normal pupillary reflex to light and neuropsychological test showed significant impairments in all cognitive domains. Laboratory tests revealed increased liver enzymes, ammonia level, and positive IgG HbcAb. Ultrsonography of the liver showed findings consistent with liver cirrhosis with splenomegaly. The visual evoked potential study resulted in no wave formation on both sides. T1-weighted brain MRI showed high signal intensity lesions in both globus pallidus and cerebral peduncles of the midbrain. After treatment with lactulose, cortical blindness and cognitive dysfunctions disappeared within several days. In rare cases of hepatic encephalopathy, cortical blindness with cognitive impairments may occur before alteration of consciousness and usually recovers following treatment of the hepatic encephalopathy.

흰쥐에서 paraquat독성에 미치는 tumor necrosis factor-α의 영향

차종희,박정수,양성훈,박재윤,이병래 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background : The toxicity of paraquat is due to the oxygen-derived radicals formed by the reaction of oxygen with bipyridylium radical cations. Tumor necrosis factor (TNF)-α induces the synthesis of mitochondrial manganese superoxide dismutase (Mn-SOD) and other protective proteins in a variety of tumor cells and these protein contribute to a protect tumor cells from free radical injury. In this study, the effects of TNF-α on paraquat-induced toxicity were investigated in rats. Methods : Experimental animal used was Sprague-Dawley male rat, body weight 150-200g. The paraquat toxicity was determined by survival rate. The SOD, catalse, and glutathione peroxidase(GSHPx) activity were spectrophtomerically determined by Crapo method, Aebi method and Flohe method, respectively. Results : The survival rate of paraquat-intoxicated rats was decreased by subctaneous injection of TNF-α. However, the survival rate of paraquat injected rats were increased by pretreatment of TNF-α, comparing to saline pretreatment. The liver SOD activity was increased significantly by TNF-α treatment, but kidney and lung enzyme was unaltered in TNF-α treated-rats, comparing to normal control rats. The increment of liver SOD activity by TNF-α treatment was dose- and time-dependant SOD activity of kidney and lung of rats were not changed by TNF-α injection. The activities of catalase and glutathione peroxidase and contents of thiobarbituric acid reactive substance(TBARS) in liver were not changed by TNF-α treatment. Conclusion : From these result, TNF-α potentiate paraquat toxicity in rats but TNF-α pretreatment reduce paraquat induced toxicity. Protective effect may be resulted from the increased liver SOD activities by TNF-α.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

척추손상 쥐의 지방유래 줄기세포와 미노사이클린의 운동기능 회복효과

장재혁,고현윤,차영선,박형욱,신용범,손현주 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

칼로리량 급여 제한이 OLETF 흰쥐의 콜레스테롤량에 미치는 영향

박재윤,김태형,이병래,차종희 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1

본 실험은 식이 제한 급여에 따른 비만형 당뇨병 환자의 체중 및 혈중 지질량의 변화에 대한 연구의 일환으로 사람의 비만형 당뇨병과 유사한 병리소견을 나타내는 OLETF 흰쥐를 이용하여 칼로리 급여량 비 제한군, 칼로리 급여량 20% 제한군과 40% 제한군으로 나누어 12주간 사육한 후 체중과 혈당, 혈액 cholesterol 및 중성지질량을 측정하여 칼로리 급여량제한에 따른 혈액 지질량의 변화를 관찰하였다. 체중은 칼로리 급여량 20% 제한군에서는 체중증가 억제 비율이 LETO와 OLETF흰쥐가 비슷하였고, 칼로리 급여량 40% 제한군에서는 OLETF 흰쥐에서 체중 증가 억제가 더 크게 나타났다. 칼로리 급여량 제한에 의한 혈당량의 저하는 비만형 당뇨쥐인 OLETF 증 흰쥐가 LETO 흰쥐에 비하여 더 크게 나타났다. 칼로리 급여량 제한으로 OLETF 종 흰쥐에서 혈액 중성지질량, 총 콜레스테롤량 및 LDL- 콜레스테롤량이 감소되었고, HDL-콜레스테롤량은 증가되므로, 비만형 당뇨병환자에서 칼로리 제한 식사는 OLETF 종 흰쥐에서와 유사한 혈액 콜레스테롤량의 변화를 유발시켜서 혈관 합병증을 억제할 수 있을 것으로 예상된다. 이상의 실험결과로서 칼로리 급여량 제한에 의한 체중, 혈당, 혈액 총 콜레스테롤량, LDL-콜레스테롤 및 HDL-콜레스테롤량의 변화가 비당뇨 흰쥐보다 당뇨흰쥐에서 더 큰 변화가 있음이 확인되어 칼로리 제한 급여는 당뇨병에서 체중, 혈당, 콜레스테롤량의 조절에 유의한 것으로 생각되고, 칼로리 급여 20% 제한군과 40% 제한군 사이에서 체중, 혈당, 콜레스테롤량의 변화는 칼로리 제한량의 변화에 비하여 적기 때문에 적절한 체중, 혈당, 혈중 지질량의 유지를 위해서는 칼로리 급여량을 적절히 유지하는 것이 중요할 것으로 생각된다. 또한 칼로리 급여 제한에 의한 체중, 혈당, 혈중 중성지질과 콜레스테롤량의 감소는 혈관 합병증 발생이 예방 할 수 있을 것으로 추측되는데, 이에 대해서는 계속적인 연구가 있어야 할 것으로 생각된다. Objective: To determine the effects of caloric restriction on blood cholesterol levels of obese type 2 diabetes, we measured blood glucose and cholesterol level in Otsuka Long Evans Tokushima Fatty(OLETF) rats after caloric restriction. Methods: OLETF rats(obese diabetic rats) and Long Evans Tokushima Otsuka(LETO) rats(nondiabetic control rats) were grouped into 3 groups, control group, 20% caloric restriction(20% CR) group and 40% caloric restriction(40% CR) group, respectively. The blood glucose and cholesterol level in OLETF and LETO rats were determined after 12 weeks caloric restriction. The levels of triglyceride(TG) and cholesterol(total cholesterol, LDL-cholesterol, HDL-cholesterol) in blood of rats were determined by automatic biochemical analyser(Hitachi 747 Japan). Results: Blood glucose level of LETO rats were decreased by 12% on 40% CR compared to control group but the differences of blood glucose levels between control group and 20% CR group was not observed. OLETF rats blood glucose levels were decreased by 20% in 40% CR group and by 15% in 20% CR group. The levels of blood triglyceride in 20% CR and 40% CR groups were decreased by 20%, 15% in LETO rats and by 37%, 32% in OLETF rats respectively. Total cholesterol level was not changed by dietary restriction in LETO rats, but in OLETF rats, total cholesterol levels was decreased significantly by caloric restriction. The HDL-cholesterol levels were increased while LDL-cholesterol levels were decreased by dietary restriction in both LETO and OLETF rats. Conclusion: These results suggested that OLETF rats may be more susceptive to changes of body weight and blood glucose, triglyceride and cholesterol levels than LETO rats by caloric restriction. Reduced LDL-cholesterol and triglyceride levels in diabetes by caloric restriction may have some roles on prevention of diabetic vascular complication.

오렌지 껍질로부터 분리된 프라보노이드의 화학요법 감작효과

최철익,변익건,차윤정,김선필,민영돈,유진철,송재경 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : The overexpression of P-glycoprotein (Pgp) or multidrug resistanceassociated protein (MRP) confers multidrug resistance (MDR) to cancer cells. MDR cells could be sensitized to anticancer drugs when treated concomitantly with a chemosensitizer. In this study, orange peel have been screened for the development of chemosensitizers reversing MDR. Materials and methods : Expression of Pgp in AML-2/WT and AML-2/D100 were determined using western blot analysis and RT-PCR. Candidates for chemosensitizers were isolated, purified from orange peel using sephadex LH-20 column chromatography and silica-gel column chromatography, whose structure was determined using ^1H-NMR, ^13C-NMR, ^1H-^1H-COSY, 13C-NMR, EI-Mass, IR. Chemosensitizing effect of NP-28 on AML-2/DX100 over expressing Pgp and MRP was determined by the MTT assay. Membrane toxicity was evaluated by the RBC hemolysis test. Antioxidant effects were examined by using a fluorescence probe (2’-7’-dichlorofluorescein diacetate). Results : A potent candidate, NP-28, has a flavonoid nucleus with some methoxy moiety. NP-28 is believed to have high therapeutic index, being non-transportable inhibitor and possibly, of inhibiting other efflux pumps such as MRP and antioxidant effects. NP-28 is also unlikely to be a substrate of Pgp. NP-28 did not increase Pgp levels. Conclusion : NP-28 has a Chemosensitizing effect to reverse Pgp-mediated MDR by increasing the intracellular accumulation of drugs. Furthermore, they do not increase expression of Pgp, which can allow a long term use without the concern about Pgp activation. NP-28 is anticipated as an ideal second-generation flavonoid chemosensitizer and/or possibly other therapeutic agents by further investigation.

STD 61종 금형강의 이온질화처리에 관한 연구(Ⅰ)

안병만,김용길,차병묵,피윤섭,윤재홍,김진관 國立 昌原大學校 産業技術硏究所 1994 産技硏論文集 Vol.8 No.-

STD61종 열간금형강의 수명향상을 위하여 ion nitriding처리를 실시하였다. 이온질화전의 모재의 열처리시에는 1030˚C에서 quenching한 후 530˚C에서 2시간 tempering했을 때 Hv=600의 높은 경도값을 나타내었다. 580˚C, 600˚C에서 2시간 tempering했을 때는 각각 Hv=500, Hv=400으로 경도값이 오히려 감소했다. 이온질화 처리시에는 처리시간이 길고 N₂와 Ar첨가유량이 많을 수록 깊은 화합물층과 확산층을 얻었다. 그리고 각 처리조건에서 안정된 Hv=1150의 질화물층을 얻을 수 있다. Ion nitriding treatment has been practiced to improve the life of hot die steel of STD61. Before nitriding the parent STD61 tempered at 530˚C, 2hrs after quenching at 1030˚C, It showed the high hardness distribution of Hv=600. The hardness distribution has decreased by Hv=400, Hv=500 when tempered for 2hrs at 580˚C, 600˚C. In the ion nitriding, the more treat-time and the more N₂and Ar flux, the deeper the compounded layer and the diffused layer. The stable hardness, Hv=1150, of nitride layer was obtained at various treatment condition.

녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

임성훈,서정순,윤영주,김광원,유소영,김화숙,국중기,이병래,차종희,박재윤 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.5

본 연구는 치아우식증에 관련된 Mutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연 생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 50% 에탄올을 이용하여 얻었다. 이들 추출물의 Mutans streptococci 표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장억제농도는 5 mg/ml이었다. 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다. Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatment. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casio tora were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5 mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea may be more effective than the tea prepared from C. tora in the prevention of enamel decalcification or dental caries around brackets.