원발성 난관융모상피암 1예

유진철,유성호,한영,백상붕,배국환 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.1

국방정보시스템에서의 랜섬웨어 위협 대응방안 : 정보보안 위험관리 관점에서

유진철,문상우,김종화 한국융합보안학회 2020 융합보안 논문지 Vol.20 No.5

지난해에 이어 랜섬웨어로 인한 피해가 계속 증가되고 있으나 군내 사이버작전 수행지침에 별도의 랜섬웨어 유형 분류없이 사이버 작전 상황을 관리하고 있는 실정이다. 그러나 랜섬웨어는 다른 악성코드와 달리 조치내용과 파급력을 고려할 때, 한 순간 모든 국방업무를 마비시킬 수 있는 위협요소로 군은 랜섬웨어를 재평가하고 이에 따른 대비책을 강구해야 한다. 이에 따라 본 논문에서는 정보보안 위험관리 기반의 국방정보화 관련 자산, 취약점, 위협 등을 분석하고, 랜섬웨어 위협으로부터 국 방업무의 연속성을 확보하기 위한 대안을 제시하고자 한다. Damage caused by ransomware has continued to increase since last year, but cyber operations are managed without any separate classification of ransomware types in the military's guidelines for carrying out cyber operations. However, unlike other malware, ransomware is a threat that could paralyze all defense operations in one moment, and the military should reevaluate ransomware and take countermeasures. Accordingly, this paper aims to analyze the assets, vulnerabilities, and threats related to defense information service based on information security risk management, and propose alternatives to ensure continuity of defense work from ransomware threats.

Streptomyces tubercidicus에서 Adenine과 Histidine에 의한 Tubercidin 생합성 조절

유진철,하영칠 한국미생물학회 1991 미생물학회지 Vol.29 No.3

The regulatory mechanism of tubercidin biosynthesis in Streptomyces tubercidicus was studied. In a wild type strain, addition of adenine and histidine into the medium decreased the tubercidin production by 60-65% and 40%, respectively. The effects of adenine and histidine were alleviated by the addition of inosine monophosphate and 5-aminoimidazole-4-carboxamide ribotide. The production of tubercidin in S. tubercidicus K115 strain ($ade^{-}$ ) was nearly shut off by histidine. In contrast with K115 strain, adenine inhibited the tubercidin biosynthesis in S. tubercidicus K412 strain ($his^{-}$. In S. tubercidicus F667 strain ($ade^{-}$ , $his^{-}$ ), tubercidin production was increased by adenine and histidine. From the effects of adenine and histidine on tubercidin biosynthesis in S. tubercidicus wild type and mutant strains, it became known that feedback control by adenine and histidine of biosynthetic pathwat for purine ribonucleotide and histidine are involved in the regulation of tubercidin biosynthesis.

Synthesis and Anti-HIV Activity of Novel 4'-Ethyl-5'-norcarbocyclic Adenosine Phosphonic Acid Analogues

유진철,Hua Li,이원재,홍준희 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

Novel 4'-ethyl-5'-norcarbocyclic adenosine phosphonic acid analogues were synthesized from propionaldehyde 5through a de novo acyclic synthetic route using reiterative Grignard additions and ring-closing metathesis (RCM) as key reactions. The synthesized nucleoside phosphonic acids analogues 17, 18, 19, and 21 were subjected to antiviral screening against human immunodeficiency virus.

Production and Biological Activity of Laidlomycin, Anti-MRSA/VRE Antibiotic from Streptomyces sp. CS684

유진철,Jun Ho Kim,하정완,Nae Soo Park,송재경,June Woo Lee,박성찬,Mi Sun Kim,성치남 한국미생물학회 2007 The journal of microbiology Vol.45 No.1

Culture broth of a streptomycete isolate, Streptomyces sp. CS684 showed antibacterial activity on methicilin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE). Among purified substances from the organism, CSU-1, which is active against MRSA and VRE, is a C37H62O12Na (M+,721.3875), and identified as laidlomycin. The anti-MRSA and anti-VRE activity of CSU-1 was stronger than oxacillin and vancomycin. Phylogenetic analysis showed that strain CS684 is very similar to Streptomyces ardus NRRL 2817T, whereas the ability of Streptomyces sp. CS684 to produce laidlomycin was shown to be unique.

군용 스마트폰 어플리케이션의 활용 분야와 보안 제한 사항

유진철,한창희,신규용,박병철,신진희,이종덕,유동희 육군사관학교 화랑대연구소 2012 한국군사학논집 Vol.68 No.2

The widespread diffusion of smartphones has turned out to be the main source of growth in various private sectors, and the smartphone has become an indispensable equipment in our daily life. In case of South Korea, the majority of smartphones have been used in the field of non-military, and its military applications have not been actively studied thus far. This shows a clear contrast with the fact that active researches on the adaptation of smartphones into military domain are being conducted in many advanced countries. To this end, in this paper, we first investigated the basic concepts of the smartphone, its domestic dissemination rate, related technologies, and the status of the military smartphone applications in other countries. Then, we suggested a framework for the domestic military smartphone applications in order to describe how the smartphone applications can be used in the domestic military domain. We also developed a mobile learning application prototype for preliminary rifle instruction (PRI) to demonstrate the possible use of the smartphone applications in military domain. In addition, we discussed various security issues related to smartphone application. The results of this paper will provide helpful guidelines to future researchers when they develop a variety of smartphone applications in military domain.

Microbial Phospholipase D

유진철 한국공업화학회 2007 한국공업화학회 연구논문 초록집 Vol.2007 No.-

Streptomyces tubercidicus ATCC 25502의 원형질체 생성 및 환원

유진철 조선대학교 약학연구소 1990 藥學硏究誌 Vol.12 No.1

Protoplasts isolation and their reversion to a filamentous state were studied in Streptomyces tubercidicus ATCC 25502. High yield of protoplasts were obtained from mycelia grown in S medium containing 2% glycine. Protoplast isolation from mycelia was most efficient when mycelia were taken from the transition stage between the middle and late exponential growth phase. The optimum sucrose concentration for stability of protoplasts was 0.3∼0.5M. Reversion of protoplasts to a filamentous state was not occur on R_1, R_2, R_3 regeneration media by direct plating method, but 0.1% regeneration frequency was obtained by top agar overlay plating method on R_3 media.

Purification and Properties of a Membrane-bound Alcohol Dehydrogenase from Acetobacter sp. HA

유진철,심정보,김형근,전홍성,김성준,Yoo, Jin-Cheol,Sim, Jung-Bo,Kim, Heung-Keun,Chun, Hong-Sung,Kim, Sung-Jin The Microbiological Society of Korea 1994 미생물학회지 Vol.32 No.1

초산 생성 균주인 Acetobacter sp. HA로부터 membrane-bound alcohol dehydrogenase(ADH)를 분리 정제하였다. 세포막 분획과 세포막에서 효소의 용출 그리고 크로마토그라피 방법 등을 적용하여 수율 35%, 153배 정제된 효소를 획득하였다. 정제된 효소의 분자량은 330,000 dalton이었으며, 각각 분자량 79,000과 49,000 그리고 45,000 dalton을 가진 세 종류의 subunit로 이루어져 있었다. 그리고 흡수 스펙트럼의 분석 결과 본 효소에는 cytochrome c가 존재함을 확인할 수 있었다. 본 효소는 메탄올을 제외한 1차 지방족 알코올을 기질로 이용할 수 있었으며, formaldehyde, acetaldehyde 그리고 glutaraldehyde의 경우에도 다소간 기질로 이용될 수 있었다. 본 효소의 에탄올에 대한 $K_m$값은 1.38mM이었으며, 최적 pH와 온도는 각각 5.0~6.0과 32${\circ}C$이었다. 본 효소의 활성은 $V_2O_5$와 $ZnCl_2,\; NiCl_2$같은 금속 이온에 의하여 저해되었다. Membrane-bound alcohol dehydrogenase(ADH) was purified to homogeneous state fron an acetic acid producing bacteria, Acetobacter sp. HA. The enzyme was purified about 153-fold with an overall yield of 35% from the crude cell extract by solubilization and extraction of the enzyme with Triton X-100 and subsequent fractions by column chromatography. Upon sodium dodecyl sulphate-PAGE, the enzyme showed the presence of three subunits with a molecular mass of 79,000 daltons, 49,000, and 45,000 daltons, respectively. Absorption oxidized aliphatic alcohols with a straight carbon chain except for methanol. Formaldehyde, acetaldehyde and glutaraldehyde were also oxidizable substrates. The apparent $K_m$ for ethanol was 1.38mM. The optimun pH and temperature were 5.0~6.0 and 32${\circ}C$, respectively. $V_2O_5$ and heavy metals such as $ZnCl_2\;and\; NiCl_2$ were inhibitory to the enzyme activity.

토양으로 부터 분리한 방선균 KYR 26균주가 생산하는 항생물질의 분리 정제

유진철,방희재,심정보,이은하,김형근,김성준,김영호,이정준 조선대학교 부설 유전생물공학연구소 1993 유전생물공학연구지 Vol.3 No.-

1. 작용 범위가 넓고 부작용이 적은 의약용 항생물질을 screening하기 위한 목적으로 전남 지역 토양시료로 부터 방선균을 분리하여 항균활성이 우수한 방선균을 선별하여 KYR 26이라 명명 하였다. 2. KYR 26균주의 최적 배양배지는 Modified SMM배지 이었으며, 항생물질 생산은 배양 3일째에 최대의 수율을 나타내었다. 3. KYR 26균주가 생산하는 항생 물질은 Ion exchange chromatography, Adsorption chromatography, LH-20 coluom chromatography, Precipitation과 Silica column chromatography 과정을 통하여 항생물질 A, B를 분리정제 하였다. 4. 분리 정제된 항생 물질 A와 B는 TLC에서 각각 Rf치 0.53, 0.48에서 단일 spot로 나타났고,HPLC에서 항생물질 A와B는 각각 2.94, 7.66의 retention time을 보였으며 모두 98%의 순도를 보였다. 5. 분리 정제된 항생 물질 A와 B의 UV spectrum을 조사한 결과 항생물질 A,B는 모두 270nm에서 최대흡수 파장을 나타내었다. 현재 최종 분리 정재된 항생물질 A와 B의 구조 규명을 위하여 IR, ^1H-NMR, ^3C-NMR, Mass spectroscopy에 관한 실험이 진행중에 있다. Soil Actinomycetes were isolated from soil samples which were collected from the 100 sites of Cheon-nam areas. Isolated strains were tested for the production of antibiotics in various fermentation media using 4 strains of antibiotic sensitive test microorganisms. One strain which has excellent antibacterial activities was selected, and named to KYR 26. From culture broth of KYR 26 strain, two antibiotics were obtained through Ion Exchange Chromatography, Adsorption Chromatography, Gel Filtration Chromatography, Silica gel column chromatography and Crystalization. The finally purified two antibiotics exhibited single spot on the TLC chromatogram,and about 98 % purity on the profile of HPLC Chromatogram. Two antibiotics exhibited Rf 0.53 and 0.48 each on TLC with developing solvent system (MeOH:CHCl_3). The UV spectrum of isolated antibiotics exhibited a characteristic absorption maximum at 270nm.