D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

보디빌딩 선수들의 시합 전·후 신체조성과 체력 및 혈관탄성도 변화와 영양섭취 실태연구

곽재준,박재성,하수민,백영호 釜山大學校 附設 體育科學硏究所 2013 體育科學硏究所 論文集 Vol.30 No.-

The purpose of this study were to investigate a study of body composition, physical fitness, change of vascular compliance and nutrient intake status a before and after bodybuilders match. In this study, participants were 6 bodybuilders. The analyzed data brought about the following results by using paired t-test with SPSS 20.0. The results of the research were as follows. In to body weight and BMI were significantly increased but sit and reach were significantly decreased after match. There was non significantly difference in vascular compliance. Calories, lipids, carbohydrates, fiber and ash had significantly increased after match than before match. Calcium, iron, sodium, potassium, vitamin A, retinol, vitamin B1, vitamin B6, niacin, vitamin C, vitamin E had significantly increased after match than before match. Therefore, Bodybuilders need correct regular diet habit and exercise prescription after match. 본 연구는 B광역시 남자 보디빌딩 선수 6명을 대상으로 신체조성, 체력, 혈관탄성도 및 영양소 섭취상태를 시합 전·후를 분석하는데 목적이 있다. 자료처리는 SPSS 20.0 프로그램을 이용하여 paired t-test를 실시하였고, 연구결과에서 다음과 같은 결론을 얻었다. 시합전에 비해 시합후에 체중과 BMI는 유의하게 증가하였고, 유연성은 유의하게 감소하였고, 혈관탄성도는 유의한 변화가 없었다. 그리고 열량, 지질, 당질, 식이섬유 및 회분은 시합 전 보다 시합 후 유의하게 증가하였다. 또한, 칼슘, 철분, 나트륨, 칼륨, 비타민 A, 레티놀, 비타민 B1, 비타민 B6, 나이아신, 비타민 C, 비타민 E는 시합 전 보다 시합 후 유의하게 증가하였다. 이상을 종합하여 볼 때, 시합 전보다 시합 후에 영양소의 섭취증가로 인해 체중이 증가하는 것을 알 수 있으며, 이상의 연구 결과에서 시합전, 후의 식습관, 맞춤형 운동처방 및 체계적인 컨디셔닝 관리가 요구된다.

거대적아구성빈혈의 임상적 고찰

곽승근,천재민,박남숙,박상은,박수진,윤환중,김삼용,조덕연 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Megaloblastic anemias are disorders caused by impaired DNA synthesis and chara-cterized by the presence of megaloblastic cells. Clinical data on these disorders in Korea are limited. The aim of this study was to analyze clinical characteristics of megaloblastic anemias caused by vitamin B12 deficiency. We identified 40 cases who were diagnosed as megaloblastic anemia from January 1993 to December 2002 in a retrospective study on the basis of chart review. We investigated the annual distribution of diagnosed patients, clinical manifestations and response to treatment. 19 cases were complicated with a previous total gastrectomy(gastrectomy group) and 21 cases had no history of surgical operation(non-gastrectomy group). The most common presenting complaint was generalized weakness. The median age at diagnosis was 7(range: 38-79) years in non-gastrectomy group. The mean pos-toperative duration was 7(range: 4-25)years in group of total gastrectomy and mean level of serum vitamin B12 was lower than 100 pg/mL in both group. Peripheral blood revealed thrombocy-topenia, macrocytic anemia and hypersegmented neutrophil in all cases. After treatment of pareliteral vitamin B12, Their clinical symptoms and hematologic findings improved except two patients who had bone marrow with lower cellularity. Cobalamin replacement after total gastrectomy is still missing. Diagnoses are made too late in a population of patients, resulting in irreversible neurological sequale, and we suggest that Pernicious anemia seems not to be very rare in Korea.

脂肪組織에 있어서 Glucose-〔U〕-^14C의 脂質에의 編入에 대한 人蔘成分의 影響

郭再濚,曺準承 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

攝食 및 絶食狀態에서 또는 여기에 人蔘을 投與함으로써 脂肪組織에 있어서 glucose의 脂質生合成에의 利用이 어떻게 變動하는 가를 觀察하기 위하여 白蓼根의 methanol 抽出成分을 分離 乾燥하고 이것을 體重 100g當 5㎎ 을 生理的食監水 0.5㎖에 녹여서 쥐에게 4日間 注入하고 對照群에는 같은 量의 食監水만 投與해서 다시 이것들을 正常 攝食群과 24時間 絶食群으로 各各 나누어서 人蓼抽出物 또는 生理的食監水를 마지막 投與한 한 時間 뒤에 體重 100g當 glucose-(U)-^14C 4μC와 carrier로써 D-glucose 100㎎를 法入하고 3時間後에 腹腔內의 脂肪組織을 摘出하여 이의 脂質量과 이에 編入된 glucose의 放射性 炭素量을 測定하였다. 脂肪組織中의 脂質含量은 人蓼投與로써 攝食時에는 뚜렷한 增減이 없었으나 絶食時에 있어서 그 減少를 적게 하였다. 投與한 放射性 glucose는 脂肪組中의 脂質에 활발히 編入되었으며 또한 이것은 絶食狀態나 人蓼投與로써 變動하였다. 卽 放財性 glucose의 脂質에의 編入率은 攝食群에 비하여 絶食群에서 約 1.9倍로 높았으며, 여기에 人蓼을 投與한 攝食群에는 多少 增加의는 傾向이었으나 絶食群에서는 約 1.3倍로 若干 높았었다. 그러나 人蓼을 投與한 編入率은 人蓼을 投興하지 않는 絶食群의 그것에 比하여 約 3分의 1이나 낮은 값이었다. 脂肪組織에서의 이러한 현상들은 血淸脂質에 대해서도 유사한 경향을 나타내었다. To investigate effects of ginseng extract on the incorpcration of glucose-(U)-^14C into lipid of adipose tissue in rats, a methanol extract of the roots of dried ginseng was prepared. The extract was injected into the peritoneal cavity of rats in single daily dose of 5㎎ in 0.5㎖ of saline solution per body weight of 100gm for 4 days, and the control group was injected with 0.5㎖ of saline. The two groups were divided into the feeding and the fasting (24 hours). After one hour following the final administration of ginseng extract and saline respectively, all rats were injected with 4μCi of glucose-(U)-^14C mixed with 100gm of glucose, per body weight of 100gm. Three hours later, the amount of lipid content was checked, and the radioactivity of lipid ^14C was measure with gas flow counter. The lipid content of adipose tissue was not so notable increasing effect in the normal feeding with ginseng. However, under the fasting group, the decreasing rate of lipid content was minimized with ginseng administration. The utilization rate of glucose-(U)-^14C into lipid of adipose tissue showed a slight increase under a normal feeding with ginseng. However, it decreased under fasting with ginseng administration. These effects of ginseng administration in the adipose tissue appeared almost same effects in the rat serum.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

이동 애드 혹 네트워크에서의 환경적응형 QoS AODV 라우팅 프로토콜에 관한 연구

裵振勝,宋東勳,吳世德,鄭燦赫,金玄郁,李光培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we propose a bidirectional mobile Ad Hoc routing protocol based on AODV(ad-hoc on-demand distance vector routing) with QoS(quality of service) support. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS guarantee between end points is a very important issue. The existing AODV routing protocol is implemented to use only BE (Best Effort) service. However, actual wireless mobile environment requires to reduce a waste of communication resources and meet with the real-time change of data paths by setting up routes with QoS support, considering available communication resources at each node in advance. Therefore, in this thesis, we propose an adaptive QoS support method using hello message in order to rapidly meet with the change of available communication resources and to smoothly perform route maintenance. For performance evaluation, we analyzed the average data reception rate. At that time, we used evaluation parameters such as node's mobility-stop time, hello message period, and packet priority, considering the node's mobility and the number of best effort/QoS data flows. As results, we found that with our proposed method the average data reception rate was increased 16.293%, 15.914%, 17.238% for 300 mobility-stop second case and 4.809%, 4.264%, 2.956% for 300 mobility-stop second case.

조류와 유기화합물의 동시제거를 위한 흡착 - DAF 복합공정

이재욱,곽동희,최승필,정흥조 대한상하수도학회 2004 상하수도학회지 Vol.18 No.2

Dissolved air flotation (DAF) is an effective solid/liquid separation process for low density floc particles such as algal, color-alum and clay-alum flocs produced from low turbidity water. The removal of taste and odorcausing organics (2-mthylisoboneol and geosmin) originating from algae in drinking water is a local and worldwide concern. Although DAF has been effectively applied for the removal of suspended solid, its application for the treatment of dissolved organic carbon is very limited. In this study, a new hybrid system consisting of adsorption and DAF processes was introduced for the simultaneous removal of algae and taste and odorcausing organics. Powdered activated carbon (PAC) was used as an adsorbent. In this proposed system, the major concern of eliminating the spent PAC from the system was also addressed. It was found that zeta potential of algae and PAC was increased with coagulant dosage, and the removal efficiency in DAF was also enhanced up to 90-95% under the given experimental conditions. Based on this study, the hybrid process was found to be a promising technology for the simultaneous removal of algae and dissolved organic pollutants.

동일한 국제예후지표(International Prognostic Index)를 나타내는 미만성 대형 B-세포 림프종에서 Bcl-2와 p16의 임상적 중요성

박상은,박수진,곽승근,박남숙,천재민,윤환중,조덕연,김삼용,김진만 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

In Korea, malignant lymphoma is a common cancer, comprising about 2.7% of all malignant neoplasm. Diffuse large B cell lymphoma is the most common lymphoma, representing about 50% of all Non-Hodgkin's lymphoma. Diffuse large B-cell lymphoma is usually considered as heterogeneous group of neoplasms rather than a single clinicopathological entity. Clinical prognostic systems, including the International Prognostic Index (IPI), although useful to assess overall prognosis, embrace patients with heterogeneous prognoses. But International Prognostic Index scoring system is not sufficiently predict the prognosis. It is likely that the prognostic assessment of patients with diffuse large B-cell lymphomamight be improved by using biological features. Bcl-2 protein and p16 protein expression is recognized as useful biologic markers predicting the prognosis of patients with diffuse large B-cell lymphoma. To determine the clinical significance and prognostic value of bcl-2 and p16 proteins expression patterns, we studied 18 patients with de novo DLBL, whose archival pathology specimen were available for immunohistochemistry studies, atChungnam National University Hospital from September 1992 to December 2000. Archival specimens from each patient were immunostained with respective antibodies for bcl-2, p16. The results are as follows; 1) The median age was 54(rage : 37-69). There were 12 male patients(66.7%) and 6 female patients(33.3%) The 'B' symptom was abscentin all patients. The stages were as follows : Ⅰ, 2 patient(11.1%), Ⅱ, 10 patient(55.6%), Ⅲ, 4patient(22.2%) and Ⅳ, 2patient(11.1%). 3 patients(16.8%) had the elevated LDH level, 14 patients(77.8%) had the normal LDH level and 1 patients(5.6%) was not identified the LDH level. 2 patients(11.1%) had the bulky disease and 16 patients(88.7%) had no bulky diease. The distribution of ECOG status were O, 2 patients(11.1%c), 1, 14patients(77.8%) and 2, 2patients(11.1%). 2) Theimmunohistochemistry results are as follows bcl-2:+,10 patients(55.6%), bcl-2:-, 8patinets(44.4%), p16:+,3 patients(16.7%), p16:-, 15patients(83.3%) 3) After a median follow UP durations of 67 months, the median survival time was 57 months with a rage of 7-100+ months. 5-years overall survival rates was 44% by Kaplan-Meier method. 4) Reduced overall survival was demonstrated in the patients who expressed bcl-2 protein(P=0.0174). 5-year overall survivial rate was 12%(bcl-2 expression) versus 88%(no bcl-2 expression) 5) Among diffuse large B-cell lymphoma patients with IPI score 0-1, reduced overall survival was demonstrated with bcl-2 expression(P=0.023). 5-year overall survival rate was 18%(bcl-2 expression group) versus 100%(no bcl-2 expressiongroup) 6) Median survival durtation of diffuse large B-cell lymphoma patients negative for p16 expression was 57 months whereas p16 postive patients' median survival duration was not reached(P=0.4478). In diffuse large B-cell lymphoma patients with identical IPI scores, bcl-2 expression had additional prognostic value.

광주광역시 치매의 유병률과 위험인자의 도시-농촌 지역 비교

신일선,김재민,윤진상,김석재,양수진,김웅장,이승현,강순아,곽지영,이형영 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.6

연구목적 : 본 연구의 목적은 광주광역시의 도시 및 농촌 지역에 거주하는 노인에서 치매의 유병률과 위험인자를 조사하고자 하였다. 부가적으로 이 두 지역간에 치매의 유병률과 위험인자를 비교하고자 하였다. 방 법 : 광주광역시의 전형적인 도시와 농촌지역의 65세 이상의 노인(N=1,598)을 대상으로, 2단계의 역학연구를 수행하였다. 1차 선별 단계에서는 38명의 훈련된 연구보조원이 대상노인을 면담하여, 사회인구학적 특징을 조사하고 한국판 간이정신상태검사(Korean version of Mini-Mental State Examination ; MMSE-K)로 평가하였다. 2차 임상 진단 단계에서는 1차 단계에서 MMSE-K 점수가 24점 이하인 노인(N=431)을 대상으로, 전문연구팀의 임상적 평가를 통해 치매를 진단하고 원인질환별로 알쯔하이머병(Alzheimer's disease ; AD), 혈관성 치매(vascular dementia ; VaD) 및 기타 치매로 분류하였다. 대상노인에서 치매의 유병률을 측정한 후, 광주광역시 전체 노인의 연령보정 유병률을 산출하였다. 전체 대상에서 AD의 위험인자를 조사한 후, 도시와 농촌군으로 나누어 분석을 다시 시행하였다. 결 과 : 1차 및 2차 단계의 참가율은 각각 71.0%(N=1,134) 및 83.2%(N=410) 였다. 연구 참여 노인(N=1,134)에서 전체 치매, AD 및 VaD의 유병률은 최소 각각 9.7%, 5.2%, 1.8%였다. 광주광역시 전체 노인의 연령 보정 유병률은 각각 10.7%, 5.7%, 1.9%인 것으로 산출되었다. AD의 독립 위험인자는 고령, 여성, 무학이었다. 도시와 농촌에 따른 유병률의 차이는 파악되지 않았다. 그러나 AD의 위험인자는 지역에 따라 차이가 있었다. 도시 노인에게는 무학만이, 농촌 노인에게는 고령과 여성인 경우가 독립 위험인자였다. 결 론 : 광주광역시 노인에서 치매의 유병률은 높았고, 이는 국내 다른 지역에서 수행된 선행연구 결과와 유사하였다. 알쯔하이머형 치매의 위험인자는 도시와 농촌에 따라 다른 결과를 보였다. 본 연구 결과는 한국에서의 치매의 실태를 반영하며, 치매의 적절한 관리와 예방을 위해서는 지역성 특성이 고려되어야 함을 시사한다. Objectives : The purpose of this study was to investigate the prevalence rate and risk factors of dementia in an urban and a rural sample of older persons in the metropolitan Kwangju area, Republic of Korea. In addition, the prevalence rate and risk factors of dementia between the two samples were compared. Methods : A two-phase epidemiological study of residents aged 65 or over(N=1,598) was conducted in a highly developed, urban and a non-contiguous, poorly developed, rural area of metropolitan Kwangju. In the first(screening) phase, 38 trained research assistants collectively peformed in-home interviews for all participants. Detailed sociodemographic characteristics were gathered and the Korean version of Mini-Mental State Examination (MMSE-K) was administered. In the second (diagnosing) phase, diagnosis of dementia was established for those scoring 24 or less on the MMSE-K in the screening phase(N=431), and etiological types of dementia were classified to Alzheimer's disease(AD), vascular dementia(VaD) or miscellanous type by two teams of experts. Prevalence rate of dementia was determined in the participants, and age-standardized prevalence rate adjusted by age distribution in metropolitan Kwangju was estimated. For AD, the risk factors were investigated in all participants, and analyses were repeated for the urban and rural samples separately. Results : The participating rates in the first and second phases were 71.0%(N=1,134) and 83.2%(N=410), respectively. In the participants, the prevalence rates of dementia(all types), AD and VaD were 9.7%, 5.2% and 1.8%, respectively. The age-standardized prevalence rates were 10.7%, 5.7% and 1.9%, respectively. Aging, female gender and no education(complete lack of formal education) were identified as independent risk factors for AD. No difference between the urban and rural samples was observed in the prevalence rates of dementia(all types), AD and YaD. However, risk factors for AD differed between these areas. In the urban sample, no education was the only significant factor ; whereas, aging and female gender were identified as risk factors in the rural sample. Conclusion : A high prevalence rate of dementia was observed among old persons in metropolitan Kwangju, consistent with previous studies conducted in other Korean areas. The risk factor profi1es for AD differed between urban and rural settings. The results of this study reflect the current status of dementia in Korea and suggests that appropriate management and prevention strategies for dementia should be tailored toward the geographic settings.

원추형 회전체-자기베어링의 능동 진동 제어

석창욱,양승준,곽병철,이재응 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

기존 접촉식 베어링과 비교할 때 능동 자기 베어링(AMB)는 많은 장점을 가진다. 자기 베어링 시스템은 주축을 자기력을 이용하여 부상시켜 기계적인 마찰이 없는 상태에서 회전시키기 때문에 윤활을 하지않고도 작동시킬 수 있다. 따라서 이 베어링은 우주에서의 진공상태와 같은 극한상황에서도 사용할 수 있다. 특히 원추형 자기 베어링은 4조의 전자석으로 구성되어 래디얼 타입 자기 베어링보다 소형, 경량화할 수 있는 장점을 가진다. 본 논문에서는 먼저 원추형 자기베어링에 대한 운동방정식을 유도하고, 이 시스템의 진동 제어를 위해 비연성인 디지털 PD, PID 제어기를 설계하였다. 컴퓨터 모의실험을 통해 제어기의 성능을 평가하여 만족할 만한 성능을 얻었다. There are many advantages of the Active Magnetic Bearing(AMB) system, compared with conventional contacting bearing systems. Since the spindle in the AMB system is levitated by electro-magnetic forces and rotates without mechanical friction, it can run without lubrication. So, it can run in the extreme circumstances, e.g. a vacuum condition in the space. Particularly, the cone-type AMB system consists of only 4 couples of magnet, it can be smaller and lighter than radial-type AMB system. In this thesis, the equations of motion of the cone-type AMB system is derived first, and the digital PD and PID controllers are designed for the vibration control of the system. The controllers are designed based on the decoupled equations of motion, and the performance of the controllers are evaluated through the computer simulations. The results of the simulation show good performance of the controllers.