經口的長期鐵投與에 依한 各臟器鐵의 變動에 關하여

曺準承,白義兌 최신의학사 1964 最新醫學 Vol.7 No.10

알코올의 代謝 및 作用

曺準承 대구보건대학 1974 대구보건대학 論文集 Vol.2 No.1

食餌性 指肪의 含量과 種類가 흰쥐 Lipoprotein의 分劃像에 미치는 影響

李宇炯,郭春植,曺準承 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.1

食餌脂肪의 含量 및 그 種類가 血淸 lipoprotein 分劃에 어떠한 影響을 미치는가를 調査하기 위하여 離乳된 쥐를 10마리씩 3群으로 나누어서 12% calorie 脂肪食餌를 對照로 하여 植物性高脂肪食餌로서 45% calorie corn oil 食餌와 動物性高脂肪食餌로서 45% calorie butter 食餌를 各各 12週間 投與한 後에 各群의 血淸 lipoprotein 分劃을 測定하여 다음과 같은 結果를 얻었다. 對照인 正常食餌群의 血淸 chylomicron, VLDL, LDL 및 HDL 値는 各各 36.4±10.7㎎/㎗, 15.9±5.2㎎/㎗, 26.2±4.7㎎/㎗ 및 93.3±18,7㎎/㎗이었다. 對照群과 比較해서 45% corn oil 投與群의 血淸 lipoprotein 分劃値는 別로 差異를 나타내지 않았으나 45% butter 投與群에서는 chylomicron, VLDL 및 LDL 値는 모두 1.5倍 以上 높았으며 反對로 HDL 値는 13%가량 떨어지는 傾向을 나타내었다. 以上의 結果로 보아 대부분의 不飽和脂肪酸으로 構成된 脂肪食餌를 投與했을 때는 LDL 以外는 別로 變化가 일어나지 않으나 飽和脂肪酸이 많은 動物性脂肪을 多量攝取하면 血淸 lipoprotein의 모든 分劃像에 變化를 招來한다고 할 수 있다. Effect of a high level and types of dietary fat on serum lipoproteins was investigated in Sprague-Dawley rats weighing 60-65gm. The animals were divided into three groups and were fed 3 different fat diet: 12% calorie fat diet, 45% calorie-butter fat diet, and 45% calorie-corn oil diet. The diets were isoenergetic (4.4Kcal/gm), and were fed to the rats ad libitum for 12 weeks. Mean serum lipoprotein levels in mg/dl for the control group were 36.4±10.7: chylomicron, 15.9±5.2: VLDL, 26.2±4.7: LDL, and 93.3±18.7: HDL. The high corn oil diet did not significantly change the levels of serum lipoproteins except 1.5-fold increase in LDL. However, the high-butter fat diet showed 1.5-to 2-fold increase in serum levels of chylomicron. VLDL and LDL, but no change in HDL level. These result indicate that long-term feeding fo high-fat diet composed of primarily saturated fatty acid increases serum levels of lower dinsity fractions of lipoprotein.

血淸 Alanine Aminotransferase와 Alkaline Phosphatase의 活性에 대한 饑餓 및 人蔘의 效果

邊永宙,郭春植,曺準承 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.1

絶食시켰을 때 肝機能檢査時에 많이 사용되는 血淸 alanine aminotransferase와 alkaline phosphatase거 어떻게 變化하며 또 人蔘의 投與가 이것에 어떠한 效果를 미치는가를 알아 보기 위하여 體重이 約 250g되는 Sprague-Dawley種의 흰쥐를 使用하여 調査해 본 結果는 다음과 같았다. 動物을 絶食시키므로써 體重은 1日에 約 10%씩 減少되었으며, 人蔘을 投與해도 絶食으로 因한 體重減少에는 別다른 영향을 미치지 않았다. 血淸 alanine aminotransferase의 活性은 絶食後 減少되어 絶食 3日째에는 約 5分의 2로 顯著히 낮은 値를 보였다. 人蔘의 投與는 絶食으로 因한 이 酵素活性의 低下를 뚜렷하게 沮止하는 效果를 나타내었다. 한편 血淸 alkaline phosphatase는 絶食시켜도 別다른 變動을 나타내지 않았으며 따라서 人蔘은 이 酵素의 活性에는 영향을 미치질 않았다. Changes in serum alanine aminotransferase and alkaline phosphatase activities were studied in Sprague-Dawley rats by starvation and ginseng. In starved rats, body weight were decreased in rate by 10% a day and weight decrease was not affected by ginseng administration. Serum alanine aminotransferase activities were decreased by starvation and revealed 60% decrease on the third day of starvation. Ginseng administration significantly inhibited this decrease of enzyme activity produced by starvation, while serum alkaline phosphatase revealed no noticeable changes by starvation, and ginseng administration.

韓國兒童의 血淸鐵 및 鐵結合能에 關하여

曺準承,蔣貞仙,洪大一 대구보건대학 1977 대구보건대학 論文集 Vol.3 No.-

韓國人 兒童(6才∼11才)의 血淸鐵, 不飽和鐵結合(UIBC) 및 總鐵結合能(TIBC)의 正常値를 調査하기 위하여 男兒34名과 女兒 34名을 對象으로하여 檢査한 結果는 다음과 같았다. 血淸鐵 : 男兒 135±30 (74-196)㎍/100㎖ 女兒115±34 (47-183)㎍/100㎖ UIBC 男兒 237±41 (155-319)㎍/100㎖ 女兒 225±46 (133±317)㎍/100㎖ TIBC : 男兒 366±32 (302-430)㎍/100㎖ 女兒 330±41 (248-412)㎍/100㎖ %Saturation : 男兒 37.1±8.0(21-53)% 女兒 34.6±11.9(21-59)% 以上의 値들은 成人의 그것과 比較해서 別 差異가 없었다. 그리고 大體的으로 女兒의 값이 男兒의 그것 보다 떨어지는 結果로 보였다. This paper has been caried out to determine the normal values for serum iron and iron binding capacity of Korean children, and to compare with that of adult men Non-festing blood specimens were collected from 34 males and 34 females in the morning (am. 9-10). Serum iron was estimated by the modified method of Barkan-Walker's, and the total iron binding capacity by Ramsay method. The results obtained were as follows: Serum iron: male 135-30ug 100ml (range; 74-196) female 115-34ug 100ml (range; 47-183) U.I.B.C. : male 237-41ug 100ml (range; 155-319) female 225-46ug 100ml (range; 133-317) T.I.B.C. : male 366-32ug 100ml (range; 302-412) female 330-41ug 100ml (range; 248-412) % Saturation:male 37.1-8.0% (range;21-53) gemale 34.6-11.9%(range;21-59) These normal values of children were no significant difference between that of adults which are reported by other investigators.

흰쥐의 간섬유화 과정에서의 교원질의 다형성 및 양적 변동

조준승,류현모,은선진,김인산 ( Joon Seung Jo,Hyun Mo Ryoo,Sun Jin Eun,In San Kim ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

Macromolecular collagen components in rat liver at different stage of fibrosis induced by common bile duct ligation were studied. Animals were killed on the 3rd, 7th, 14th, 21st and 29th day after bile duct ligation and liver specimens and blood samples were obtained. Hepatic collagen was fractionated into neutral salt soluble, acid soluble, pepsin soluble and insoluble fractions, and hydroxyproline content of each fraction was determined. The collagen content, especially in insoluble fraction, was increased as the duration of biliary obstruction increased. Pepsin solubilized collagens were subjected to determination of the collagen types by SDS-polyacrylamide disc gel electrophoresis. The ratio of type I to type III collagen was decreased gradually with progression of fibrosis. These results indicate that an alteration in tissue collagen polymorphism as well as variations in the collagen solubility accompany the fibrotic process, suggesting possible pathogenetic implication.

인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영 ( Joon Seung Jo,Tai Ho Chung,Chun Sik Kwak,Choong Young Kim ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

Cultivated dry ginseng root was pulverized and extracted with methanol. Theextractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200∼2208 were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5㎎ per 1008 body weight of the ginseng extract in 0.5㎖ of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 μc of glucose-^(14)C with carrier 100㎎ glucose per 1008 of body weight or 4 μc of amino acid mixed-^(14)C with carrier 20㎎ L-alanine per 1008 of body weight were injected peritoneally for the correspond group and control.. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase. To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately alter the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-^(14)C or amino acid mixed-^(14)C in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the administration of ginseng both the time of feeding and fasting.

인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영,Jo, Joon-Seung,Chung, Tai-Ho,Kwak, Chun-Sik,Kim, Choong-Young 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1

인삼 성분을 메탄올로써 추출하고 이것이 쥐에 있어서 당질의 산화와 당의 생성 즉 glycogenesis 및 glyconeogenesis 그리고 당 신생반응의 첫 단계 반응을 조절하는 효소라고 믿고 있는 간의 phosphoenolpyruvate carboxylkinase의 활성에 어떠한 영향을 미칠것인가를 조사하여 보았다. 인삼 추출물을 쥐 체중 100g당 5mg이 되도록 생리적 식염수 0.5ml에 녹여서 1일 1회 4일간 복강내로 미리 투여하였으며 대조군에는 같은 양의 식염수만 주입하였다. 이것을 다시 계속 섭식시킨 군과 24시간 굶긴 군으로 나누어서 인삼 추출물을 투여한 1시간뒤에 glucose-$^{14}C$ 또는 amino acid mixed-$^{14}C$를 주입하여 호기중애 배출되는 $^{14}CO_2$ 양을 검출하고 또 3시간 후에 간속의 glycogen과 phospho-enolpyruvate caboxykinase에 대해서 검토하였다. 이 실험 결과 인삼은 glucose의 산화를 현저히 증가시켰으며 이 효과는 기아상태에서 더욱 촉진적으로 작용하였다. 그러나 인삼은 간에서의 glycogenesis나 glyconeogenesis에 대해서는 효능이 그리 없는것 같았다. 즉 인삼투여군은 대조군에 비하여 간의 glycogen농도나 $^{14}C$ 화합물의 glycogen에의 편입속도가 다같이 별 차이를 나타내지 않았다. 그리고 간의 phosphoenolpyruvate carboxykinase의 활성에도 인삼의 별 영향을 미치지 않았다. Cultivated dry ginseng root was pulverized and extracted with methanol. The extractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200~220g were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5mg per 100g body weight of the ginseng extract in 0.5ml of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 uc of glucose-$^{14}C$ with carrier 100mg glucose per 100g of body weight or $4\;{\mu}c$ of amino acid mixed-$^{14}C$ with carrier 20mg L-alanine per 100g of body weight were injected peritoneally for the correspond group and control. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase, To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately after the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-$^{14}C$ or amino acid mixed-$^{14}C$ in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the adminisration of ginseng both the time of feeding and fasting.

成長期 家兎 肝臟中 貯藏鐵의 量的變動에 關하여

曺準承,鄭熙坤,黃一愚,金源准 慶北大學校 醫科大學 1967 慶北醫大雜誌 Vol.8 No.1

白鼠에 있어서 Alpha aminoisobutyric acid C^(-14)의 各組織細胞內移行에 對한 蛋白良의 影響

曺準承 慶北大學校 醫科大學 1967 慶北醫大雜誌 Vol.8 No.2