Repellent efficacy of essential oils and plant extracts against Tribolium castaneum and Plodia interpunctella

Lee Hwa‐Eun,Hong Su Jung,Hasan Najmul,Baek Eun Joo,Kim Jun Tae,Kim Young‐Duk,Park Mi‐Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.9

This study was conducted to investigate the repellent efficacy of essential oils (Origanum vulgare, Pimpinella anisum, and Tanacetum cinerariifolium) and four plant extracts (Agastache rugosa, Capsicum annuum, Citrus reticulata, and Ginkgo biloba) against Tribolium castaneum (adults and larvae) and Plodia interpunctella (larvae). Gas chromatography/mass spectrometry analysis revealed the presence of carvacrol, anethole, and jasmolin I as the predominant constituent in O. vulgare, P. anisum, and T. cinerariifolium, respectively. Furthermore, ethyl hexopyranoside, 9,12‐octadecadienoic acid, cyclopentanol, and 2‐cresol were identified in A. rugosa, C. annuum, C. reticulata, and G. biloba, respectively. The repellent efficacy of each essential oil, plant extract, and the combination of oils was evaluated using a specially designed cylinder trap for 120 h. Among the three oils, O. vulgare and T. cinerariifolium had greatest repellent efficacy against P. interpunctella larvae. T. cinerariifolium exhibited effective repellence against the adults and larvae of T. castaneum. Therefore, O. vulgare (O) and T. cinerariifolium (T) were selected for further investigation of combined effects. Two essential oils were mixed in three different ratios of OT1 (1:3), OT2 (1:1), and OT3 (3:1). The repellent efficacies of OT1 and OT2 against the adults of T. castaneum were significantly greater than that of OT3. OT1 was effective against the larvae of T. castaneum, whereas OT2 was effective against the larvae of P. interpunctella. OT1 enhanced the repellent efficacy by approximately five times against larvae of T. castaneum, compared with that of T. cinerariifolium. Overall, OT1 was selected as the best repellent substance against all the tested insects.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

농작물 해충 및 진균류 방제를 위한 방선균의 분리 및 동정

이은정,강경돈,황교열,김두호,김신덕,성수일 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1

Twenty seven out of ca. 5,000 actinomycete strains, which were isolated from soil collected throughout the country, showed antimicrobial effects against fungi, Rhizopus stronifer (ATCC6227a), Rhizoctonia solani (KCCM 11271) and yeast, Candida albicans(ATCC10231). From these antifungal microorganisms, we further selected seven strains which seemed to produce insecticidal substances with in vivo test, using silkworm, Bombyx mori and beet armyworm, Spodoptera exigua. Morphological and biochemical experiments revealed that three strains out of seven were streptomyces. Further investigations on the physical and chemical properties of these antifungal and insecticidal substances are now in progress.

한국인에서 혈소판 당단백 Ⅱb/Ⅲa 유전자 다형성과 관동맥 성형술 후 재 협착과의 관계

이민수,이정우,김보영,임대승,강정아,김정희,김윤철,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Platelet aggregation is the final pathway of acute coronary syndrome such as acute myocardial infarction and unstable angina. Platelet glycoprotein IIb/IIIa is a membrane receptor for fibrinogen and yon Willebrand factor and it plays an important role in platelet aggregation and in the pathogenesis of acute coronary syndrome. It is known that polymorphism of the gene that encoding platelet glycoprotein IIb/IIIa(PI^A1/A2) is strongly related to acute coronary syndrome in Caucasian, but not in Koreans. We investigated relationship between platelet glycoprotein llb/Illa gene polymorphism and restenosis of coronary artery after angioplasty in Koreans. Total 371 patients(M=251. F=120) were enrolled. Angioplasty group comprised 143 patients who underwent coronary angioplasty, and in the angioplasty group, restenosis group comprised with the 65 patients who had restenotic lesion over 50% of luminal diameter in follow-up coronary angiography. Normal group comprised 153 patients who had no significant angiographic lesion and variant angina group comprised 75 patients who were positive in ergonovine test. Genomic DNA was extracted from peripheral arterial blood. To determine the frequency of P1^A1/A2 genotype, polymerase chain reaction(PCR) was done and the product was restricted with Mspl. 3%. agarrose gel electrophoresis showed restriction fragment length polymorphism. Clinical profile and risk factor were also reviewed. Among all 371 patients of study group, genotype of only one patients in restenosis group if is proven to be PI^A1/A2 heterozygote. All patients of normal study group, no restenosis group, and the other patients in restenosis group have an PI^A1 homozygote genotype. In our study, platelet glycoprotein IIb/Illa polymorphism has no relationship with restenosis of the coronary artery after angioplasty in Koreans. But the genotypic frequency of platelet glycoprotein IIb/IIIa gene polymorphism in Koreans is concordant with that of previous studies.

고정화 Photobacterium phosphoreum과 휘발성 독성 물질과의 반응성

이은수,전억한 경희대학교 생명자원과학연구원 1999 硏究論文集 Vol.20 No.-

휘발성 독성 물질 측정용 biosensor로서 P phosphoreum을 sodium alginate로 고정화하여 독성물질에 대한 반응성을 조사한 결과 각각의 독성물질마다 특이한 hnearity 관계식이 설정되는 농도 범위가 있었고 이를 이용하여 EC_50을 계산할 수 있었다 IC_50과 비교해보면 EC_50을 이용하는 것이 시간과 비용을 줄일 수 있고 낮은 농도의 독성 물질에 대해 더 민감한 반응을 나타내었다 특히, 휘발성이 강한 독성 물질 일수록 휘발성 방지를 위해서는 세포를 고정화하는 것이 더 효과적이었다 따라서 휘발성 독성물질에 대해서 free cell보다는 고정화한 세포가 더 민감하게 반응하였고 sodium alginate 고정화 세포보다는 strontium alginate로 고정화된 세포가 민감한 반응을 나타내었다 Immobilization method was investigated in order to maintain the stability of bioluminescence of Photobacterium for monitoring the volatile toxic substances. Sodium alginate was used as an immobilization matrix for P. phosphoreum. For the relationship between bioluminescene of immobilized P. phosphoreum and toxic substances, kinetic parameters of specific rate (μ), ratio of bioluminescence intensity (Io/I), and gamma (γ) value were estimated. The bioluminescence intensity was found proportional to the concentration of toxic substances and the free cells showed more sensitivity than immobilized cells when volatile substances were exposed to the cells. However, bioluminescence increased slightly with time because of volatility. Furthermore, P. phosphoreum immobilized on strontium alginate were rather favored in comparison with cells immobilized on sodium alginate for the response to substances used.

한국판 스미스클라인 비챰의 '삶의 질'척도를 이용한 공황장애 환자의 삶의 질에 관한 연구

이승호,제영묘,김상엽,이대수,최은영 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

Objectives : In this study, we researched the change of 'quality of life(QOL)' of patient with panic disorder by KvSBQOL and the predictors affecting the change of 'quality of life'. Methods : 30 outpatient with panic disorder were tested by questionnare for panic disorder and KvSBQOL. Frst, they were classified as when healthy before panic episodes(a point of time 1), when first diagnosed as panic disorder in the psychiatry(a point of time 2), and when treated after diagnosis(a point of time 3). Second, the change of QOL scores in five factors at each point was observed, Third, the correlation between the change of QOL scores(point 3 score-point 2 score) and the several predictors(Onset age, Degree of change in panic symtom, Degree of change in anticipatory anxiety, Degree of change in agraphobia, Spended time to diagnose panic disorder, Duration of treatment) were studied. Results : 1) QOL mean scores of a point of time 1,2 and 3 were 166.267±38.24, 94.367±34.00, 161.367±34.37, respectively. 2) The change of QOL scores in all five factors of KvSBQOL(factor1 Competence, factor2 Psychological well-being, factor3 Stability, factor4 Physical well-being, factor5 Activity)at each point was significant(P<.000). 3) The change of QOL mean scores(point 3 score-point 2 score) have significantly negative correlation with the change of score in anticipatory anxiety but not other predictors. Conclusions : Difference in QOL scores between a point of time 1 and 3 is 4.9, indicating that the psychiatric treatment is important in improvement of QOL, the anticipatory anxiety is an important predictor of QOL(P<.001). and it suggested that diminished anticipatory anxiety is very important to maintain of high QOL in patient with panic disorder.

폐굴껍질의 정석반응을 이용한 수중 인제거 특성

이호수,정유진,김은호,성낙창,이영형 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.2

The technology of removing phosphorous, considered as one of the most important control nutrients causing eutrophication in various water bodies, have been investigated by many researchers. Recently, phosphorous crystallization process is emerging as a new technology for phosphorous removal. In this study, waste oyster shells which can be easily obtained from the ocean, were used as a seed crystal, and their effects of several physical/chemical factors on the phosphorous removal efficiencies were examined by batch tests. Ca^(2+) and pH affected phosphorous crystallization process using waste oyster shells. As alkalinity of wastewater increased, phosphorous removal efficiencies gradually decreased. Phosphorous removal efficiencies were increased, as specific area and contact efficiency per unit area of waste oyster shells were increased. In case of high temperature, phosphorous crystallization process was rapidly advanced and phosphorous removal efficiencies were increased. Depending on X-ray diffraction analysis, it was showed that generation materials extracted from the surface of waste oyster shells with short reaction time were dominated by CaHPO₄ ·2H₂O, but progressed to Ca_(5)(OH)(PO₄)₃.

류마티스 관절염과 전신경화증의 중복증후군 1예

이은지,서광원,유충헌,박봉수,허민영,김지현,김혜란,김동욱 인제대학교 2008 仁濟醫學 Vol.29 No.-

Overlap syndrome is a disease that combines major features of more than one rheumatic disease in the same patient. A 58 year-old female who was diagnosed as rheumatoid arthritis (RA) developed skin thickening and dyspnea. Clinical and laboratory findings revealed that she was affected by systemic sclerosis (SSc) and interstitial lung disease. Finally, she was diagnosed as overlap syndrome consistent with RA combined with SSc. The authors report a case of RA-SSc overlap syndrome with relevant literatures.

독성 물질 측정을 위한 Photobacterium phosphoreum의 고정화에 관한 연구

이은수,이홍주,전억한 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

독성 물질 측정용 P phosphoreum의 고정화 물질 선택을위하여 고정화 방법을 크게 4가지로 나누어서 그 방법에 따라 각각 고정화 물질 한가지씩을 선택하여 P phosphoreum의 bioluminescence 안정성을 알아보았다. Polyacrylamide나 collagen에서는 bioluminescence가 유지를 못하고 20분 안에 급격히 떨어졌으나, alginate와 k-carrageenan은 온도를 높여야 gel이 형성되는 성질을 갖고 있기 때문에 저온성 발광 미생물인 P phosphoreum에 적합한 matrix가 되지 못한다. 따라서 P phosphoreum의 biluminescence를 안정되게 유지하면서 고정화가 용이한 polymer로는 alginate가 적합한 고정화 물질로 선정되었고 독성물질에 대한 P phosphoreum의 민감성을 bioluminescence의 변화로 살펴본 결과 각각의 독성 물질마다 특이한 linearity한 관계식이 설정되었고 EC_(50)를 쉽게 구할 수 있었다. The bacterial bioluminescence has been studied to detect toxic materials m foods and environment. A monitonng system can be developed with a fact that the bioluminescence is mhibited sensitively when the bioluminescent bacteria are exposed to toxic substances. However, a stability of bioluminescence must be improved for the purpose of developing continuous monitoring system. The mmobilization of Photobacterium phosphoreum, therefore, was investigated m view of long term of bioluminescence stability. Various matrixes, sodium algmate, k-carrageenan, collagen and polyacrylamide were studied for proper immobilization matnx of P. phosphoreum, in order to improve stability of bioluminescence. Collagen and polyacrylamide were shown to be madequate matenals for immobilization of P. phosphoreum since the bioluminescence decreased when cells were mixed with such matnx due to toxic effect of free radicals of matnx. In case of k-carrageenan, the biolumexence was stable compared with collagen and polyacrylamide. However, the k-carrageenan was not also a suitable matnx because cells were not able to mix with the matnx properly m low temperature at which gel is formed and, especially, cells of P. phosphoreum are psychrophilic luminescent bacteria. With a sodium algmate, the bioluminescence was stably maintained for 20 minutes and, in addition to that, a process of immobilization was simple for bioluminescent bacteria. Therefore, by using a sodium algmate as an mobduahon matrix, the relationship between biolummescence of immobilized P. phosphoreum and each toxic substance was shown linearity and could calculate EC_(50).