家蠶의 中腸變態에 關한 內分泌學籍 硏究

成洙一 한국잠사학회 1984 한국잠사곤충학회지 Vol.26 No.1

In order to investigate an endocrinological effect on metamorphosis of the midgut in silkworm, Bombyx mori, 20-hydroxyecdysone and crude extract of brain (PTTH) were injected into pupal isolated abdomens, devoid of prothoracic glands and also in the brainless diapausing pupae with the prothoracic glands (so-called dauer pupae). When ecdysone was assayed abdomens, drastic morphological changes occurred in the midgut. The wet weights of midguts were found decrease as like the normal pupae at metamorphosis. Proteineous changes in epithelial midgut were also observed; pupal specific proteins (PSP) vanished while the adult proteins were newly synthesized. Similar results were also found in the changes of protein in the midgut of dauer pupae by injecting ecdysone and pTTH.

동위효소 다형특성에 의한 누에 품종의 유연관계

성수일 수원대학교 기초과학연구소 1995 基礎科學論文集 Vol.4 No.-

Isoenzyme was used to characterize patterns of genetic relationships among 141 silkworms, Bombix mori, races in National Sericultural and Entomology Research Institute, RDA. Six isoenzymes (esterase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase, acid phosphatase, α-amylase) from blood and midgut were employed to construct dendrograms (UPGMA method) using a polyacrylamide gel electrophoresis. A cluster analysis revealed four major groups out of 141 races : 1) a Chinese group (group 2), 2) two Japanese group (group 1 and 3), and 3) the most distinct group 4. This current data showed that the Chinese and Japanese assemblage contained races originated from China and Japan respectively. Therefore, these distinctive groupings supported that the four major group associations were strongly originated geographically. Frequencies of correlated presence and absence of alleles with geography suggested that marked differences exist at esterase (Est1, Est2) and general protein (from blood) for groups 2 and 3, and a α-amylase allele present at a high/low frequency in groups 1, 2 and 3. Characteristics of group-specific enzymes were able to resolve the relationships between genotype and geographic origin as markers effectively.

동위효소 추가 분석에 의한 누에 품종분류의 재고

성수일 수원대학교 자연과학연구소 2001 자연과학논문집 Vol.4 No.-

The previous study, using six isozymes with 20 loci, revealed genetic relationships among 303 silkworm stocks preserved in the Department of Sericulture and Entomology, NIAST, RDA. As a part of additional studies, three additional isozymes with 7 loci [phosphoglucomutase(PGM), glucose phosphate isomerase(GPI), malete dehydrogenase(MDH)] from hemolymphs, midguts, and eggs were employed to reevaluate the previous dendogram (UPGMA method). Although the current study supported the recognition of the previous major groups, some minor changes were apparent among the selected 47 forms. This study showed that GPI from eggs and MDH from hemolymph may be important factors for generating a new major group in the genetic relationships occurred by additive isozyme analysis. Interpreting the results in light of evidence from the current study, this may, in the future, represent more reliable criterion for 303 silkworm stocks preserved in Korea.

누에와 천잠의 α-glucosidase 활성 비교연구

성수일 수원대학교 기능성생명소재연구소 2007 자연과학연구논문집 Vol.6 No.1

α-Glucosidase activities in the hemolymph and midgut of the mulberry silkworm, Bombyx mori, and Japanese oak silkmoth, .Antheraea yamamai were determined using various substrates such as maltose, sucrose, trehalose, and ρ-nitrophenyl α -D-glucopyranoside. The a-glucosidase activities in the hemolymph of B. mori were in general higher than those in the hemolymph of A. yamamai, however, the enzyme activities in the midgut of A. yamamai were higher than those in the midgut of B. mori for all or the substrates tested. In addition, the effect or pH and temperature on a-glucosidase activities in the midgut were similar between B. mori and A. yamamai for all or the substrates tested. The migration patterns or the a -glucosidases detected by native PAGE were different between B. mori and A. yamamai, suggesting that the molecular structure or a-glucosidases or both insects may be different in spite or the similarity or Physiological function.

동위효소 다형특성에 의한 누에 품종의 유연관계

성수일 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2

Isozyme was used to characterize general protein patterns of genetic relationships among 303silkworm stockf preserved in National 誇ericultural and Entomology Research Institute, RDA.Six isozymes (esterase, acid phosphatase, alkaline phosphatase, amylase, glucose-6-phosphatedehydrogenase and sucrase) from hemolymph, midgut, and digestive juice were employed toconstruct dendograms(UPGMA method) using a polyacrylamide Eel electrophoresis. A clusteranalysis revealed fnur major groups out of 303 strains. The current data showed that the firstand the second group, which were divided into sneral subgroups within each group, rontainedassemblages of Japanese and Chinese races. Especially, genetic differentiation in the nrst andsecond group was greatest rather than within Japanese and Chinese races repectively and wasconcordant with the hypothesis of phyletic sorting of initial variability in China many yearsago. Hypothesized recent introgression between groups was also plausible, but the evidencesuggested bidirectional gene flow between the the thinese and the Japanese lineages.Interpreting the results in light of evidence from the current study, the genetic diversity andrelationship showed in Korean silkworm race, Hansammyun reflected early and independentevolution from the Chinese ancestor, limited addition of new variability and phyletic sortingwithin Korean penin$ula more than 4,000 years.

鱗翅目 昆蟲의 成蟲體液蛋白質에 관한 生理生化學的 硏究 : Ⅰ.家蠶의 성충특이체액단백질의 검출 Ⅰ.Appearance of Adult Specific Proteins in the Hemolymph of the Silkworm, Bombyx mori.

成洙一,文在裕,李相夢,尹馨珠 한국잠사학회 1988 한국잠사곤충학회지 Vol.30 No.1

Adult specific proteins, referred to as ASP-I (adult specific protein of slow mobility) aud ASP-II (adult specific protein of fast mobility) at the pharate adult stage of the silkworm, Bombyx mori, were detected by polyacrylamide gel electrophoresis. The adult specific proteins(ASP-I and ASP-II) were defined as a kind of adult hemolymph proteins without sex specificity, and there was no variation in the respective electrophoretic mobility of ASP-I and of ASP-II among the forty-one silkworm varieties. tested.

Polyhydroxylated Alkaloids 생산균주의 분리 및 동정

성수일 수원대학교 기능성생명소재연구소 2004 자연과학연구논문집 Vol.3 No.1

Over one hundred polyhydroxylated alkaloids have been isolated from various kinds of plants and microorganisms. The alkaloids are known as a potential inhibiting ability against glycosidase, which is involved in a wide range of important biological processes such as in the intestinal digestion, post-translational processing of glycoproteins and lysosomal catabolism of glycoconjugates. 1-Deoxynojirimycin(DNJ), one of the piperidine alkaloids, has been focused an its strong α-glucosidase-inhibiting activity as well as its potent suppressive reaction to the viral maturation. In recent, DNJ was particularly applied to the treatment of diabetes due to its reducing power of diet-induced hyperglycemia by inhibiting intestinal α-glucosidase. Thus, the available resources were investigated in order to get large amounts of DNJ. The bacterial strain was isolated MORI-91 from the cereal. Strain MORI-91 mainly produced DNJ as well as other polyhydroxylated alkaloids with high potency of α-glucosidase-inhibiting activity. A bacterium producing DNJ was identified as Bacillus subtilis by means of a quinone analysis, fatty acids composition analysis in a whole cell content, sugar utilization tests, a partial 16S rRNA sequencing data, and a scanning electron micrograph of cellular morphology.

인도네시아와 베트남산 식물 추출물의 tyrosinase 억제활성 조사

성수일 수원대학교 기능성생명소재연구소 2005 자연과학연구논문집 Vol.4 No.1

In order to screen natural compounds which inhibit melanine synthesis, the extracts of 77 Indonesian plants and 42 Vietnam plants was investigated for mushroom tyrosinase inhibitory activity. The extracts of four plants, Pachyrrhizus erosus, Zingiber officinale. Curcuma domestica, and Desmodium styracifolium, showed relatively high mushroom tyrosinase inhibitory activity. Some experiments are now carrying out to isolate and identify the phytochemical compounds for the biological activity.

미생물을 이용한 1-Deoxynojirmycin의 생산 및 활용방안에 관한 연구

성수일 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

1-Deoxynojirimycin(DNJ)은 alkaloids의 한 성분으로 혈당강하, 항바이러스 등 의 효과가 알려지고 있다. 즉 DNJ는 α-glucosidase의 저해기능을 통해 소장에서의 이당류의 분해·흡수를 억제함으로써 식 후 혈당의 조절이 가능하고 또한 바이러스 복제에 필요한 단백질의 합성을 차단함으로써 바이러스 증식을 억제하는 항바이 러스 효과를 보이고 있다. 이러한 DNJ의 기능에 기초하여 현재 당뇨환자 및 바이러 스성 가축 질병에 대한 산업적 이용이 이루어지고 있다. 그러나 DNJ는 매우 고가품으로서 이 물질의 경제적생산방법의 개발이 요구되 고 있다. DNJ 생산에는 뽕잎이나 누에로부터의 추출법, 화학적 합성법 및 미생물 배양법 등이 알려져 있으나 이 가운데 미생물 배양에 의한 DNJ 생산이 경제성이나 물질의 안정성 및 분리정제의 간편성 등 여러 면에서 유용한 것으로 확인되고 있다. 본 발표에서는 DNJ 생산균주의 분리와 동정, DNJ의 정제와 유전자 확인 및 산업적 활용 방안 등 DNJ에 관한 일련의 연구결과를 소개하고자 한다. 미생물 중에서는 바실러스균, 방선균 등이 DNJ를 생산하는 것으로 알려지고 있 는데 본 연구진은 전통식품인 청국장에서 수백 종의 바실러스균을 분리하고 그 중에 서 α-glucosidase에 대한 저해능이 우수한 몇 개의 균주를 선발하였다. 이들 균주 가 운데 효소 저해능이 가장 우수한 하나의 균주를 최종 선발하고 이 균에 대한 생화학 적 및 분자생물학적 동정을 한 결과 이 균주는 Bacillus subtilis와 99%의 유전적 상동 성을 보였으며 선발된 이 균주를 B. subtilis MORI로 명명하였다. 본 균주에 의해 생 산된 α-glucosidase 저해물질의 화학적 동정을 위해 이온교환수지 크로마토그래피 법 등 일련의 분리정제를 시행하여 순도 98%이상의 DNJ를 얻고 이어서 HPLC, GC-MS, NMR 등의 기기분석을 통해 이 물질이 DNJ와 동일한 물질임을 확인하였다. 그러나 이와같은 단순한 미생물 배양법은 DNJ 생산량이 1g/L 정도로 수율이 낮 아 보다 높은 수율의 DNJ 생산을 위한 유전공학적 생산 전략을 구상하였다. 이 목 적을 위해 먼저 MORI균으로부터 DNJ 생합성 유전자를 탐색하였다. 우선 MORI 균주에서 분리한 게놈을 BamHⅠ으로 partial digestion하여 BAC libraray를 제작 하고 이 library로부터 α-glucosidase 저해능을 갖는 대장균 클론 C36-4를 선발하였 다. 다음 이 클론이 생성하는 α-glucosidase 저해물질을 HPLC와 GC-MS 기기분석한 결과 DNJ와 동일한 물질임이 확인되었다. 클론 C36-4가 보유하고 있는 삽입 유 전자의 염기서열을 분석한 결과 10 kb크기의 DNA 안에 Bacillus amyloliquefaciens FZB42 균주와 유전적 상동성이 높은 gabT1, yktc1, gutB1 등 3종의 유전자가 존재함 을 확인하였고 대장균을 이용한 이들 유전자의 발현을 조사한 결과 대장균에서 실제 DNJ가 생합성 되고 있음을 확인하였다. 현재 대장균 또는 바실러스균 등의 발현용 미 생물에 이들 유전자를 도입하여 DNJ의 대량생산을 위한 연구가 진행 중에 있다. DNJ의 산업적 활용으로는 MORI균주의 대두발효추출물(BTD-1)에서 제1형 및 제2형 당뇨동물의 혈당강하 효과를 확인하여 현재 의료기관에서 혈당조절관련 인체적용시험이 진행 중에 있으며 한편 축산농가에서는 이 발효추출물을 가축의 바이러스성 질병 예방 및 발육개선을 위한 사료 첨가제로 사용하고 있다. 이밖에 지 방세포 유래의 사이토카인 성분의 하나인 adiponectin의 발현, 분비, 증대 등에 미 치는 DNJ의 작용을 통해 심혈관계 질환 개선, 당뇨합병증 개선, 비만 개선 등 DNJ 의 활용 확대방안 모색을 위한 연구가 수행 중에 있다.

인도네시아 및 베트남 산 식물 추출물의 Lipase 활성억제 조사

성수일 수원대학교 기능성생명소재연구소 2006 자연과학연구논문집 Vol.5 No.1

In order to find new resources of lipase inhibitors, the methanol extracts from seventy eight and forty two plants raised in Indonesia and in Vietnam respectively were screened for their anti-lipase activity. Among the plants tested. Hippeastrum puniceum from Indonesia exhibited the highest lipase inhibitory activity. The extract of H. puniceum was partitioned successively with hexane, ethyl acetate and butanol, from which butanol fraction showed relatively high lipase inhibitory activity. From the data based on the physio-chemical analysis, the enzyme-inhibiting compound is considered to be a large molecule with the property of polarity.