동종 골수이식 후 만성 이식편대숙주반응 환자에서 발생한 폐렴구균에 의한 수막뇌염 1예

신완식,김병욱,유진홍,김동집,김춘추,박종원,이종욱,김동욱,강문원,김양리 대한감염학회 1993 감염 Vol.25 No.3

Graft-versus-host disease (GVHD) is a frequent complication after bone marrow transplantation. Infectious complications are common in GVHD patients due to defect in cell-mediated immunity. A rare case of S. pneumoniae meningoencephalitis occured in a patient with extensive form of chronic GVHD after allogeneic bone marrow transplantation. He was immediately treated with full dosage of ceftriaxone and ampicillin. He suffered from various complications such as sepsis, acute renal failure, atelectasis, and seizure. Despite of aggressive treatment, he died probably due to renal shutdown and massive subacute cerebral infarction of left cerebral hemisphere. This report showed two unusual and rare features. First, the infection site was CNS rather than respiratory system. Second, the causative organism was S. pneumoniae, which is rare cause of CNS infection in immunocompromised patients.

플라즈마 표지소자의 제작

이상윤,라병욱,박동수,황인헌,이덕동,신영남,박성배,이동욱,박용석,박형근,손상호,권태근,채경락,정경득 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

An Ac-type Plasma Display Panel (PDP) operating with Ne-Ar(0.1%) Penning mixture gas is fabicated. The characterics of the panel with electrodes covered with thin and thick dielectric layers are studied. The brightness of the Neon-orange light emitted by the panel measured as function of applied voltage and frequency. As an application, a graphic display system equipped with PDP showing still and moving pictures is made.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

군 훈련병의 스트레스, 성인 주의력결핍 과잉행동장애 증상과 자살사고의 연관성에 관한 연구

이동윤,이철순,박철수,손진욱,김봉조,차보석,이소진 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study is conducted to investigate the effect of Attention Deficit and Hyperactivity Disorder(ADHD) symptoms on stress and suicide idea among 224 conscripts. Methods:Total number of subject is 224 conscripts. We evaluate symptoms of adult ADHD with Korean-Wender Utah rating scale(K-WURS) and Korean adult attention-deficit/hyperactivity disorder scale(K-AADHDS), stress with Brief Encounter Psychosocial Instrument-K(BEPSI-K), suicide idea with Scale for suicide ideation(SSI) after basic military training. Multivariate logistic analysis with backward stepwise selection is performed to evaluate risk factors of stress. Multiple linear regression analysis with stepwise selection is performed to evaluate association of suicide idea with adult ADHD symptoms. Results:Stress is significantly associated with K-WURS score, K-AADHDS score and SSI score(p<0.01, p<0.05, p<0.01). K-WURS score are significant risk factor of stress after basic military training(p<0.05, O.R : 1.034, 95% CI : 1.005-1.065). And K-WURS score influence suicide idea after basic military training(p<0.05, β : 0.031). Conclusion:The result suggests that K-WURS score might be a risk factor of stress and could affect suicide idea.

베체트병에서 보이는 혈관질환

박우일,김동익,조진현,이병붕,신성욱,도영수 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.2

셀레콕시브 및 그 합성유도체들의 항암활성 스크리닝

박정란,강진형,구효정,노지영,류형철,박상욱,고동현,조일환,이주영,황다니엘,김인경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at 1μM after 24 hr exposure. Forty μM and 50μM of celecoxib induce G_1 arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

Expression of Rb in Human Middle Ear Cholesteatoma

박수연,이동욱 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.2

목적 : 중이귀진주종의 병리조직학적 특징은 진주종 상피의 과다증식과 비정상적인 과각질화라고 요약되는데 이러한 현상은 진주종 상피를 형성하는 각질세포의 증식, 분화 및 세포 사멸로 이루어지는 세포주기의 항상성 변화로 기인한다. 본 연구는 세포자멸사(apoptsis) 및 세포 주기의 조절에 중요한 영향을 미치는 것으로 알려져 있는 Rb의 가운데귀진주종 상피 내에서의 발현 양상을 알아보고자 시행되었다. 방법 : 만성 진주종성 중이염으로 수술을 시행 받았던 환자 10예의 진주종 상피와 정상 뒤귓바퀴 및 목의 피부조직 5예를 대상으로 Rb에 대한 Western blot analysis를 시행하였고, 중이 진주종 조직 6예와 정상 뒤귓바퀴 조직 4예에서 Rb의 발현 양상을 면역조직화학적 검사를 통하여 관찰하였다. 결과 : Western blot analysis 상 가운데귀진주종 상피에서는 10예 중 9예에서 Rb가 발현되었고 대조군으로 사용된 정상 피부조직에서는 5예 중 1예에서만 양성 반응을 보였다. 면역조직화학적 검사에서 가운데귀진주종 상피에서는 Rb 양성인 상피 세포가 바닥층과 바닥위층에 넓게 분포하고 있는 것이 관찰되었고 Rb의 발현 정도는 정상 상피에 비하여 증가되어 있었다. 반면 정상 상피에서는 Rb 양성인 상피세포는 바닥층에서만 부분적으로 관찰되었고 그 발현의 정도는 진주종 상피에 비하여 감소되어 있었다. 이는 통계적으로 유의하였다. 이상의 결과로 Rb가 가운데귀진주종 상피에서 비정상적으로 과발현 되고 있음을 확인할 수 있었다. 결론 : 진주종의 중요한 특징인 상피 세포의 과증식과 비정상적인 과각질화는 각질세포의 세포주기 항상성 및 세포사멸 현상의 이상에 의한 것으로 생각되었으며 세포세포자멸사 및 세포 주기의 조절에 중요한 역할을 하는 것으로 알려진 Rb가 중이 진주종의 병인에 중요한 역할을 할 것으로 판단되었다.

수중운동(Aquatic Exffect)의 원리와 효과

박종욱,박동수,박희석 대불대학교 2000 論文集 Vol.6 No.1

Aquatic exercise can be defined as the exercise to performing in underwater all exercise type on the ground without burden and danger of exercise injury. The aquatic exercise is effective exercise program in decreased of weight, low-back pain, obesity index, and also increased of cardiovascular endurance, muscle endurance, VO_(2) max, range of joint flexion, flexibility without pain in joint and low-back by the minimum of weigh load due to specificity of water. Especially, the aquatic exercise is exercise type applicable to safely in people, which were patients with low-back pain, Arthritis patients, Arthrite for training and rehabilitation, Obese people because of the limited activities and exercise, Elderly decreased of range of joint motions, through the recomposition of exercise intensity and program. The effect of exercise in underwater is very elevated because of various kinds specificity of water in underwater. Therefore, to the greatest effect of exercise should be progressively training a spot separately according to the need of oneself, also should be pay attention to details as following : ① Appropriate intensity of exercise is important to the suitable to one's fitness level. ② Repetitive movements request to increased of exercise intensity, frequency, duration. ③ Contact angle with water when the arm and leg swing is important to maintenance right angle. ④ Appropriate supplement of water is positively necessitate.

실리카지지 루테늄에서 일산화탄소와 산소 공동흡착에 관한 FTIRS 연구

박상윤,윤구식,윤동욱 울산대학교 2002 자연과학논문집 Vol.12 No.1

살리카 지지 루테늄에서 일산화탄소의 압력, 루테늄 함량, 흡착온도를 변화시키면서 적외선 스펙트럼을 관찰했다. 또한 실리카 지지 루테늄에서 일산화탄소와 산소의 혼합기체의 분압비를 변화시키면서 적외선 스펙트럼을 관찰했다. 실리카 지지 루테늄에 일산화탄소를 흡착시켰을 때 2004.2-2038.9 ㎝^-1, 2077.5∼2089.0 ㎝^-1, 2141.1∼2145.0 ㎝^-1 세 흡수띠가 관찰되었다. 관찰된 스펙트럼에서 흡수띠의 위치는 문헌에 보고된 바와 거의 일치한다 2077.5∼2089.0 ㎝^-1와 2141.1∼2145.0 ㎝^-1 두 흡수띠의 흡수세기가 산소의 분압을 증가시켰을 때 증가했다. 본 연구의 결과는 실리카 지지 루테늄에 일산화탄소와 산소 혼합기체를 흡착시켰을 때 나타나는 ∼2130 ㎝^-1, 흡수띠와 ∼2080 ㎝^-1 흡수띠는 각각 산화된 루테늄 원자와 그 영향을 받는 루테늄 원자에 흡착된 일산화탄소에서 CO의 신축진동에 의한 것이라는 Brown 등의 주장을 뒷받침한다. The infrared spectra for CO adsorbed on silica supported ruthenium have been investigated at various partial pressures and temperatures within the frequency range of 1500∼2300 ㎝^-1. Also the infrared spectra for CO/O_2 gas mixtures adsorbed on silica supported ruthenium have been investigated at various partial pressure ratios within the frequency range of 1500∼2300 ㎝^-1. Three bands (2004.2-2038.9 ㎝^-1, 2077.5∼2089.0 ㎝^-1, 2141.1∼2145.0 ㎝^-1) were observed when CO and O_2 were coadsorbed on silica supported ruthenium. The frequencies of the bands observed correspond with the previous reports approximately. The relative intensities of 2077.5∼2089.0 ㎝^-1 band and 2141.1∼2145.0 ㎝^-1 band increased with raising O_2 partial pressure. These results support Brown and Gonzalez's argument that ∼2080 ㎝^-1 band and ∼2130 ㎝^-1 band are arised from the CO stretching vibration for CO molecules adsorbed on the ruthenium atoms perturbed by the oxygen atoms bonded to the ruthenium atoms and on the oxidized ruthenium atoms respectively.