Alcohol extract of Geum Chung Cho alleviates alcohol-induced short-term memory impairment in gerbils

Han-Sam Cho,Tae-Woon Kim,Sang-Seo Park,Mal-Soon Shin,Jae-Min Lee,Eun-Sang Ji,Chang-Ju Kim 한국운동재활학회 2015 한국운동재활학회 학술대회 Vol.2015 No.10

Chronic ethanol consumption leads to memory impairment and also induces brain damage. The hippocampus has been particularly regarded as a possible target organ of alcoholic intoxication, since this structure plays a prominent function in memory processes. Cordyceps has been used as a traditional medicine in Asian countries for a long time. Different types of Cordyceps extract were reported to have various pharmacological activities including anti-tumor, anti-inflammatory, anti-viral, and immune modulatory effects. Guem Chung Cho is an artificial cultivated Cordyceps, and was supplied from GCordy (Seoul, Korea). Ethanol extract of Cordycep (50 g) was made by rotary evaporate, and resulting powder 8.29 g was obtained (yield 17%). In the present study, we investigated the effects of ethanol extract of Geum Chung Cho on short-term memory, cell proliferation and apoptosis in the hippocampus of ethanol-treated gerbils. The gerbils were divided into 5 groups (n = 10 in each group): Control group, alcohol-treated group, alcohol-treated and 0.001 mg/kg Geum Chung Cho-applied group, alcohol-treated and 0.01 mg/kg Geum Chung Cho-applied group, and alcohol-treated and 0.1 mg/kg Geum Chung Cho-applied group. Gerbils in the alcohol-treated groups were orally administered ethanol (1 g/kg) once a day for 2 weeks. Gerbils in the Guem Chung Cho-applied groups were orally administered Guem Chung Cho extract at each dosage 1 hour before ethanol consumption. Our results revealed that consumption of alcohol for 2 weeks deteriorated short-term memory, induced apoptosis, and inhibited cell proliferation, and reduced expressions of brain-derived neurotrophic (BDNF) and tyrosine kinase B (TrkB). However, Guem Chung Cho treatment alleviated alcohol-induced short-term memory impairment through enhancing cell proliferation and BDNF expression with inhibiting apoptosis in the hippocampus. Guem Chung Cho showed maximum effects at 0.1 mg/kg. Based on these results, alcohol extract of Guem Chung Cho may provide therapeutic value for the treatment of chronic alcohol-inducted brain injury. This study was supported by GCordy company.

Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Cho, Kyu-Hyuk,Cho, Jae-Woo,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 mg/kg ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in $G_1,\;G_2\;and\;G_3$ mice. One hundred thirty five $G_1$ phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen $G_3$ recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

만성 화농성 중이염의 세균학적 고찰

조남순,이영훈,강기훈,최제환,송태현,이병돈,장혁순,강주원,김연준 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and Objectives : Chronic suppurative otitis media is one of the frequent diseases in otolaryngologic field. It is important to choose of antibiotics in the management of infectious disease. But the organisms in infections have been changed and resistance to antibiotics has been grown as the development of antibiotics has been achieved. And so it has been necessary to recognize the changes of organisms and resistance in antibiotics. Our study was performed to identify the pathogens isolated from discharges in chronic otitis media and evaluate the antibiotic agents, to recognize the change of to resistance to·antibiotic agents and to use the appropriate anitiotics. Materials and Methods : Retrospectively, authors reviewed the backeriologic study of 114 cases of chronic suppurative otitis media who visited the Department of Otolaryngology, Soonchunhyang University in Seoul from Nov. 1996 to Oct. 1999 and analyzed 101 strains and its sensitivity test to various antibiotics. Result : In 93 cases in which pathogenic organism was isolated, single infection was 85 cases(91.4%) and mixed infection was 8 cases(8.6%). The most frequent pathogenic organism was Staphylococcus aureus(59.4%), Pseudomonas aeruginosa(13.9%), Providencia(4.8%), Alcaligenes(3.0%) and Streptococcus pneumoniae(2.9%) were the next. Methicilline-Resistance Staphylococcus aureus was 34 cases(57.6%) of Staphylococcus aureus. Staphylococcus aureus was sensitive to Vancomycin(96.7%), Trimethoprim-Sulfamethoxazole (57.6%), Cefuroxime(42.4%) and Ciprofloxacin(40.7%) but resistant to Penicillin(94.9%). Pseudomonas aeruginosa was sentitive Amikacin(100%), Ciprofloxacin(100%) and Gentamicin(67%). Conclusion : As MRSA recently was wide spread in community accquired infection as well as nosocomial infection, we must consider strict control of MRSA.

Poster Session:PS 1203 ; Cardiology : Predictive Factors of Reversibility in Patients with Severe Tricuspid Regurgitation

( Jae Yeong Cho ),( Kye Hun Kim ),( Kyung Jin Lee ),( Yi Rang Yim ),( Sung Soo Kim ),( Hae Chang Jeong ),( Ki Hong Lee ),( Keun Ho Park ),( Doo Sun Sim ),( Hyun Ju Yoon ),( Nam Sik Yun ),( Young Joon 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background:Since severe tricuspid regurgitation (TR) is an uncommon fi nding which develops in various conditions, the present study aimed to investigate etiology, clinical characteristics, and predictors of reversibility in patients with severe TR. Methods:A total of 232 patients (67. 4±14. 1 years, 80 males) who were diagnosed with severe TR by echocardiography were enrolled. Severe TR was defi ned as vena contracta width greater than 0. 7 cm and systolic fi ow reversal in hepatic veins according to the current guideline of American Society of Echocardiography (ASE). Improvement of TR to moderate or less degree on follow up echocardiography was considered as reversible TR in the present study. Primary end points were adverse events at long-term follow-up. Adverse events were defi ned as all-cause death and operation due to severe TR. Results: Reversible TR was observed in 35 out of 153 patients (23. 0%). Sixty-one patients showed clinical improvement, but the degree of TR was not changed. Forty-nine patients (32%) who did not show clinical improvement despite of medical therapy eventually underwent surgical correction of TR, and 7 patients died. In addition, 17 patients died without operation, so 24 deaths (16%) were observed in total. finally, total adverse events developed in 66 patients (41%) during 2 years of follow-up period. Logistic regression analysis revealed prosthetic mitral valve was the only independent predictors of future adverse events (OR 2. 47, 95%CI 1. 05-5. 77, p=0. 038). Independent predictors of improved TR turned out to be the use of RAAS blockade (HR 3. 02, 95%CI 1. 12-8. 17, p=0. 030) and the use of spironolactone (HR 3. 39, 95%CI 1. 05-10. 90, p=0. 041). Low ejection fraction (LVEF <50%) also had a trend toward the reversibility (HR 2. 82, 95%CI 0. 94-8. 40). Conclusions:Considering results above, reversible severe TR mainly associated with left heart failure and medical treatment should be given before considering surgery for severe TR.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

이상기후 발생이 가을배추 생산자 및 소비자에게 미치는 영향

조재환 ( Jae Hwan Cho ),서정민 ( Jeong Min Suh ),강점순 ( Jum Soon Kang ),홍창오 ( Chang Oh Hong ),신현무 ( Hyun Moo Shin ),이상규 ( Sang Gyu Lee ),임우택 ( Woo Taik Lim ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.12

The purpose of this article is analyzing the economic impacts of abnormal climate on fall chinese cabbage farmers and consumers in Korea, with employing the equilibrium displacement model. Our results show that there were little difference in gross farm income, even though there were significant yield reductions due to abnormal climate changes. However periodic occurrences of abnormal climates caused serious damage to consumption levels which had declined by 10.6~17.1 percent with higher prices by 15.3~24.6 percent than normal climate years since 1990.

한국·미국·스페인 청소년의 태권도 수련이 인성교육에 미치는 영향

유창재,양정옥,이중숙,이상돈,김영수,조만태 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

The purpose of this study was to analyzed what effect Taekwondo training has upon Juvenile trainees in Korea, America, and Spain. to see if there is a significant difference in their Human nature Education. The subject of this study was set up as 547 Juvenile trainees who are including 183 Koreans, 185 Americans and 179 Spanish people belonging to gymnasia in their own countries, and sampling by purpose sampling. The means in this study is used to amend and complemented after confirming suitability of the contents in the questionnaire that is made on the basis of questionnaire Lee Chul Ho(1996). it is used as study means after modified, whether the substance of question is right in fitness and applicability or not, Reliability of the question, the range of Cronbach's a is showed from .7924 to .8450. The method of statistically analysis adapted one-way ANOVA, two-way ANOVA by using SPSS WIN 10.0 Program. The result of this study are as followed : First, it can be seen that American Juvenile and Spanish Juvenile show more significant change than Korean Juvenile in their Human nature Education by the Taekwondo training according to their nationality. Second, it can be seen that Juvenile who have had the Taekwondo training for more than from three to five year show more significant change in their emotion, sociability and attitude towards life than those who have had the same training for more than other period in their Human nature Education according to their Taekwondo training period. Third, as a result of analytic investigation of what effect the same training has upon the change factors in their Human nature Education in accordance with their training period and nationality, it can be seen that the training itself and nationality has the greater influence on them than the training period. Therefore, judging from the above findings, it can be proved that the Taekwondo training has much more significant and positive effect on Juvenile in their change of emotion, sociability and attitude towards life.

안면신경마비에 대한 안륜근 미소안면침 증례보고

조아름,김지현,주경옥,원재선,김창환,Cho, Ah-Reum,Kim, Ji-Hyun,Chou, Ching-Yu,Won, Jae-Sun,Kim, Chang-Hwan 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.2

Objective : This study was designed to evaluate the effect of Miso Facial Acupuncture on Orbicularis Oculi muscle. Methods : Two men and four women with Facial nerve palsy were recruited. Miso Facial Acupuncture on Orbicularis Oculi muscle was performed two times a week for three weeks. The effects of the treatment were evaluated by H-B scale, Y-system and clinical symptom. Results : Before treatment with Miso Facial Acupuncture, three patients showed H-B scale improvement and the other three patients had no change. and four patients showed Y-system improvement and the other two patients had no change. In six cases, the patients had improvement in clinical symptom related with Orbicularis Oculi muscle function. Conclusion : Miso Facial Acupuncture on Orbicularis Oculi muscle showed significant improvement in facial nerve palsy.

β-Carotene과 알코올을 섭취한 쥐의 혈액 성분변화의 상관관계

고재웅,조만희,이상한,우기민,염윤기,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The study was carried out to investigate the effects of dietary carotenids and β-carotene on the hematological changes caused by alcohol administration into the rats. The results were analyzed using several statistical methods; SPSS computer program for measuring the mean values and standard deviations, one way ANOVA for the test of significance, Duncan method for post HOC multiple comparison, and Pearson method for measuring the correlation coefficient. The following are the conclusion from those statistical analysis. 1. ALB and TB were shown to have the most significant correlation among the blood compositions f개m the basic dietary groups. 2. BUN and CHO were shown to have the most positive correlation among those from the carrot-fed groups. 3. CA and AG were shown positively correlated, and CHO and IP negatively correlated, respectively among those from the cheese-fed groups. 4. AP was shown positively correlated among those from the alcohol-administrated groups. 5. CHO, CA and BUN were shown positively correlated, and CA was negatively correlated among those from the β-Carotene -fed groups. 6. TB and TP were not affected by the correlations between the blood composition from all the experimental groups.

강원도지역에서 발생한 발진열과 쯔쯔가무시병

민창홍,장우현,강재승,조수익,최문기,조민기,윤창순,김윤원 대한감염학회 1988 감염 Vol.20 No.2

In Korea, there has been an increasing concern on rickettsiosis as one of most prevailing acute febrile illness, since murine typhus and scrub typhus had not been reported for 2 decades until 1986 and thereafter a few studies were reported on these diseases. A microimmunofluorescence test was used to study antibodies to R. typhi and R. tsutsugamushi in urban and rural inhabitants students, and patiants suspected of leptospirosis or rickettsiosis from 1986 to 1987 in Kangwon-do Korea. 43% of inhabitatnts and 17% of students reacted to R. typhi. Only one inhabitant among inhabitants and students reacted to R. tsutsugamushi. Among 169 patients, 43(25%) reacted to R. typhi, 38(22%) to R. tsutsugamushi and 25(15%) to Leptospira interrogans. Sexual rate(male; female) in murine typhus was 1.7:1, that in scrub typhus was 1:2.2 and that in leptospirosis was 1.5;1. The age ditribution of patients with murine typhus was generally even over 20 years old. But 71% of patients with scrub typhus were over 40 years old. Scrub typhus was most prevailing in female over 40 years old. Murine typhus was relatively more prevailing in Choonchun city than rural area as compared with scrub typhus and leptospirosis. Most of scrub typhus occurred in rural area. Most of each disease occurred between September and November (murine typhus; 93%, scrub typhus; 95%, leptospirosis; 84%) with a peak in October. Scrub typhus considerably occurred not only October(58%) but also November(28%). All patients with scrub typhus were immediately diagnosed by antibody when they visited hospital. 53% of patients with murine typhus were diagnosed when they visited hospital within 7 days after onset of illness, and others were done by second sera obtained 8 days after. The patients in whom IgM was seronegative but antibodies of other class were seropsitive by 20 days after onset of illness, were 14% in murine typhus and 26% in scrub typhus. At least those patients were considered to be infected second. Murine typhus and scrub typhus were suggested to be endemic diseases in Kangwon-do as antibodies of inhabitants and characteristics of patients' sera were considered. By serologic finding of patients with scrub typhus in Kangwon-do, Gilliam was 59'% of etiologic strain of scrub typhus, Karp was 41q% and Kato was none. Most scrub typhus occurred in person to be related to agriculture, but murine typhus was not related to job and occurred more in small city than rural area. Rash was scarcely detected in murine typhus and scrub typhus, and eschar was in 50% of scrub typhus only. Serum transaminase elevated in many patients with scrub typhus and murine typhus. Leukocytosis and abnormal finding in chest X-ray were showed in some patients with scrub typhus but were scarcely in murine typhus.