취업모 가정의 유아 컴퓨터 활동 환경 연구

이재연,신소영 숙명여자대학교 아동연구소 2000 兒童硏究 Vol.14 No.1

The purpose of this study was to examine the children's computer activity concerning their working mother's recognition, home environment and current operation at their home. 109 working mothers who sampled in 5 day care centers were selected as the subjects of this study. This study was performed by the questionnaire made by this researcher, and the data were analyzed with Frequency, Percentage. The results of this study are as follows, Working mother's recognition on the need of young children's computer activity was positive(91.8%0. Working mother thought that young children's computer activity was needed for young children's various experiences(24.7%) and the preparation for the future life(22.6%). The most important objective of young children's computer activity was to help children get familiar with the computer(30.3%). And working mothers recognized that the effect of young children's computer activity was to promote their children's originality, imagination and insight. The environment of young children's computer activity in the working mother's home was sufficient(75.3%). Most have their own computer hardware and peripheral equipment. And the computer's installation environment was set up well. Young children did computer activities with parents(33.3%) or brothers(47.4%) at their livingroom. It took one hour for young children to do the computer activity at home(75.7%). The degree of possession of the software in working mother's home was 1-5(50.0%). Working mother put a software at young children's computer activity from the beginning(65.4%). And they confirmed the contents of the software. A standard of buying the software was mainly inducement that people had ever that software(24.1%). Working mother recognized on the possessing software that were appropriate for their children's age and educational. An obstacle about children's computer activities in their home was shown to be insufficient of possessing appropriate software for the child. For the activation of the children's computer activity, it was required to possess various and appropriate softwares. For children's computer activity was connected with day care center's, working mother wanted to take part in parent's seminar(36.6%) or participation class for parents(23.4%).

은행나무의 잎끝마름병에 미치는 SO2 의 영향

이두형,배공영,박소홍,강연지 한국환경농학회 1999 한국환경농학회지 Vol.18 No.2

대기오염과 병충해발생과의 관계를 규명하고자 은행나무에 SO₂가스를 처리한 후, 잎끝마름병균을 접종하여 식물의 생리·생화학적인 변화를 조사하였다. 0.1㎕/ℓ의 SO₂가스를 하루 8시간씩 7일간 은행나무에 처리한 결과 Chlorosis등의 가시피해는 나타나지 않았으나, 광합성이 대조구에 비해 40%가량 감소되고, CO₂이용효율과 수분이용효율이 감소되는 현상을 보였다. 가스처리 후 병원균을 상처접종시키고 42일 뒤 SI와 SFI의 발병도를 조사한 결과 SI가 SFI에 비해 3배 가량 빠르게 병이 진전되어 있었다. 병원균의 먹이가 되는 당의 함량은 대조구, SFI, SI간에 유의성을 보일 만큼의 차이는 보이지 않았으나, 광합성은 SI가 SFI에 비해 11%가량 저하되었다. 광합성이 저하되었음에도 불구하고 당의 함량이 유지되었으므로 탄소 고정관계를 알아본 결과 CO₂ 이용효율이 저하되지 않았음을 알 수 있었다. 이와 함께 수분이용효율이 SI에서 SFI에 비해 13.2% 증가되었다. 이상의 결과를 종합해 보면, 이온유출량 등으로 보아 SO₂가스처리로 인해 식물이 연약해져 병원균에 대한 저항력이 낮아졌고, 수분이용효율의 증가로 인해 탄소 고정이 활발하게 이루어짐으로써 당이 원활히 합성되는 한편 내부적인 변화로 인해 합성된 당의 전류가 억제되어 잎에 당이 축적되었기 때문에 SI의 발병도가 훨씬 높았던 것으로 생각된다. To examine the relationship between air pollution and occurrence of disease in plants, we investigated the alteration of physiology and biochemistry of Ginkgo biloba by inoculating with Coniothyrium sp. and fumigating with 0.1㎕/ℓSO_(-2) Visual damage did not appear but photosynthesis, CO₂use efficiency(CUE) and water use efficiency(WUE) were reduced when G. biloba was exposed to SO₂for 7days (8 hours a day). When inoculated with Coniothyrium sp., the seventies of damage in G. biloba showed a threefold difference between SO₂-treatment (SI) and SO₂-free treatment (SFI) at day 42 from initial inoculation. Little difference was observed in sugar contents that may be used pathogens feed, among control, SFI and SI. In spite of the reduction in photosynthetic rate, sugar contents and CUE were maintained. WUE was enhanced 13% more at SI than SFI. The CO₂fixation boosted because of enhanced WUE, and thus sugar synthesis was not affected. In addition, sugar transport seems to be retarded for some internal alteration. Consequently, the severity of SI was more serious than that of SFI because Coniothyrium sp. easily invades into the physical texture of G. biloba weakened by SO₂fumigation and because sugar was accumulated in leaves of G. biloba.

상처받은 애착과 적대의 이동 : 김소진을 중심으로

이소영 ( Lee So-young ) 민족문학사학회·민족문학사연구소 2021 민족문학사연구 Vol.75 No.-

이 글은 국가보안법이 분단문학에서 좌익2세 작가들이 아버지를 형상화하는 수준을 결정짓는 기율로서 기능해왔다는 전제 하에 87년 체제의 성립과 국가보안법의 개정이 문학(화)장에 야기한 파문을 살펴보고자 했다. 이는 분단문학에서 아버지와 아들 중심의 관계가 더 이상 의미있는 소재가 아니었음에도 불구하고 김소진이 「쥐잡기」를 통해 분단문학의 계보를 이어나갔다는 점에 주목하게끔 한다. 김소진에 이르러 분단문학은 비로소 ‘아버지는 누구인가?’라는 물음이 아니라 끊임없이 아버지를 재현하는 아들, 즉 ‘나는 누구인가?’라는 질문으로 전환되는 것이다. 이때 김소진이 86세대라는 사실은 그의 문학이 수행했던 기억/문학의 정치성이 정체성 정치와 연동되어 있음을 짐작하게끔 한다. 87년 체제를 단순히 경제적 · 법률적 · 이데올로기적 · 공식적 제도가 아니라 사회문화적 · 비공식적 · 사적인 제도, 관계, 정체성, 의미 관계 일체를 포함하는 시스템(system)으로 간주할 때, 87년 체제의 한 요소이자 분단문학의 기율인 국가보안법이 86세대의 정체성과 밀접한 관련을 맺으면서 특정한 기억/문학의 정치성을 발휘했던 양상은 그 자체로 87년 체제의 단면을 보여주는 역동적인 드라마라고 할 수 있다. 그리고 이러한 드라마는 ‘정동(affect)’의 차원에서 제대로 포착 가능하다. 김소진 소설에서 ‘나’는 주로 상처받은 운동권으로 그려지는데, 그는 사회주의 이데올로기라는 ‘적대(antagonism)’의 상실을 아버지에 대한 애착으로 보상받고자 한다. 그러나 아버지에 대한 애착은 아버지를 부정했던 상처를 환기하는 것이었다. 이러한 ‘상처받은 애착(wounded attachment)’은 그 대상이 명확하다는 점에서 아버지의 누구도 탓할 수 없는 ‘원한(ressentiment)’과 구별된다. ‘나’는 아버지의 원한에 의해 구조화된 정체성 정치의 무력함과 거리를 두고자 하지만, 종종 아버지에게 감정 이입하면서 아버지처럼 나보다 연약한 자에게 상처를 입히는 가해자가 되어버린다. 한편, 아버지는 동시에 법의 영역에서 초연한 존재로 그려지는데, 이는 더 이상 ‘적’이 아닌 아버지가 다시 분단문학의 서사에 기입되기 위해서 취해야 했던 전략을 드러낸다. 그것은 바로 문학적인 것에 대한 단념이었다. 결국 김소진의 상처받은 애착은 적대의 자리에 대한 것이었다. 그러나 흥미롭게도 그의 소설에서 정작 적대의 대상으로 그려지는 것은 여성의 섹슈얼리티이다. 이는 김소진의 의도와 무관하게 1990년대 문학(화)장에서 적대의 대상이 이동하고 있던 양상을 보여준다. 따라서 김소진의 텍스트는, 적대의 위치에 머무르고 싶었으나 이미 대결자로 편입된 아버지와 대결자가 되고 싶었으나 여전히 적대로서 여겨졌던 여성의 섹슈얼리티 사이의 쟁투를 축약해서 보여주는 장소이다. 사라진 적대에 대한 상처받은 애착이 적대와 경합의 엇갈린 공존을 만들어낸 것이다. This treatise attempts to examine the ripples created by the establishment of the system in 1987 and the revision of the Nation Security Act and their impact on the literature landscape, on the premise that the Nation Security Act has functioned as the order that determines the degree in which second generation left-wing writers embody their fathers in division literature. This draws attention to the fact that, even while the relationship between father and son was no longer a meaningful topic in division literature, Kim So-jin persisted with the genealogy of division literature through his “Catch a Rat.” It is with Kim So-jin’s arrival that divisional literature ultimately turns into the question of “who I am” as the son that continuously reproduces the father, not the question of “who the father is.” That being noted, the fact that Kim So-jin is a member of the 86 generation allows us to conjecture that the politics of memory and literature having been performed by his literary landscape is indeed linked to identity politics. When the system in 1987 is deemed to be the system encompassing the entirety of sociocultural, informal, and private institutions, relationships, identity, and semantic relationships, not merely an economic, legal, ideological, or formal institution; it is then that the aspect of the Nation Security Act―the constituent of the system in 1987 and the discipline of divisional literature―, which demonstrated the politics of specific memories and literature while being closely connected to the identity of the 86 generation, is rendered a dynamic drama showcasing a cross section of the system. Furthermore, the drama can be properly captured from the dimension of “affect.” In Kim So-jin’s novel, the protagonist “I” is mainly portrayed as a wounded activist, and he seeks to be compensated for the loss of “antagonism,” a socialist ideology, with his attachment to his father. However, his attachment would evoke the wounds that denied his father. His “wounded attachment,” being specifically directed toward his targets, is to be distinguished from “ressentiment” of his father who could not blame anyone. The protagonist “I” intends to distance himself from the powerlessness of identity politics structured by his father’s resentment, but he would often find himself sympathizing with his father to become the same perpetrator who wounds the weak. On another note, the father is simultaneously portrayed as an unprecedented being in the realm of law, and this is to reveal his strategy that he, who is no longer deemed an “enemy,” had to take in order to be re-written in the narrative of divisional literature―his renunciation of the literature. Ultimately, Kim So-jin’s wounded attachment is towards the position of antagonism. Interestingly, however, what is indeed portrayed as the object of antagonism in Kim So-jin’s novel is women’s sexuality, perhaps indicating the object of antagonism in the literary landscape in the 1990s trending toward elsewhere, regardless of his intention. Therefore, Kim So-jin’s text summarizes to illustrate the struggle between the father who had been incorporated as a confronter even while not seeking to give up the position of antagonism, and the sexuality of a woman who sought to become a confronter but still was regarded as antagonism. The wounded attachment to antagonism in absence engendered the mixed coexistence of antagonism and agonistic.

Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

다발성 말초신경병증의 조기진단 : 적외선 체열촬영검사와 전류지각역치검사의 조합진단 Combination Diagnosis Using by Digital Infrared Thermogram and Current Perception Threshold

이삼규,김재형,최인성,이소영,한재영,조성렬,김재휴,노성만,정민영,정동진,김현성 全南大醫大 2001 전남의대학술지 Vol.37 No.3

인공신경망 모형을 이용한 울산공단지역 일 최고 SO2 농도 예측

이소영 ( So Young Lee ),김유근 ( Yoo Keun Kim ),오인보 ( In Bo Oh ),김정규 ( Jung Kyu Kim ) 한국환경과학회 2009 한국환경과학회지 Vol.18 No.2

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

고등학생의 인터넷 사용정도와 학교적응, 친구관계에 대한 연구

이현주,김미연,이희재,권소영,김민정,김민주,방은혜,이경희,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

Today, almost all families are quipped with computers. At the same time, we can find lots of side-effects because of computers. If young students, who tend to be unable to control their impulses once they are immersed in internet, the problem will be more serious. It could be happened that the young students can't distinguish the real from the image. Therefore, it is important to grasp the actual condition of juvenile Internet addicts and their practical life, and to come up with basic materials necessary for working out countermeasures. This study surveyed 339 high school students from three high schools in the area of Seoul. Data collection were collected from 22nd of December, 2003 to 27th of December 2003. The collected data were analyzed by SPSS program using T-test, ANAVA and Pearson correlation coefficient. The results were as follows: First, of the 333 respondents 9.61% of them were high-risk internet using group, 15.02% of them were potential-risk internet using group. 25% of then were identified as addicted group. Second, the more addicted the subjects are, the lower their level of school adjustment becomes. For the addicted group, it is difficult to adjust their school and to have a good relationship with friend. Third, the more addicted the subjects are, the lower their level of friendship becomes. The subordinate areas of friendship have same results as the level of Internet addiction and the extent of friendship. If students are addicted to Internet, it is difficult to find friends who can give them encouragement in practical life. Firth, there was a negative relationship between Internet addiction and school adjustment, and, there was a negative relationship between Internet addiction and friendship. Through this study we have seized the relationship between Internet addiction and school adjustment and friendship. As a result, the more addicted, the more difficult to adjust to school. And for the students who were addicted, it is difficult to make a friend.