Vibrio tapetis의 검출을 위한 PCR specific primer의 제작

김영진,이선이,조효진,유선녕,김철민,최용락,박병근,안순철,Kim, Yeong-Jin,Lee, Sun-Yi,Cho, Hyo-Jin,You, Sun-Nyung,Kim, Cheol-Min,Choi, Yong-Lark,Park, Byoung-Keun,Ahn, Soon-Cheol 한국생명과학회 2007 생명과학회지 Vol.17 No.1

Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of france, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.

자발성 흉추부 척수 탈출증 - 증례보고 -

김영진,김영수,김주헌,이형중,고용,오성훈,김광명,오석전,Kim, Young Jin,Kim, Young Soo,Kim, Ju Hun,Yi, Hyeong Joong,Ko, Yong,Oh, Sung Hun,Kim, Kwang Myung,Oh, Suck Jun 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.10

Spontaneous herniation of the spinal cord is a very rare. It's clinical symptom presents with progressive myelopathy. A 42-year old male patient who presented the progressive left leg weakness and Brown-Seqaurd syndrome is presented. MRI showed a typical finding of dural defect and herniation of the cord on the level of T3-4. Repair of dural defect using an artificial dura and reposition of cord herniation were undertaken after three level laminectomies with SSEP monitoring. Postoperatively, symptoms were improved rapidly. In our knowledgement, this is first case being reported in Korea. This entity, although rare, should be considered in the differential diagnosis of myelopathy in the absence of a mass lesion.

2014 / 2015년 동계 사료작물의 월동 후 생육조사를 통한 작황 분석

김영진,김원호,이상훈,박형수,김기용,지희정,최기춘,이상학,정종성,김지혜,황태영,이기원,김현섭,최기준,Kim, Young-Jin,Kim, Won-Ho,Lee, Sang-Hoon,Park, Hyung-Soo,Kim, Ki-Yong,Ji, Hee-Chung,Choi, Ki-Choon,Lee, Sang-Hak,Jung, Jeong-Sung,Kim, Ji-Hea,H 한국초지조사료학회 2015 한국초지조사료학회지 Vol.35 No.4

본 연구는 2014 / 2015년 동계 사료작물의 월동 후 생육조사를 통하여 전국적인 작황을 파악함으로서 사료작물 생육에 문제점이 있을시 근본 원인을 구명할 수 있는 기초자료로 이용하거나 조사료의 연중 안정 생산, 공급에 도움을 주고자 수행하였다. 2015년도 전국 동계 사료작물의 월동 후 생육 상황은 전반적으로 저조하였는데, 월동률 분포에 따른 지역별 분포 상황을 살펴보면 월동률 80% 이상인 지역이 66%, 월동률 79~50% 분포지역이 24.9%, 50%미만 지역이 9.1%의 지역별 분포를 나타내어 전체적으로는 79%의 월동률을 나타냈다. 월동 후 월동률 및 피복률은 배수로가 설치된 논에서는 각각 83%와 80%로서 양호했으나 배수로가 설치 안 된 습한 논에서는 각각 67%와 66%로 낮은 경향을 나타냈다. 전국 동계작물 조사료 생산량은 강원, 충북, 충남, 경남, 전남지역은 10~15%, 경기, 경북, 전북지역은 약 30%의 수량감소가 예상되어 전국적으로 약 19%의 수량 감소가 예상되었다. In order to identify the causes of various problems related to forage crop growth, such as winter survival, coldness, rainfall, drought etc., and to provide basic data for the stable production and supply of forage year round, we performed a growth survey after the wintering of winter forage crops grown from mid-Sep. 2014 to late-Feb. 2015. The growth of winter forage crops after wintering in the country was generally bad. As shown in the regional distribution in the country, regions with 80% or higher winter survival rates comprised 66%, regions with 79 to 50% winter survival comprised 24.9% and regions with less than 50% winter survival comprised 9.1%. In conclusion, the average winter survival rate was 79% in the country. Winter survival rate and coverage rate after the wintering of winter forage crops under installed drain channels in paddy fields were good at 83% and 80%, respectively. However, the rates without installed drain channels were bad at 67% and 66%, respectively. It was predicted that the crop production of winter forage crops was reduced by 10-15% in Gangwon, Chungbuk, Chungnam, Gyeongnam and Jeonnam regions, reduced by 30% in Gyeonggi, Gyeongbuk and Jeonbuk regions and reduced overall by approximately 19% nationwide.

2014 / 2015년 이탈리안 라이그라스 (Lolium multiflorum Lam.)의 벼 입모 중 파종 재배시 생육저해 요인 분석

김영진,최기춘,이상학,정종성,박형수,김기용,지희정,이상훈,최기준,김원호,Kim, Young-Jin,Choi, Ki-Choon,Lee, Sang-Hak,Jung, Jeong-Sung,Park, Hyung-Soo,Kim, Ki-Yong,Ji, Hee-Chung,Lee, Sang-Hoon,Choi, Gi-Jun,Kim, Won-Ho 한국초지조사료학회 2016 한국초지조사료학회지 Vol.36 No.1

2014/2015년도 전국 동계사료작물, 특히 벼 입모 중 파종했던 이탈리안 라이그라스의 월동 후 생육 상황은 매우 저조했다. 이탈리안 라이그라스의 생육저해 요인을 찾기 위해 충남 논산지역의 생육상황과 인근 대전지역의 기상을 분석한 결과는 다음과 같다. 월동 후인 2015년 2월 8일과 9일의 최저기온과 최대순간풍속은 각각 $-8.8^{\circ}C$와 10.7 m/s, $-12.4^{\circ}C$와 9.6 m/s로서 소낙눈을 동반한 강풍이 불면서 온도도 매우 낮았는데, 이는 토양 과습으로 토양 표면에 노출되어 있던 이탈리안 라이그라스의 뿌리 생장에 매우 불리하게 작용하였다. 연이어 2월 12, 13, 14일에도 각각 $-4.1^{\circ}C$와 11.6 m/s, $-5.6^{\circ}C$와 10.3 m/s, $-4.7^{\circ}C$와 7.5 m/s의 저온과 강풍피해를 받았다. 더욱이 1월부터 지속된 가뭄현상으로 건조상태가 지속되다가 2월 15, 16일에 19 mm의 강우가 내렸으나 생육을 회복하기엔 역부족이었다. 2월 26, 27, 28일에도 $-1.8^{\circ}C$와 13.7 m/s, $-3.5^{\circ}C$와 10.6 m/s, $-4.1^{\circ}C$와 6.8 m/s의 저온과 강풍피해가 있었다. 3월 3일까지 고사개체가 59% 이상으로 많았으며 3월 4일부터 12일까지 9일간의 저온과 강풍은 이탈리안 라이그라스를 생육 회복불능상태로 몰고 갔다. 따라서 2014/2015년의 이탈리안 라이그라스 수량감소의 주원인은 다음과 같이 분석되었다. 2014년 10월, 11월의 강우로 인하여 벼 수확 후 볏짚수거가 지연되어 볏짚 밑에 눌려 연약하게 자란 개체가 월동 후 저온에 고사되었다. 배수로 미설치로 논 토양 표면의 수분이 과다하여 뿌리를 땅속으로 내리지 못한 채 월동하게 된 이탈리안 라이그라스가 봄에 서릿발 및 가뭄과 강풍을 동반한 저온피해를 받아 뿌리가 말라버려 작물이 고사되어 버리는 상황이 발생되었다. The growth of Italian ryegrass (IRG) after wintering was very low in 2015 when IRG was broadcasted under growing rice in fall of 2014. To determine growth inhibitory factors of IRG, we examined the growth conditions of IRG in Nonsan region and meteorological conditions in Daejeon nearby Nonsan. Minimum temperature and maximum instantaneous wind speed on Feb. $8^{th}$ and $9^{th}$ of 2015 after wintering of IRG were $8.8^{\circ}C$, 10.7 m/s and $12.4^{\circ}C$, 9.6m/s, respectively. Air temperature was suddenly dropped due to strong wind with snow showers, which had unfavorable effect on root growth of IRG exposed at the soil surface. The minimum temperature and maximum instantaneous wind speed on Feb. $12^{th}$, $13^{th}$, and $14^{th}$ of 2015 were $4.1^{\circ}C$, 11.6 m/s, $-5.6^{\circ}C$, 10.3 m/s, and $-4.7^{\circ}C$, 7.5 m/s, respectively. The growth circumstance of IRG was not good because soil was dried due to drought continued from January. The minimum temperature and maximum instantaneous wind speed on Feb. $26^{th}$, $27^{th}$, and $28^{th}$ of 2015 were $1.8^{\circ}C$, 13.7 m/s, $-3.5^{\circ}C$, 10.6 m/s, and $4.1^{\circ}C$, 6.8 m/s, respectively. The number of wilting of IRG was more than 59% until Mar. $3^{rd}$ of 2015. IRG faced irreparable environment (low minimum temperatures and extreme instantaneous wind speeds) for 9 days from Mar. $4^{th}$ to Mar. $12^{th}$ of 2015. The main reason for the decrease of IRG productivity was collection delay of rice straw after rice harvest because there was continuous rain between Oct. and Nov. of 2014. For this reason, weakly grown IRG under rice straw was withered after wintering. IRG was withered by frost heaving, drought, and instantaneous wind speed in the spring. Furthermore, the root of IRG was damaged while growing in excess moisture in the surface of paddy soil during the winter season due to rain.

Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells

김영진,송희종,김종면,강명대,윤창용,김태중,Kim, Young-jin,Song, Hee-jong,Kim, Jong-myeon,Kang, Myeong-dai,Yoon, Chang-yong,Kim, Tae-joong The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.2

돼지(n=22) 순환혈액내 단핵세포(PB-MNCs)중 T 및 B 림프구를 정량하고자 rosette 형성기법을 적용하였다. T 림프구는 여러 농도의 2-aminoethylisothiouronium bromide(Aet)나 dextran(Dex) 용액 또는 Aet와 Dex로 조합 처리된 면양(SRBC) 및 재래산양(GRBC)의 적혈구를 사용한 E-rosette 법으로, B 림프구는 erythrocyeantibody(EA) 및 erythrocyte-antibody-complement(EAC) rosette 법으로 각각 정량하였다. 한편 rosette 형성세포의 냉장정치시간에 따른 판독결과를 비교하여 적정판독시간대를 구명한 바 아래의 결과를 얻었다. 1. PB-MNCs와 SRBC/GRBC와의 자연 rosette형성을(RFR)은$ 32.9{\pm}7.9%/31.3{\pm}9.4%$인데 비하여 Aet 또는 Dex 처리군에서는 모두 증가되었고 특히 0.15M Aet$(40.2{\pm}4.6%/37.0{\pm}3.3%)$ 및 8% Dex$(71.5{\pm}4.5%/69.9{\pm}5.8%)$ 처리군에서 각각 높게 나타났다. 한편 0.1M Aet처리후 8% Dex 첨가군에서는 $67.8{\pm}7.4%$ 및 $69.8{\pm}8.5%$로 나타나 복잡한 Aet와 Dex를 복합처리하기 보다는 8% Dex 단독처리가 간편함을 알 수 있었다. 2. Rosette 형성세포의 냉장보관중 최적판독시간대는 10~20시간의 범위이었다. 3. EA 및 EAC의 RFR은 SRBC의 경우 $39.1{\pm}10.2%$, $27.6{\pm}7.0%$, GRBC의 경우 $32.6{\pm}6.1%$, $21.0{\pm}3.2%$로 나타났다. 이러한 결과는 돼지 PB-MNCs내의 T 및 B 림프구를 rosette 형성법으로 분리할 수 있으며 rosette assay에서 SRBC 뿐아니라 GRBC의 이용이 가능함을 제시한다. To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{\pm}7.9%$ and $31.3{\pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{\pm}7.4%$ and $69.8{\pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{\pm}10.2%$ and $32.6{\pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{\pm}7.0%$ arld $21.0{\pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs.

만성 단독 후방십자인대 파열에서 자가 슬괵건과 후-후 삼각술기를 이용한 만성 후방십자인대 보강술

김영진,채수욱,최병산,김종윤,이황용,한창완,한수연,Kim, Yeung Jin,Chae, Soo Uk,Choi, Byong San,Kim, Jong Yun,Lee, Hwang Yong,Han, Chang Wan,Han, Su Hyoun 대한관절경학회 2013 대한관절경학회지 Vol.17 No.1

목적: 슬관절의 후방십자인대 파열 시 잔존하는 절단단 또는 이완된 인대를 유지하면서 관절경하 후-후 삼각기법과 단일절개 횡경골 접근법을 이용하여 4겹의 자가 슬괵건을 사용해 손상된 후방십자인대 보강술을 시행한 결과를 후향적으로 분석해 보고자 하였다. 대상 및 방법: 2002년 1월부터 2009년 12월까지 후방십자인대 파열로 진단되어서 4겹의 자가 슬괵건과 후-후 삼각기법과 단일절개 횡경골 접근법을 이용한 보강술을 시행한 환자 중에서 최소 2년 이상 추시가 가능하였던 32예를 대상으로 하였다. 평균 추시는 5년 5개월(범위: 2-7.9)이었다. 수술 시 연령은 평균 35.2세이었다. 운동 가능 범위, 후방 전위 검사, Telos stress 방사선 검사, Lysholm knee score, Tegner activity score, International Knee Documentation Committee (IKDC)의 판정 기준을 이용하여서 술후 기능 평가, 2차적 관절경 검사 등을 시행하여 주관적 및 객관적 지표로 삼았다. 결과: 최종 추시 시에서 후방 스트레스 방사선 사진상 통계학적으로 의미 있는 감소 소견이 관찰되었다(p<0.05). 최종 추시에서 IKDC grade는 Group A가 18예, Group B가 11예, Group C가 3예이었고, Group D는 한 예도 없었으며, 각각 통계학적 의미가 있는 호전을 보였다(p<0.05). 결론: 만성 단독 후방십자인대의 파열에서 관절경하 후-후 삼각기법과 단일절개 횡경골 접근법을 이용하여 4겹의 자가 슬괵건을 사용해 손상된 후방십자인대 보강술은 양호한 슬관절의 안정성과 기능 회복을 얻을 수 있는 좋은 수술 방법으로 사료된다. Purpose: To evaluate the outcome of arthroscopic posterior cruciate ligament (PCL) augmentation using an autogenous hamstring tendon graft and the posterior-posterior triangulation technique with preservation of ligament remnant or elongated ligament. Materials and Methods: From January 2002 to December 2009, we performed PCL augmentation using an autogenous hamstring tendon graft and the posterior-posterior triangulation technique in 32 patients. The mean age was 35.2 years. Twenty two cases were male and 10 cases were female. Average follow-up period was 5 years and 5 months (range: 2-7.9). Subjective and objective parameters were utilized in analyses, such as the mean range of motion, post. drawer test, Lysholm knee score, Tegner activity score, International Knee Documentation Cominittee (IKDC) grade, and second look arthroscopic examination. Results: At last follow up posterior displacement by the Telos stress test decreased from $10.8{\pm}5.1\;mm$ to $2.8{\pm}3.7\;mm$ (p<0.05). The final Lysholm knee score improved from $60.4{\pm}5.8$ to $84.6{\pm}4.8$. Tegner activity score improved from 3.2 to 4.8. The final IKDC grade was A in 18, B in 11, C in 3. Postoperative Lysholm knee score, IKDC grades, Tegner activity scale, and posterior displacement demonstrated statistically significant improvement compared to the preoperative state (p<0.05). Conclusion: Arthroscopic PCL reconstruction using an autogenous hamstring tendon with preservation of ligament remnant showed a good clinical results and posterior stability.

유체 전산모사를 통한 직접 메탄올 연료전지의 시뮬레이션

김영진,이태희,오인환,홍성안,김혁년,하흥용,Kim, Young-Jin,Lee, Tae-Hee,Oh, In-Hwan,Hong, Seong-An,Kim, Huk-Nyun,Ha, Heung-Yong 한국전기화학회 2003 한국전기화학회지 Vol.6 No.1

상용 유체 전산모사 코드인 FLUENT ver.6.0을 이용하여 직접 메탄을 연료전지의 전기화학적 해석 및 유로 내에서의 유체의 유동특성을 분석하였다 본 전산모사를 통해 유로 내의 유속, 압력, 온도, 농도 및 전류밀도 분포에 대한 다양한 정보를 얻을 수 있었으며, 유로 디자인에 따른 반응물 및 생성물의 유동에 대한 정보로부터 최적의 유로 형태를 결정할 수 있었다. 이와 같은 전산모사 방법을 사용하면 직접 메탄을 연료전지의 전극과 분리판 유로의 구조를 최적화하는데 매우 유리하다. A numerical analysis of electrochemical reaction and dynamics of the fluid flow in the channels of a DMFC separator was carried out by using a commercial Computational Fluid Dynamics(CFD) code fluent(ver.6.0). From the simulation work, many valuable informations were obtained in terms of distributions of velocity, pressure, temperature, concentration and current density over the flow field. And it was possible to optimize the flow field structure by using the simulation results. The simulation work using the Cm code was found very helpful in analysing the phenomena occurring in the fuel cell and optimizing the structures of electrodes and flow field.

KSTAR장치의 TF MPS 냉각수시스템 시운전 결과

김영진(Young-Jin Kim),김상태(Sang-Tae Kim),임동석(Dong-Seok Im),정남용(Nam-Yong Jung),김동진(Dong-Jin Kim),최재훈(Jae-Hoon Choi),이동근(Dong-Keun Lee),김양수(Yang-Su Kim),박주식(Joo-Shik Park),이용운(Yong-Woon Lee) 대한설비공학회 2008 대한설비공학회 학술발표대회논문집 Vol.2008 No.2

The toroidal field magnet power supply (TF MPS) for the KSTAR was constructed in August, 2007 and started the operation for the commissioning in March, 2008. The main role of the TF MPS is to supply the electric power to the TF magnet of the KSTAR. The water cooling components of the TF MPS are 16 stacks, busbar of 70 meters. For the cooling of the TF MPS, the D·I water cooling system was designed and installed. The water cooling system consists of several pumps, heat exchangers, D·I water polishing system and so on. The water cooling system for the TF MPS was tested under the 15 ㎄ current charging condition. In this paper be discussed about the system performance and other parameters.

소규모 농산가공시설 배출수 처리시설 개선방안

김영진(Young jin Kim),전종길(Jong gil Jeon),김민영(Min young Kim),최용훈(Yong hun Choi) 한국농촌지도학회 2016 농촌지도와 개발 Vol.23 No.4

This study examined the regulation status on wastewater treatment in agro-food processing and a case study on effluent treatment m ethods was carried out to evaluate any change after the mitigation of regulation. First, in order to clarify the area of investigation, the definition of small-scale agro-processing facilities was reviewed through literature survey and local government ordinance. The current law were separately analyzed into four areas; effluent treatment facilities, development of agro-processing industries, land use and food processes equipment. The exclusion clauses on wastewater discharging facility in the enforcement regulation were defined in detail, which can be served in practice. Site survey, after the questionnaire survey of the person in charge of the local unit, was carried out. As the result, this survey confirmed the positive effects of the deregulation on promoting sewer system service in rural areas, introducing the new processing construction and so on. In addition, it was found that some matters to be considered to determine whether to introduce wastewater treatment plan for public food processing facilities.

슬관절 전 치환술 후 심부 정맥 현전증의 발생에 대해 D-dimer 검사의 유용성

김영진(Young-Jin Kim),허창익(Chang-Ich Hur),송은규(Eun-Kyoo Song),선종근(Jong-Keun Seon),박상진(Sang-Jin Park),조성범(Seong-Beom Cho),조용진(Yong Jin Cho) 대한정형외과학회 2007 대한정형외과학회지 Vol.42 No.4

목적: 슬관절 전 치환술 후에 발생하는 증상이 있는 심부 정맥 혈전증의 빈도를 알아보고 D-dimer 검사의 유용성에 대해 알아보고자 한다. 대상 및 방법: 2006년 4월부터 2006년 7월까지 퇴행성 슬관절염으로 슬관절 전 치환술을 시행한 140명의 환자를 전향적으로 조사하였다. 알려진 위험인자들에 대해 평가하였으며, D-dimer 검사는 술 전 및 술 후 1, 7, 14일째 시행하였다. 술 후 7일째 CT 정맥 조영술을 시행하여 혈전증 유무를 조사하였다. 결과: 총 48명 (34%)에서 심부 정맥 혈전증이 발생하였으며, 이 중 증상을 호소한 경우는 5명(10.4%)이었다. 위험인자들 중 심부 정맥 혈전증이 발생한 군과 발생하지 않은 군 간의 유의한 차이를 보이는 경우는 비만도 뿐이었으며, D-dimer 검사상 두 군 간의 유의한 차이를 보이는 경우는 술 후 7일째였으며 각각 평균 4.90 ㎎/L와 3.12 ㎎/L였다. 결론: 증상을 보이는 심부 정맥 혈전증의 빈도는 높지 않았다. 비만도, 임상증상 및 술 후 7일째 측정한 D-dimer 검사를 심부 정맥 혈전증의 선별검사로 이용할 수 있으리라 사료된다. Purpose: To investigate the prevalence of deep vein thrombosis (DVT) after Total knee arthroplasty (TKA) and evaluate the availability of D-dimer test for the diagnosis of DVT after TKA. Materials and Methods: From April 2006 to July 2006, 140 consecutive patients, who had degenerative osteoarthritic knees and underwent primary total knee arthroplasty, were enrolled in this study. General and hematological risk factors were compared between DVT positive and negative patients. D-dimer level was checked preoperatively, and at 1, 7 and 14 days after surgery. A diagnosis of DVT was established by computed tomography venography. Results: DVT was found in 48 (34%) patients and only 5 (10.4%) showed symptoms of DVT. Regarding the risk factors, only obesity was significantly associated with an increased incidence of DVT (p=0.049). Seven days after surgery, the average D-dimer level in the DVT positive and negative patients was 4.90 mg/L and 3.12 mg/L, respectively (p=0.000). Conclusion: In our study patients, the incidence of symptomatic DVT was lower than in the Western population. We recommend obesity, clinical symptoms and D-dimer value at 7 days after surgery as a valuable screening tests for detecting DVT.