Ultrastructural Study on the cleistothecium development in Emericella nidulans

(Wei Jie Fu),(Kwon Sang Yoon),(K . D . Sohn) 한국균학회 2001 Proceedings of the Fifth Korea-China Joint Symposi Vol.- No.-

Component Prototyping for the China Spallation Neutron Source Project

Jie Wei,Yanwei Chen,Yunlong Chi,Changdong Deng,Haiyi Dong,Shinian Fu,Wei He,Kaixi Huang,Wen Kang,Jian Li,Huafu Ouyang,Huamin Qu,Caitu Shi,Hong Sun,Jingyu Tang,Juzhou Tao,Sheng Wang,Zhongxiong Xu,Xueju 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.5

The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years. The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years.

Clinical Value of Real Time Elastography in Patients with Unexplained Cervical Lymphadenopathy: Quantitative Evaluation

Fu, Ying,Shi, Yun-Fei,Yan, Kun,Wang, Yan-Jie,Yang, Wei,Feng, Guo-Shuang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Ultrasonography is non-invasive and can give useful clues in the diagnosis of cervical lymphadenopathy, However, differential diagnosis is difficult in some situations even combined with color Doppler imaging. The present study was conducted to evaluate the clinical value of real time elastography in patients with unexplained cervical lymphadenopathy using a quantitative method. From May 2011 to February 2012, 39 enlarged lymph nodes from 39 patients with unexplained cervical lymphadenopathy were assessed. All the patients were examined by both B-mode ultrasound, color Doppler flow imaging and elastography. The method of analyzing elasto-graphic data was the calculation of the 10 parametres ("mean", "sd", "area%", "com", "kur", "ske", "con", "ent", "idm", "asm") offered by the software integrated into the Hitachi system. The findings were then correlated with the definitive tissue diagnosis obtained by lymph node dissection or biopsy. Final histology revealed 10 cases of metastatic lymph nodes, 11 cases of lymphoma, 12 cases of tuberculosis and 6 cases of nonspecific lymphadenitis. The significant distinguishing features for conventional ultrasound were the maximum short diameter (p=0.007) and absent of echogenic hilum (p=0.0293). The diagnostic accuracy was 43.6% (17/39 cases) and there were 17 patients with equivocal diagnosis. For elastography, "mean" (p=0.003), "area%" (p=0.009), "kurt" (p=0.0291), "skew" (p=0.014) and "cont" (p=0.012) demonstrated significant differences between groups. With 9 of the 17 patients with previous equivocal diagnoses (52.9%) definite and correct diagnoses could be obtained. The diagnostic accuracy for conventional ultrasound combined elastography was 69.2% (27/39 cases). There were differences in the diagnostic sensitivity of the two methods (p=0.0224). Ultrasound combined with elastography demonstrated higher rates of conclusive and accurate diagnoses in patients with unexplained cervical lymphadenopathy than conventional ultrasound. The quantitative program showed good correlation with the pathology of different lymph node diseases.

Efficacy and Safety of Intravenous Urapidil for Older Hypertensive Patients with Acute Heart Failure: A Multicenter Randomized Controlled Trial

Wei Yang,Qi Hua,Yu-Jie Zhou,Yan Fu,Jian Qin,Shu Qin,Xiao-Min Chen,Jin-Cheng Guo,De-Zhao Wang,Hong Zhan,Jing Li,Jing-Yu He 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.1

Purpose: Urapidil is putatively effective for patients with hypertension and acute heart failure, although randomized controlled trials thereon are lacking. We investigated the efficacy and safety of intravenous urapidil relative to that of nitroglycerin in older patients with hypertension and heart failure in a randomized controlled trial. Materials and Methods: Patients (>60 y) with hypertension and heart failure were randomly assigned to receive intravenous urapidil (n=89) or nitroglycerin (n=91) for 7 days. Hemodynamic parameters, cardiac function, and safety outcomes were compared. Results: Patients in the urapidil group had significantly lower mean systolic blood pressure (110.1±6.5 mm Hg) than those given nitroglycerin (126.4±8.1 mm Hg, p=0.022), without changes in heart rate. Urapidil was associated with improved cardiac function as reflected by lower N terminal-pro B type natriuretic peptide after 7 days (3311.4±546.1 ng/mL vs. 4879.1±325.7 ng/mL, p=0.027) and improved left ventricular ejection fraction (62.2±3.4% vs. 51.0±2.4%, p=0.032). Patients given urapidil had fewer associatedadverse events, specifically headache (p=0.025) and tachycardia (p=0.004). The one-month rehospitalization and all-cause mortality rates were similar. Conclusion: Intravenous administration of urapidil, compared with nitroglycerin, was associated with better control of blood pressure and preserved cardiac function, as well as fewer adverse events, for elderly patients with hypertension and acute heart failure.

In‑situ growth of ZIF‑8/CP with ultra‑high adsorption capacity for removing Malachite green from water

Jie Lou,Qiuping Fu,Lei Yu,Hui Yuan,Jie Zhao,Xinde Wei,Tong Wang,Changli Mo 한국탄소학회 2023 Carbon Letters Vol.33 No.7

An environmentally friendly and low-cost chitosan-containing polysaccharide (CP) composite ZIF-8/CP was designed and prepared based on the difficulty of separating the traditional adsorbent from the water phase. ZIF-8/CP was synthesized through in-situ growth approach. The physical, chemical and structure properties of ZIF-8/CP were determined through a series of characterization methods, including SEM, FT-IR and PXRD. The effects of touch time, pH, temperature, and coexisting ions on adsorption were assessed. In addition, kinetics, isotherms of adsorption and thermodynamics were examined. The data of isotherms for adsorption indicated that the adsorption of ZIF-8/CP on MG was similar to the Langmuir model, with a maximum adsorption capacity of 1428.57 mg/g. Moreover, the kinetic parameters were consistent with the pseudo- 2nd-order equation. Thermodynamic studies (ΔG < 0, ΔH > 0) demonstrated a heat-absorbing and spontaneous adsorption process. Our study reveals that ZIF-8/CP has good adsorption properties and environmental properties.

Effect of Crystal Structure and Hydrogen Bond of Thermoplastic Oxidized Starch on Manufacturing of Starch-Based Biomass Composite

Chuan-Wei Zhang,Fang-Yi Li,Jianfeng Li,Qi Xie,Jie Xu,An-Fu Guo,Cheng-Zhao Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.5 No.3

Starch-based biomass composites were prepared with starch and plant fiber. In order to improve the properties of the compos ites, oxidized starches (OS) were prepared using hydroxyl peroxide as oxidizer, then glycerol was added into OS as plasticizer to produce thermoplastic oxidized starches (TPOS). Crystal structure, hydrogen bonds of TPOS were researched to gain comprehensive views on the mechanism of the property differences between the starch-based composites with native starch, OS and TPOS. The changes of crystal structure were analyzed by X-ray diffraction analysis. Starch intermolecular hydroxyl changes were investigated using the method of infrared spectrum analysis. It was found that the new hydrogen bonds were formed between the plasticizer, oxidizer and starch, and the starch intermolecular bonding force was weakened. The structure of starch crystallization was destructed in TPOS. Differential scanning calorimetry showed that cross reaction with oxidization and plasticization changed the crystal structure, glass transition process was not observed in TPOS. The tensile and compressive strengths of composite based on TPOS were improved greatly and mechanical properties were not weakened obviously with water content increased. All the results indicated that TPOS could improve the properties of starch-based composites.

Developmental Exposure to Di-(2-ethylhexyl) Phthalate Induces Cerebellar Granule Cell Apoptosis via the PI3K/AKT Signaling Pathway

Yuanyuan Fu,Jing Dong,Jianan Wang,Mingdan You,Lingling Wei,Hui Fu,Yuan Wang,Jie Chen 한국뇌신경과학회 2018 Experimental Neurobiology Vol.27 No.6

Di-(2-ethylhexyl) phthalate (DEHP) is an ubiquitous environmental contaminant because of its extensive use in plastics and its persistence. As an environmental endocrine disruptor, it is suspected to interfere with neurodevelopment in people. However, evidence of the effects of maternal DEHP exposure on cerebellar development in offspring is scarce. The objective of this study was to investigate maternal exposure to DEHP and its effect on apoptosis of cerebellar granule cells (CGCs) and related mechanisms. Pregnant Wistar rats were administrated DEHP (0, 30, 300 and 750 mg/kg/d) by gavage from gestational day (GD) 0 to postnatal day (PN) 21. Primary CGCs were also exposed to mono-(2-ethylhexyl) phthalate (MEHP), the main metabolite of DEHP, for 24 h with concentrations of 0, 25, 100 and 250 μM. The CGCs of male offspring from 300 and 750 mg/kg/d DEHP exposure groups showed significantly increased apoptosis. In addition, the PI3K/AKT signaling pathway was inhibited in the male offspring of the 300 and 750 mg/ kg/d DEHP exposure groups. However, effects on female pups were not obvious. Apoptosis was also elevated and the PI3K/AKT signaling pathway was inhibited after primary CGCs were exposed to MEHP. Furthermore, apoptosis was reduced after treatment with the PI3K/AKT signaling pathway activator, insulin-like growth factor (IGF) 1, and increased after treatment with LY294002, an inhibitor of the PI3K/AKT signaling pathway. These results suggested that maternal DEHP exposure induced apoptosis in the CGCs of male pups via the PI3K/AKT signaling pathway, and the apoptosis could be rescued by IGF1 and aggravated by LY294002.

3D-QSAR and Molecular Docking Studies on Benzotriazoles as Antiproliferative Agents and Histone Deacetylase Inhibitors

Li, Xiaolin,Fu, Jie,Shi, Wei,Luo, Yin,Zhang, Xiaowei,Zhu, Hailiang,Yu, Hongxia Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

Benzotriazole is an important synthetic auxiliary for potential clinical applications. A series of benzotriazoles as potential antiproliferative agents by inhibiting histone deacetylase (HDAC) were recently reported. Three-dimensional quantitative structure-activity relationship (3D-QSAR), including comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA), were performed to elucidate the 3D structural features required for the antiproliferative activity. The results of both ligand-based CoMFA model ($q^2=0.647$, $r^2=0.968$, ${r^2}_{pred}=0.687$) and CoMSIA model ($q^2=0.685$, $r^2=0.928$, ${r^2}_{pred}=0.555$) demonstrated the highly statistical significance and good predictive ability. The results generated from CoMFA and CoMSIA provided important information about the structural characteristics influence inhibitory potency. In addition, docking analysis was applied to clarify the binding modes between the ligands and the receptor HDAC. The information obtained from this study could provide some instructions for the further development of potent antiproliferative agents and HDAC inhibitors.

3D-QSAR and Molecular Docking Studies on Benzotriazoles as Antiproliferative Agents and Histone Deacetylase Inhibitors

Xiaolin Li,Jie Fu,Wei Shi,Yin Luo,Xiaowei Zhang,Hailiang Zhu,Hongxia Yu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

Benzotriazole is an important synthetic auxiliary for potential clinical applications. A series of benzotriazoles as potential antiproliferative agents by inhibiting histone deacetylase (HDAC) were recently reported. Threedimensional quantitative structure-activity relationship (3D-QSAR), including comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA), were performed to elucidate the 3D structural features required for the antiproliferative activity. The results of both ligand-based CoMFA model (q2 = 0.647, r2 = 0.968, r2 pred = 0.687) and CoMSIA model (q2 = 0.685, r2 = 0.928, r2 pred = 0.555) demonstrated the highly statistical significance and good predictive ability. The results generated from CoMFA and CoMSIA provided important information about the structural characteristics influence inhibitory potency. In addition, docking analysis was applied to clarify the binding modes between the ligands and the receptor HDAC. The information obtained from this study could provide some instructions for the further development of potent antiproliferative agents and HDAC inhibitors.

Single Nucleotide Polymorphisms of NLRP12 Gene and Association with Non-specific Digestive Disorder in Rabbit

Liu, Yun-Fu,Zhang, Gong-Wei,Xiao, Zheng-Long,Yang, Yu,Deng, Xiao-Song,Chen, Shi-Yi,Wang, Jie,Lai, Song-Jia Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8

The NLRP12 (NLR family, pyrin domain containing 12) serves as a suppressor factor in the inflammatory response and protects the host against inflammation-induced damage. In the present study, we aimed to study the polymorphisms of NLRP12 gene and its association with susceptibility to non-specific digestive disorder (NSDD) in rabbits. We re-sequenced the entire coding region of the rabbit NLRP12 gene and detected a total of 19 SNPs containing 14 synonymous and five non-synonymous variations. Among them, the coding SNP (c.1682A>G), which would carry a potential functional implication, was subsequently subjected to genotyping for case-control association study (272 cases and 267 controls). The results revealed that allele A was significantly protective against NSDD with an odds ratio value of 0.884 (95% confidence interval, 0.788 to 0.993; p = 0.038). We also experimentally induced NSDD in growing rabbits by feeding a fibre-deficient diet and subsequently investigated NLRP12 mRNA expression. The mRNA expression of NLRP12 in healthy status was significantly higher than that in severe NSDD (p = 0.0016). The highest expression was observed in individuals carrying the protective genotype AA (p = 0.0108). These results suggested that NLRP12 was significantly associated with the NSDD in rabbits. However, the precise molecular mechanism of NLRP12 involving in the development of rabbit NSDD requires further research.