Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene

Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.

Investment Climate Analysis of China and South Korea: Based on Grading Method

Jing LI(Jing LI),Xin Yu XU(Xin Yu XU),Jie XU(Jie XU),Shuai SU(Shuai SU),Fan ZHANG(Fan ZHANG) 한국4차산업학회 2022 4차산업연구 Vol.2 No.2

Purpose - This study analyzes the investment environment of South Korea by using the rating scale of Robert B Stobaugh, Jr, and draws conclusion implication. Research design, data, and methodology - The study conducted a survey on according to the political stability, capital repatriation, foreign ownership allowed, discrimination and controls, foreign vs domestic businesses, currency stability, willingness to grant tariff protection, availability of local capital, and annual inflation for last 5 years. The score of these eight aspects will be given based on the current situation in South Korea and the sum of the scores will be calculated. Result - China-Korea economic and trade relations are in a stage of transformation and upgrading, and the level of economic and trade cooperation in various fields is reaching a new level. It is hoped that Chinese enterprises will grasp business opportunities, strengthen research and analysis of the Korea market and achieve mutually beneficial cooperation. Conclusion - The investment environment of South Korea is superior according to the political stability, capital repatriation, foreign ownership allowed, discrimination and controls, foreign vs domestic businesses, currency stability, willingness to grant tariff protection, availability of local capital, and annual inflation for last 5 years.

Circular RNA CREBBP modulates cartilage degradation by activating the Smad1/5 pathway through the TGFβ2/ALK1 axis

Xu Yiyang,Mao Guping,Long Dianbo,Deng Zengfa,Xin Ruobin,Zhang Ziji,Xue Ting,Liao Weiming,Xu Jie,Kang Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Osteoarthritis, characterized by articular cartilage degradation, is the leading cause of chronic disability in older adults. Studies have indicated that circular RNAs are crucial regulators of chondrocyte development and are involved in the progression of osteoarthritis. In this study, we investigated the function and mechanism of a circular RNA and its potential for osteoarthritis therapy. The expression levels of circCREBBP, screened by circular RNA sequencing during chondrogenic differentiation in adipose tissue-derived stem cells, and TGFβ2 were significantly increased in the cartilage of patients with osteoarthritis and IL-1β-induced chondrocytes. circCREBBP knockdown increased anabolism in the extracellular matrix and inhibited chondrocyte degeneration, whereas circCREBBP overexpression led to the opposite effects. Luciferase reporter assays, rescue experiments, RNA immunoprecipitation, and RNA pulldown assays confirmed that circCREBBP upregulated TGFβ2 expression by sponging miR-1208, resulting in significantly enhanced phosphorylation of Smad1/5 in chondrocytes. Moreover, intra-articular injection of adeno-associated virus-sh-circCrebbp alleviated osteoarthritis in a mouse model of destabilization of the medial meniscus. Our findings reveal a critical role for circCREBBP in the progression of osteoarthritis and provide a potential target for osteoarthritis therapy.

Investigation of random fatigue life prediction based on artificial neural network

Jie Xu,Chongyang Liu,Xingzhi Huang,Yaolei Zhang,Haibo Zhou,Hehuan Lian 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.46 No.3

Time domain method and frequency domain method are commonly used in the current fatigue life calculation theory. The time domain method has complicated procedures and needs a large amount of calculation, while the frequency domain method has poor applicability to different materials and different spectrum, and improper selection of spectrum model will lead to large errors. Considering that artificial neural network has strong ability of nonlinear mapping and generalization, this paper applied this technique to random fatigue life prediction, and the effect of average stress was taken into account, thereby achieving more accurate prediction result of random fatigue life.

Hyperoside attenuates pyrrolizidine alkaloids-induced liver injury by ameliorating TFEB-mediated mitochondrial dysfunction

Jie Xu,Aizhen Xiong,Xunjiang Wang,Xing Yan,Yilin Chen,Xuanling Ye,Zhengtao Wang,Lili Ding,Li Yang 대한약학회 2023 Archives of Pharmacal Research Vol.46 No.8

Pyrrolizidine alkaloids (PAs) are potent hepatotoxins that can cause liver damage. Hyperoside (Hyp), a natural flavonoid, can be extracted from medicinal plants. Hyp displays hepatoprotective activity in various liver diseases. However, the potential effect and mechanism of action of Hyp in ameliorating PA-induced liver injury remain obscure. This study aimed to explore the protective effect of Hyp against PA-induced hepatotoxicity and its underlying mechanism. We established an in vitro model of PAs in mouse primary hepatocytes and developed a mouse model of acute PA toxicity to investigate the protective effect of Hyp. We found that Hyp notably attenuated PA-induced hepatotoxicity. RNA-sequencing showed that the beneficial effect of Hyp against PA-induced hepatotoxicity was associated with the transcription factor EB (TFEB)-peroxisome proliferator-activated receptor-γ coactivator-1-α (PGC1α) pathway. Our results confirmed that both the autophagy-lysosomal pathway and mitochondrial biogenesis were induced by Hyp through TFEB nuclear translocation in PA-induced liver injury. Furthermore, we demonstrated that activation of the mechanistic target of rapamycin complex 1 (mTORC1) by MHY 1485 decreased TFEB nuclear translocation and abrogated the protective effect of Hyp against PA-induced liver injury in mice. In contrast, inhibition of mTORC1 activity increased the level of TFEB and reduced hepatotoxicity induced by PAs in mouse livers. Likewise, Hyp-induced TFEB activation was validated in vitro. In conclusion, Hyp can activate the TFEB-mediated autophagy-lysosomal pathway and mitochondrial biogenesis through inhibition of mTORC1 activity, alleviating the liver injury induced by PAs, thus suggesting the potential value of Hyp in the treatment of PA-induced hepatotoxicity.

Effect of fermented biogas residue on growth performance, serum biochemical parameters, and meat quality in pigs

Xu, Xiang,Li, Lv-mu,Li, Bin,Guo, Wen-jie,Ding, Xiao-ling,Xu, Fa-zhi Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

Objective: This study investigated the effect of fermented biogas residue (FBR) of wheat on the performance, serum biochemical parameters, and meat quality in pigs. Methods: We selected 128 pigs (the mean initial body weight was $40.24{\pm}3.08kg$) and randomly allocated them to 4 groups (1 control group and 3 treatment groups) with 4 replicates per group and 8 pigs per pen in a randomized complete block design based on initial body weight and sex. The control group received a corn-soybean meal-based diet, the treatment group fed diets containing 5%, 10%, and 15% FBR, respectively (abbreviated as FBR5, FBR10, and FBR15, respectively). Every group received equivalent-energy and nitrogen diets. The test lasted 60 days and was divided into early and late stages. Blood and carcass samples were obtained on 60 d. Meat quality was collected from two pigs per pen. Results: During the late stage, the average daily feed intake and average daily gain of the treatment groups was greater than that of the control group (p<0.05). During the entire experiment, the average daily gain of the treatment groups was higher than that of the control group (p<0.05). Fermented biomass residue did not significantly affect serum biochemical parameters or meat quality, but did affect amino acid profiles in pork. The contents of Asp, Arg, Tyr, Phe, Leu, Thr, Ser, Lys, Pro, Ala, essential amino acids, non-essential amino acids, and total amino acids in pork of FBR5 and FBR10 were greater than those of the control group (p<0.05). Conclusion: These combined results suggest that feeding FBR could increase the average daily gain and average daily feed intake in pigs and the content of several flavor-promoting amino acids.

Highly stretchable polymer semiconductor films through the nanoconfinement effect

Xu, Jie,Wang, Sihong,Wang, Ging-Ji Nathan,Zhu, Chenxin,Luo, Shaochuan,Jin, Lihua,Gu, Xiaodan,Chen, Shucheng,Feig, Vivian R.,To, John W. F.,Rondeau-Gagné,, Simon,Park, Joonsuk,Schroeder, Bob C.,L American Association for the Advancement of Scienc 2017 Science Vol.355 No.6320

<P>Soft and conformable wearable electronics require stretchable semiconductors, but existing ones typically sacrifice charge transport mobility to achieve stretchability. We explore a concept based on the nanoconfinement of polymers to substantially improve the stretchability of polymer semiconductors, without affecting charge transport mobility. The increased polymer chain dynamics under nanoconfinement significantly reduces the modulus of the conjugated polymer and largely delays the onset of crack formation under strain. As a result, our fabricated semiconducting film can be stretched up to 100% strain without affecting mobility, retaining values comparable to that of amorphous silicon. The fully stretchable transistors exhibit high biaxial stretchability with minimal change in on current even when poked with a sharp object. We demonstrate a skinlike finger-wearable driver for a light-emitting diode.</P>

Anti-PUE Attack Base on Fractal Dimension in Spectrum Sensing

Xu Jie,Fu Shuang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.10

Secure problem has become a major concern in spectrum sensing. PUE attack is a common attack in spectrum sensing. To defend PUE attack, an anti-PUE attack method in spectrum sensing based on fractal dimension is proposed. It detects PUE attack by identifying the modulation type of the received signal using SVM classifier. Sevcik fractal dimension in frequency domain (SFDF) and Higuchi fractal dimension (HFD) of the received signal are adopted as the characteristics for classification by SVM classifier. So the task of anti-PUE attack can be carried out in spectrum sensing. Besides the parameters for spectrum sensing, i.e. SFDF and HFD, no other parameter is required to be calculated, which will decrease the calculation amount and calculation time. The Numerical results show that, the proposed method can effectively detect the PUE attack. When SNR is larger than 10 dB, its PUE detection probability can reach 1. Even when SNR is low to -10 dB, the PUE detection probability is larger than 0.97.

Synthesis and characterization of monodisperse yttrium aluminum garnet (YAG) micro-crystals with rhombic dodecahedron

Xu, Tao,Yuan, Rui,Xu, Peng-Cheng,Pan, Dong-Jie,Yang, Woochul,Guo, Hai-Bo,Shen, Yu-Fang,Hu, Jian-Feng,Zhang, Zhi-Jun,Zhao, Jing-Tai Elsevier 2018 Journal of alloys and compounds Vol.762 No.-

<P><B>Abstract</B></P> <P>Uniform, monodisperse yttrium aluminum garnet (YAG) rhombic dodecahedron micro-crystals were synthesized using coprecipitation and hydrothermal methods. The Na<SUB>2</SUB>SO<SUB>4</SUB> as a surfactant was helpful in improving the dispersity of the crystallites and forming well-faceted, micro-sized dodecahedral YAG crystallites. The effect of the reaction time and the solvent has also been studied. The crystal growth mechanism called dissolution/crystallization was determined by analyzing the experimental and simulated results. And the specific well-developed crystal facets in the {110} family have been demonstrated by the theoretical calculation. A higher integrated emission intensity can be achieved for the YAG:Ce crystallites by improving the phase purity, morphology with better developed dodecahedrons, and dispersion. Furthermore, a rather high luminous efficacy (LE = 104.14 lm/W) for the white LED fabricated using the YAG:Ce<SUP>3+</SUP> phosphor prepared in this work was realized. The correlation between the crystal growth, morphology and luminescence properties of YAG crystallites has been established, which exhibits a great importance for the synthesis and application of mono-dispersed YAG crystallites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform and monodisperse YAG rhombic dodecahedron micro-crystals were synthesized. </LI> <LI> The growth mechanism of YAG rhombic dodecahedron was studied through experimental and computational simulation. </LI> <LI> The correlation between the crystal growth, morphology and luminescence of YAG crystallites was established. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Numerical Simulation of Spray Flow in Injector For the Steamjet Engine

( Xu Sheng Shang ),( Jiang Hong Li ),( Ren Hou Zhao ),( Xu Ze Shang ),( Jie Hong Li ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-

In this paper, according to the work mechanism of SteamJet, With the certain small scaled turbojet for an influence, along flying trajectory calculating mass of import air and injection water, establishing the spray physics model/ mathematics model. Based on the equation of three-dimension Renault average N-S, air to adopt the limited physical volume method, the liquid adopts to the Lagrangian orbit model, with straight injecting the current method ,Simulate the field of air/ liquid two phase flow field to distribute of inlet to carry on mutually. The results indicate that spray have an effect on flow field configuration. It is concluded that the numerical model and CFD method for modeling spray flow processes in gas/liquid injector will be satisfactory. Similar to the SteamJet the United States puts forward, adopting water injection can satisfy to import the conditional request for pressure and temperature of compressor.