Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism

Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

하악 대구치 Ⅱ형 근심 근관에서 치근단 부위의 만곡도 조사

윤혜림,이동균,황호길 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: The purpose of this study was to evaluate the buccolingual curvature at the apical one third in type Ⅱ mesial canals of mandibular molars using the radius and angle of curvature. Materials and Methods: Total 100 mandibular molars were selected. Following an endodonticaccess in the teeth, their distal roots were removed. #15 H- or K-files (Dentsply Maillefer) were inserted into the mesiobuccal and mesiolingual canals of the teeth. Radiographs of the teeth were taken for the proximal view. Among them, type Ⅱ canals were selected and divided into two subgroups, Ⅱa and Ⅱb. In type Ⅱa, two separate canals merged into one canal before reaching the apex and in type Ⅱb, two separate canals merged into one canal within the apical foramen. The radius and angle of curvature of specimens were examined. Results: In type Ⅱ, mean radius of curvature in mesiolingual and mesiobuccal canals were 2.82 mm and 3.58 mm, respectively. The radius of the curvature of mesiolingual canals were significantly smaller than that of mesiobuccal canals in type Ⅱ, and especially in type Ⅱa. However, there were no statistically significant differences in radius of curvature between mesiobuccal and mesiolingual canals in type Ⅱb and there were no significant differences in angle of curvature between type Ⅱa and Ⅱb. Conclusion: In this study, type Ⅱ mesial canals of mandibular molars showed severd curvature in the proximal view. Especially, mesiolingual canals of type Ⅱa had more abrupt curvature than mesiobuccal canals at the apical one third.

동종 골수이식을 시행받은 환자에서 폐색성 세기관지염에 의하여 발생한 자발성 기종격동과 피하 기종

이병환,이제중,이연경,안재숙,김여경,황호인,박무림,조상희,정익주,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

저자들은 만성골수성백혈병으로 동종 골수이식과 이식편 부전으로 인하여 추가적인 말초혈액 조혈모세포이식을 시행 받은 환자에서 만성 이식편대숙주질환과 그 폐 합병증인 폐색성 세기관지염에 동반된 자발성 기종격동과 피하 기종이 병발한 1예를 경험하였기에 이를 보고하는 바이다. Obstructive lung disorders following after allogeneic bone marrow transplantation (BMT) in association with graft- versus-host disease (GVHD) contribute significant morbidity and mortality. We report a case of a 28-year-old man who developed spontaneous pneumomediatinum and subcutaneous emphysema complicating bronchiolitis obliterans after allogeneic BMT. He received an allogeneic BMT for chronic phase of chronic myeloid leukemia. Five months after BMT, he was boostered by allogeneic peripheral blood stem cells from the same donor due to graft failure. One month after the boostering, chronic GVHD developed and were treated with cyclosporine and steroid. The patients developed spontaneous pneumomediatinum and subcutaneous emphysema secondary to severe bronchiolitis obliterans 4 months after boostering donor cells. The air-leak syndromes were recovered by conservative management, including high-flow oxygen.

포도과피에 특이적으로 발현하는 stilbene synthase cDNA의 분리 및 과실발달 단계별 발현양상 분석

피재호,이호림 단국대학교 1999 論文集 Vol.34 No.-

Resveratrol (stilbene), a phytoalexin, is produced by stilbene synthase from malonyl-CoA and p-coumaroyl-CoA as substrates and its biosynthesis is induced by environmental stresses such as fungal elicitor and UV. In order to elucidate its role as an anti-stress compound, stilbene synthase (stsy) cDNAs were isolated from Jyoho grape skin, which has been known to contain high quantity of resveratrol, and their expressions were analyzed during the berry development. RT-PCR analysis showed that stilbene synthase gene was expressed in higher level in leaves and flowers than in berries, and its expression in berries was specific to skin. The expression was first detected 10 weeks postflowering in berry skin and increased as berries grew and ripened. Interestingly, another homolog was amplified from the RT-PCR analysis. Its expression was observed at the early developmental stage and reached at the maximum level 8 weeks postflowering, but was not detected thereafter. Both of the PCR produts were subcloned and they shared 70% homology in the nucleotide sequence. These results suggest that there are two or more stilbene synthase genes differentially regulated during the berry develepment.

거골 골절의 수술적 치료

최호림,이장근,최훈휘,최성우 대한골절학회 2003 대한골절학회지 Vol.16 No.1

목 적 : 거골 골절 수술적 치료 후 임상 결과를 분석하여 얻은 결과를 보고하며 향후 적절한 치료 방침을 결정하는데 도움이 되고자 한다. 대상 및 방법 : 1990년 3월부터 2000년 11월까지 본원에서 거골 골절로 치료한 환자 60례중 수술적 치료후 1년 이상 추시가 가능하였던 34례의 수술 전,후 및 추시 방사선 사진과 의무 기록지를 후향적으로 분석하였으며 현 환자의 상태는 외래 진료를 통한 직접 면담이나, 서면 또는 전화를 이용한 질문표에 대한 답변 등을 이용하여 분석하였다. 임상 결과의 판정은, 동통과 파행, 거골하 관절 및 족근 관절에서의 운동 범위등, 네 가지 요소에 따라 판정하는 Hawkins 평가 방법을 이용하였다. 결 과 : 총 34례중 25례(74%)에서 양호 이상의 만족스러운 임상 결과를 보였으나, 분쇄 정도가 심하여 조기에 정확한 해부학적 정복을 얻지 못했거나 주위 조직의 심한 손상 또는 타과적 손상이 동반되어 치료가 지연된 경우에서는 만족스럽지 못하였다. 무혈성 괴사는 Hawkins 징후가 보이지 않았던 8레중 6례에서 발생하였으며, 보존적 치료를 시행하여 만족할만한 임상 결과를 얻을 수 있었다. 결론 : 거골 경부 골절 치료후 임상 결과는 가능한 조기의 관혈적 정복을 통한 정확한 해부학적 정복 및 내고정이 무엇보다 중요하다고 생각되며, 더불어 적극적인 재활치료의 여부도 예우에 큰 영향을 줄 수 있다. 수술적 도달법에 의한 차이나 사용된 내고정물에 의한 임상 결과의 차이는 보이지 않았다. 무혈성 괴사 유무와 임상 결과는 반드시 일치하는 것은 아니었다. Purpose : To evaluate the clinical results and develop guidelines for surgical treatment of talus fracture. Materials and Methods : Among the 60 cases that were treated during March 1990 to November 2000, 34 cases were treated operatively and followed up for more than one year( range : 1~4.4 years). They were analyzed retrospectively with questionnaire directly or by telephone interview, radiograms and medical records. Clinical results were evaluated by Hawkins' scoring system. Results : 25 out of 34 cases showed satisfactory results. Unisatisfactory results were seen in cases that we couldn't achieve anatomical reduction due to severe communication, and also in case of delayed treatment due to severe communition, and also in case of delayed treatment due to associated trauma and soft tissue injury. Six out of 8 cases that showed no Hawkins' sign developed avascular necrosis. However, satisfactory results were achieved through conservative treatment. Conclusion : Satisfactory results could be achieved through early anatomical reduction and rigid internal fixation followed by aggressive rehabilitation. There was no differences in clinical results either by the surgical approach or method of internal fixation. Avascular necrosis was not essentially related to the clinical results.

전신성홍반성루푸스 환자에서 발생한 순수어농

김윤정,김해림,박시내,윤종현,이상헌,박성환,김호연 대한내과학회 2004 대한내과학회지 Vol.66 No.4

순수어농이란 발성언어에 대한 이해와 반복에 장애를 보이지만 읽기, 쓰기, 비발성언어에 대한 이해는 보존되는 경우를 일컫는다. 저자들은 45세 루푸스 환자에서 루푸스 혈관염으로 인해 일차 청각 피질을 포함한 왼쪽 관자이랑에 뇌경색이 발생하여 초래한 순수어농 1예를 경험하였기에 보고하는 바이다. Pure word deafness is characterized by an impairment of auditory comprehension and repetition of speech while a preservation of reading, writing, spontaneous speech and ability to comprehend non-verbal sounds. It is caused by trauma, infection in the temporal lobe of brain. In most cases, the lesions are bilateral, in the middle third of superior temporal gyri, but in a few cases unilateral lesions of dominant temporal lobe are localized. We experienced a case of 45 year-old right handed wonman with systemic lupus erythemato년 (SLE) who presented pure word deafness after left temporal lobe infarction including primary auditory center. Her comprehension was impaired, while reading and visual comprehension were normal. Audiometric tests and auditory evoked potentials disclosed no hearing defect. Brain MRI showed a left temporal lobe infarction without abnormal vascular finding in MRA. She wasdiagnosed as pure word deafness due to SLE vasculitis and managed with high dose steroid and cyclophosphamide therapies.