Interleukin-2와 결핵균 30 kDa 항원이 구개편도 및 말초혈액 T 세포 증식에 미치는 상승효과

박정규,박찬권,조은경,김화중,백태현,고필준,김병국,남부현,나기상,박찬일 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

Widespread use of BCG has not controlled tuberculosis, and more effective vaccines are clearly needed. Although chemotherapy will remain the mainstay of antituberculosis treatment, the use of adjunctive immunotherapeutic modalitites is attractive, particularly in persons with drug-resistant tuberculosis. Administration of IL-2 or IFN-γto tuberculosis patients enhance bacillary elimination. Cell-mediated immunity is the critical protective immune response in tuberculosis. Mycobacterial antigens are recognized by T cells and that elicit production of protective cytokines are potentially important vaccine antigens. The 30 kDa antigen is secreted in large quantities by growing mycobacteria. That antigen elicits greater proliferation in lymphocytes from healthy tuberculin reactors than healthy tuberculin nonreactors. In this study, the T lymphocyte proliferative responses to 30 kDa antigen from Mycobactrium tuberculosis H37Rv were examined by using tonsilar and peripheral blood lymphocytes from PPD(+) and PPD(-) tonsilectomized persons. When cultured with 30 kD antigen, tonsilar mononuclear leukocytes and T cells of PPD(+) demonstrated more ^3H-thymidine incorporation than PPD(-) persons (stimulation index was 2.5 and 1.9, 0.8 and 1.0, repectively). Peripheral blood mononuclear cells (PBMC) and peripheral blood T lymphocytes were shown the similar responses to this antigen. The combination of IL-2 and 30 kDa antigen elicited a significant proliferative responsiveness in tonsilar mononuclear leukocytes and T cells of PPD(+) persons (SI was 20 and 14.1). PBMC and peripheral blood T cells of PPD(+) persons were also shown a significant responsiveness, but PPD(-) persons did not show. These results demonstrate that the 30 kDa antigen and IL-2 have a synergistic stimulatory property in mycobacteria sensitizing lymphocytes.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

장기간의 복합트레이닝이 중년 남녀의 스포츠 의학적 변인에 미치는 영향

박상갑,권유찬,윤미숙,김은희 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The purpose of this study was to investigate the changes of sports medicine variables after 20weeks combined training. Subjects were consisted of 130 middle-aged males and females. The training program was separated treadmill running and muscular resistance training. The treadmill running was composed of 60-70% HRmax(Intensity), 30 minutes per day(Duration), 5 days per week(Frequency). The muscular resistance training was composed of 70% of 1RM(Intensity), 40 minutes per day(Duration), 5 days per week(Frequency). multiple ANOVA was used to evaluate change times. We found that weight, BMI %body fat, SBP and DBP were significantly decreased and LBM was significantly increased after 20 weeks combined training. In conclusion, combined training was shown to positive influence on body composition and blood pressure. Therefore, it is maybe though that 20 weeks combined training will benefit the prevention of life style related diseases.

Silver 처리한 Ceramic을 첨가한 PE film으로 포장된 김치의 저장성

박찬영,은종방,김종대 全南大學校 農業科學技術硏究所 1996 農業科學技術硏究 Vol.31 No.-

The quality changes of Kimchi packaged with LDPE(low density polyethlene, thickness 30㎛), AC30(added 3% ceramic and treated with silver in LDPE, thickness 30pm), AC60(added 3% ceramic and treated with silver in LDPE, thickness 60㎛) were studied during 10 days storage at 20℃. The concentration of carbon dioxide increased rapidly until 4 days of storage and decreased thereafter. Concentration of carbon dioxide was not different among packaging materials at the end of storage. Swelling in film bag of packaged Kimchi was not developed, not like being observed other packaging materials. Changes of pH, acidity and soluble solid contents in packaged kimchi were little differences among packaging materals during storage at 20℃. Number of lactic acid bacteria, content of vitamin C and sensory evaluation in packaged Kimchi were not significantly different among packaging materials. Unlike being expected, quality of Kimchi packaged with AC30, AC60 were not different from LDPE and shelf life of Kimchi was not extened. However, taste of Kimchi packaged in AC30 was better than LDPE by sensory evaluation after 4 days storage and swelling of its package was not shown. To extend shelf life of Kimchi using packaging materials we need to study for improvement of physical characteristics of the packaging materials and development of materials for active packaging

암 환자의 혈장 Transforming Growth Factor-β1 농도

박병규,하우송,이시은,이수진,박순태,박찬후,전지현,장정순 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

한국인의 대표적인 성인 고형 종양인 위암, 간암, 유방암과 소아 백혈병 및 2종의 소아 고형 종양 환자로부터 혈장 transforming growth factor-ß1 (TGF-ß1) 농도를 sandwich ELISA 분석법을 이용해 측정함으로써 TGF-ß1을 이 질환들에 대한 새로운 종양표지자 (tumor marker)로 사용할 수 있는지 검토하였다. 또한 연령 및 성별에 따른 혈장 TGF-ß1 농도의 정상치를 조사하였다. 신생아에서 70대까지 혈장 TGF-ß1 농도의 차이는 없었고 남녀간의 차이도 없었다. 위암 환자의 혈장TCF-ß1 농도는 16.0±6.8 ng/ml (평균 ±표준편차)로 정상 대조군의 TGF-ß1 농도 (8.3 ±5.0 ng/ml) 보다 유의하게 높았으나 간암, 유방암 환자의 혈장 TGF-ß1 농도는 대조군과 차이가 없었다. 그리고 위암 환자 16명, 간암 환자 8명, 유방암 환자 7명 중 각각 7명 (43.7%), 1명 (12.5%), 1명 (14.3%)에서만 혈장 TGF-ß1 농도가 증가되었다. 5명의 소아 백혈병 환자에서는 관해 (remission) 여부와 상관없이 혈장 TGF-ßl 농도가 모두 정상 범위에 있었으나 2명의 소아 고형암 환자에서는 종양 절제 전에는 혈장TGF-ß1 농도가 높았다가 절제 후 정상으로 떨어졌다. 결론적으로 1)정상인의 혈장 TGF-ßl 농도는 연령 및 성별에 따른 차이가 없다는 것을 알 수 있었고, 2)성인 고형암인 위암, 간암, 유방암에서는 낮은 민감도로 인해 TGF-ß1을 진단을 위한 선별 검사로 이용하기에는 부적절한 것으로 판단되었으며, 3) 정상 대조군보다 혈장 TGF-ß1 농도가 높았던 위암 환자와 종양 절제 전후로 혈장 TGF-ß1 농도가 민감하게 변했던 소아 고형 암 환자에 대해서는 향후 표본 수를 늘려 부가적인 연구를 해 야 할 것으로 사료된다. To evaluate the usefulness of transforming growth factor-ß1 (TGF-ß1)as a new tumor marker, we determined the plasma TGF-ß1 levels using sandwich ELISA assay in cancer patients. Patients with three most common adult cancers in Korea (stomach, liver and breast cancer) and children's cancers (leukemia and two kinds of solid tumor) were enrolled for the study. Furthermore, 39 individuals were subjected to age and sex-stratified plasma TGF-ß1 analysis. No statistical difference was demonstrated with respect to age or sex. The mean plasma TGF-ß1 level (16.0 ng/ml) of stomach cancer patients was significantly higher than that (8.3ng/ml) of controls. However, there was no difference among the mean plasma TGF-ß1 levels of liver, breast cancer patients and controls. Seven of 16 patients (43.7%) with stomach cancer, one of 8 (12.5%) with liver cancer, and one of 7 (14.3%) with breast cancer showed higher TGF-ß1 levels compared to controls. Plasma TGF-ß1 concentrations of five leukemic children remained in the normal range regardless of the remission state. In contrast, initial high TGF-ß1 levels from two children with solid tumors returned to normal range on surgical resection of tumors. From the above results, we could conclude that plasma TGF-ß1 levels of apparently healthy individuals seem to be rather constant irrespective of difference in age or sex, and the plasma TGF-ß1 has the limited value as a screening test for the diagnosis of aforementioned adult cancers because of its low sensitivity. Finally, additional studies need to be pursed for the large number of stomach cancer and pediatric solid tumor patients in order to reach a secure conclusion on the usefulness of plasma TGF-ß1 as a tumor marker in these patients.

개 원발성 면역매개성 혈소판 감소증 증례

유찬,임수정,이선희,박형진,김태신,송은식,김덕환,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 13-year-old, castrated male, Maltese-dog was admitted to the Veterinary Medical Teaching Hospital of Chungnam National University with the history fo petechiae. On physical examination, she had petechiae and ecchymoses at her back, bilateral inguinal region, and inner part of ear pinna. CBC indicated neutrophilia and severe thrombocytopenia. A few platelets, no autoagglutination of RBC, and no spherocyte were observed. Coagulation profile demonstrated normal PT, and aPTT. No significant findings was not found on serum biochemical profile and electolytes test. FNA of a splenic mass which was found on the abdominal radiograph and sonograph demonstrated extramedullary hematopoiesis, Proteinuria was observed on urinarysis. Primary immune-mediated thrombocytopenia was diagnosed by ruling out of other underlying disease, severe thrombocytopenia, and response to immunosuppressive therapy. Prednisone and antibiotics were administered orally and parenterally, respectively. After altering prednisone to azathioprine, the number of platelets was recovered to normal range and petechiae and ecchymoses were improved.

해동 대구살의 저온저장중 생균수와 맛의 변화 및 Microflora의 변화

박찬성,주은영 慶山大學校 1997 論文集 Vol.15 No.1

Frozen cod fillet block(7.0 x 4.6 x 0.6cm, 18 0.5g) was purchased from a supermarket in Taegu area. Three experimental groups of cod fillet, frozen-thawed cod fillet refrigerated at 5℃(R). frozen at -20℃(F) and repeated freezing and refrigerating(RFR) every other day, were incubated at each temperature, Changes in the aerobic plate counts(APC) and coliform bacteria of cod muscle during cold storage were investigated. Microbiological and sensory evaluations of frozen-thawed cod fillets were studied at each temperature. The APC and coliforms of frozen-thawed cod fillet immediately before cold storage were 6.5xl0⁴, l.gxlO²CFU/g of muscle, respectively. The APC of cod muscle R and RFR exceeded 10의7승 CFU/g within 8 days and 16 days. respectively, while that of cod muscle F were not exdeeded 10의7승 cells/g through the storage period. The coliforms of cod fillet R reached at 3.9xl0의6승 CFU/g of muscle after 16 days of refrigerated storage. The coliforms of cod fillet RFR reached at 3.7X10의6승 CFU/g of muscle after 24 days of storage while coliforms of F were less than 10³CFU/g of muscle through 46 days of frozen storage. The sensory shelf-life of cod fillets decreased linearly as APC increased. Cod fillets taste of R and RFR were unacceptable after 8 days and 20 days of storage, respectively, while frozen cod muscle (F)was acceptable 46 days of storage period. Among 30 islates of colifoms from cod muscle. 67% were isolated from R, 20% were from RFR and 13% were from F. Major isolates of coliforms were Klebsiella aerogenes(70%) and Citrobacter freundii(10%) while Escherichia coli was not islated. 20% of isolated conforms were unidentified.

분만시 지속적 또는 간헐적 투여에 의한 경막외 제통의 비교

박욱,이은찬,배상철,강규식,곽수달 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Background : Continuous epidural infusion of the mixed solution of local anesthetic (bupivacaine) and lipid-soluble opioid (fentanyl) has known as a more useful method than intermittent epidural injection for relief of pain during labor. The purpose of this study was to compare a clinical usefulness between continuous infusion and intermittent injection with epidural analgesia during labor. Methods: One hundred healthy parturients were divided into two groups as follows: Continuous epidural group (C-group); 50 parturients received epidural analgesia continuously with 0.167% bupivacaine and 0.000167% fentanyl in the speed of 10 ml h-1 at cervical dilatation (3-5 cm) and then injected 0.25% bupivacaine 5 ml additionally at complaints of severe labor pain. Intermittent epidural group (I-group); 50 parturients received inermittently 0.167% bupivacaine and 0.00083% fentanyl in the initial dose of 12 ml at cervical dilatation (3-5 cm) and then additionally used the same dose at severe labor pain. Result: There were no statistically significant differences in the clinical states of maternoneonate, pain free score, degree of motor block, mode of delivery, cause of Cesarean section, neonatal Apgar score and incidence of side effects between groups(Table 1.2.3.7.8). The total dose of bupivacaine and fentanyl, the duration of lst and 2nd delivery stages and the frequency of top up injection for incomplete relief of pain during labor appeared significant differences in statistics between groups (Table 4.5.6). Conclusion: It was similar to the effectiveness of maternal epidural analgesia during labor between groups by means of continuous or intermittent injection.

근저항의 복합트레이닝이 비만중년여성의 복부 지방과 HOMA index 및 혈청지질에 미치는 영향

박상갑,권유찬,김은희 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to investigate the changes of abdominal fat, insulin res[stance(HOMA index) and serum lipids after 12 weeks muscular resistance combined training. Subjects were composed of Fourteen middle aged obesity women. They have at least above 0.4 visceral fat and subcutaneous let ratio, The training program was separated aerobic training and muscular resistance training. The aerobic training was 70%HRR(Intensity), 60 minutes a day(duration), 3 days(mon, wed, fri) a week(Frequency), and the muscular resistance training was 70% of 1RM(Intensity), 60 minutes a day(duration), days(tue, thu) a week(Frequency) during 12 weeks. We found that VO₂max(0.28 ℓ/min) was increased after 12 WHIM combined training and %body fat(3.57%), total abdominal Fat(73.24cm³), visceral fat(18.94cm³), subcutaneous fat(54.29cm³), visceral fat and subcutaneous Fat ratio(0.02) were significantly decreased after 12 weeks combined training. Furthermore HOMA index and serum lipids were improved by muscular resistance combined training. In conclusion, muscular resistance combined training was shown to positive influence on body composition, insulin resistance and serum lipids. So it is maybe thought that muscular resistance combined training is very helpful for prevention of obesity.

위 MALT 림프종에서 다발성 림프절 전이로 오인된 동시성 림프절 결핵 1예

김성은,김규종,김도현,송준영,문대성,장리라,유찬희,문원,박무인,박선자,김영옥 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

28세 남자가 내시경적 점막절제술을 통해 저등급 위MALT 림프종으로 진단되었다. 병기설정을 위해 18F-FDG PET 검사를 시행하였고 우측 쇄골상부, 우측상/하부 기관주위, AP window 부위에 강한 섭취가 관찰되어 전이성 림프절로 판단하였다. 그러나, 우측 쇄골상부 림프절에서 시행한 절제생검에서 만성육아종성염증소견이 관찰되었고 조직의 결핵 중합효소연쇄반응에서도 양성 반응을 보였다. 2개월간 항결핵제의 사용 후 시행한 추적 컴퓨터단층촬영 검사에서 림프절 크기의 감소를 보여 최종적으로 위 MALT 림프종에서 광범위 림프절 전이로 오인된 동시성 림프절 결핵으로 진단되었다. A 28-year-old man was diagnosed as gastric MALT lymphoma in diagnostic EMR (endoscopic mucosal resection). He subsequently underwent an 18F-FDG PET. 18F-FDG PET showed intense multifocal uptake in right supraclavicular, right upper and lower paratracheal, and AP window areas that was considered radiologically to represent widespread metastatic lymphadenopathy. Excisional biopsy of a supraclavicular lymph node, however, revealed chronic granulomatous lymphadenitis with a positive reaction of Tb-nested PCR. After antituberculosis treatment for 2 months, a follow-up CT scan showed complete resolution of the lesions. Intense 18F-FDG uptake could be due to an infectious process such as synchronous tuberculous lymphadenitis. Therefore, this could mimic a malignant condition such as lymphoma with extensive lymph node metastasis due to a false-positive finding, which may lead to a misdiagnosis.