산소유리라디칼이 뇌조직 미크로좀분획의 Na⁺-K⁺-ATPase 활성도에 미치는 영향

오세문(Sae Moon Oh)손영숙(Young Sook Son),최길수(Kil Soo Choi),임정규(Jung Kyoo Lim),정명희(Myung Hee Chung) 대한약리학회 1982 대한약리학잡지 Vol.18 No.2

중추신경계의 혈관폐쇄 또는 충격손상에 의한 허혈병소에서 진행되는 병리적 변화에 산소유리라디칼이 중요한 역할을 할 것으로 시사되고 있다. 저자는 산소유리라디칼이 뇌조직에 미치는 영향 중 특히 신경세포 정지막전위 유지에 중요한 Na⁺-K⁺-ATPase의 활성도에 미치는 영향을 관찰하기 위하여 산틴산화효소 반응계와 뇌조직미크로좀을 이용하여 본 연구를 시행하였다. 미크로좀분획(microsomal fraction)을 산틴과 산틴산화효소와 함께 반응시켰을 때, 분획의 Na⁺-K⁺-ATPase의 활성도는 현저한 불활성화를 보인 반면, Mg⁺⁺-ATPase의 활성도는 별로 영향을 받지 않았다. 이 불활성화는 산틴과 신틴산화효소 중 어느 한 물질이라도 반응계에 존재하지 않는 경우에는 나타나지 않았고, 두 물질이 같이 반응계에 존재할 때 나타났다. 산틴과 산틴산화효소의 반응에서 생성되는 산소유리라디칼들 중, 어떤 것이 Na⁺-K⁺-ATPase불활성화에 관계하고 있는가를 알아보기 위하여, 산수유리라디칼 각각에 대하여 제독작용을 가진 효소나 화학물질을 사용하여 불활성화의 저해유무를 관찰하였다. O^-₂⋅의 제독효소인 superoxide dismutase, H₂O₂의 제독효소인 catalase와 ¹O₂의 제독물질인 1,4-diazabixyclo(2,2,2)octane을 각각 사용하였을 때, 이들 물질들이 농도에 비례하여 Na⁺-K⁺-ATPase의 불활성화가 저해되었다. 그러나 OH⋅의 제독물질인 mannitol은 뚜렷한 효과를 보이지 못했다. 이상의 결과는 산소유리라디칼들 중 O^-₂⋅, H₂O₂ 및 ¹O₂가 Na⁺-K⁺-ATPase의 불활성화에 관계하고 있고, OH⋅는 거의 관계하지 않는다는 것을 시사하여 주었다. 이로 미루어, 산소유리라디칼에 의한 뇌조직 Na⁺-K⁺-ATPase의 불활성화는 뇌 허혈병소에서 관찰되는 신경세로의 기능적 장해를 유발시키는 한 요인으로 사료되었다. The effects of xanthine-xanthine oxidase reaction on brain microsomal Na⁺-K⁺-ATPase activity were studied to see possible involvement of oxygen free radicals in pathologic change occurring in ischemic state of CNS accompanied by cerebral vascular occlusion or impact injury. When microsomal fraction was incubated with xanthine ana xanthine oxidase, Na⁺-K⁺-ATPase activity of the fraction was markedly inactivated (80% inactivation) whereas btssl Mg⁺⁺-ATPase was much less sensitive (less than 10% inactivation) compared to that of Na⁺-K⁺-ATPase. The inactivation was observed only in the presence of both xanthine and xanthine oxidase, not either of them alone, and the extent of inactivation was dependent on the concentration of xanthine. In an attempt to determine which of the oxygen species was responsible for the inactivation, the ability of various scavengers to overcome the inactivation was tested. Superoxide dismutase, catalase and 1,4-diazabicyclo(2,2,2)octane were shown to reverse the inactivation of the ATPase in dose-dependent manner. In contrast, mannitol as well as other OH⋅quenchers were ineffective in limiting oxygen radical-induced inactivation. Thus OO^-₂⋅, H₂O₂ and ¹O₂ were implicated to be mediators involved in the inactivation. Since oxygen radicals are suspected as being a cause of the peroxidative damaging process in train ischemia, the ATPase inactivation by oxygen radicals may be a possible contributing factor which gives rise to functional derangement of nerve cells observed in the pathologic process.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

산채류의보존과 일본식 절임

오영애,하귀현,박인경,김미향,김미정,김미경,정자림,이명숙,김순동 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

This paper showed the summarization of the preservative method of wild vegetables in the house and the precessing method of Japanese pickles. The preservative methods of wild vegetables were explained devided into long, short term, fresh state, heat treatment and drying. Salt, soybean fermented sauce, soybean fermented paste, lees of refined rice wine and residue in the preparation of bean curd were used as the materials of Japanese pickles.

부분육 처리공정에서 분리한 병원성 미생물의 성장특성

오영숙,임용숙,이신호 경산대학교 생명자원개발연구소 2001 생명자원과 산업 Vol.5 No.-

Growth characteristics of various pathogens isolated from cut meat processing plant were investigated to develop decontamination method during cut meat processing. Listeria monocytogenes, Stapylococcus aureus, Salmonella spp, Proteus vulgari, and Campylobacter coli, Escherichia coli were isolated from knife, cutting board, glove and conveyor belt and surface of carcass. Optimum growth temperature and pH of L. monocytogenes, E. coli and Salmonella spp which were major pathogens in meat processing environments were also investigated. Amaranthus lividus, Agarum cribrosum, Taraxacum platycarpum, roasted tea and Oolong tea except steam tea showed antimicrobial activity against isolated Escherichia coli, Listeria monocytogenes, and Salmonella spp.

일종합병원 입원환자의 불안에 관한 연구

오경옥,강문희,박영희,심희숙 충남대학교 간호과학연구소 2003 충남대 간호학술지 Vol.6 No.1

The purpose of this study was to evaluate the anxiety of inpatients in a general hospital in Daejeon. Data were collected through self-reported questionaires from January, 2003 to February, 2003, The anxiety was measured by Zung's Self-Rating Anxiety Scale. Analysis of data was done was descriptive statistics, t-test ANOVA and factor analysis. The results of the study were as follows : (1) The average score for the level of anxiety was 37.75±8.44 point (2) The anxiety items, such as sweating. apprehension, dyspnea, insomnia and restlessness showed the high score in inpatient. (3) Anxiety showed significant difference by education level(p<0.01), duration of hospitasization (p<0.01), prognosis(p(0.05), ward(medical/surgical) (p{0.05), experienc of admission (p<0.05). (4) According to the factor analysis, four factors of sweating, apprehension, dyspnea and restlessness showed high score in inpatient. Inpatient's anxiety characteristically showed more somatic symptoms than affective symptoms.

심한 낙타혹(Hump)의 형성을 동반한 제 2 형 막증식성 사구체신염(Dense Deposit Disease) 1예

오희정,유민아,이영숙,김미연,김승정,강덕희,윤견일,최규복,성순희 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.1

Dense deposit disease (DDD), known as type Ⅱ MPGN, is an uncommon form of glomerulonephritis. It is an acquired primary glomerular disease, characterised by electron microscopic evidence of a continous, dense membrane deposition replacing the lamina densa. There is associated alternative pathway complement activation and a C3 nephritic factor maybe present. Patients with dense deposit disease tend to be young at clincal onset and more commonly have persistent nephrotic syndrome, gross hematuria, and acute nephritis episode and persistent depression of the serum complement level. The prognosis of dense deposit disease is worse than that of typeⅠMPGN. We experienced a patient with dense deposit disease that presenting persistent nephrotic syndrome, gross hematuria and having subepithelial hump. It is a first report of dense deposit disease having subepithelial hump in Korea, therefore we report this case with the review of relevant literatures.

결핵 환자의 치료 순응도 향상을 위한 자기 간호교육의 효과

오영주,전진호,손혜숙,이종태 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Objectives : Good compliance to standard guideline might be the most important factor for success of tuberculosis treatment. This study was performed to propose the way to promote the compliance through evaluating the effect of self-care education in pulmonary tuberculosis treatment. Methods : The subjects were 100 patients(male 82, female 18) admitted in the national tuberculosis hospital, and study period was from December 1, 2000 to March 31, 2001. Study method was a direct interview survey with questionnaire that consisted of knowledges, attitudes, compliances about tuberculosis treatment, and some characteristics of subjects. The pre- and post-educational data were compared by chi-square test and paired t-test using SPSS(ver 10.0) with α-error=0.05. Results : The mean age of the subjects was 41.8±12.8years, and most of them was male. Half of them was still drinking and smoking, and the tuberculosis treatment categories of WHO was categoryⅢ 42%, categoryⅣ 22%, categoryⅡ 21%, categoryⅠ 15% when admitted. Average prevalent period was 56.8 months, and 68% of them showed positive sputum smear test. The average knowledge level was increased from 6.88±1.85 when admitted, and 8.02±1.57 when two months after(p〈0.001), that means, the effect of education was recognized. In contrast, recognition and attitudes showed no difference. And, the frequency of subjective aggravation was higher in the group of low compliance: voluntary medication stop(p=0.022). Conclusions : This study had some limitations; lack of representativeness because the subjects were limited to the patients in one tuberculosis hospital, and, not sufficient sample size etc. Nevertheless, because the effect of the education was partly recognized, to enhance the effect of tuberculosis treatment, the contents of self-care education would be changed toward the practice with a close monitoring of the patients' compliance.

우리나라 생명공학 연구 개발의 현황과 육성방향

오을임,박민희,이숙영 朝鮮大學校 社會科學硏究所 2000 社會科學硏究 Vol.21 No.2

면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

대학 구내식당의 음식쓰레기 감량을 위한 교직원의 의식 및 이용실태 조사

한재숙,오옥희,최영희 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.1

The goal of this study was to find the way of reduction on food waste in the university cafeteria. A survey was conducted on the 500 faculty members using Yeungnam University cafeteria, and if illustrates the faculty's use and perception for the reduction of food waste. Its results can be summarized as follows 32.0% of the total users use the university cafeteria everyday, also 86.6% of the users preferred Korean style dish. Female users left more food than the male users resulting in more food wastes. The male users resulting in more food waste and the most leaving food was Kimchi than the other types of foods. The most favorite stew and side dish turned out to be Yukgaejang and Bulgogi respectively, also Kimchibokkumbab was the preferred dish. But they disliked Sunjiguk, Ginger, Jellyfish and Mackerel pike. The perception of male users on the reduction of food waste was lower than that of female users. The best way of food waste treatment recognized by the users were utilizing the waste food as fodder for animals or fertilizer.