Improving Upper Limb Spasticity in Patients with Stroke by Electroacupuncture Therapy: a Pre- and Post-Treatment Study

Lim Sung Min,Go Eunji,Lee Jungsup,Lee Go Eun,Kim Eun Joo,Son Chihyoung 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.6

Background: Post-stroke upper limb spasticity (PS-ULS) causes a decline in the quality of life of patients by reducing their ability to perform normal daily activities. Objectives: We performed pre- and post-treatment analyses of the changes in stroke patients with ULS following constant electroacupuncture therapy (EAT). Methods: Thirty patients with PS-ULS underwent 12 sessions of EAT over 4 weeks. After performing acupuncture therapy at four acupoints on the affected arm (LI11, LI10, LI4, and TE5), electrostimulation (60 Hz) was performed for 20 min. The modified Ashworth scale (MAS) and the Fugl–Meyer assessment of the upper extremity (FMA-UE) were measured as the outcome variables. Results: Following EAT, there was a significant decrease in the elbow MAS score (p < 0.001), a significant decrease in the wrist MAS score (p < 0.01), and a significant increase in the FMA-UE score (p < 0.001). Adverse events related to EAT were not reported. Conclusion: EAT decreased upper limb spasticity and improved functional recovery at the elbow and wrist. Large-scale and rigorous clinical trials are needed to verify the efficacy of EAT.

Effect of Action Observation Training with Auditory Feedback for Gait Function of Stroke Patients with Hemiparesis

( Hyeong Min Kim ),( Sung Min Son ) 대한물리치료학회 2017 대한물리치료학회지 Vol.29 No.5

Purpose: Previous studies have reported that action observation training has beneficial effects on enhancing the motor task, such as bal-ance and gait functions. On the other hand, there have been few studies combined with action observation training and auditory feedback. The purpose of this study was to determine the effects of action observation training with auditory feed-back on the gait function in stroke patients with hemiparesis Methods: A total of 24 inpatients with post-stroke hemiparesis were assigned randomly to either an experimental group 1 (EG 1, n=8), experiment group 2 (EG 2, n=8), control group (CG, n=8, EG 1). The EG 2 and CG watched video clip demonstrating three functional walking tasks with auditory feedback, without auditory feedback, and showing a landscape image, respectively. The exercise program consisted of 30 minutes, five times a week, for four weeks. The participants were measured to 10MWT (10 m walk test), 6MWT (6 min-utes walking distance test), TUG (timed up and go test), DGI (dynamic gait index), time and steps of F8WT (figure-of-8 walk test). Results: In the intra-group comparison after the intervention, EG 1 and EG 2 showed a significantly different gait function (10MWT, 6MWT, DGI, TUG, F8WT) (p<0.05). In the inter-group comparison after intervention, EG 1 showed significant improvements in the entire gait parameters and EG 2 only showed significant improvement in DGI and TUG compared to CG (p<0.05). Conclusion: These findings show that action observation training with auditory feedback may be used beneficially for improving the gait function of stroke patients with hemiparesis.

배추의 형질전환용 선발항생제로서 Paromomycin의 이용

조미애,민성란,고석민,유장렬,이준행,최필선,Cho, Mi-Ae,Min, Sung-Ran,Ko, Suck-Min,Liu, Jang-Ryol,Lee, Jun-Haeng,Choi, Pil-Son 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.4

정상' 배추와 '서울' 배추의 배축절편을 선발마커로서 hygromycin 저항성유전자를 갖고 있는 pCAMBIA1301와 paromomycin저항성유전자를 갖고 있는 pPTN290으로 각각 형질 전환된 LBA4404 또는 EHA101균주와 공동배양한 후 선발배지에서 배양하면서 형질전환체를 선발하였다. 형질전환빈도는 사용된 항생제와 품종에 따라서 현저하게 차이가 있었으며, 특히 paromomycin은 hygromycin보다 효과적이었고 정상 배추는 서울배추보다 양호하였다. 가장 높은 형질전환빈도는 (0.70%) 100mg/L paromomycin이 첨가된 선발배지에서 정상배추의배축을 배양할 경우 얻어졌다. GUS양성반응으로 확인 한 결과 정상배추에서 9개체와 서울배추에서 3개체를 각각 얻었으며, 온실에서 생장한 후 $T_1$종자를 수확하였다. $T_1$ 종자를 다시 발아시켜 유식물체를 얻은 후 GUS양성반응을 확인함으로서 외래유전자가 안정적으로 발현하고 있음을 확인하였다. Hypocotyl explants of Chinese cabbage (us. 'Jeong Sang' and 'Seoul') produced adventitious shoots on Murashige and Skoog (MS) basal medium supplemented with 4mg/L $AgNO_3$, 5 mg/L acetosyringone, 4 mg/L 6-benzyladenine and 3mg/L alpha-naphthaleneacetic acid (SI) after cocoultivation with strains of Agrobacterium tumefaciens (LBA4404) harboring the pCAMBIA1301 and the $_PPTN290$ containing hygromycin-resistance gene and paromomycin-resistance gene as a selectable marker genes, respectively. There was a significant difference in the frequency of transgenic plants depending on antibiotics and cultivars used. Paromomycin was better than hygromycin, and cultivar 'Jeong-sang' was higher than 'c.v. Seoul' in the frequency of transgenic plants. In particular, the highest frequency (0.70%) of transgenic plants was obtained from selection medium (SI) containing 100mg/L paromomycin in c.v., 'Jeong-sang' GUS positive response were obtained 9 plants and 3 plants from the cultivars, 'Jeong-sang' and 'Seoul', respectively. They were grown to maturity in a greenhouse and normally produced $T_1$ seeds. GUS histochemical assay for progeny $(T_1)$ revealed that the transgenes were expressed in the plant genome.

Surgical Outcomes of Adrenocortical Carcinoma; 20 Years of Experience in a Single Institution

Min Jhi Kim,Eun Jeong Ban,Soo Jung Jung,Hai Young Son1,Cho Rok Lee,Sang-Wook Kang,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Cheong Soo Park 대한갑상선-내분비외과학회 2014 The Koreran journal of Endocrine Surgery Vol.14 No.4

Purpose: Adrenocortical carcinoma (ACC) is a rare malignant tumor. Early detection is difficult and prognosis is poor. We report on 20 years of ACC surgical experience at our institution. Methods: This study included 32 ACC patients who underwent surgical resection at the Department of Surgery of the Yonsei University Health System in South Korea between January 1990 and February 2012. We reviewed these 32 patients and retrospectively analyzed long-term clinical outcomes and prognosis after radical surgery for ACC. Results: The median age of the 32 patients at diagnosis was 42.25 years (range 3∼81 years). There were 16 (50%) female and 16 (50%) male patients. Mean tumor size was 12.36 cm (range 1.8∼20 cm). Twenty-five patients (78.12%) had nonfunctioning tumors while the other seven patients (21.87%) had functioning tumors. Seventeen patients (53.12%) were classified as stage II, two (6.25%) as stage III, and 13 (40.62%) as stage IV. Fourteen patients underwent radical surgical resection, while 14 patients received adjuvant chemotherapy, two received adjuvant radiotherapy, and two received adjuvant chemoradiation. Four patients were lost to follow-up. Among the remaining 28 patients, 15 patients survived. The 5- and 10-year overall survival was 60.6% and 37.8%, respectively (median survival=85±24.3 months). Seventeen patients (53%) experienced disease recurrence. Five- and 10-year recurrence-free survival was 41.5% and 29.7%, respectively (median survival=18±5.5 months). Conclusion: Early stage at diagnosis and surgical resection were the most important prognostic factors associated with prolonged survival. The role of additional therapy remains controversial and new agents should continually be evaluated for efficacy.

함입된 상악 중절치의 외과적 재위치 후 예후

민성진,최형준,류정아,김성오,이제호,최병재,손흥규 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3

한국과 일본 사무작업자에 대한 Type A 行動特性과 스트레스

孫銀一,鄭玟相 진주여자전문대학 1997 論文集 Vol.20 No.-

A Questionnaire was administered to the 1674 clerk workers who work in motor company in Korea and Japan. The questionnaire included the sociodemographic status working conditions. life habits, Type A behavior question, and stress and arousal check list. In general Koreans have marks in Type A behavior, and tend to have many marks in production department. However, Japanese tend to have many marks in purchasing department. Korean had lower scores in stress than Japanese. The important stressors were hope and goal in the future work environment in Korean, but in Japanese, were home life and job satisfaction.

한국산 관박쥐 및 긴날개박쥐에 잔류하는 유기염소화합물

민병윤,손성원,이호원 경남대학교 환경문제연구소 1987 환경연구 Vol.9 No.-

1985년 9월부터 1986년 7월까지 경상남도 마산시와 통영군에서 관박쥐 및 긴날개박쥐를 채집하여 박쥐 체내에 잔류하는 유기염소화합물 (PCB, BHC, DDT)의 농도를 조직 (간, 흉근, 잔해)별로 측정한 결과 전 개체로부터 이들 화합물이 높은 농도로 검출되었고, 이로부터 이들 화합물의 강한 잔류성이 시사되었다. 또한 검출된 화합물의 농도는 박쥐의 종류 [관박쥐 (평균), PCB(0.25 ppm) > BHC(0.19 ppm) > DDT(0.05 ppm) ; 긴날개박쥐 (평균), DDT(1.97 ppm) > BHC(1.63 ppm) > PCB(0.30 ppm) > 및 조직부위 [관박쥐, 잔해 (0.70 ppm) > 간 (0.40 ppm) > 흉근(0.37 ppm) ; 긴날개박쥐, 잔해 (5.61 ppm) > 흉근(4.96 ppm) > 간 (1.12 ppm)]에 따라 달랐으며 이는 이들 화합물외 사용 형태, 물리화학적성질 및 박쥐류의 서식환경, 식성과 생리등에 의해서 결정됨이 시사되었다. Residue levels of PCBs, BHC isomers and DDT compounds were examined in two species of bats, the greater horseshoe bat, Rhinolophus ferrumequinum korai and the long-fingered bat, Miniopterus schreibersi fuliginosus, collected from Masan City and Tongyoung-gun in Korea during the period from September 1985 to July 1986. From the high residue levels of the organochlorine compounds in the two species, prolonged persistency of these compounds were suggested. Further, the difference in contaminant levels in the two species (R.f.korai, PCB 0.25ppm > BHC 0.19ppm > DDT 0.05ppm; M. s. fuliginosus, DDT 1.97ppm > BHC 1.63 ppm > PCB 0.30 ppm) and in the tissues examined (R.f. korai, carcass (). 70ppm > liver 0.40ppm > breast muscle 0.37ppm ; M. s. fuliginosus, carcass 5.61ppm > breast muscle 4.96ppm > liver 1.12ppm) seemed to be due to the disparity in uses and physio-chemical properties of the compounds, and in habitat, food and physiology between the two species.

성별에 따른 청소년의 스트레스와 자아개념의 관계

송광민,차영아,남상인 순천향대학교 인문과학연구소 2008 순천향 인문과학논총 Vol.21 No.-

This study aims to examine the relationship between the stress and the self-concept of the adolescents according to the gender. This study used the data of Korea Youth Panel Survey (KYPS) Performed by Korea Institute for Youth Development. Prior to the study, factor analysis was performed in order to examine how the dimension of the stress and self-concept could be structured. As a result of factor analysis, the stress could be divided into four dimensions of the stress from parent, study, friends and appearance/material, while the self-concept was divided into four dimension of natural self, self-torment self, deviated self and leading self. Among four factors of self concept, self-torment self was proved to have the closest relationship with stress. And there was no big difference in this result when compared between both genders. As a result of gender comparison for multi-variable regression analysis with self concept as dependent variable, the friend stress was the most influential factor in case of male students while appearance/material stress was the most influential factor in case of female students.

Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산

손홍주,민관필,이상준 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

하수처리장의 활성오니를 분리원으로 하여 수십종의 PHA 생산균을 분리하였다. 일반적으로 hydroxyvalerate monomer unit의 전구물질로 알려져 있지않은 glucose로부터 비교적 많은 PHA를 생산하는 균주를 공시균으로 선정하여 형태학적, 배양적, 생리학적 제 특성을 검토한 결과 Pseudomonas 속으로 동정되었다. 균체 생육을 위한 최적 배양온도 및 배양 pH는 각각 37℃와 7.0이었으며, 최적 탄소원으로서 glucose 1%, 최적 질소원으로서 (NH_4)_2SO_4 0.2%, K_2HPO_4 0.3%, KH_2PO_4 0.45%였다. 최적 PHA 생산조건을 조사하기 위하여 2단계 배양법을 이용하였다. PHA 생산은 배지성분중 NH_4, SO_4, Mg가 결핍되었을 때 향상되었고, 그중 NH_4의 결핍시 PHA 축적률과 HV monomer의 함량이 가장 높았다. C/N molar ratio 95.2에서 PHA 축적률이 가장 높았다. 공시균주 Pseucomonas sp. HJ는 alkane, alkanoic acid, alcohol을 탄소원으로 하여 PHB/HV를 생산하였다. PHA의 생산량과 HV monomer의 함량은 이용된 기질에 따라 다양하였으며, 특히 hexadecane와 propionate를 탄소원으로 하였을 때 PHA중의 HV monomer의 함량이 49∼74mol%로 매우 높았다. ^1H-NMR로서 공시균으로부터 분리정제된 PHA의 조성을 분석한 결과 PHB/HV copolymer임을 알 수 있었다. To produce PHA(polyhydroxyalkanoic acid) from microbe, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source(glucose) that was not generally considered as precusor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were 37℃ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH_4)_2SO_4 0.2%, as a nitrogen source, K_2HPO_4 0.3%, and KH_2PO_4 0.45%. To investigate the optimal condition for PHA production two-step cultivation method was employed. PHA production was induced by deficiency of NH^+_4, SO^2_4, Mg^+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH^+_4 stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to produc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by ^1H-NMR analysis.

Pichia ohmeri에 의한 악성외이도염 1예

손준성,신상엽,민태훈,장현하,정숙인,오원섭,김성민,백경란,송재훈 대한감염학회 2002 감염 Vol.34 No.5