척수를 절단한 흰쥐에 태생기의 Serotonin을 함유한 솔기핵을 이식한 후 척수의 형태학적 재생 및 기능에 관한 연구

김종중,정윤영,장인엽,정주현,문정석,김흥중 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.6

느타리버섯 신품종 육성 연구(I) : 병재배용 신품종『진미』느타리버섯의 특성

지정현,주영철,최종인 한국버섯학회 2004 한국버섯학회지 Vol.2 No.4

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 진미느타리버섯의 균사생장적온은 25∼30℃, 버섯발생 및 생육온도는 15±1℃이고 갓색은 회색이며 얕은깔대기형으로 발이수가 많았다. 배양일수는 20℃에서 22일, 초발이 소요일수는 4일이며 가늘고 긴형으로 농가실증시험에서 병당수량은 145.9g으로 높은 편이었다. 버섯의 갓색과 갓형태, 이형개체 발생,, 균사생장량, 종균배양기간 등에 대한 균일성도 양호하였고, 세균성 갈변병에 대한 저항성은 소정도로 나타났으나 푸른곰팡이병은 발생되지 않았다. 또한 균사 활력이 강하고 15 이하에서는 진한회색을 나타내며 육질이 부드럽고 대가 백색으로 깨끗한 직립형의 특성을 보유하고 있다. Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around 25∼30℃ and that for the pinheading and growth of fruitbody was around 15±1℃. Around 22 days at 20℃ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

치주병원균에 대한 유산균의 억제효과

정하나,김영준,정현주,오종석 전남대학교 치과대학 1999 전남치대논문집 Vol.11 No.1

This study was performed to evaluate the effect of hydrogen peroxide-producing Lactobacillus acidophilus V-20 on the replication of periodontal pathogens, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. When A. actinomycetemcomitans and P. gingivalis were incubated alone and in the combination with L. acidophilus V-20, the viable cell numbers of A. actinomycetemcomitans and P.gingivalis were compared between those cultures. The effect of S. mutans, E. durans, and L. lactis on the replication of A. actinomycetemcomitans and P. gingivalis was also evaluated. The change of periodontal indexes(probine depth, gingival index, GCF volume) and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides in subgingival plaque sample were evaluated following gargling of fermented milk made from L.acidophilus V-20 for 1 month on patients with periodontal disease in maintenance phase. In the mixed culture of L. acidophilus V-20 and A. actinomycetemcomitans or P. gingivalis, the replication of A. actinomycetemcomitans or P. gingivalis was completely inhibited. But in the mixed culture of P. gingivalis and hydrogen peroxide-nonproducing Lactobacillus casei, the viable cell numbers of P. gingivalis was not decreased when compared with the numbers in the mixed culture of P. gingivalis and L. acidophilus V-20. In the mixed culture of A. actinomycetemcomitans and S. mutans, E. durans, or L. lactis, the viable cell number of A. actinomycetemcomitans was not almost changed when compared with the numbers in the culture of A. actinomycetemcomitans alone. And in the mixed culture of P. gingivalis and E. durans or L. lactis, the viable cell numbers of P. gingivalis was not almost changed compared with the counts in the culture of P. gingivalis alone. But the replication of P. gingivalis was completely inhibited in the mixed culture of P. gingivalis and S. mutans. When the change of periodontal indexes following gargling of fermented milk was compared with baseline, probing depth and gingival index were not changed, but GCF volume was significantly decreased(p<0.05). And when the viable cell numbers of microorganisms in subgingival plaque sample were compared with baseline, total viable cell number was almost unchanged and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides were significantly decreased(p<0.05). These results suggest that L. acidophilus V-20 inhibit the replication of A. actinomycetemcomitans and black pigmented bacteroides by the formation of hydrogen peroxide.

유기농 자재의 시용이 토양의 이화학적 성질과 상추, 가지의 생육 및 체내성분에 미치는 영향

이종성,김기영,황필성,오주성,정원복,정순재 동아대학교 2001 大學院論文集 Vol.26 No.-

This study was carried out to investigate the effect of microbial fertilizers on the application of organic farming materials in soil on growth and yields of leaf lettuce, eggplant. Three microbial fertilizers, Aika, Kalen-gold and EMRO-EM were used. The results were summarized as follows ; 1. The effect of soil chemical properties after application organic arming materials, The amounts of pH, EC, P2O5 and O.M. treated all plot were few of change. 2. Change of soil microbial after application organic farming materials, The number of bacteria, actinomycetes and fungi in N, P, K treated plot showed the increased tendency, to the contrary, The number of bacteria, actimycetes in Aika, kalen-gold and EMRO-EM treated plot showed the increased, The number of fungi showed the dicreased tendency 3. The application effects of Aika, Kalen-gold and EMRO-EM on the growth, yield and components of leaf lettuce and eggplant in comparison with chemical fertilizers plot was not a difference.

토끼의 간뇌에서 NPY 분비세포에 대한 면역조직화학적 연구

정주현,문정석,김종중,장인엽,정윤영,김흥중 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The distributions of neuropeptide Y(NPY) in the diencephalon of the adult rabbit was investigated using indirect immunohistochemistry. The method showed that neuropeptide Y had a widespread distributions and was present in extremely high concentration in the hypothalamic nuclei of the rabbit. A few number of NPY-containing perikarya was found in the arcuate nucleus, paraventricular nucleus. medial to the fornix. Neuropeptide Y-immunoreactive axons and terminals in the hypothalamus were found in most hypothalamic nuclei. The highest concentrations of neuropeptide Y-containing fibers were found in the arcuate nucleus. paraventricular nucleus. and alao the supraoptic nucleus. dorsomedial hypothalamic nucleus. preoptic area showed relatively high concentration of neuropeptide Y-immunoreactive fibers. The extremely high concentations of neuropeptide Y in the bypothalamus indicate important role in many brain functions

農民所得增大를 위한 고구마의 栽培와 利用에 관한 硏究

黃鍾奎,梁熙天,崔定植,權涌周 全北大學校 1977 論文集 Vol.19 No.-

New varieties of sweet potato Sinmi and Hwangmi were cultivated in the reclaimed hilly area of four kinds of soil series in Jeonbug province and their growth conditions such as cutting time, row spacing, application of fertilizers and plant growth regulators and covering effect were investigated. Besides these studies on cultivation of sweet potatoes, experiments on the change of chemical components of sweet potato tubers, adaptibility for storage and drying of sliced sweet potato tubers were carried out. The results are summarized as follows : 1. The early cutting of May 20, alley of 90 X 20cm, application of potassium(28kg/10a)and covering produced the high yield of sweet potatoes. The apllication of OED green did not increase the yield of sweet potatoes, 2. The yield of Hwangmi was higher than that of Sinmi. These two varieties of sweet potatoes grew well in all the test soil series except Bansan. By early cutting of May 20, the highest yield of Hwangmi was obtained in Songjung series soil and the highest yield of Sinmi was in Yesan. 3. Starch content was higher in Sinmi than in Hwangmi while yield of starch per unit area was high in Hwangmi because of its high yield of sweet potato tubers. Both Hwangmi and Sinmi showed very high water content of more than 70 per cent. The highest yield of total solid matter from Sinmi was on September 15 and that from Hwangmi on September 30. Sinmi and Hwangmi harvested on September 30 showed the highest starch content. 4. Variation in water content, starch, soluble sugar, protein and fat content during storage was minimum in the sweet potatoes stored in the temperature controlled storage chamber and was maximum in those stored in the cave under ground. As a result, percent of grade A sweet potatoes remaining was highest in the temperature controlled storage chamber and lowest in the cave under ground. 5. Hwangmi was more susceptible to Rhizopus nigricans than Sinmmi, especially under the humid condition. 6. Shape and thickness of slice did not affect the yield of dried sweet potatoes but affected the time needed for drying. Depending on the thickness and the shape of slice, variations of 36 hours and 12 hours in drying time were observed respectively. Drying table was most effective in drying of sweet potatoes and drying rack was also effective and required 2 days less time than mat. Dipping in water did not show any effect on drying time but improved the color of dried sweet potatoes.

흡연에 의한 급성 호산구성 폐렴 2예

박종빈,김학렬,주현준,유태양,신성남,신정현,송정섭,황기은,김소영,양세훈,정은택 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

최근 흡연을 시작한 젊은 성언에서 급성 호산구성 폐렴의 발생에 대한 증례가 보고되고 있다. 급성 호산구성 폐렴은 대개 발열을 동반하기 때문에 초기에는 감염성 폐렴으로 잘못 진단, 치료되는 경우가 많다. 아직 정확한 병태 생리 및 조직학적 소견은 밝혀져 있지 않지만 병력 청취 및 임상 양상, 방사선 소견의 관찰을 통해 급성 호산구성 폐렴을 의심하고, 진단을 위해 기관지 폐포 세척액 검사를 시행한다면 좀 더 쉽게 진단 내릴 수 있을 것이다. 본 저자들은 최근 처음 시작한 흡연에 의해 발생한 급성 호산구성 폐렴을 진단하고, 스테로이드를 투여하여 성공적으로 치료한 환자 2예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Acute eosinophilic pneumonia(AEP) is characterized by acute febrile respiratory illness associated with diffuse pulmonary infiltration and pulmonary eosinophilia. The specific etiology for acute eosinophilic pneumonia is elusive. By some investigators, cigarette smoking is suggested as a causative substance which can cause AEP. In recent, the authors experienced two cases of AEP following cigarette smoking. Both cases had characteristic features including age around 20 years, new onset smoking before occurance of AEP, diffuse infiltration on chest radiography, pulmonary eosinophilia based on bronchoalveolar lavage and acute improvement after steroid therapy. These clinical features are resemble with previous smoking induced AEP case reports. Base on these clinical features, cigarette smoking associated AEP could be diagnosed more easily.

지혈제 오염이 콤포머의 전단결합강도에 미치는 영향

허정무,곽주석,이황,이수종,임미경 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

One of this latest concepts in bonding are "total etch", in which both enamel and dentin are etched with an acid to remove the smear layers, and "wet dentin" in which the dentin is not dry but left moist before application of the bonding primer. Ideally, the application of a bonding agent to tooth structure should be insensitive to minor contamination from oral fluids. Clinically, contaminations such as saliva, gingival fluid, blood and handpiece lubricant are often encountered by dentists during cavity preparation. The aim of this study was to evaluate the effect of contamination by hemostatic agents on shear bond strength of compomer restorations. One hundred and ten extracted human maxillary and mandibular molar teeth were collected. The teeth were removed soft tissue remnant and debris and stored in physiologic solution until they were used. Small flat area on dentin of the buccal surface were wet ground serially with 400, 800 and 1200 abrasive papers on automatic polishing machine. The teeth were randomly divided into 11 groups. Each group was conditioned as follows: Group 1: Dentin surface was not etched and not contaminated by hemostatic agents. Group 2: Dentin surface was not etched but was contaminated by Astringedent® (Ultradent product Inc., Utah, U.S.A.). Group 3: Dentin surface was not etched but was contaminated by Bosmin®(Jeil Pharm, Korea).). Group 4: Dentin surface was not etched but was contaminated by Epri-dent®(Epr Industries, NJ, U.S.A.). Group 5: Dentin surface was etched and not contaminated by hemostatic agents. Group 6: Dentin surface was etched had contaminated by Astringedent®. Group 7: Dentin surface was etched and contaminated by Bosmin®. Group 8: Dentin surface was etched and contaminated by Epri-dent®. Group 9: Dentin surface was contaminated by Astringedent®. The contaminated surface was rinsed by water and dried by compressed air. Group 10: Dentin surface was contaminated by Bosmin®. The contaminated surface was rinsed by water and dried by compressed air. Group 11: Dentin surface was contaminated by Epri-dent®. The contaminated surface was rinsed by water and dried by compressed air. After surface conditioning. F2000® was applicated on the conditoned dentin surface. The teeth thermocycled in distilled water at 5℃ and 55℃ for 1,000 cycles. The samples were placed on the binder with the bonded compomer-dentin interface paralled to the knife-edge shearing rod of the Universal Testing Machine(Zwick Z020, Zwick Co., Germany) running at a cross head speed of 1.0mm/min. Group 2 showed significant decrease in shear bond strength compared with group 1 and group 6 showed significant decrease in shear bond strength compared with group 5. There were no significant differences in shear bond strength between group 5 and group 9, 10 and 11.

군산 연안 해역에서의 부영양화 제어에 관한 연구

김종구,정태주 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Gunsan coastal area is one of region increasing pollution problems. To improve water quality, the reduction of these nutrients loads should be indispensible. In this study, the three-dimensional numerical hydrodynamic and ecosystem model were applied to analyze the processes affecting the eutrophication. In field survey, the average concentrations of dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus(DIP) at surface waters were found to be 0.43㎎/ℓ and 0.03㎎/ℓ respectively, which were exceeding second grade of water quality criteria. In hydrodynamic modelling, the comparison between the simulated and observed tidal ellipses showed fairly good agreement. The ecosystem model was calibrated with the observed data in study area. The simulated results of DIN were fairly good coincided with the observed values within relative error of 32.39%, correlation coefficient(r) of 0.99. In the case of DIP, the simulated results were fairly good coincided with the observed values within relative error of 24.26%, correlation coefficient(r) of 0.82. The simulations of DIN and DIP concentrations using ecosystem model were performed under the conditions of 20~80% reductions for pollutant loading. At simulation results, concentration of DIN and DIP were reduced to 20~80% and under 10% in case of the 80% reduction of polltuant loading, respectively.

광조사 방식이 복합레진의 중합과 누출에 미치는 영향

박종진,박정원,박성호,박주명,권태경,김성교 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

The aim of this study was to investigate the effect of light irradiation modes on polymerization shrinkage, degree of cure and microleakage of a composite resin. VIP^™ (Bisco Dental Products, Schaumburg, IL, USA) and Optilux 501^™(demetron/ Kerr, Danbury, CT, USA) were used for curing Filtek^™ Z-250(3M Dental Products, St. Paul., MN, USA) composite resin using following irradiation modes: VIP^™ (Bisco) 200mW/cm^2 (V2), 400mW/cm^2 (V4), 600mW/cm^2 (V6), Pulse-delay (200 mW/cm^2 3 seconds, 5 minutes wait, 600mW/cm^2 30seconds, VPD) and Optilux 501^™ (Demetron/Kerr) C-mode (OC), R-mode (OR). Linear polymerization shrinkage of the composite specimens were measured using Linometer (R&B, Daejeon, Korea) for 90 seconds for V2, V4, V6, OC, OR groups and for up to 363 seconds for VPD group (n=10, each). Degree of conversion was measured using FTIR spectrometer (IFS 120 HR, Bruker Karlsruhe, Germany) at the bottom surface of 2 mm thick composite specimens. V2, V4, V6, OC groups were measured separately at five irradiation times (5, 10, 20, 40, 60 seconds) and Or, VPD groups were measured in the above mentioned irradiation modes (n=5, each). Microhardness was measured using Digital microhardness tester (FM7, Future-Tech Co., Tokyo, Japan) at the top and bottom surfaces of 2mm thick composite specimens after exposure to the same irradiation modes as the test of degree of conversion(n=3, each). For the microleakage test, class V cavities were prepared on the distal surface of the ninety extracted human third molars. The cavities were restored with one of the following irradiation modes: V2/60 seconds. V4/40 seconds, V6/30 seconds, VPD, OC and OR. Microleakage was asessed by dye penetration along enamel and dentin marigns of cavities. Mean polymerization shrinkage, mean degree of conversion and mean microhardness values for all groups at each time were analyzed using one-way ANOVA and Duncan's multiple range test, and using chisquare test for microleakage values. The results were as follows: ·Polymerization shrinkage was increased with higher light intensity in groups using VIP^™(Bisco) : the highset with 600mW/cm^2, followed by Pulse-delay, 400mW/cm^2 and 200mW/cm^2 groups. The degree of polymerization shrinkage was higher with Continuous mode than with Ramp mode in groups using Optilux 501^™ (Demetron/Kerr). ·Degree of conversion and microhardness values were higher with higher light intensity. The final degree of conversion was in the range of 44.7 to 54.98% and the final microhardness value in the range of 34.10 to 56.340. ·Microleakage was greater in dentin margin than in enamel margin. Higher light intensity showed more microleakage in dentin margin in groups using VIP^™ (Bisco). The microleakage was the lowest with Continuous mode in enamel margin and with Ramp mode in dentin margin when Optilux 501^™ (Demetron/Kerr) was used.