양어용 사료첨가제로서 감귤발효액 (EM-Fermented Orange)의 항산화특성

문상욱,이영돈,이준백,고유봉 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

Mandarine orange (Citrus unshiu Marc.) only added with sugar was fermented by effective microorganisms (EM). called "EM-fermented orange". EM was mainly composed of lactic acid bacteria. phototrophic bacteria and yeast. As pH of EM-fermented orange reached 3.5 to 4.0 in the course of fermentation. the incubation was stopped. and then. EM-fermented orange was preservable at room temperature for about 6 months. Composition and concentration of free amino acids in EM-fermented orange were analyzed. Concentration of total amino acids was 167 mg/l. and the relative content of carnosine (β-alanyl-L-histidine) which was well known as a kind of antioxidant materials was very high. 41%. In addition. crude extract showing antioxidant activity could be obtained from EM-fermented orange using some organic solvents. The antioxidant activity of the crude extract was equivalent to about 9% of that of vitamin E by the use of 1.1-diphenyl-2-picrylhydrazyl (DPPH) method. It was suggested that EM-fermented orange having antioxidant characteristics could promote the health of fish.

膈下逐瘀湯과 膈下逐瘀湯合四君子湯의 抗癌 및 免疫調節作用에 關한 實驗的 硏究

崔昇勳,安圭錫,文濬典,高光錫 대한동의병리학회 1994 동의생리병리학회지 Vol.9 No.1

연구배경 : 암은 사망의 주요 원인이 되는 질환으로 매년 증가하고 있는 추세이다. 이에 mouse에 암을 유발하여, 약물치료를 시행함으로써 높은 치료효과를 거두는 한약제재를 개발하여 암의 치료에 기여하고 아울러 근본적인 예방치료에 대한 새로운 방법을 제시하고자 함. 방법 : 생쥐에 Sacroma 180을 이식하여 복강암을 유발하고 격하축어탕 및 격하축어탕합사군자탕을 투여하여 생명연장률과 B cell 및 T cell, 혈청항체가, 임파구증식반응, IL-2 생산능 및 자연살해페소의 활성도를 측정하였다. 결과 : 격하축어탕과 격하축어탕합사군자탕은 면역조절작용을 통한 항암효과가 있을 것으로 생각되며, 활혈거어제인 격하축어탕과 보기제인 사군자탕을 합방했을때, 그 효과가 증가되었다. In order to investigate the effects of Gyeokhachukeotang and Gyeokhachukeotang plus Sagunjatang on anti-tumor and immunodulatory reaction to the ICR and C57BL/6 mice which have abdominal tumor after Sarcoma-180 cells were transplanted, the extracts of herbal medicines above mentioned were orally administered to the mice for 21 days to observe the extention of survival duration of the mice, quantitation of T cells and B cells, productivitiy of interleukin-2, lymphocytes transformation, hemagglutinin titer and hemolysin titer and activity of splenic natural killer cells. The results are summarized as follows; 1. In the effect of life extention, the Gyeokhachukeotang-medicated mice group inclined to show extended duration of life, which is of no great significant difference and the mice group medicated with mixture of Gyeokhachukeotang plus Sagunjatang appeared to survive longer than the Gyeokhachukeotang-medicated mice group, which is not meaningful. 2. Considering T cell and B cell content, the Gyeokhachukeotang-medicated mice group had a meaningful increase in B cell (P<0.05) content, and the mice group medicated with the mixture of Gyeokhachukeotang plus Sagunjatang remarkably showed a significant increase in both T cell and B cell (P<0.01). 3. Considering serum antibody titer tended to increase in case of the Gyeokhachukeotang-medicated mice group, which is of no significant difference, while decreasing in case of the mice group medicated with mixture of Gyeokhachukeotang plus Sagunjatang. 4. In lymphocyte transformation marked a significant increase the Gyeokhachukeotang-medicated mice group, marked a significant (P<0.01), and remarkably showed a significant increase the mice group medicated with the mixture of Gyeokhachukeotang plus Sagunjatang (P<0.001). 5. As for the productivity of interleukin-2 and the activity of NK cells, the both tested groups inclined to show an increase or a decrese, but there is no significance.

65세 이상의 노인 환자에서의 심판막 폐쇄부전증에 대한 임상적 고찰

류주성,왕준호,문언수,이홍순 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

학교체육 활성화 방안에 대한 연구(Ⅰ) : 강원대학교 교양체육 운영방법의 개선을 중심으로

이광재,문병용,노성규,박기동,홍관이,한상준,유옥재,엄기진,정청자,오수일,김윤래,박장평,부기원 江原大學校附設 體育科學硏究所 1986 江原大學校附設體育科學硏究所論文集 Vol.- No.11

Try to find a scheme for activation to College Physical Education, this Report informs the various kinds of Survey and process those were projected for improvement of management methods on Cultural Physical Education in Kang Weon National University. From experimentally carried out "Sports events Choosing System" not as usual be inforced "Total practical Skill System", Following Positive effects and sujestions were Presented; 1. "Sports events choosing system" gave birth to more students' interesting and more voluntary participation to the sports events they chose, and sports skill and instruction level was elevated due to charge of expert according to each sports events. This system would be managed continuously hereafter for it related to life sports. 2. This System needs to closed cooperations between College and Community, for the facilities of community are utilized by college. 3. To complete the plan for activation of college Physical Education, additionally, the successional study ; namely "autonomous extracurricular sports activity" should be excuted hereafter.

손상정도계수와 외상치를 이용한 외상환자의 분석

송근정,장문준,김성중,조광현,김인병,최성욱,이한식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Trauma is one of the most common causes of death. Recently, injury severity score(ISS) is widely used to evaluate and classify the patients with multiple injury. Trauma score(TS) was developed as a means of providing rapid accurate assessment of the injured patient. Authors conducted a review of trauma patients who visited the emergency department of YongDong Severance Hospital from September 1992 to August 1993. ISS and TS were collected from comprehensive initial evaluation. The results were as follows : 1) The subjects were 10.2%(2,666 patients) of total 26,202 emergency patients. 2) Male to female ratio was 2.1 : 1. The age distribution was highest under 10 year(24.9%). The mean ISS was 3.5, and the mean ISS increased with ago. 3. Analysis of injury mechanism showed high incidence of traffic accident(35.3%), blunt trauma 25.7%, fall 24.2%, stabbing 10.7%, and others 4.3% in order. 4. The 8.0% of patients were admitted after initial management and their mean Iss was 12.6 mean TS was 15.5. 5. Among admitted traffic accident patients, the mean ISS of passenger T.A., pedestrian T.A. and driver T.A. were 19.0 ±9.9, 14.4 ±10.6, and 11.3 ±7.7 respectively. 6. Among 42 mortality cases, 25 patients were D.O.A. and 17 were expired. The mean ISS of D.O.A. Patients was 30.0; the mean Iss and mean Iss and mean TS of expired patients were 28.3 and 11.8 respectively. 7. The most common cause of death was head trauma(66.7%). 8. By comparing the ISS and TS of admitted and expired patients, mortality occurred above 16 point of ISS. The mortality increased with increasing ISS, but it was influenced by the trauma site and the severity of the particular injury site. Mortality also occurred below 15 point of TS, the mortality increased inversely with TS, and the mortality with TS, and the mortality was high below 13 point (above 40%).

음낭외상시 고환파열에 대한 초음파 검사의 진단적 유용성

김경영,한동준,신대은,노준,문형윤,임동훈,김철성,박성운 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Purpose: We determined the use of scrotal ultrasonography in the initial diagnosis and management of testicular injuries due to blunt scrotal trauma. Mateiials and Methods: We reviewed 54 patients of scrotal trauma in the last 8 years. Forty patients were evaluated by ultrasonography preoperatively. A heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition was the basis for diagnosis of testicular rupture. Among them, 33 cases underwent scrotal exploration and 7 cases were managed conservatively. The remaining 14 patients were evaluated only by physical examination, 8 patients of them were explored immediately because of suspicious testicular mpture. The accuracy of ultrasonography were determined by comparing radiographic interpretations with intraoperative findings. Results: Among the 33 patients who were evaluated by ultrasonography and explored immediately, there were 26 suspected testicular ruptures on ultrasonography. Surgical exploration revealed testicular rupture in 23 and simple hematoma in 10. The testicular rupture was correctly diagnosed by ultrasonography in the 22 cases, and there were 4 false-positive and 1 false-negative diagnoses of testicular rupture. The sensivity and specificity for the diagnosis of testicular rupture are 96% and 60%, and the positive and negative predictive values are 85% and 86%, respectively, Conclusions: Scrotal ultrasonography, with the main radiographic finding of a heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition, is highly sensitive on the diagnosis of testicular rupture. And it increases the salvage rate for testicular rupture with preservation of the testicular function and prevents delayed complications of chronic pain, atrophy and orchiectomy associated with missed testicular rupture.

Squalene Adjuvant를 이용한 단백질 항원의 세포성 면역반응 유도

도현주,김성열,안종성,하영주,이승찬,오재택,장명호,정홍석,문홍모,박해준 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

To investigate the role of adjuvant for cytotoxic T lymphocytes (CTL) induction, squalene-based adjuvant was studied its capability eliciting to the induction of cellular immunity as well as humoral immunity to exogeneous proteins. Ovalbumin (OVA) was used as model proteins. It was demonstrated that antigen formulation consisted of metabolizable oil squalene mixed with Tween 80 and pluronic L121 (S/TJ121) could induced the antigen specific CTL responses and antibodies irrespective of immunization routes in mice. We also demonstrated that this antigen formulation was a inducer of CD8+, major histocompatibility complex(MHC) class I-restricted, and antigen-specific C;1'Ls. These data suggest that the squalene-based emulsion system is a potent adjuvant inducing cellular immunity as well as humoral immunity.

암환자의 뇌혈관질환

이지용,권준범,양현덕,이성익,한범기,문준식,이성수 대한신경과학회 1999 대한신경과학회지 Vol.17 No.6

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

비파괴적 분석법을 활용한 활음진 함유 에멀젼의 인체효능평가

배지현,안성연,이해광,안순애,문성준,장이섭 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

최근 한방화장품이 각광을 받으면서 한방에서 말하는 피부개선효과에 대한 적절한 평가법을 찾는 연구가 많이 수행되고 있다. 본 연구에서는 피부 항산화 효과가 우수한 산사자, 당귀, 홍화, 두충, 숙지황의 초임계추출물 (활음진, 活陰眞)의 혈액순환 개선효과를 적외선 열화상 이미지 분석 및 LaserDoppler Perfusion Imager를 이용한 분석을 통해 평가하였다. 평소 손발이 차가운 여성 11명을 대상으로 활음진을 포함한 에멀젼을 1주일간 도포하게 하였으며, 도포전과 1주일간 사용한 후 피부 온도와 혈류량을 비교하였다. 그 결과 얼굴의 피부 온도가 증가하였고, 이마, 눈위, 뺨정면 부위에서 시료 사용 후 유의한 증가를 보였다. 본 연구를 통하여 활음진 성분을 포함한 에멀젼의 혈액순환 개선효과를 확인하였을 뿐만 아니라 한방적 접근을 통한 피부개선효과를 평가하는데 적절한 실험모델을 수립하였다.