http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
혈액응고 제8인자 유전자 내부의 BclI, Xbal RFLP 와 St14 VNTR 에 나타나는 다형인자의 빈도추정과 A 형 혈우병 8가계의 연관 분석
문홍모,김장성,오달균,강신혜 한국유전학회 1993 Genes & Genomics Vol.15 No.4
With the acid of PCR, we used two intragenic factor VIII gene RFLP markers(BclI-intron 18 and XbaI-intron 22) and one extragenic marker(St 14 VNTR) to estimate allele frequencies of these markers from 100 Korean individuals and to analyze eight Korean hemophilia A families. Allele frequencies for the presence of the enzyme sites and heterozygosity expectations were 86 and 24% for BclI-intron 18 RFLP marker, and 62% and 47% for XbaI-intron 22 RFLP marker, respectively. When BclI-intron 18 was homozygous, 31.5% of women was predicted to be heterozygous for XbaI-intron 22. Number of repeats in St14 VNTR loci from Korean males differed from those previously reported for Caucasian males. 68% of Korean women was estimated to be heterozygous for St14 VNTR marker. Analysis of hemophilia A families with BclI-RFLP and St14 VNTR proved to be informative for six out of eight families tested.
Microcarrier를 이용한 사람 폐세포에서 수두 바이러스의 증식
전복환,박손용,문홍모 대한바이러스학회 1994 Journal of Bacteriology and Virology Vol.24 No.1
A human embryonic lung fibroblast(LuMA) cell line was established for production of a live attenuated varicella-zoster virus vaccine. Using this cell line, we have investigated the possibili ty of using microcarrier culture system for producing cell-associated and cell-free varicellazoster virus. We have also investigated the utilization of glucose and amino acids during cell culture and virus propagation in microcarrier culture. It was found that no infectious cell-free viruses appear in the culture medium, and substantial decreases in cell density was observed a cell culture time increase. The cell-free varicella-zoster virus yield in microcarrier culture was much lower than that of the stationary T-flask culture.
유전자 재조합 대장균으로부터 인간 Interferon - α2 의 정제
이진규,강인철,박성희,정광희,문홍모 ( Jin Kyu Lee,In Chul Kang,Sung Hee Park,Kwang Hoe Chung,Hong Mo Moon ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1
Recombinant human IFN-α2 was purified from E. coli by methods involving sonication, extraction with 8 M Guanidine-HCI, dilution, CuSO₄ fractionation, copper-chelating column chromatography, S-Sepharose column chromatography. Specific activity of purified IFN-α2 was 1.6 × 10^9 IU/㎎ protein and the degree of purification was 94 fold from the starting material. Purified IFN-α2 was formed a single band on SDS-PAGE under reducing condition and also on non-reducing condition. Its molecular weight was estimated to be 18,000 dalton and the isoelectric point of purified IFN-α2 was 6.0. Purity of the recombinant IFN-α2 was better than 99% by densitometric analysis of SDS-PAGE.
Herpes Simplex Virus ( HSV ) 항원의 정제 및 HSV 항체 ( IgA , IgM ) 검사용 ELISA
조낙용,박해준,박송용,문홍모 대한바이러스학회 1993 Journal of Bacteriology and Virology Vol.23 No.2
The indirect enzyme-linked immunosorbent assay (ELISA) for detecting Herpes simplx virus (HSV) antibodies, IgG and IgM, was developed. HSV Type-1 strain MacIntyre, HSV Type-1 strain F, HSV Type-2 strain G and Herpes Type-2 strain MS were propagated on human embryonic lung fibroblast (MRC-5) cell. Viral antigens were extracted from culture media and purified by sucrose density gradient ultracentrifugation. Polystylene microwell plate was coated with purified HSV antigen (0.5 ug/well) and blocked with BSA solution. This assay system was capable of detecting HSV-specific IgG and IgM antibo dies in the sera in a short time (75 min). We compared this assay system with other commercially available EIA kits (Enzygnost;Behring, Germany). The overall sensitivity and specificity of IgG detection were 100% and 93.3 %, respectively. The sensitivity and specificity of IgM detection were 97.9% and 99.3%, respectively.