Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

Jeon Sang‐Jin,Kim Eun Joo,Oh Jihyun,Yun Chi‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.7

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells. Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

농업인 교육 프로그램 분석

김진모,김진군,마상진,전영욱,이진화,주현미 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구는 농업인을 대상으로 한 교육 프로그램의 내용 및 방법을 살펴봄으로써 향후 농업인 교육을 위한 기초자료를 제공하는 데 목적이 있었다. 농업인 교육프로그램 분석을 위한 개념적 틀은 관련문헌에 대한 고찰과 수집 자료에 대한 귀납적 분석을 통해 개발되었다. 그 결과, 8가지의 기관 유형, 17가지의 프로그램 내용 영역, 5가지의 교육방법, 3가지의 교육인원, 3가지의 교육기간이 도출되었다. 분석대상은 총 173개 기관의 2,483개 프로그램이었다. 이 연구의 주요 결과는 다음과 같다. 첫째, 우리나라의 농업인 교육은 국가기관 및 기술센터 등의 정부기관 주도로 이루어지고 있었다. 둘째, 재배/사육기술, 생활개선 및 농촌건강관리, 정보획득 등의 내용이 주요 교육내용으로 다루어지고 있었다. 셋째, 각 기관 유형별로 비중 있게 다루는 주요 교육내용에 있어서 다소 차이가 있었다. 넷째, 농업인 교육프로그램에서 활용되는 교육방법은 대체적으로 강의에 편중되어 있었다. 다섯째, 교육인원에 있어 중규모와 대규모 인원의 비중이 상대적으로 높았으며, 1 대 1 개별 교육은 전혀 없는 것으로 나타났다. 여섯째, 1일 혹은 3일 이상 이루어지는 교육의 비중이 상대적으로 높았으며, 2일 동안 이루어지는 교육의 비중은 비교적 낮은 편이었다. The purposes of this study were to analyze the farmer education programs in Korea, and to get implications for further program development. The framework for the analysis was developed through extensive literature review and an inductive method. As a result, eight types of organizations for farmer education, seventeen content domains of programs, five instructional methods, three categories of program participant size, and three categories of program period were derived. The subjects of analysis were 2,483 programs of 173 farmer education organizations. The major findings were as follows: 1) Farmer education programs of Korea were led by government agencies such as national organizations and extension agencies. 2) The content domains of cultivating/raising techniques, improvement of life quality and farmer health care, and information acquisition were relatively highly dealt with. 3) Main contents which were dealt with by each type of farmer education organization were differentiated. 4) In terms of instructional method, a lecture had the highest portion in most contents domains. 5) The portion of the middle size and large size of participants was relatively higher than others. And there are no programs which used one by one teaching method. 6) The portion of programs for 1 day or 3days was relatively higher, and the portion of programs for 2 days was lower.

개발사업 환경성평가에서의 토양 탄소저장량 산정방법 비교 연구

황상일 ( Sang Il Hwang ),박선환 ( Sun Hwan Park ),전영봉 ( Young Bong Jeon ),황정배 ( Joung Bae Hwang ),강선홍 ( Seon Hong Kang ),김진홍 ( Jin Hong Kim ) 한국환경영향평가학회 2014 환경영향평가 Vol.23 No.6

The objective of this study was to propose the best methodology for estimating soil organic carbon stocks during environmental assessment for development projects. We compared three methodologies which were developed by Korea Environment Corporation(2010), Korea Forest Research Institute (2006), and Jin-Hyun Jung (1998). We found that the methodology developed by Jin-Hyun Jung (1998) shows the worst performance and the methodology of Korea Forest Research Institute (2006) does not reflect a variety of soil types and land use characteristics shown in development project plans. Therefore, we propose that the methodology developed by Korea Environment Corporation (2010) is the most reasonable one because it is internationally accepted and used for local governments to make the inventory of greenhouse gases as well as to set up its reduction strategy.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

한국형 우울장애 약물치료 알고리듬 (Ⅳ) : 우울장애의 아형 및 부작용에 따른 항우울제의 선택과 여성우울장애에서의 치료전략

전현태,이상열,김원,민경준,박원명,서정석,석정호,송해철,전덕인,홍진표,한국형 우울장애 약물치료 알고리듬 2006 연구그룹 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Objectives : In 2002, the Korean Medication Algorithm Project for Major depressive Disorder (KMAP-MD) was published, but there has been a need for a guideline about detailed issues of depressive disorder. We revised KMAP-MDD andreestablished Korean Medication Algorithm Project for Depressive Disorder (KMAP-DD) in 2006. Methods : A questionnaire had been developed by the executive committee for KMAP-DD. The review committee consisted of 101 experienced psychiatrists. From the total of 22 questions in the questionnaire, 7 questions were evaluated for these subjects . We classified the expert opinions to 3 categories according to its confidence interval; first, second and third line. Results : SSRI and venlafaxine were the first line antidepressants (AD) for atypical and melancholic depression. For dysthymic disorder and minor depressive disorder, SSRI was recommended as the first line medications. Only AD medications was a preferred initial strategy for treating premenstrual dysphoric disorder, mild to moderate and severe non-psychotic postpartum depression. In severe psychotic postpartum depression, combination therapy of AD and atypical antipsychotics was the treatment of choice. SSRI was preferred when considering sedation, anticholinergic and cardiovascular adverse effects. Also, experts recommended mirtazapine against gastrointestinal adverse effects and bupropion in avoiding sexual dysfunction. Conclusion : These results suggest that clinicians have to consider both clinical situations and drug adverse effects in the choice of antidepressant medications.

항공기 개념설계를 위한 천음속 영역의 공력특성 예측에 관한 연구

전권수,김상진,이재우,변영환 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

During the aircraft conceptual design phase, rapid aerodynamic analyses over various configurations are required. Hence, empirical and analytic techniques play important roles in estimating the aerodynamic characteristics. In this study, the aerodynamic characteristic estimation technique for transonic region based on empirical and analytical is developed. By comparing with the results of the wind tunnel experiment and existing prediction method, it is verified that the present method is accurate and useful.

개방교합과 과개교합에서 구치의 근원심 치축경사도에 관한 두부방사선계측학적 연구

전상범,김진범,손우성 대한치과교정학회 1993 대한치과교정학회지 Vol.23 No.3

For the purpose of investigating mesiodistal axial inclination of posterior teeth in normal occlusion group, open bite and deep bite group and investigating the correlationship between the axial inclination of posterior teeth and overbite of anterior teeth, a cephalometric study was performed on the subjects consisted of normal occlusion group(40), open bite group(71 : Angle's Class Ⅰ 21, Class Ⅱ, division 1 25, Class Ⅲ 25) and deep bite group(64 : Angle's Class Ⅰ 23, Class Ⅱ, division 1 21, Class Ⅲ 20). Mesiodistal axial inclination of posterior teeth to occlusal, mandibular and palatal plane were measured. The findings of this study were as follows : 1. Upper and lower posterior teeth were more mesially inclined to occlusal plane in open bite group than in deep bite group. 2. Lower posterior teth were more mesially inclined in deep bite group than in open bite group in Angle's Class Ⅱ, division 1 malocclusion but there were no significant differences in Angle's Class Ⅰ and Class Ⅲ malocclusion. 3. There was no significant correlationship between the axial inclination of posterior teeth to each plane and overbite of anterior teeth in open bite group. 4. There was a significant correlationship between the axial inclination of upeer and lower second premolar to occlusal plane and overbite of anterior teeth in Angle's Class Ⅰ, Class Ⅱ, division 1 and Class Ⅲ malocclusion.

오존노출이 실험적으로 유발된 알레르기성 비염에 미치는 영향

전우진,변상내,김용민,김병국,나기상,박찬일 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

정신분열병 환자에서 세가지 청각 자극 "Oddball" 모형에 의한 사건관련전위 P3a와 P3b

진용탁,박이진,남지민,한상익,전양환 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : Using 3-stimulus auditory "oddball" paradigm reflecting fronto/central and temporo/parietal functions Simultaneously, we examined patients with schizophrenia. Methods : fifteen patients with schizophrenia from outpatient clinic and fifteen normal controls from hospital staffs were recruited for the study. To elicit P3a and P3b, 3-stimulus auditory oddball paradigm was employed. The 3-stimulus auditory oddball paradigm was composed of standard tone (1,000 Hz, 75 dB, 80%), target tone (2,000 Hz, 75 dB, 10%) and distracter (White noise, 95 dB, 10%). Results : P3a and P3b were prominent in fronto/central and temporo/parietal areas, respectively, in both schizophrenics and normal controls. The P300 amplitude in patients with schizophrenia was reduced across P3a and P3b (p<0.01). The P300latency in patients with schizophrenia was delayed across P3a and P3b (p<0.01). Conclusion : These results were consistent with frontal and temporo-parietal lobe dysfunctions in schizophrenics. The 3-stimulus auditory paradigm could be applied for patients with schizophrenia and useful for further exploration the disorder.

유아의 성별, 연령에 따른 체력 특성

전영남,양점홍,김수진,이성민 釜山大學校 附設 體育科學硏究所 2005 體育科學硏究所 論文集 Vol.21 No.2

The purpose of this study is to examine the characteristics of young children's fitness development in accordance with gheir gender and age, providing information necessary for an effective physical education instruction for young children's growth and development. For the purpose, this researcher selected total 257 young children from K Kindergarten located at K-gu in B Metropolitan City who consisted of 73 boys and 45 girls, both of were fully aged 4, and 83 boys and 56 girls, fully aged 5.......