Development of a Mobile Application, "Wild Flowers of Bukhansan National Park (version 1.0)", for Identification of Plants in Bukhansan National Park

Kim, Sang-Tae,Lee, Seung-Yeon,Kim, Seung-Chul,Byun, Hye-Won,Lee, Sang-Tae,Kim, Mu-Yeal,Hong, Seok-Pyo,Chung, Young-Jae,Park, Ki-Ryong,Lee, Chung-Hee,Lee, Joong-Ku,Heo, Kyeong-In,Lee, Ji-Ye,Lee, Eun-Je National Science Museum of Korea 2011 Journal of Korean nature Vol.4 No.3

We developed the educational purpose mobile application, named "Wild Flowers of Bukhansan National Park (version 1.0)", aiming for easy identification of wild flowers for students and visitors in the park. When visitors find a flower or part of plant in the park, visitors can search for its name utilizing the pictures and characters provided in their own smartphone mobile devices or tablet PCs. The application provides pictures of wild flowers in the park and character-based searching system based on 12 diagnostic features (e.g., growth form, leaf arrangement, flower symmetry, petal color, petal number, sepal number, etc). We adopted the complete floristic survey of Chung and Lee (1962) and added species that we confirmed their distribution in the park during the development of this application. In summary, number of vascular plants in this park was estimated to be 428 taxa including 100 families, 280 genera, 327 species, 1 subspecies, 50 varieties, and 5 formas. We provided a total of 588 pictures representing 358 taxa and each taxon includes multiple pictures in many cases. Included identification quizzes can be an efficient educational tool as well as fun activity for students and visitors who are learning plant species in Korea. Our next step will include GPS function in the application for indicating visitor's location and for providing previously reported sites of the species that we interested in the map of the park. The future application which includes GPS function will be a valuable tool for the monitoring of rare plants, plant researches related to the climate changes, etc. We currently provide Korean iPhone version only, and English version and both of android versions will be serviced soon.

리더십 행동의 규정요인 및 유효성 검토 : An Expectancy Theory Approach

임무경,김석일,박일호 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The purpose of this study was to examine the influence of the instrumentality perceptions (concerning the degree to which the leadership behavior is seen as leading to accomplish his own instructional objectives), extrinsic factors (characteristic of group, characteristics of followers, task uncertainty and favorableness for leader) and instrumentality perceptions (concerning the degree to which the leader is seen as leading to realize follower's objectives) on the leadership behavior (initiating structure and consideration), and the influence of the difference between two leadership measurements (leader's own and followers') on the effectiveness of sport clubs in community. The subjects were 54 leaders and 273 members of sport clubs in community. 1. The correlation between the instrumentality perceptions of Initiating structure and the actual leadership (initiating structure) were considerably high. 2. Significantly positive correlation between the instrumentality perceptions of leader by club members and the attitude toward the leadership of leader were found. 3. As to the effectiveness of clubs, coincidence clubs (leader's own evaluation and followers' on leadership) was higher than uncoincident clubs. These findings indicated that the leader's leadership behavior (initiating structure) could be predicated from the Expectancy Theory model and the difference between leader's own evaluation and that of club members' on leadership influenced on the effectiveness of sport clubs in community.

적조생물 살조세균 탐색 -IV. 살조세균 Micrococcus sp. LG-5가 생산하는 살조물질의 특성과 해양생물에 미치는 영향-

정성윤,박영태,김무찬,최석철,성희경,김재영,김태운,이원재,JEONG Seong-Youn,PARK Young-Tae,KIM Mu-Chan,CHOI Seok-Cheol,SEONG Hee-Kyung,KIM Jai-Young,KIM Tae-Un,LEE Won-Jae 한국수산과학회 2000 한국수산과학회지 Vol.33 No.4

적조생물을 살조시키는 해양세균 Microcorcus sp. LG-5가 생산하는 세포외 분비 살조물질의 최적 생성조건, 살조물질의 특성과 한외여과 분획별 살조활성 및 해양생물에 미치는 영향을 조사함으로서 자연생태 조화형, 환경 친화적 적조방제 기술개발의 기초 자료를 제공하고자 연구한 결과를 요약하면 다음과 같다. Micrococcus sp. LG-5의 살조물질 생성 최적온도는 $20{\~}30^{\circ}C$로 살조활성이 $100{\%}$였으며, 살조물질 생성 최적 pH는 7.0, 최저 염농도는 $3.0{\%}$였다. 또한 세포외 분비 살조물질의 $IC_(50)$ 값은 $0.482{\%}$였다. 세포외 분비 살조물질의 열 안정성을 실험한 결과, 열처리한 배양여과액 $1{\%}$ 첨가시 12시간 후에 $31.4{\%}$, 24시간 후 $79.7{\%}$, 36시간 후 $94.6{\%}$의 살조활성을 보였고 $5{\%}, 10{\%}$의 경우 각자 36시간, 24시간 후에 $100{\%}$ 살조되었으며, 열처리하지 않은 배양여과액을 넣었을 때와 비교해볼 때 살조력에 유의한 차이는 없었다. 이와 같이 Micrococcus sp. LG-5가 생산, 분비하는 살조물질은 열에 안정한 내열성 물질로 판단된다. 또한 대사산물의 pH 안정성은 $pH 6.0{\~}8.0$에서 거의 $100{\%}$의 살조활성을 보였으며 $pH 5.0{\~}10.0$에서 살조활성이 $85{\%}$ 이상으로 나타나 살조물질은 약산성과 알카리에서 살조활성이 안정하였다. 또한 한외여과의 결과, 분자량 $>10,000, 10,000{\~}1,000, <1,000$의 3개의 분획 모두 살조활성이 있었으며, 각 분획 모두 열에 대단히 안정하였다. 각 분획별 $IC_(50)$ 값은 분자량 1,000 이상에서 강한 살조활성이 나타났으며 분자량 1,000 이하에서는 상대적으로 낮은 살조활성을 보였다. Micrococcus sp. LG-5가 해양세균에 미치는 영향은 E. faecalis, E. coli, K. pneumoniae 및 V. alginolyticus에 대해서는 항균력을 보였으나, P. aeruginosa, P. fluorescens, S. typhi, S. aureus, V. cholerae 및 V parahaemolyticus에 대해서는 전혀 항균력을 보이지 않았다. 그리고 적조생물에 미치는 영향은 A. tamarense, E. gymnastica, G. catenatum, G. niikimotoi, G. sanguineum, G. impudicum, H. triquetra, H akashiwo, P. micans와 Pyramimonas sp.에 대해서는 살조활성을 보였으나 Chlamydomonas sp., C. closterium, P. mininum, P. triestimum, Pseudonitzschia sp.와 S. trochoidea에 대해서는 살조활성을 보이지 않았다. 즉, 적조생물에 따라 살조활성이 다양하게 나타나 종 특이성이 낮았다. 먹이생물에 미치는 영향은 1 galbana를 제외하고 실험한 모든 먹이생물에 별다른 영향이 없거나 오히려 성장을 촉진시켰고, 또한 살조세균 배양여과액을 넙치 사육수에 $10{\%}$까지 투여하였을 때 양식 넙치에 미치는 유해한 생리학적 영향은 없었다. 이상의 연구 결과 살조세균 Micrococcus sp. LG-5의 배양여과액은 조사된 적조생물을 제외한 해양세균, 치${\cdot}$자어의 먹이생물 및 양식 넙치에 유해한 영향은 미치지 않을 것으로 판단된다. An algicidal bacterium, Micrococcus sp. LG-5 against the harmful dinoflagellate, Cochlodinium polykrikoides was isolated. The optimal conditions for the highest algicidal activity of bacterial culture filtrate showed in the range of $20{\~}30^{\circ}C$, at pH 7.0 and $3.0{\%}$ of NaCl concentration. In addition, $IC_(50)(mean of 50{\%} inhibitory concentration)$ of the culture filtrate against C. polykrikoides after incubation of 5 days was $0.482{\%}$. To investigate heat and pH stability of the culture filtrate of Micrococcus sp. LG-5, the culture filtrate ($pore size, 0.1 {\mu}m$) was heated to $121^{\circ}C for 15 min$ and adjusted pH from 2.0 to 10.0. There were no significant changes in algicidal activity by heat treatment and the pH change between pH from 5.0 to 10.0. The algicidal substances produced from Micrococcus sp. LG-5 were mainly detected in the fraction of $10,000{\~}1,000$ MWCO (molecular weight cut-off). The culture filtrate of Micrococous sp. LG-5 showed antimicrobial activity against Enterococcus faecalis, Escheiichia coli, Uebsiella pneunioniae and Vibrio altinolyticus, but did not show against Pseudomonas aeminosa, P. Buorescens, Salmonella typhi, Staphylococcus aureus, V. cholerae and V parahaemolyicus. The culture filtrate of Micrococcus sp. LG-5 was examined against 16 phytoplankton species and showed the algicidal activity against Ajexandzium tuarense, Eutreptiella Drnnastin, Gymnodinium catenatum, G. mikimotoi, G. sanguineum, eyodinium impuaicum, Heterocapsa triquetra, Heterosipa akashiwo, Prorocentrum micans and Pyraminonas sp.. However no algicidal effects of Micrococcus sp. LG-5 were observed against Chlamydomonas sp., Cylindrotheoa closterium, P. mininum, P. triestimum, Pseudonieschia sp. and Sczipuiella trochoidea. On the other hand, algicidal activity on the tested marinelivefood was not detected except for Isochrysis galbana. In addition, physiological responses of cultured olive flounder (Paralichthys oliraceus) exposed to $1 and 10{\%}$ of the culture filtrate of Micrococcus sp. LG-5 were measured. There were no clear changes in AST, GGT, creatinine, urea, total cholesterol, total protein, albumine, $Mg^(+2), Ca^(+2), Na^+, K^+, and Cl^-$. These results indicate that olive flounders were not affected when they were exposed to the culture filtrate of Micrococcus sp. LG-5.

TFT -LCD 구동 IC용 커패시터 내장형 DC-DC 변환기 설계

임규호,강형근,이재형,손기성,조기석,백승면,성관영,이용진,박무훈,하판봉,김영희,Lim Gyu-Ho,Kang Hyung-Geun,Lee Jae-Hyung,Sohn Ki-Sung,Cho Ki-Seok,Baek Seung-Myun,Sung Kwan-Young,Li Long-Zhen,Park Mu-Hun,Ha Pan-Bong,Kim Young-Hee 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.7

A non-overlap boosted-clock charge pump(NBCCP) with internal pumping capacitor, an advantageous circuit from a minimizing point of TFT-LCD driver IC module, is proposed in this paper. By using the non-overlap boosted-clock swinging in 2VDC voltage, the number of pumping stages is reduced to half and a back current of pumping charge from charge pumping node to input stage is also prevented compared with conventional cross-coupled charge pump with internal pumping capacitor. As a result, pumping current of the proposed NBCCP circuit is increased more than conventional cross-coupled charge pump, and a layout area is decreased. A proposed DC-DC converter for TFT-LCD driver IC is designed with $0.18{\mu}m$ triple-well CMOS process and a test chip is in the marking. 본 논문에서는 TFT-LCD 구동 IC 모듈의 소형화측면에서 유리한 DC-DC 변환기 회로인 펌핑 커패시터 내장형 비중첩 부스트-클락 전하펌프 (Non-overlap Boosted-Clock Charge Pump: NBCCP) 회로가 제안되었다 .2VDC 전압으로 스윙하는 비중첩 부스트-클럭의 사용으로 기존의 펌핑 커패시터 내장형 크로스-커플드 전하펌프에 비해 펌핑 단의 수를 반으로 줄일 수 있었고, 전하 펌핑 노드의 펌핑된 전하가 입력 단으로 역류되는 현상을 방지하였다 . 그 결과 제안된 펌핑 커패시터 내장형 비중첩 부스트-클럭 전하펌프 회로는 기존의 펌핑 커패시터 내장형 크로스-커플드 전하펌프에 비해 펌핑 전류가 증가하였고, 레이아웃 면적은 감소되었다. 제안된 TFT-LCD 구동 IC용 DC-DC 변환기 회로를 $0.18{\mu}m$ Triple-Well CMOS 공정을 사용하여 설계하고, 테스트 칩을 제작 중에 있다.

Antipathogenic Properties of Green Tea Polyphenol Epigallocatechin Gallate at Concentrations below the MIC against Enterohemorrhagic Escherichia coli O157:H7

LEE, KANG-MU,KIM, WAN-SEOK,LIM, JEESUN,NAM, SUNYOUNG,YOUN, MIN,NAM, SEONG-WON,KIM, YOUNGHOON,KIM, SAE-HUN,PARK, WOOJUN,PARK, SUNGSU International Association for Food Protection 2009 Journal of food protection Vol.72 No.2

<P>The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μg/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.</P>

24년생 루브라참나무의 생장과 재질에 관한 연구 -물리,역학적 성질-

한무석 ( Mu Seok Han ),이창준 ( Chang Jun Lee ),박봉석 ( Bong Seok Park ),김병로 ( Byung Ro Kim ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

종자의 산지가 다른 루브라참나무(24년생)를 대상으로 물리 및 역학적 관계를 조사하여 생장속도와 목재의 재질과의 관계를 규명하였다. 생재함수율은 변재보다 심재가 높은 것으로 나타났고, 산지 간에 차이를 나타냈다. 비중의 경우 심재는 생장이 좋을수록 작아지는 경향을 보였고, 변재는 생장이 좋을수록 커지는 관계를 나타냈다. 전수축률은 산지 간 차이를 나타냈으며, 생장이 좋을수록 수축률과 T/R율은 높아지는 것으로 나타났다. 압축영계수, 휨강도와 휨영계수 및 충격강도는 산지간 차이를 보였으며, 생장이 좋을수록 낮아지는 경향을 보였다. 전단강도는 산지간 차이를 보였으며, 생장이 좋을수록 커지는 경향을 보였다. 경도는 세 단면에서 산지 간 차이를 보였으며, 생장이 좋을수록 커지는 경향을 나타냈다. 5종의 산지가 다른 루브라참나무 중 Bancroft산 종자의 경우가 가장 좋은 수고생장을 보이고, 세 번째의 흉고직경을 나타내며, 압축, 휨, 경도, 전단, 충격강도 모두 우수하여 생장과 재질면을 고려했을 때 현시점에서 가장 우수한 품종으로 판단된다. Relationship between growth rate and wood quality was investigated by physical and mechanical properties with Quercus rubra(24 years old) from five different origin of apricot. In greenwood moisture content, sapwood had higher moisture content than heartwood, and there was difference among different origin of apricot. There were different specific gravity of wood among different origin of apricot. Compared with higher growth rate with higher specific gravity in sapwood, opposite trend was observed in heartwood. There were difference in shrinkage based on origin of apricot, and higher growth rate wood had higher shrinkage and T/R ratio. Compression Young`s modulus, bending strength, bending Young`s modulus, and compact strength was difference among different origin of apricot. Higher growth rate wood had higher tensile strength, and also there was difference amont different origin of apricot. In hardness, 3 different directions had all difference among different origin of apricot, and higher growth rate wood showed higher hardness than others. Based on overall physical and/or mechanical properties and growth rate, apricot from Bancroft was best quality in current.

횡성댐 상하류의 홍수량 산정과 상류의 배수영향 분석

박석재(Park Seok Chae),정상만(Jeong Sang Man),박무종(Park Mu Jong),전병호(Jun Byung Ho) 대한토목학회 2000 대한토목학회 학술발표회 논문집 Vol.2000 No.3

IEC TS62600-201 조류발전 부존량평가: 한반도의 조류에너지 부존량 평가에 적용

박준석(Jun Seok Park),박진순(Jin Soon Park),이철용(Chol Young Lee),고동휘(Dong Hui Ko),최현우(Hyun Woo Choi),이진학(Jin Hak Yi),정무혜(Mu Hea Jung),서한경 한국해양환경·에너지학회 2018 한국해양환경공학회 학술대회논문집 Vol.2018 No.11

Estimation of Cellular Damages Caused by Paraquat and lead Using a Cell Culture System

Park, Young-Im,Noh, Eun-Woon,Han, Mu-Seok,Yi, Yong-Sub The Korean Society of Plant Biotechnology 2001 Plant molecular biology and biotechnology research Vol.3 No.2

A cell culture system of poplar (Populus alba x P.glandulosa) was established to test four different methods for evaluation of cellular stresses. Two different kinds of stresses were given to the cultures by adding either Pb(NO$_3$)$_2$ or paraquat and the cellular responses were monitored during a week period. While fresh weight reduction was observable in two days after the treatment of Pb(NO$_3$)$_2$, such changes were apparent only in later stage in paraquat treated cultures. Cells in paraquat treated cultures in the first 3 days showed no alteration in fresh weight as compared to untreated cultures, but had their MTT reducing activities completely inhibited. Neither Evans blue staining nor ion conductivity of the medium was consistent with fresh weight changes of the cultures. Overall, cell clumps formed during suspension culture appeared to interfere with staining and washing reactions and thus cause the assays unreliable. Among the four methods examined, fresh weight changes and MTT reducing activity appeared to be the most reliable and consistent.

면역기능이 정상인 환자에서의 십이지장 모균증 1예

박희승,이혜진,김우석,최성호,김무영,권태헌,한서룡,이중길 대한소화기내시경학회 2000 Clinical Endoscopy Vol.20 No.4

Mucormycosis is a rare and fulminating opportunistic fungal infection that occurs almost in immunocom-promised patients. It is also a highly virulent and rapidly progressive disease with poor prognosis. Its incidence has been increasing in recent years. We have experienced a case of solitary duodenal mucormycosis in a 45 year-old male patient. Mucormycosis was diagnosed by pathology & culture and he was treated with amphotericin B and discharged with clinical improvement. We report this case with a literature review.