Pt계 촉매를 이용한 직접 개미산 연료전지의 특성 연구

유재근,이효송,김진용,김기호,이영우 충남대학교 공업교육연구소 2005 論文集 Vol.27 No.1

Direct Formic Acid Fuel Cells (DFAFCs) are potential alternative power sources for potable devices such as cellular phones, personal digital assistants (PDA) and laptop computers. In this study, we investigated the effect of the anode catalysts on the performance of direct formic acid fuel cell. We made the Pt-Pd catalysts using deposition method for the investigation of the fuel cell performance as a change of temperature and oxidant. Fuel cell with Pt-Pd anode catalyst showed the performance of 120 mW/㎠at room temperature. This result was much higher value than that of Pt-Ru catalyst which showed the best performance of commercial catalyst in our previous works. For this reason, we can conclude that the Pt-Pd catalyst is an excellent material for the oxidation of formic acid.

사군자탕이 항암제를 투여한 마우스의 면역세포에 미치는 영향

殷載淳,金大根,柳東和,權鎭,洪鍾星,蘇俊魯,全焄,吳贊鎬 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

The purpose of this research was to investigate effects of Sa-Kunja-Tang(SKT) on immune cells of antitumor drugs administered mice. The apoptosis and T lymphocytes subpopulation were tested using a flow cytometry, and the proliferation was tested using a MTT assay. The administration of etoposide. vincristine or doxorubicin increased the apoptosis of T-lymphocytes, but the action of doxorubicin was decreased by the administration of SKT. The administration of etoposide or vincristine decreased helper T and cytotoxic T cells population of T lymphocytes, but the action of vincristine was recovered by the administration of SCT. The administration of etoposide, vincristine or doxorubicin decreased the proliferation of T-lymphocytes, but the action of doxorubicin was increased by the administration of SKT. These results suggest that SKT has a regulative function of T-lymphocytes in anti-tumor drugs administered mice.

Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석

이영근,장화형,장유신,허재호,형석원,정혜영 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3

본 연구에서는 감마선으로 유도된 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자들의 발현을 연구하기 위하여, B. lentimorbus WJ5의 방사선 유도 돌연변이체에서 발현되는 유전자를 DNA microarray로 동시에 탐색하였다. DNA microarray는 B. lentimorbus WJ5 genome을 무작위로 절단하여 2,000단편으로 구성하였으며, 감마선 (^(60)Co)으로 유도된 7돌연변이체의 발현을 정량적으로 관찰하였다. 클러스터 분석결과 발현된 408 유전자 중 27개가 감마선 유도 돌연변이체 모두에서 유의하게 발현이 증가되었다. 특히, 복구(mutL, mutM)에너지 대사 (acsA, sdhB, p하, yhjB, citB), protease(npr), 산화자극에 대한 환원 (HMM)관련 유전자들이 동시에 증가되었다. 이는 감마선 유도 돌연변이체들에서 자발적인 직/간접 복구 관련 유전자의 발현 증가는 방사선 노출 직후 보이는 stress response와는 다른 현상임을 나타내는 것으로 생각된다. To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA microarray. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation (^(60)Co). From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.

최근 8년간(2001-2008) 서울대학교치과병원 소아치과의 진료현황 및 수익분포 변화에 대한 조사

손유진,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

The practice pattern of pediatric dentistry in the university dental hospital has been changing for the last several decades, due to socioeconomic changes, developments in dental materials and increasing number of private pediatric dental clinics. The purpose of this study is to survey the changing patterns of pediatric dental treatment in the university dental hospital and to present the future direction for pediatric dentistry. Patient distribution and practice trends were reviewed based on the OCS (order communication system) records of the department of pediatric dentistry, Seoul National University Dental Hospital from 2001 to 2008. Results were as follows; 1. The number of patients increased up to 2005 and decreased in 2006, and has remained constant up to 2008. 2. The proportion of preventive and orthodontic treatment increased and the proportion of restorative and pulpal treatment decreased. 3. In restorative treatment, amalgam restoration decreased and resin restoration increased. 4. Frequency of sedation decreased in 2006, 2007 and increased in 2008. 5. Frequency of general anesthesia increased up to 2008. 소아치과 전문 지역 의원의 증가, 소아치과에 대한 보호자의 인식 변화, 치의학의 발전, 소아치과 진료 범주의 확대 등에 의해 최근 소아치과의 진료 양상이 크게 변화하고 있다. 이러한 변화 양상을 파악하고 앞으로의 대학병원 소아치과 및 소아치과 전문 지역 의원의 진료분담에 대한 방향성을 제시하고자 본 연구를 시행하였다. 2001년 1월 1일부터 2008년 12월 31일까지 서울대학교치과병원 소아치과에 내원한 모든 초진 및 재진 환자를 대상으로, 서울대학교치과병원 종합의료정보시스템(OCS)을 통하여 1년간의 외래환자집계 및 수가코드별 진료 행위 건수 및 수익 분포를 조사하여 다음과 같은 결과를 얻었다. 1. 내원환자의 수는 2001년부터 2005년까지 대체로 증가하다 2006년에 감소하였으며 그 이후에는 2006년도와 별다른 변화가 없었다. 2. 2001년에는 예방치료가 6.84%, 수복치료가 49.96%, 치수치료가 9.64%, 외과치료가 10.78%, 교정치료가 22.78%를 차지하였으나, 2008년에는 예방치료가 15.40%, 수복치료가 28.97%, 치수치료가 3.28%, 외과치료가 14.83%, 교정치료가 37.52%를 차지하여 수복치료와 치수치료의 비율은 감소하고 예방치료와 교정치료는 증가하였다. 3. 수복치료의 비율에 있어서 아말감과 글라스 아이오노머 수복 및 기성금관수복의 비율은 감소하였고, 레진의 비율은 증가하였다. 4. 진정요법하 치과치료는 2005년까지 증가하다 2006년, 2007년에는 감소하였고, 2008년에 다시 증가하였다. 5. 전신마취하 치과치료는 2001년부터 2008년까지 꾸준히 증가하였다.

이차원전기영동법을 이용한 길항세균 Bacillus licheniformis DM3와 이의 항진균 활성 결여 돌연변이균주간 단백질 비교 분석

이영근,김재성,정혜영,장유신,장병일 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3

항진균 활성균주를 선발하는 과정에서 DM3 균주를 대천 바닷가에서 수집된 진흙 시료로부터 분리하였으며 API 50CHB kit를 이용하여 동정한 결과 Bacillus licheniformis로 동정되었다. 이 균주는 12종의 식물병원성 진균에 대해 항균활성을 나타내었다. 감마선(^(60)Co)을 조사하여 항진균 활성 결핍 돌연변이체(mDM3)를 유도한 후, 이차원전기영동으로 단백질을 분석한 결과 DM3와 mDM3에만 존재하는 각각 4종과 3종의 단백질을 확인할 수 있었다. 2-DE 결과 B. licheniformis DM3에서 spot 1은 serine hydroxymethyltransferase(45.0 kDa), spot 2는 hypothetical protein(40.7 kDa), spot 3는 NifU protein homolog(15.4 kDa), 그리고 spot 4는 resolvase(12.5 kDa)와 상동성을 지닌 단백질로 동정되었고 B. licheniformis mDM3에서만 발현된 spot 5는 lysozyme(18.1 kDa)과 spot 6, 7은 alkyl hydroperoxide reductase(15.6 kDa)으로 동정되었다. B. licheniformis DM3에서 이들 단백질들의 항진균 활성 관련 여부를 규명하기 위해서 더 연구가 진행되어야 할 것으로 사료된다. In the course of screening for antifungal agents, a bacterial strain, DM3 was isolated from a mud sample collected at Daechon in Chungnam province and identified as Bacillus lichenifomris based on API 50CHB test. It has antifurgal activity against 12 plant pathogenic fungi in paper disc assay. At the 95% lethal dose of gamma radiation (^(60)Co, 10 kGy, D_(10)=2.32 kGy), the antifungal activity deficient mutant (mDM3) against Colletotrichum gloeosporioides was induced. From 2-D electrophoresis analysis, serine hydroxymethyltransferase(45.0 kDa), hypothetical protein(40.7 kDa), NifU protein homolog(l5.4 kDa), and resolvase(l2.5 kDa) horn ologous proteins were detected only in B. lichnifomris DM3. Lysozyme(l8.1 kDa) and dkyl hydroperoxide reductase(l5.6 kDa) homologous proteins were expressed uniquely in B. licheniformis mDM3. Further studies are needed to reveal that these proteins from B. lichenifomis DM3 could be closely related to the antifungal activity against plant pathogenic fungi.

골뱅이(Buccinum Undatum) 알레르기 : 감작률과 다른 음식물 항원과의 연관성 Sensitization rate and its relationship with other food allergens

이재영,윤성호,서유진,최정희,서창희,남동호,김윤근,민경업,박해심 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1

Background : The common whelk (Buccinum undatum) is one of the largest and most common snails in the North Atlantic. In Korea and Japan, common whelk is a popular eatable shellfish. Although shellfish has been known as the one of the most common causes of food allergy, there has been no published report on allergenecity and clinical significance of this sea snail. In this study, we determined the sensitization rate to common whelk and its relationship with other food allergens in allergy patients. Method : We carried out the skin prick test (SPT) with commonly consumed food stuffs in Korea including common whelk in 1700 patients over 1 year. The specific IgE to common whelk were detected by enzyme-linked immunosorbent assay (ELISA). ELISA inhibition test using sensitized sera was conducted. Results : SPT to common whelk was positive (≥2+) in 83 (4.9%) patients studied. Twenty-four (38.7%) of 62 SPT-positive patients had high serum specific IgE to common whelk. ELISA inhibition test showed significant inhibitions by abalone as well as by common whelk and, minimal inhibition were noted by shrimp. Significant correlation was also noted in specific IgE levels between common whelk and abalone (r=0.58, F<.05). Conclusion : IgE-sensitization rate to common whelk was 4.9% in allergy patients. Further stuides are needed to evaluate the clinical significance of sensitized patients to common whelk, and to confirm a cross reactivity with abalone. (J Asthma Allergy Immunol 24 : 77-84, 2004)

전립샘에 발생한 원발성 악성 림프종

기근홍,정유경,심재영 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.3

Primary malignant lymphoma of the prostate is extremely rare. This tumor should be rule out infiltration of lymphoma arising in the other site. We experience a case of diffuse large B-cell type of primary prostatic malignant lymphoma in an 70 year-old male. There is no extra-prostatic involvement III extensive staging work-up. Transrectal prostatic needle biopsy was performed. On microscopic examination, the atypical lymphocytes were diffusely infiltrated and replaced of the prostatic parenchyme. The atypical lymphocytes had scant cytoplasm and large nucleus with irregualr nuclear chromatin. Nucleoli were not distinct. Immunohistochernically, the tumor cells were reactive for LCA, CD20 and kappa light chain, but not reactive for CD3, UCHL-1 and Bcl-2. Histopathologic diagnosis is diffuse large B-cell lymphoma of the prostate.

6주간의 '내경일지선(內勁一指禪)' 기공수련이 대학생의 신체조성 및 심폐기능에 미치는 영향

오재근,유종만,이근배 한국체육대학교 1996 敎養敎育硏究所 論文集 Vol.- No.1

'소림내경일지선' 수련이 대학생의 체력과 운동수행능력에 미치는 영향을 규명하기 위하여 최근 3개월 동안 특정한 운동을 지속적으로 행한 경력이 없으며 의학적 검진상 이상이 없는 건강한 남녀 대학재학생 6명을 대상으로 6주간의 기공수련을 실시한 후, 트레이드밀 위에서 All-out에 도달할 때까지 심박수, 혈압, 산소섭취량, 최대산소섭취량의 변화와 체지방률, 체지방량, 제지방체중, 체수분의 변화를 관찰한 결과 다음과 같은 결론을 얻었다. 1. 운동전 안정시 심박수(HR), 이완기 혈압(SBP)과 수축기 혈압(DBP)의 변화는 기공수련전에 비해 기공수련후 낮아졌다. 또한 운동전 안정시 폐할량(VC), 강제 호기율(FEV)과 최대환기량(MVV)은 기공수련전에 비해 기공 수련후 증가하였다. 2. 운동시 심박수 변화는 기공수련후 측정시 기공수련전에 비해 증가률이 낮았으며, 운동후 휴식기 심박수 변화는 기공수련전보다 기공수련후 유의성 있게 낮아졌다. 3. 운동시 산소 섭취량(VO₂) 변화는 기공수련후 운동시와 휴식시가 기공수련전에 비해 증가하였다. 또한 최대산소섭취량(VO₂max)의 변화는 기공수련후가 기공수련전에 비해 증가하였다. 4. 기공수련 참여전ㆍ후의 체중(weight), 체지방률(percent body fat), 체지방량(fat body weight), 제지방체중(lean body mass) 및 체액(body water)의 변화는 모두 기공수련후가 기공수련전에 비해 감소하였으나 통계적인 의의는 나타나지 않았다. Nowadays Qigong is one of the universal health-promotion-methods in Korea and is becoming more popular among Koreans. But it is not accomplished to have scientific analyses in sports science on the effects of Qigong. Therefore, this study was attempted to determine whether 6 wk Qigion training affect on alteration of cardiopulmonary factors(heart rate, systolic blood pressure, diastolic blood pressure, vital capacity, forced expiratory volume in one sec., maximal voluntary ventilation), heart rate during exercise stress test, oxyge uptake & maximal oxygen uptake and body composition(weight, body fat, fat body weight, lean body mass, body fluid). As a result of this study, it aimed to investigate effects of 6wk Qigong training on cardiopulmonary function, physical fitness and body composition. For this study, 6 voluntary subjects(male 4, female 2) were chosen in untrained students of K University. they wre trained by teachers during 6 weeks and tested just before Qigong training and after 6 weeks. Each subject were measured body composition by body composition analyzer(model 310, biodynamics, U.S.A) and practiced pulmonary function test (model ST 250, Fukuda Sangyo Co., Japan) in regular order. And then they performed a treadmill exercise(model Q65, Quinton Co., U.S.A.) to the all-out state. During exercise stress test, electrocardiogram, heart rate and blood pressure were checked by stress test monitor(model Q4500, Quinton Co., U.S.A.) and also oxygen uptake, maximal oxygen uptake analyzed continuously by automatic gas analysis(model QMC, Quinton Co., U.S.A). Proc-T test of statistical analysis system was used in every experiment for statistical assessment. The results of proc-T test on this data were summarized as follow: 1. Cardiopulmonary function (HR, SBP, DBP, VC, FEV1, MVV) after Qigong traing were more improved than before 6 weeks(Table 1, Fig. 1). 2. Heart rate change during exercise stress test after Qigong training was shown more decreasing tendency than before training(Table 2, Fig. 2). Especially, heart rate change after Qigong training during resting periods was decreased significantly than before training. 3. Oxygen uptake change during exercise stress test after Qigong training was more increased than before training(Table 3, Fig. 3). And also maximal oxygen uptake after Qigong training was more increased than before 6 weeks(Table 4, Fig.4). 4. Body composition(Wt, body fat, FBW, LBM, body fluid) after Qigong training was more decreased than before 6 weeks(Table 5, Fig. 5). Above results indicate that Qigong training for 6 weeks has the effects on improving body composition and cardiovascular function.

暗渠排水에서 Grove의 公式에 關한 考察

周載洪,李裕根 진주산업대학교 1979 論文集 Vol.17 No.-

Grover's formula had played an important role to develop the theory for descent of the undergroundwater level in the underdrainage. Fall velocity of the underground water level gives a big effect to the cultivation of crops in the wet land. As a result of Grover's theory into pratice, Dr Yamazaki Hujio had found that was a big error. As applicating in experiment plots to Grover's fomula, obtained results are as follows. 1. As you see in the table(1), the result of experiment of Dr Yamazaki Hujio was existent higher value of v than the result of Grover's fomula. The result of fomula(Yc=127e-0.0496t) which resolved to apply a big value of v is existent a calculation mistake. it has been found a phenomenon that the water go not down into the underground, conversely going up. 2. As you see in the table(1),(2),(3) compared with Grover's fomula(1),(2) the value of y_c is a big error in regard to the value of t. 3. As you see in the table(3), (4) the result of applicating in my experiment plots, Grover's fomula is approximate with regard to the actual value.

Silorane-based 복합레진의 색안정성에 관한 연구

손유진,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구의 목적은 silorane-based 복합레진의 색안정성을 기존의 methacrylate-based 복합레진과 비교하여 평가하는 것이다. Silorane-based 복합레진인 P90(3M ESPE, USA), methacrylate-based 복합레진인 Tetric Ceram(Ivoclar Vivadent, Liechtenstein), Z250, Z350(3M ESPE, USA)을 이용하여 시편을 제작하고, 증류수, 커피, 레드 와인, 카레 용액에 담가 1일, 3일, 7일, 14일, 21일, 28일 후 색 변화를 분광광도계로 측정하고 실험 전후의 색상차이(ΔE*_(ab))값을 산출하여 다음과 같은 결과를 얻었다. 1. 카레 용액 > 레드 와인 > 커피 > 증류수의 순으로 레진 시편의 변색이 크게 나타났다. 2. 네 가지 종류의 레진을 비교하였을 때, Z350 > Z250 > Tetric Ceram > P90의 순으로 변색이 크게 나타났다. 3. 카레 용액의 경우 네 종류의 레진 모두에서 침지 1일 이후 수용도 역치 이상(ΔE*_(a)b>3.3)의 변색이 나타났다. 4. 레드 와인의 경우 Z350, Z250, Tetric Ceram에서 침지 1일 이후, P90에서는 침지 28일 이후에 수용도 역치 이상의 변색이 나타났다. 5. 커피의 경우 Z350에서 침지 1일 이후, Z250에서 침지 3일 이후, Tetric Ceram에서 침지 7일 이후에 수용도 역치 이상의 변색이 나타났으나 P90에서는 침지 28일 이후에도 수용도 역치 이상의 변색이 나타나지 않았다. The objective of this study was to evaluate the color stability of new silorane-based composite resin compared with methacrylate-based composite resins after immersion in 3 staining solutions. One silorane-based composite(Filtek P90) and 3 methacrylate -based composites(Filtek Z250, Filtek Z350, Tetric Ceram) were evaluated. Twenty disk-shaped specimens(10×2mm) of each of 4 composite resins were prepared. The specimens were then divided into 4 groups of 5 specimens each and immersed in 3 staining solutions( coffee, red wine, curry solution) or distilled water(control) for 28-day test period. Color of the specimens was measured with a spectrophotometer(Color Eye 7000A) using CIE L*a*b* color space relative to CIE standard illuminant D65 at baseline, 1day, 3days, 7days, 14days, 21days and 28days after staining. The color differences( ΔE*_(ab)) were calculated. The results were obtained as follows ; 1. The mean color changes(ΔE*_(ab)) of the composites were greatest in curry solution, then red wine, coffee and distilled water, in decreasing order. 2. The mean color changes(ΔE*_(ab)) of the composites were greatest in Z350, Z250, Tetric Ceram and P90, in decreasing order.