Reversine induces caspase-dependent apoptosis of human osteosarcoma cells through extrinsic and intrinsic apoptotic signaling pathways

김재성,조인아,강경록,임향기,김태현,유선경,김흥중,이슬아,문성민,전홍성,김춘성,김도경 한국유전학회 2019 Genes & Genomics Vol.41 No.6

Background The 2-(4-morpholinoanilino)-6-cyclohexylaminopurine (reversine) acts as a chemopreventive agent and induces apoptotic cell death in various cancer cells. However, the anticancer effects of reversine on osteosarcoma cells are not clearly established. Objective The purpose of this study was to investigate the effect of reversine on cell proliferation and induction of apoptosis in human osteosarcoma cells. Methods Cell viability assay, histological analysis, DAPI staining, caspase activation analysis, flow cytometric analysis and immunoblotting were carried out in MG-63 osteosarcoma cells. Results Reversine inhibited the growth of cells in a dose-dependent manner and induced nuclear condensation and fragmentation. Reversine-treated cells showed caspase-3/7 activation and increased apoptosis versus control cells. FasL, a death ligand associated with extrinsic apoptotic signaling pathways, was significantly up-regulated by reversine treatment. Moreover, the caspase-8, a part of the extrinsic apoptotic pathway, was activated by reversine treatments. Expressions of anti-apoptotic factors such as Bcl-2 and Bcl-xL, components of the mitochondria dependent intrinsic apoptosis pathway, significantly decreased following reversine treatment. The expressions of pro-apoptotic factors such as BAX, BAD and caspase-9 increased by reversine treatments. In addition, reversine activated caspase-3 and Poly (ADP-ribose) polymerase (PARP) to induce cell death. The Z-VAD-fmk significantly inhibited cell death through the suppression of caspase-3 expression in MG-63 cells treated with reversine. Conclusion These results suggest that the reversine may inhibit cell proliferation and induce apoptotic cell death in MG-63 osteosarcoma cells through both the mitochondria-mediated intrinsic pathway and the death receptor-mediated extrinsic pathway, and may have potential properties for the discovery of anti-cancer agents.

소프트웨어 거래의 UCC 제2편의 적용에 관한 연구 - 미국의 판례를 중심으로 -

김재성,Kim, Jae-Seong 한국무역상무학회 2002 貿易商務硏究 Vol.18 No.-

Owing to a tremendous progress of information technology we have faced a great change of a society. This change has happened to almost all of a society. As a result intangible goods are also included as a major concern in international trade. We may say U.S. is one of the countries transaction of software is the most flourishing. I have tried to classified the cases of software transaction in U.S. into five groups. As a result I have known the UCC had been applied to the almost transaction of standard software. However UCC had not been applied to custom software and data processing. In these days UCITA which is very similar to a previous proposal UCC 2B has been presented. This is a contract law that would apply to computer software, multimedia products, and databases. It has been designed to create a uniform commercial contract law for those products. Now therefore we should make observation of a tendency about transaction of software in U.S. The reason is that we pay attention to the symbol and dormant power of U.S. in international trade.

MicroRNA-205 Directly Regulates the Tumor Suppressor, Interleukin-24, in Human KB Oral Cancer Cells

김재성,김도경,유선경,이명화,박민경,박으뜸,김수관,이숙영,김춘성,김현중,전홍성,천상우,김흥중 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.1

MicroRNA (miRNA) is a form of small noncoding RNA that regulates the expression of genes either by inhibiting mRNA translation or by inducing its degradation. Small microRNA play important roles in regulating a large number of cellular processes, including development, proliferation and apoptosis. This study examined the biological functions of miR-205 as a tumor suppressor in KB oral cancer cells. The results showed that miR-205 expression was significantly lower in KB oral cancer cells than in human normal oral keratinocytes. Furthermore, the miR-205 over-expressed in KB oral cancer cells increased the cell cytotoxicity and induced apoptosis through the activation of caspase-3/-7. The transfection of miR-205 into KB oral cancer cells strongly induced IL-24, a well known cytokine that acts as a tumor suppressor in a range of tumor tissues. In addition, miR-205 targeted the IL-24 promoter directly to induce gene expression. Overall, miR-205 has significant therapeutic potential to turn on silenced tumor suppressor genes by targeting them with miRNA.

인터넷 전자상거래 계약성립의 일반원칙에 관한 소고

김재성 한국무역상무학회 2000 貿易商務硏究 Vol.13 No.-

[신박한 신약성경] “잘했다! 착하고 신실한 종아” : 마태복음 25:19-27

김재성 새가정사 2021 새가정 Vol.739 No.-

목재포장용기 및 깔판류제조업 경영실태 분석

김재성,주린원 한국임학회 2008 한국임학회 학술발표논문집 Vol.2008 No.-

선치료(Zen Therapy) 어떻게 볼 것인가? - 불교학자의 입장에서 -

김재성 한국불교상담학회 2013 한국불교상담학회 학술대회지 Vol.6 No.-

생로병사와 구부득고(求不得苦), 애별이고(愛別離苦), 원증회고(怨憎會苦), 오취온고(五取蘊 苦)라는 고통의 문제를 근본적으로 해결하는 것을 목적으로 하는 불교는 간단히 말하면 심리적 문제에 대한 치유법이자 궁극적인 해결법이라고 할 수 있다. 서양의 심리치료/상담도 인간의 정서적, 심리적 문제에 대한 치유를 목적으로 하는 심리학의 한 분야이다. 불교와 서양의 심리 치료는 인간의 심리적 문제의 이해와 해결이라는 공통의 과제를 가지고 있다고 볼 수 있다. 전통적인 불교 문화권에서는 불교 교학과 수행을 통해 인간의 성숙과 깨달음을 추구해오고 있다. 한편 서양에 전해진 불교는 서양 심리학과 심리치료와 불교 교학과 수행을 서로 접목하 여 현대인의 심리적 문제를 치유하는데 응용하려는 시도를 해왔다. 정신분석 또는 정신역동적 인 입장과 불교의 접목, 인지행동치료와 불교의 접목, 인본주의 심리치료와 불교의 접목, 그 리고 자아초월 심리학/심리치료와 불교의 접목이 20세기 중반 이후부터 시도되어 왔다.

예수의 기적, 합리주의적 사고로 조명할 수 있을까?

김재성 한신대학교 한신신학연구소 2014 신학연구 Vol.51 No.1

The supernatural approach to miracles is a traditional perspective of the Church. This view is contrary to the naturalistic approach of the Rationalist perspective. Today, the problem is a combination of these two approaches. It is an approach which regards the miracle of Jesus as a divine, transcendent miracle while trying to explain it reasonably. It is also a perspective of the positive thinking or self-improvement industry, which originated in the United States. In this article, we review the points of Barbara Ehrenreich, focusing on her book, Bright-Sided. It is an excellent critique of the positive thinking and self-improvement industry in America. Ehrenreich confronts the false promises of positive thinking. Suffering from breast cancer herself, she realized that positive thinking is not helpful in curing that disease. She explores the self-improvement and positive thinking industry, discovering its symbiotic relationship with American capitalism. And she explores how poverty, obesity, and unemployment are being marketed as obstacles that can be overcome with a positive mindset. Ehrenreich sees the dark roots of positive thinking emerging from 19th-century religious movements. Mary Baker Eddy, William James, and Norman Vincent Peale paved the path for today’s self-improvement industry and positive psychology institutes. This positive thinking has had a major impact on the Korean church. Some churches regard it as a kind of gospel. As Ehrenreich has correctly pointed out, it is just another kind of self-improvement industry, and has nothing to do with the gospel or miracle of Jesus. In this mindset, God is no longer God; he is nothing more than a ‘supply system’ feeding the needs of human beings.

"십자가에 달리신 그리스도를 전하는 교회" □ 요 1:1-18 □

김재성 한일장신대학교 한국성경연구원 1999 성경연구(설교자를 위한) Vol.5 No.2

Primary Radiation Therapy of Polymorhic Reticulosis

김재성,윤형근,안용찬,박찬일,Kim Jae Sung,Yun Hyoung Geun,Ahn Yong Chan,Park Charn Il The Korean Society for Radiation Oncology 1991 Radiation Oncology Journal Vol.9 No.2

From 1979 to 1987, 33 patients with polymorphic reticulosis (PMR) limited in the upper airway were treated with primary radiation therapy and the analysis of treatment results was undertaken. Systemic symptoms such as fever, night sweats, and weight ioss were noted in $48\%$. The nasal cavity was most frequently involved $(85\%)$, although involvement of PNS $(33\%)$ and palate $(30\%)$ was not uncommon. The 5 and 10 year actuarial survival rates were $47\%\;and\;40\%$, respectively. The difference in NSD between patients with in-field failure and those without it was significant statistically. Also, field size was significantly smaller in patients with marginal failure than those without it. During the follow-up period, systemic failure was found in S patients (diffuse histiocytic Iymphoma in 4, histiocytic medullary reticulosis in 1). In this study, we can suggest that total radiation dose of at least 4500 cGy with generous treatment volume should be delivered to achieve better local control and that the developement of an effective systemic chemotherapeutic regimen is required to improve the survival after systemic relapse. 1979년부터 1987년까지 서울대학교 병원에서 상기도에 국한된 다형성 세망증으로 근치적 방사선치료를 받은 33명의 환자의 치료성적을 분석하였다. 발열, 체중감소, 도한등의 전신적 증상은 대상 환자중 $48\%$에서 나타났으며 침윤병소로는 비강이 $85\%$로 가장 많았고 븐비동 및 구개부가 각각 $33\%$와 $30\%$이었다. 전체 환자의 5년 및 10년 생존율은 각각 $47\%$와 $40\%$이었다. 방사선 조사야 내에서 재발한 경우와 그렇지 않은 경우간의 방사선 선량 차이는 통계학적으로 유의하였으며 조사야 경계부위에서 재발한 경우는 그렇지 않은 경우에 비해서 조사야 면적이 작았다. 추적 관찰 기간중 악성 임파종 4예, 골수 세망증 1예 등의 전신적 전이가 확인되었다. 따라서 방사선 치료를 시행하여 다형성 세망증의 국소 치료율은 향상시키기 위해서는 최소 4,500 cGy의 선량과 병소주위의 조직을 충분히 포함하는 조사야를 사용해야 하며 전신적 전이를 효과적으로 치료할 화학요법 면에서의 개선이 필요하다고 판단된다.