한국인 알코올의존환자에서의 제1형 부신피질자극 호르몬 분비 촉진 호르몬수용체의 유전자 다형성에 관한 연구

김철민,김성곤,김지훈,김현경,김미경,유정현,민은정 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

스트레스 반응에 중요한 역할을 하고 있는 CRHR1 유전자 SNP와 알코올 의존과의 연관성에 관한 선행 연구들이 있다. 이에 본 연구는 백인을 대상으로 조사했던 기존연구와는 달리 한국인 남녀를 대상으로 하여 알코올 의존군과 정상 대조군의 CRHR1 유전자형 빈도 및 대립유전자형 빈도를 비교하였다. 본 연구의 연구대상자들은 알코올 의존군 268명(남자 218명, 여자 50명)과 정상 대조군 137명(남자 78명, 여자 59명)이었다. CRHR1 유전자 SNP를 SNP database와 선행연구를 근거로 선정한 후, 연구 대상자들의 이러한 SNP 유전자형을 PCRRFLP방법으로 조사하였다. 그 결과, 한국인의 CRHR1 유전자 SNPs로 7개(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887)가 분석되었다. 이중 rs28364027의 A 대립 유전자의 빈도는 남녀 각각에서 알코올 의존군이 대조군보다 유의하게 높았다. 그리고 rs28364027의 AA 유전자형 및 A 대립 유전자와 rs878886의 CC유전자형 및 C 대립유전자의 빈도는 알코올 의존군내에서 여성이 남성보다 유의하게 높았다. 이러한 결과를 종합해 보면 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인의 중요한 요인일 가능성을 제시하고 있다. 또 이러한 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인에서 남녀간 차이를 설명할 수 있을 가능성도 시사하고 있다. Objectives:There were several preceding studies investigating the association between alcohol dependence and corticotropine releasing hormone receptor1(CRHR1) gene SNPs that play an important role in response to stress. The frequencies of CRHR1 genotypes and alleles were compared between alcohol-dependent patients and normal control subjects. Methods:The subjects were 268 alcohol-dependent patients(218 males, 50 females) and 137 normal controls(78 male, 59 female). CRHR1 gene SNPs were investigated according to the SNP database and the results from previous studies, and their genotypes were analyzed by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP). Results:Seven CRHR1 gene SNPs(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887) were found in Korean subjects. The frequency of rs28364027 A allele was significantly higher in alcohol-dependent patients than normal controls in both genders. In alcohol-dependent patients, the frequencies of AA genotype and A allele of rs28364027, and of CC genotype and C allele of rs878886 were higher in female than male. Conclusion:These results suggest that CRHR1 gene SNP is one of the important genetic factors in the etiology of alcohol dependence. And also it is supposed that the different frequency of SNP genotype could explain stress-related gender difference in the genetic etiology of alcohol dependence.

뇌혈관질환을 동반한 당뇨병 환자에서 지속적 혈당측정의 유용성 평가

정성훈,강인구,박철영,류미숙,우정택,김성운,김진우,김영설 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

연구배경: 뇌혈관질환을 동반한 고령의 당뇨병환자는 이들의 신경학적 결손으로 경고 증상없이 심한 저혈당에 노출될 가능성이 크다. 실제로 하루 4번 정도의 자가 혈당 측정으로는 이를 감지하기 어려울 뿐 아니라 검사에 따른 고통으로 적극적인 혈당 조절에 제한이 따르게 된다. 이에 저자들은 Mini Med사의 지속적 혈당측정기(CGMS, continuous glucose monitoring system)를 이용하여 뇌혈관질환을 동반한 당뇨병 환자에서 24시간 혈당의 변동양상을 알아보고 각기 다른 인슐린 치료방침에 따른 혈당조절의 효율성을 비교하고자 하였다. 방법: 2001년 1월부터 6월까지 경희대학교 부속병원에 입원해 있는 10명의 뇌혈관질환을 동반한 제 2형 당뇨병환자를 대상으로 하였다. 이 들은 모두 튜브식이를 받고 있엇고 최소 1개월 이상 인슐린 치료를 시행 받았다. 지속적 혈당측정기를 착용하고 3일간의 연속적인 혈당을 측정하였고, 치료 첫 날은 NPH, 둘째 날은 속효성 인슐린과 NPH, 마지막 날은 혼합형 인슐린제제로 치료하여 그 효과를 비교하였다. 이들 각기 다른 인슐린 제재의 하루 총량에 차이는 없었다. 혈당치가 3.3mmol/L 미만이거나 7.8mmol/L를 초과하는 경우 그 차이값의 총합을 시간으로 나누어 이 값(△Glu)이 적을수록 치료효과가 우수한 것으로 판정하였다. 또한 당 수치가 3.3mmol/L 미만인 경우를 저혈당 event, 16.7mmol/L 초과한 경우를 고혈당 event로 임의로 정의하였고, 이를 통해 지속적 혈당측정의 유용성을 간접적으로 평가하고자 하였다. 결과: 평균 △Glu값은 첫째 날이 0.93 ± 0.43mmol/Lㆍmin?¹, 둘째날이 0.71 ± 0.29mmol/Lㆍmin?¹ 마지막 날이 0.58 ± 0.29mmol/Lㆍmin?¹로서 서로 다른 3가지 치료방침사이에 통계학적으로 유의한 차이는 없었다(p=0.115). 10명의 환자들 중 자가 혈당측정기로는 1명에서 저혈당 event, 2명에서 고혈당 event를 인지하였으나 지속적 혈당측정기로는 각각 9명에서 event를 확인하였다. 결론: 지속적 혈당측정기를 이용한 각각의 인슐린 치료방침에 따른 혈당조절 효율성의 차이는 없었으나, 자가혈당 측정기로는 알 수 없었던 일 중 혈당 변동사항을 정확하게 알 수 있어, 뇌혈관질환을 동반한 당뇨병 환자에서 보다 적극적인 인슐린 치료 및 관리를 할 수 있을 것으로 생각된다. Background: Diabetic stroke patients are susceptible to hypoglycemia. However, there are many limitations in detecting hypoglycemic events, even though glucoses levels are checked 3 to 4 times per day using the fingerstick method. Therefore, we investigated the glycemic excursions and pattern in diabetic stroke patients using the continuous glucose monitoring system (CGMS, MiniMed) and its clinical utility. The other objective was to compare the treatment dfficacy between insulin regimens. Methods: From jan 2001 to jun 2001, 10 adult type 2 diabetic stroke patients wore CGMS for consecutive 3 days, which continuously checked the glucose level. NPH insulin, multiple daily injection of regular insulin ± NPH and the mixed type(Novolet 30/70, Novo Nordisk) were used on the first, second and third days of the study, respectively. If the mean delta glucose(△Glu = A+B/time, A=∑(hlucose-7.8), glucose > 7.8 mmol/l, B=∑(3.3-glucose) if, glucose < 3.3 mmol/l) was calculated in order to recognize the degree of individual glycemic changes and to compare the efficacy of each treatment diverse insulin regimen. We arbitrarily defined a glucose level less than 3.3mmol/l, as a hypoglycemic event, and more than 16.7mmol/l as a hyperglycemic event. Results: Variable patterns of glycemic changes were observed among patients with the same dosage, but a different insulin regimen. The mean △Glu was 0.93±0.43 on the first day, 0.71±0.29 on the second day and 0.58±0.29 on the third. There was no significant difference in treatment efficacy between the 3 different insulin modalities(p=0.115). Of the 10 patients, a hypoglycemic event was detected in 1 with the fingerstick method, in contrast to 9 with CGMS, with hyperglycemic events being detedted in 2 and 9, respectively. Conclusion: Continuous glucose monitoring maybe useful in providing the information necessary for optimal glycemic control in the diabetic stroke patients.

혈관형성 조절물질 탐색을 위한 제브라피쉬 동물모델의 이용

소주훈,최태영,권병목,김철희 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

The zebrafish (Danio rerio) is now the prominent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. Zebrafish embryos provide many advantages for experimental and genetic analysis of vascular development. In this study we describe the use of zebrafish model system for the experimental study of angiogenesis. We established several technical methods to define cellular and molecular alterations in vascular development; whole-mount in situ hybridization, microangiography, and alkaline phosphatase staining. We also introduced the overexpression experiments by using in vitro-transcribed synthetic mRNAs and vascular-specific gene promoters. For the gene knock-down analyses, we challenged morpholino antisense oligonucleotide system. With these efforts, the zebrafish might be used as a powerful tool for the screening of effective angiogenic factors.

運動前·後 靑少年의 身體構成과 尿成分 變化에 관한 硏究

鄭琮勳,朴哲浩,朴相甲 東亞大學校附設스포츠科學硏究所 1987 스포츠科學硏究論文集 Vol.5 No.-

As the subjects of this study, 5 non athletes, 5 Obesity Student, 5 hand ball players were among the high school boys to evaluate physical fitness. The biochemistric changes of urine were observed ofter maxexercise load by Bicycle Ergometer. The conclusition obtained is as follows; 1. Before exercise, % fat was measured 18.5 ±3.97 in non athletes, 42.9 ±2.2 in obesity student and 17.4 ±1.69% in hand ball players. 2. After exercise, urine protein was significantly increased by 1.48mg/dl (36.59%: P<0.05) in non athletes, 0.60mg/dl (46.23%: P<0.02) in Obesity student, and 1.57mg/dl (48.91%: P<0.05) in hand ball players. 3. And Urine acid was significantly increased by 17.84mg/dl (18.43%: P<0.02) in nonathletes, 23.43mg/dl (26.82%: P<0.01) in obesity student, and 9.15mg/dl (7.53%: P<0.02) in hand ball players. 4. Urine nitrogen was significantly increased by 194.4mg/dl (33.71%: P<0.001) in nonathletes, 168.00mg/dl (17.42: P<0.001) in obesity student, and 213.60mg/dl (24.92%: P<0.05) in handball players. 5. Urine creatinine was significantly increased by 15.58mg/dl (41.12%: P<0.001) in nonathletes, 72.91mg/dl (35.83%: P<0.02) in obesity student, and 43.07mg/dl in hand ball players. 6. Urine specific gaovity was increased by 0.5mg/dl (0.49%: P<0.005) in nonathletes, 0.004mg/dl (0.35%: P<0.001) in obesity student, 0.003mg/dl (0.25%: P<0.01) in hand ball players.

學問의 自由와 大學의 自治

河喆永,李東勳 東義大學校 法學硏究所 1988 공공정책연구 Vol.4 No.-

리튬이차전지용 부극재료로서 은분말에 대한 전기화학적 특성

박철완,도칠훈,문성인,윤문수 한국공업화학회 2003 응용화학 Vol.7 No.2

Silver powders have been extensively studied as an anode active material for lithium secondary batteries. In the present study, a unique attempt is made to develop silver negative electrode by slurry method, because the silver electrode is a good candidate to replace the commercial graphite electrode for lithium rechargeable batteries. Silver electrode was prepared and its electrochemical properties were characterized gradual increasing test state of charge (GISOC) analysis method. Charge-discharge performance indicates that the initial charge potential range of Li/Ag cells has showed similar behaviors compared with Li/Graphite system and the irreversible capacity facd is also higher than Li/Graphite system.

전자상점 성과에 관한 이론적 모형 구축

윤철호,김상훈 한국경영과학회 2003 經營 科學 Vol.20 No.2

This study developed the ESM (Electronic Store Success Model) by basing upon the revised D&M IS Success Model and reflecting the characteristics of marketing and e business. The theoretical rationale of this model was as following :1) Though the revised D&M IS Success Model (2002) must be more logical and complete model than the original D&M IS Success Model (1992), it did not reflect characteristics of marketing and e business. 2) In the marketing studies, Service Quality has been suggested as one of basic strategies for success. 3) In e business studies, Trust has been emerging as a potentially important antecedent of e loyalty. 4) There has been a paradigm shift from satisfaction to loyalty in marketing emphasis. 5) Nowadays, ERP (Enterprise Resource Planning) has been pervaded in many organizations and has supported PCA (Profit center Accounting) or Channel based Accounting, so it has not been difficult to evaluate financial Derformance of Electronic Store. The ESM that has six dimensions (System Quality, Information Quality, Service Ouality. Trust, Loyalty, Financial Performance) and expresses the Causal relationship among them was proposed and was compared with BSC (Balanced Scorecard). WebQual, and EWEM (Extended Web Assessment Method). The results showed that the ESM coincides with BSC and contains dimensions of WebQual, and EWEM.

마찰시험기를 이용한 치과교정용 설측 브라켓과 호선의 마찰력 비교

박지훈,이용근,임범순,김철위 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.1

Frictional force tends to rapidly increase as the relative angle between the bracket slot and the archwire increases beyond critical contact angle. The purpose of this study was to measure the frictional forces between lingual orthodontic brackets and orthodontic archwires with a friction tester (FPR 2000, Rhesca, Japan). The influence of the presence of artificial saliva was determined additionally. Two different lingual brackets (FJT: Fujita, Japan; ORM: Ormco, USA) and one labial standard bracket (CTL:TOMY, Japan) as a control were used in this study. Archwires of three different alloys (SS: Stainless steel, Ormco, USA; TM: β-Titanium, Ormco, USA; EL: Cobalt-chrome, RMO, USA) with 0.016 x 0.022 inch and 0.017 x 0.025 inch were used in this study. Each bracket-archwire couple was tested with a friction tester in the dry and artificial saliva environments. Each measurement was conducted with a angular velocity of 0.1 rpm for 90 seconds under the normal force of 100gf at 25 ℃ for the dry and 34 ℃ for the artificial saliva environment. For SS and EL archwires, frictional force was greater with FJT bracket than with ORM bracket (p<0.01). Compared with SS and TM archwires, 0.016 x 0.022 inch EL archwire showed the greatest frictional forces with two lingual brackets (p<0.01). There were significant differences in frictional force between the dry and artificial saliva environment (p<0.05), however, the effects of artificial saliva were different depending on the bracket-archwire combinations. Experimental critical contact angles were greater than theoretically calculated values.

치성 각화낭에서 매복치 동반 여부에 따른 Ki-67 발현 비교

박철우,김도경,안상건,김수관,윤정훈 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

Odontogenic keratocysts(OKCs) are frequently associated with erupted or impacted tooth. In such instances, the radiographic features simulate those of a dentigerous cyst. The purpose of this study was to evaluate a comparative immunohistochemical expression of Ki-67 as a proliferative marker in the OKC associated with or without impacted tooth. In addition, we have also been investigated with regard to the proliferative activity comparing the unilocular and multilocular varieties of the OKC. The material for this study consisted of thirty-two cases of OKCs (OKC with impacted tooth, n=16; OKC without impacted tooth, n=16) and ten cases of dentigerous cysts as a comparison. The results revealed that the proliferative activity of OKCs with impacted tooth was higher than those of dentigerous cysts. However, there was no correlation between Ki-67 immunoreactivity and association with or without impacted tooth in 32 cases of OKCs. In addition, this present study showed that there was no correlation between the unilocular and multilocular varieties of the OKCs imn proliferative activity.

과도한 운동으로 발생한 횡문근융해증에 의한 급성신부전증 1예

문철호,류보열,모성환,김태종,기장도,박용관,김용환,정종훈 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Rhabdomyolysis may be defined as a clinical and laboratory syndrome which releases muscle cell contents into the plasma, such as creatine phosphokinase, due to skeletal muscle injuries. It can be the result of many diverse causes including muscle disease, ischemia, genetic disorder, metabolic disorder, infectious disease, drugs, comatose state, trauma or vigorous exercise that can lead to acute renal failure as a complication. Since first reported in 1941, rhabdomyolysis-induced acute renal failure has been documented many times, and the major causes of this disease are carbon monoxide poisoning, snake bites, and etc in Korea Reports of acute renal failure after exercise-related rhabdomyolysis with myoglobinemia are rare. We report a case of acute renal failure caused by exercise-related rhabdomyolysis in a 20 year old man.