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소주훈,최태영,권병목,김철희 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2
The zebrafish (Danio rerio) is now the prominent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. Zebrafish embryos provide many advantages for experimental and genetic analysis of vascular development. In this study we describe the use of zebrafish model system for the experimental study of angiogenesis. We established several technical methods to define cellular and molecular alterations in vascular development; whole-mount in situ hybridization, microangiography, and alkaline phosphatase staining. We also introduced the overexpression experiments by using in vitro-transcribed synthetic mRNAs and vascular-specific gene promoters. For the gene knock-down analyses, we challenged morpholino antisense oligonucleotide system. With these efforts, the zebrafish might be used as a powerful tool for the screening of effective angiogenic factors.