부천집단의 타액내 Proline-rich protein(Pr)과 Double band protein(Db)의 다형현상에 대한 연구

이하규,이희천,이주희 가톨릭대학교 자연과학연구소 2003 자연과학논문집 Vol.24 No.-

부천 집단의 타액내 단백질 다형현상과 유전자 빈도를 조사하기 위해 135명의 부천 상도초등학교를 대상으로 polyacrylamide gel을 사용하여 전기영동한 후 DMB(3, 3′-dimethoxy-benzidine) 염색을 하였다. 그 결과 Pr 1-1, Pr 1-2와 Pr 2-2 세 종류의 표현형질이 나타났으며, Pr 1-1은 80명, Pr 1-2는 32명, Pr 2-2는 6명으로 나타나 이들의 유전자 빈도는 Pr¹=0.75, Pr²=0.25가 되었고, Double band proein은 Db(+)와 DB(-) 두 종류의 표현형질이 나타났으며 Db(+)는 13명, Db(-)는 122명으로 나타나 이들의 유전자 빈도는 Db^(+)=0.05, Db^(-)=0.95가 되었다. 이러한 유전자 빈도를 중국인 집단과 일본인 집단의 유전자 빈도와 비교해 본 결과 한국인 집단은 혈액뿐만 아니라 타액단백질에서도 중국인 집단과 일본인 집단의 중간위치임을 알 수 있었다. The polymorphism of phenotypes and the allele frequencies of salivary Proline-rich protein and double band protein were suited on some unrelated individuals of Bucheon population of Korea. Three types of Proline-rich protein was observed : (Pr 1-1, Pr 1-2 and Pr 2-2) and two types of Double band protein was observed(Db^(+) and Db^(-)). The gene frequencies of them were as follows; Pr¹=0.75, Pr²=0.25, Db^(+)=0.05, Db^(-)=0.95.

토양중 게르마늄 농도에 따른 벼의 생육 특성 및 게르마늄 흡수

이성태,이영한,최용조,이상대,이춘희,허종수 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

게르마늄의 약리효능이 알려짐에 따라 게르마늄이 깅화된 기능성 농산물의 요구도가 높아지는 추세이다. 본 연구는 게르마늄의 농업적 이용에 대한 기초 사료를 제공하고자 실시하였으며, 벼의 생육 및 게르마늄 흡수에 미치는 게르마늄처리 효과는 다음과 같다. 게르마늄을 0, 2.5, 5.0, 7.5 및 10.0 mg/kg으로 처리할 토양을 와그너포트에 넣어 벼를 재배힌 결과, 게르마늄 처리농도가 증가할수록 게르마늄 독성의 증가로 초장, 수장, 수수 및 수량이 급격히 감소하였으며 게르마늄 2.5 mg/kg 이상에서는 게르마늄 독성이 발생하였다. 게르마늄 처리농도가 증가할수록 벼의 게르마늄 흡수랑은 증가하였으나 벼의 게르마늄 이용율은 게르마늄 2.5, 5.0, 7.5 mg/kg 처리에서 각각 20.7, 12.5 및 7.5%로서 게르마늄 처리농도가 증가할수록 감소하였다. 벼의 부위별 게르마늄 함량은 볏짚>왕겨>현미 순으로 높았다. 게르마늄 2.5 mg/kg 처리시 볏짚, 왕겨 및 현미의 게르마늄 함량은 각각 103.4, 30.2 및 3.02 mg/kg 이었고, 볏짚과 왕겨에서는 게르마늄 무처리에서도 각각 2.9 및 3.9 mg/kg을 함유하고 있었다. 쌀의 아미노산 함량을 분석한 결과 게르마늄 처리농도가 증가할수록 Asx., Thr., Ser. 등 대부분 종류의 아미노산 함량이 증가하였으며, 그 결과 현미중 질소흡수량도 증가하였다. In order to obtain the basic information for agricultural utilization of Germanium(Ge), the growth characteristics and Ge absorption of rice plant were investigated with different Ge concentration in soil. Ge concentrations were treated with 0, 2.5, 5.0 7.5 and 10.0 mg/kg in pot(1/5,000a), respectively. As higher the Ge concentration in soil, the Ge absorption amount in straw, husk and brown rice were increased. But the yields were decreased with the increase of Ge phytotoxicity. When rice plant was grown more than 2.5 mg/kg Ge(as GeO₂) in the soil, growth was inhibited by germanium phytotoxicity and necrosis spots were observed in the rice leaf blades. Therefore the optimum concentration of Ge was less than 2.5 mg/kg in rice plant. When rice plant was cultivated on soil supplemented with 2.5 mg/kg Ge, Ge content in straw, husk and brown rice was 103.4, 30.2 and 3.02 mg/kg, respectively. The Ge content in plant was high in the order of straw > husk > brown rice. Most of the amino acids in rice were increased with the increase of Ge treatment, besides, total amino acid contents also increased.

패스트푸드점의 서비스품질과 만족에 관한 실증적 연구 : 대구지역을 중심으로

박진영, 이희천 김천대학교 1998 김천대학교 논문집 Vol.19 No.-

The objective of this study is to find the components of the service quality in fastfood industry and to examine the impact of service quality over customer satisfaction. Also it is the intention of the researchers to find the difference in evaluation of service quality using demographic variables. Firstly, preceding studies such as Dr. Munkyu Lee's indicate that service quality is comprised of 5 factors. However, this study showed 4 factors responsiveness-assurance, tangibility-reliability, empathy. benefits through empirical study. This findings show more relevance of this study over Dr. Lee as Dr. Lee's study focused on the different customer satisfaction between Korean customers and American customers while this study concentrated only on the Korean customers' satisfaction. Secondly, this study analysed the impact of service quality over customer satisfaction. Results show the components of service quality affecting customer satisfaction lie in tangibility-reliability and benefits. In view of the results findings, customer satisfactions are affected by visible factors, reliable factors and benefit factors such as waiting time, location, price, etc. Thirdly, this study examined the difference in evaluation of service quality according to the demographical characteristics. Result findings after empirical study show significant difference in statistics of demographics in all dimensions except responsiveness-assurance Service quality was highly evaluated by college students while it was lowery evaluated by less aged high school students. Monthly allowance shows significant difference in statistics except the empathy, while it is no difference in the resident style. As a result, there is some difference​​

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.

Gene pyramiding effect of two major bakanae disease resistance gene using qBK1 and qBKWD

Sais-Beul Lee,Tae-Heon Kim,Su-Min Jo,Jun-Hyun Cho,Ji-Yoon Lee,Jong-Hee Lee2,Dongjin Shin,You-Chun Song,Myung-Kyu Oh,Dong-Soo Park 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

전류 비유 모델에 의한 초등학생의 전류 개념 변화 분석

유병길,강인석,김병철,남만희,박선희,소하연,윤희정,이영아,이하룡,전병문,정승호 부산교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.25 No.-

There exists a major impediment to learning electricity which may illuminate why direct presentation of scientists' ideas is too aggressive a strategy. This impediment lies in thinking that electricity is used up and in envisaging the light bulb as a consumer. This view is based on a powerful idea which summarises much of our experience; it declares that 'things get used up', 'you cannot get something for nothing', and 'intuitive thinking'. To replace this view with scientific view, we made the analogy model. It is suggested that the analogy model is more effective in teaching electricity circuits than conventional teaching method.

Genetic Characterization of Haemagglutinin 1 Domain of Influenza B Viruses Isolated in Korea during $1988{\sim}1999$

Shin, Gu-Choul,Na, Byoung-Kuk,Lee, Joo-Yeon,Ahn, Jung-Bae,Park, Jong-Won,Lee, Jin-Soo,Kim, Jee-Hee,Kim, Woo-Joo,Kang, Chun The Korean Society for Microbiology 2003 Journal of Bacteriology and Virology Vol.33 No.4

Although Korean influenza virus isolates have been genetically associated with the vaccine strains of the corresponding year, influenza B viruses have prevailed almost every year in Korea during the past decades. We have 'analyzed the genetic characteristics and evolutionary patterns of the haemagglutinin (HA) 1 domains of influenza B viruses isolated during 1988-1999 using direct RT-PCR and sequencing. Phylogenetic analysis of influenza B viruses isolated in Korea indicated that antigenically and genetically distinguishable strains of the lineage II and lineage III variants had been cocirculated. Variants prevailed in early 1990s are represented in 1996/97 and 1998/99 and some different variants have been co circulated geographically and prevailed concurrently in Korea. All HA1s of Korean isolates have amino acid substitutions mainly in the region between position 124 and 310, which was previously proposed an immunodominant region. Insertion-deletion patterns of the HA gene revealed that Korean influenza B viruses were evolved from Lee40 with different manner between lineage II and III viruses. Lineage III viruses were also divided into two groups as conserved group and inserted group, in relation to Lee40. But lineage II viruses had evolved with directional pattern. Antigenic index proposed that influenza B isolates prevailed since 1996/97 seasons might had emerged from the antigenic variants of a Seo1697-like virus and that new variants might appear from the lineage II viruses resulting in persistent prevalence in Korea.

장애인생활시설의 교육서비스 요구 분석

이상복,김춘희,유은정,이원령,구원옥 대구대학교 특수교육재활과학연구소 2004 특수교육재활과학연구 Vol.43 No.1

The purpose of this study was to provide suggestions for the future 2year educational services based on the on-going education services for the persons with disabilities in the facilities. The results of the study were as follows. First, the facilities manly provided the persons with disabilities with the educational programs for improving basic functional skills in their daily living lives. Second, the recording data showed that average 2 out of 10 short-term plans were reached to above 80% of successful performance levels, and the rest of the 8 plans were reached to between 11% and 79% of the unsatisfactory levels.

1989-2000년의 5, 12세 한국아동의 치아우식실태에 관한 조사연구

이춘희,최충호,권호근 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.1

The objective of this study was to analize the change of dental caries prevalence in Korean children and adolescents from 1989 to 2000. National oral examination survey reports in 1989,1991,1995 and 2000 were collected for this purpose The major finding were summerized and the results were as follows : 1. The dft of 5-year-old children in creased from 1989 to 1995 and decreased slightly from 1995 to 2000 the dt decreased or remained constant and the ft increased. 2. The dt of 5-year-olds decreased constantly by 1991 in rural area but in urban area, it remained stable by 1991 and the ft increased in rural and urban area. 3. The DMFT of 12-year-old children increased from 1989 to 2000 the DT decreased of remanined constant and the FT increased, the MT remained below 0.05 4. The DT index of 12-year-olds decreased constantly by 1991 in rural area but in urban area, it remained stable by 1991 and FT index increased in rural and urban area 5. The percentage of caries free and caries experience have increased in rural and urban area. According to results the dft of 5-year-olds and the DMFT of 12-year-olds have increased specially among the component of dft and DMFT dt and DT decreased or remain stable while ft and FT increased constantly which means that the restorative index(ft/dft, FT/DMFT) increased also and the percentage of caries experience increased. Based on this study, preventive approach is more required to lower caries prevalence of korean childeren