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      • KCI등재

        갈매보리수나무 종자의 저장기간에 따른 발아 및 생리적 특성

        최충호,양병훈 한국자원식물학회 2015 한국자원식물학회지 Vol.28 No.4

        Germination properties, leachate electrical conductivity (EC), and inorganic compound leaching were analyzed to ascertain the storage ability and change of physiological characteristics during storage of Hippophae rhamnoides seeds. Seeds were placed in an incubator at 25℃ and sown in different soil media (sand, vermiculite and horticultural substrate) after being stored for 6, 18 and 30 months at 2℃. All germination properties decreased in accordance to an increase of the seed storage period. Compared with the seed storage for 18 months, germination percentage (GP), germination performance index (GPI), and germination value (GV) of seeds stored for 30 months decreased by more than 50%. When the seeds were sown in different soil media in a greenhouse, those germination properties were similar to the seeds germinated in an incubator, and mean germination time, GPI and GV had a significant difference except GP among soil media. EC and inorganic ion concentration had a strong positive correlation with the seed storage period, but the ratios of inorganic ions from stored seeds revealed that K + /Mg 2+ and Na + /Mg 2+ were inversely correlated with the storage period. 갈매보리수나무의 재배확대를 위한 대량생산을 목적으로 종자 저장성을 구명하고, 이에 따른 생리적 특성을 분석하였다. 종자를 6, 18, 30개월 저장한 후 발아특성을 조사한 결과, 기내치상의 경우 모든 발아특성에서 18개월 이후 큰 폭으로 감소하는 경향을 보여주었는데, 발아율, 발아균일지수, 발아치는 30 개월 저장했을 때 18개월 대비 50% 이상 크게 낮아졌다. 특히, 발아치의 경우 감소율이 75.9%로 가장 컸다. 온실 내 토양 종류별로 파종한 경우, 발아특성은 기내 치상과 마찬가지로 저장기간에 따라 감소하는 경향을 보였다. 토양종류 간에는 발아율을제외하고는 평균발아일수, 발아균일지수, 발아치 모두 차이를나타내었다. 종자 저장기간에 따른 전기전도도 및 무기이온(K+, Na+, Mg2+, Ca2+) 함량은 저장 기간이 늘어날수록 증가하는 경향을 보였다. 무기이온 비율의 경우 K+/Mg2+, K+/Ca2+, Ca2+/Mg2+, Na+/Mg2+는 저장기간이 길어질수록 감소하는 경향을 나타낸 반면, K+/Na+, Ca2+/Na+는 뚜렷한 경향을 나타내지않았다. 또한 종자 저장기간은 발아특성과 높은 부(-)의 상관을, 전기전도도 및 무기이온 함량과 높은 정(+)의 상관을 나타내었다. 무기이온 비율은 K+/Mg2+, Na+/Mg2+만이 높은 부(-) 의 상관을 나타내었다. 결과적으로, 갈매보리수나무 종자는 저장기간에 따라 발아특성이 급격히 감소하며, 이에 수반된 생리적 특성 또한 변화가 심하여 장기보존 대책이 필요한 것으로 사료되었다

      • KCI등재

        침수 및 예냉처리가 황벽나무의 종자 발아에 미치는 영향

        최충호,서병수 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.2

        The seeds of Phellodendron amurense have difficulty to obtain because these plants show dioecism with independent distributions. This experiment was conducted to find the effects of soaking and prechilling treatment on the germination of P. amurense seeds. Seeds were soaked for 3, 5 and 10 days (soaking treatment), and placed on petri-dish at 4℃ for 10, 20, 30 and 50 days after soaked at room temperature for 24 hours (prechilling treatment). After the treatments, germination percentage, mean germination time, germination speed and germination performance index were analyzed. As a result of sowing, prechilled seeds for 30 days had the highest percent of germination, whereas all of the soaked seeds showed lower percent of germination than non-treatment (control). In case of mean germination time, only prechilling for 30 days was more effective than control. In germination speed, all of the soaked seeds had lower values than control whereas all prechilled seeds except prechilled seeds for 20 days showed higher values than control. Especially prechilled seeds for 30 days showed the highest rate among the prechilling treatments. Germination performance index was similar to germination speed. 본 연구는 주로 독립수로 분포하여 생육하고 있는 황벽나무의 종자를 다량으로 보급하기 위한 효과적인 실생 번 식법으로서 침수 및 예냉 처리를 통해 발아율을 향상시키 고자 실시되었다. 침수처리는 실온에서 각각 3, 5, 10일 동안 실시되었고, 예냉 처리는 실온에서 24시간 흡습시킨 후 4℃ 저장고에 각각 10, 20, 30, 50일간 보관하여 실시하였다. 파종실험 결과, 황벽나무 종자는 예냉 30일 처리에서 발 아율 향상에 효과적이었으며, 침수처리는 오히려 발아율을 저해하는 요인으로 작용하였다. 평균 발아일수의 경우 예 냉 30일 처리만이 발아일수 단축에 효과적이었다. 발아속 도에 있어서는 침수 처리의 경우 모두 무처리구 보다 낮게 나타난 반면 예냉 처리는 20일 처리를 제외하고는 모두 무 처리구 보다 높게 나타났다. 특히 예냉 30일 처리에서는 현저히 높을 수치를 나타내었다. 발아균일지수 역시 발아 속도와 유사한 경향을 보였는데, 침수처리는 모두 낮게 나 타났으며, 예냉 30일 처리에서 가장 높은 수치를 보였다.

      • KCI등재

        갈매보리수나무 종자의 온도 및 여러 가지 전처리에 따른 발아반응

        최충호 한국자원식물학회 2012 한국자원식물학회지 Vol.25 No.1

        This study was carried out to test seed germination responses to temperatures and pre-treatments in Hippophae rhamnoides, which has many abilities in antioxidant activity, soil improvement and erosion control. H. rhamnoides seeds were placed at 10, 15, 20, 25, 30 and 35℃ under light condition. As the results, germination percentage (GP) was the highest at 15 and 20℃, and mean germination time (MGT), germination rate (GR) and germination value (GV) were the highest at 25℃. Quadratic and linear regression model were used to determine the cardinal temperatures such as base (Tb), maximum (Tm) and optimum (To) temperature for germination. In quadratic regression model using PG, Tb, Tm and To was estimated as 0.6, 36.4 and 18.5℃, respectively, and temperature range for germination was 35.8℃. In linear regression model using GR, Tb, Tm and To was estimated as 8.3, 35.4 and 25.3℃, respectively, and temperature range for germination was 27.2℃. Germination properties were investigated after H. rhamnoides seeds were treated by prechilling (1, 2, 4, 6 and 8 weeks),stratification (2, 4, 6 and 8 weeks), solid matrix priming (seed : carrier : water = 5 : 1 : 7, 8, 9 and 10), osmo-priming (-0.25,-0.5, -1.0 and -1.5 MPa) and calcium chloride (CaCl2) -priming (100, 200, 300 and 400 mM). The highest GP was observed in CaCl2 300 and 400 mM treatments, and MGT was the shortest in stratification 6 and 8 weeks treatments. GR and GV were the highest and GP was the second highest when seeds were prechilled for 1 and 2 weeks. Consequently, prechilling 1 or 2weeks treatment was considered as the appropriate method when we contemplate qualitative and quantitative effects in seedling production. 본 연구는 기능성 작물, 사방용 수종, 토양개량 등 활용성이 뛰어난 갈매보리수나무의 유용 유전자원의 장기보존및 실생번식에 도움을 주고자 종자의 발아 온도조건 및 전처리에 대한 발아특성을 구명하고자 실시되었다. 갈매보리수나무 종자는 10~35℃의 온도조건에 치상한 결과, 15,20℃에서 발아율이 가장 높았으며, 평균발아일수, 발아속도 및 발아치는 25℃에서 가장 좋은 결과를 나타내었다. 또한 주요 발아온도를 예측하기 위하여 2차 및 선형 회귀분석모델을 이용하였는데, 발아율을 이용한 2차 회귀분석모델에서는 기준온도 0.6℃, 최대온도 36.4℃, 적정온도18.5℃로 나타났으며, 발아가능 온도범위는 35.8℃이었다. 발아속도를 이용한 선형 회귀분석모델에서는 기준온도8.3℃, 최대온도 35.4℃, 적정온도 25.3℃로 나타났으며,발아가능 온도범위는 27.2℃로 분석모델간 차이를 나타내었다. 갈매보리수나무 종자를 생리적 처리 방법인 예냉, 층적및 priming을 이용하여 전처리한 후 발아특성을 조사한 결과, 발아율에서는 CaCl2 300, 400 mM priming 처리구에서 가장 높은 수치를 나타내었다. 평균발아일수는 층적 6,8주 처리구에서 가장 짧게 나타났으나 대조구 보다 발아율이 낮은 것으로 보아 발아일수의 단축 보다는 발아의 종기종료로 판단되었다. 발아속도 및 발아치의 경우 예냉 1, 2주처리구에서 가장 높게 나타났으며 발아율 또한 CaCl2 300,400 mM 처리구에 이어 높은 수치를 나타내어 유묘 생산시양적․질적 측면을 고려했을 때 가장 적정한 조건으로 판단되었다.

      • KCI등재

        응급의료시설의 공간구성요소에 관한 건축계획적 연구

        최충호,박재승 한국의료복지시설학회 2008 의료·복지 건축 Vol.14 No.2

        The purpose of this study was to spatial organization feature and area composition of the over 500beds general hospital that will be expected construct in Seoul, Gyeonggi-do area after 2000year. From this study, we can suggest architectural planning for the efficiency operating of emergency medical facilities that can be used as preliminary data. The Spatial organization of emergency medical facilities are categorize into 4 session as medical examination and treatment, diagonosticradiology, staff, public and the others. We can find spatial organization system and area composition that compare area composition of 6 hospital the subject of research in a emergency medical facilities. The result of this study were as follows : spatial distribution of its field, grasping of spatial organization factor and recognize its merits and demerits should be researched in advance. Through this kind of recognizing emergency system, realistic medical local and condition architectural planning for spatial organization will be operate.

      • KCI등재후보

        일부 산취급 근로자의 치아부식증 실태 및 발생에 영향을 미치는 요인

        최충호,김백일,권호근 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.4

        The object of this study was to investigate the prevalence rate and risk factors of dental erosion for the purpose of providing basic data to an occupational dental health program. Recruited as study subjects were 866 workers in one galvanizing and four battery manufacture factories from March to September, 2000. A questionnaire was administered to workers for information about sociodemographic status, gastric symptoms, dietary habits, medication and symptoms of the body. Clinical oral examinations assessed presence or absence of dental erosion using a classification by Cate. The prevalence rate of dental erosion, the mean number of teeth eroded and eroded teeth(weighted) were calculated. These variables were compared among industry type, factories and location. Logistic regression and multiple regression were analysed for statistical analysis. The results were as follows ; 1. The prevalence rates of dental erosion were Grade 1(25.5%), Grade 2(2.9%), Grade 3(4.5%), Grade 4(1.4%), Grade 5(0.4%) and total(34.7%) in workers exposed to acid. The number of eroded teeth was 1.44?.59 and eroded teeth(weighted) was 2.28?.62. 2. The dental erosion was higher in anterior and upper teeth than in posterior and lower teeth in workers exposed to acid(p<0.05). The dental erosion was higher in upper teeth than in lower teeth in nonexposed workers(p<0.05). 3. In workers exposed to acid, there were significant differences in the prevalence. of dental erosion, eroded teeth and eroded teeth(weighted) among factories(p<0.01). 4. In workers exposed to acid, there were significant differences in the eroded teeth and eroded teeth (weighted) between the two industries (p<0.01). 5. The risk factors for the prevalence of dental erosion were acid(odds ratio: 3.48), service year(odds ratio:1.33) and frequency of tooth brushing by rolling method(odds ratio: 0.73). 6. The risk factors for the number of eroded teeth were acid(B: 0.99), age(B: 0.30) and brittle teeth(B: 1.29), and for the number of eroded teeth(weighted) were acid(B: 1.83), age(B: 0.67) and brittle teeth(B: 1.42). 7. When age and service year were changed as dummy variables, the risk factors for the prevalence of dental erosion were acid(odds ratio: 4.32), service years (5-l0years)(odds ratio: 1.33), age(40-49years)(odds ratio: 1,74), age( <30years) (odds ratio: 0.50) and frequency of tooth brushing(odds ratio: 0.75). 8. When age and service year were changed as dummy variables, the risk factors for eroded teeth were acid(B: 0.98), service years(5-10years)(B: 0.73), age(40-49years)(B: 0.82) and brittle teeth(B: 1.21) and for eroded teeth(weighted) were acid(B: 1.87), age(40-49years)(B: 1.43) and brittle teeth(B: 1.46).

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